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Michel Franc - One of the best experts on this subject based on the ideXlab platform.

  • Sustained speed of kill and repellency of a novel combination of fipronil and permethrin against Ctenocephalides canis flea infestations in dogs
    Parasites & Vectors, 2015
    Co-Authors: Frederic Beugnet, Emilie Bouhsira, Mark Soll, Michel Franc
    Abstract:

    Contexte Ctenocephalides canis est une espèce de puces importante chez les chiens dans plusieurs pays d’Europe. La nouvelle combinaison topique de fipronil et de perméthrine (Frontline Tri-Act®/Frontect®, Merial) a été développée pour lutter contre puces, tiques, moustiques, phlébotomes et mouches piqueuses chez le chien. Etant donné les propriétés répulsives et insecticides de la perméthrine et l’action insecticide du fipronil, l’efficacité de la combinaison contre les puces, dont C. canis , devrait être rapide. L’étude a été conduite afin de mesurer l’efficacité à 1 heure, 6 heures et 24 heures, mais aussi le caractère répulsif de la combinaison fipronil-perméthrine chez les chiens traités par rapport aux chiens non traités. Méthode 12 chiens de race Beagle ont été répartis de manière aléatoire dans l’un des deux groupes sur la base des résultats d’une infestation par les puces avant traitement et durant la phase d’acclimatation. Les chiens du groupe 1 ne sont pas traités alors que les chiens du groupe 2 sont traités une fois à J0. Chaque chien est infesté par 100 puces C. canis adultes à jeun, aux jours 2, 7, 14, 21 et 28. Les chiens sont peignés à la recherche de puces 1 et 6 heures après chaque infestation. Après cet examen, les puces qui restent sur le revêtement de sol de chaque cage sont collectées et comptées. Après les peignages-comptages à 1 heure et 6 heures, toutes les puces vivantes sont replacées sur les chiens. Un dernier comptage par peignage est réalisé pour chaque chien 24 heures après infestation. Résultats Les chiens traités ont eu significativement moins de puces (p ≤ 0,01) que les chiens non traités, à chaque point de comptage. L’efficacité était ≥ 99,1% à 6 h et 24 h après chaque infestation hebdomadaire durant un mois. L’efficacité à 1 heure était également élevée avec 96,5,%, 98,9%, 92,0%, 70,2% et 55,7% aux jours 2, 7, 14, 21 et 28, respectivement. L’efficacité “répulsive”, évaluée sur le revêtement de sol à 1 h, était de 86,5%, 94,9%, 79,5%, 58,4% et 43,9% aux jours 2, 7, 14, 21 et 28, respectivement. Conclusions Cette étude démontre l’effet bénéfique de la combinaison de fipronil et perméthrine contre C. canis , qui apporte une action à la fois insecticide et « répulsive » dès 1 h post infestation, et >99,1% d’efficacité à 6 h pendant un mois. Background Ctenocephalides canis is a major flea species in dogs in several European countries. The new topical combination of fipronil and permethrin (Frontline Tri-Act®/Frontect®, Merial) has been developed to control fleas, ticks, mosquitoes, sandflies and biting flies on dogs. Considering the repellent and insecticidal effects of permethrin and the insecticidal effect of fipronil, the efficacy of the combination against fleas including C. canis was expected to be rapid. The study was conducted to measure the 1-hour, 6-hour and 24-hour efficacy, as well as the repellent activity, of the fipronil-permethrin combination on treated versus untreated dogs. Methods 12 Beagle dogs were randomly allocated to one of two groups based on pre-treatment live flea counts. Dogs in Group 1 remained untreated whereas dogs in Group 2 were treated once on Day 0. Each dog was infested with 100 unfed adult C. canis on Days 2, 7, 14, 21 and 28. Dogs were combed for fleas 1 and 6 h after each infestation. Following this examination, fleas remaining on the liner at the bottom of each cage were collected and counted. All live fleas were placed back on each dog after the 1- and 6-hour counts. A comb-count was performed at 24 h post infestation on all dogs. Results Treated dogs had significantly ( p  ≤ 0.01) lower flea counts than untreated dogs at every time point. The percent efficacy was ≥99.1% at 6 and 24 h after each weekly challenge up to the month. The 1-hour counts also showed good efficacy of 96.5%, 98.9%, 92.0%, 70.2% and 55.7% on Days 2, 7, 14, 21 and 28, respectively. The repellent efficacy, assessed on the liners at 1 h, was 86.5%, 94.9%, 79.5%, 58.4% and 43.9% on Days 2, 7, 14, 21 and 28, respectively. Conclusions This study demonstrates the beneficial effect of the fipronil and permethrin combination against C. canis , providing both a repellent and insecticidal effect as early as 1 h post infestation, and >99.1% efficacy calculated at 6 h during a month.

  • Sustained speed of kill and repellency of a novel combination of fipronil and permethrin against Ctenocephalides canis flea infestations in dogs
    Parasites & vectors, 2015
    Co-Authors: Frederic Beugnet, Emilie Bouhsira, Soll Mark D, Michel Franc
    Abstract:

    Ctenocephalides canis is a major flea species in dogs in several European countries. The new topical combination of fipronil and permethrin (Frontline Tri-Act®/Frontect®, Merial) has been developed to control fleas, ticks, mosquitoes, sandflies and biting flies on dogs. Considering the repellent and insecticidal effects of permethrin and the insecticidal effect of fipronil, the efficacy of the combination against fleas including C. canis was expected to be rapid. The study was conducted to measure the 1-hour, 6-hour and 24-hour efficacy, as well as the repellent activity, of the fipronil-permethrin combination on treated versus untreated dogs. 12 Beagle dogs were randomly allocated to one of two groups based on pre-treatment live flea counts. Dogs in Group 1 remained untreated whereas dogs in Group 2 were treated once on Day 0. Each dog was infested with 100 unfed adult C. canis on Days 2, 7, 14, 21 and 28. Dogs were combed for fleas 1 and 6 h after each infestation. Following this examination, fleas remaining on the liner at the bottom of each cage were collected and counted. All live fleas were placed back on each dog after the 1- and 6-hour counts. A comb-count was performed at 24 h post infestation on all dogs. Treated dogs had significantly (p ≤ 0.01) lower flea counts than untreated dogs at every time point. The percent efficacy was ≥99.1% at 6 and 24 h after each weekly challenge up to the month. The 1-hour counts also showed good efficacy of 96.5%, 98.9%, 92.0%, 70.2% and 55.7% on Days 2, 7, 14, 21 and 28, respectively. The repellent efficacy, assessed on the liners at 1 h, was 86.5%, 94.9%, 79.5%, 58.4% and 43.9% on Days 2, 7, 14, 21 and 28, respectively. This study demonstrates the beneficial effect of the fipronil and permethrin combination against C. canis, providing both a repellent and insecticidal effect as early as 1 h post infestation, and >99.1% efficacy calculated at 6 h during a month.

  • efficacy of dinotefuran permethrin and pyriproxyfen combination spot on on dogs against phlebotomus perniciosus and Ctenocephalides canis
    Parasitology Research, 2013
    Co-Authors: E. Liénard, P. Jacquiet, S. Warin, V. Kaltsatos, Emilie Bouhsira, Michel Franc
    Abstract:

    This study was conducted to evaluate the efficacy of a new topical ectoparasiticidal spot-on containing 4.95 % dinotefuran (w/w), 36.08 % permethrin (w/w) and 0.44 % pyriproxyfen (w/w) (Vectra 3D™, Ceva, Libourne, France) against Portuguese strain of Phlebotomus perniciosus and a French strain of Ctenocephalides canis in dogs. Twelve beagle dogs were exposed for 1 h to 100 P. perniciosus on day 6 for allocation in two groups. One group was treated on day 0, and the other group was the control group. The dogs were exposed for 1 h to 100 P. perniciosus on days 1, 7, 14, 21 and 28. After each sandfly challenge, the same dogs were infested with 100 C. canis. Counts of living fleas were determined 48 h after infestation on days 4, 3, 9, 16, 23 and 30. For sandflies, the anti-feeding effect was 96.9, 99.7, 98.7, 83.5 and 87.0 % on days 1, 7, 14, 21 and 28, respectively. The mortality effect was 97.8, 99.8, 73.7, 27.5 and 39.6 % on days 1, 7, 14, 21 and 28, respectively. At each challenge point, the mortality and anti-feeding effects on sandflies were significantly different between the control and treatment groups (p < 0.05). The adulticidal effect on C. canis remained above 99 % throughout the study period. The results indicate that a combination with dinotefuran, permethrin and pyriproxyfen may be used as an effective part of an overall flea and sandfly control strategy in dogs for monthly use.

  • Evaluation of speed and duration of efficacy of spinosad tablets for treatment and control of Ctenocephalides canis (siphonaptera: pulicidae) infestations in dogs
    Parasite, 2009
    Co-Authors: Michel Franc, Emilie Bouhsira
    Abstract:

    A controlled clinical trial was performed to determine the duration of efficacy of a new oral insecticide formulation of spinosad for the control of experimentally induced Ctenocephalides canis infestations in dogs. Twelve Beagle dogs (two groups of six) were used in the study. Dogs in the treated group received spinosad tablets per os on D0 at the commercial dosage. All dogs were infested with 100 fleas on Days – 7, – 1, 7, 14, 21, 28 and 35. The dogs were combed four hours after each infestation and fleas were counted and replaced on the coat. 24 hours after each infestation fleas were combed, counted and removed. The efficacy of the formulation was calculated four and 24 hours after the treatment and then four and 24 hours after each new infestation. The mean number of fleas on the control dogs was respectively between 65.1 and 83.3 at four hour counts and between 58.3 and 75.3 at 24 hour counts. The product was well tolerated. The treatment controlled the fleas already present on the skin with 81 % efficacy at four hours and 100 % efficacy at 24 hours. For the weekly infestations, the speed of action of the product was high: at four hours the efficacy was 100 % at D7, 96 % at D14, 74 % at D21, 42 % at D28, 12.90 % at D35 and 12.8 % at D42. The efficacy evaluated 24 hours after each infestation was approximately 100 % during three weeks then 90 % at D39, 81.4 % at D36 and 80.4 % at D43. A single dose of the new spinosad tablet formulation should control flea populations in dogs for four weeks as indicated in the claim (evaluation performed at 48 h for the registration). Spinosad tablet is the first product administered per os which acts so long and so quickly against adult fleas.

  • First blood meal of Ctenocephalides canis (Siphonaptera: Pulicidae) on dogs: time to initiation of feeding and duration.
    The Journal of parasitology, 2001
    Co-Authors: Mariechristine Cadiergues, D. Santamarta, X. Mallet, Michel Franc
    Abstract:

    Two experiments were conducted on dogs to evaluate interval to initiation and duration of the first blood meal of Ctenocephalides canis (Curtis). Percentage of fed male and female fleas was calculated for fleas held on dogs for 5, 15, 30, 60 min, 6, and 24 hr. Duration of first blood meal was also measured for individual fleas confined on dogs. When fleas were free in the hair coat, 21.2% had begun blood feeding within 5 min. After 1 hr, 72.5% of fleas had fed. After 6 hr, 95.2% of males and 100% of females had taken a blood meal, and 24 hr after deposition all fleas had fed. There was no significant difference between the 2 sexes. The mean delay between deposition and biting for fleas that began feeding within 15 min was 2 min 52 sec ± 3 min 2 sec for female fleas and 3 min 8 sec ± 2 min 45 sec for males. The mean duration of female and male meals was 5 min 3 sec ± 3 min 41 sec and 6 min 9 sec ± 6 min 8 sec, respectively. There was no significant difference between the 2 sexes. The dog flea took its bloo...

Emilie Bouhsira - One of the best experts on this subject based on the ideXlab platform.

  • Sustained speed of kill and repellency of a novel combination of fipronil and permethrin against Ctenocephalides canis flea infestations in dogs
    Parasites & Vectors, 2015
    Co-Authors: Frederic Beugnet, Emilie Bouhsira, Mark Soll, Michel Franc
    Abstract:

    Contexte Ctenocephalides canis est une espèce de puces importante chez les chiens dans plusieurs pays d’Europe. La nouvelle combinaison topique de fipronil et de perméthrine (Frontline Tri-Act®/Frontect®, Merial) a été développée pour lutter contre puces, tiques, moustiques, phlébotomes et mouches piqueuses chez le chien. Etant donné les propriétés répulsives et insecticides de la perméthrine et l’action insecticide du fipronil, l’efficacité de la combinaison contre les puces, dont C. canis , devrait être rapide. L’étude a été conduite afin de mesurer l’efficacité à 1 heure, 6 heures et 24 heures, mais aussi le caractère répulsif de la combinaison fipronil-perméthrine chez les chiens traités par rapport aux chiens non traités. Méthode 12 chiens de race Beagle ont été répartis de manière aléatoire dans l’un des deux groupes sur la base des résultats d’une infestation par les puces avant traitement et durant la phase d’acclimatation. Les chiens du groupe 1 ne sont pas traités alors que les chiens du groupe 2 sont traités une fois à J0. Chaque chien est infesté par 100 puces C. canis adultes à jeun, aux jours 2, 7, 14, 21 et 28. Les chiens sont peignés à la recherche de puces 1 et 6 heures après chaque infestation. Après cet examen, les puces qui restent sur le revêtement de sol de chaque cage sont collectées et comptées. Après les peignages-comptages à 1 heure et 6 heures, toutes les puces vivantes sont replacées sur les chiens. Un dernier comptage par peignage est réalisé pour chaque chien 24 heures après infestation. Résultats Les chiens traités ont eu significativement moins de puces (p ≤ 0,01) que les chiens non traités, à chaque point de comptage. L’efficacité était ≥ 99,1% à 6 h et 24 h après chaque infestation hebdomadaire durant un mois. L’efficacité à 1 heure était également élevée avec 96,5,%, 98,9%, 92,0%, 70,2% et 55,7% aux jours 2, 7, 14, 21 et 28, respectivement. L’efficacité “répulsive”, évaluée sur le revêtement de sol à 1 h, était de 86,5%, 94,9%, 79,5%, 58,4% et 43,9% aux jours 2, 7, 14, 21 et 28, respectivement. Conclusions Cette étude démontre l’effet bénéfique de la combinaison de fipronil et perméthrine contre C. canis , qui apporte une action à la fois insecticide et « répulsive » dès 1 h post infestation, et >99,1% d’efficacité à 6 h pendant un mois. Background Ctenocephalides canis is a major flea species in dogs in several European countries. The new topical combination of fipronil and permethrin (Frontline Tri-Act®/Frontect®, Merial) has been developed to control fleas, ticks, mosquitoes, sandflies and biting flies on dogs. Considering the repellent and insecticidal effects of permethrin and the insecticidal effect of fipronil, the efficacy of the combination against fleas including C. canis was expected to be rapid. The study was conducted to measure the 1-hour, 6-hour and 24-hour efficacy, as well as the repellent activity, of the fipronil-permethrin combination on treated versus untreated dogs. Methods 12 Beagle dogs were randomly allocated to one of two groups based on pre-treatment live flea counts. Dogs in Group 1 remained untreated whereas dogs in Group 2 were treated once on Day 0. Each dog was infested with 100 unfed adult C. canis on Days 2, 7, 14, 21 and 28. Dogs were combed for fleas 1 and 6 h after each infestation. Following this examination, fleas remaining on the liner at the bottom of each cage were collected and counted. All live fleas were placed back on each dog after the 1- and 6-hour counts. A comb-count was performed at 24 h post infestation on all dogs. Results Treated dogs had significantly ( p  ≤ 0.01) lower flea counts than untreated dogs at every time point. The percent efficacy was ≥99.1% at 6 and 24 h after each weekly challenge up to the month. The 1-hour counts also showed good efficacy of 96.5%, 98.9%, 92.0%, 70.2% and 55.7% on Days 2, 7, 14, 21 and 28, respectively. The repellent efficacy, assessed on the liners at 1 h, was 86.5%, 94.9%, 79.5%, 58.4% and 43.9% on Days 2, 7, 14, 21 and 28, respectively. Conclusions This study demonstrates the beneficial effect of the fipronil and permethrin combination against C. canis , providing both a repellent and insecticidal effect as early as 1 h post infestation, and >99.1% efficacy calculated at 6 h during a month.

  • Sustained speed of kill and repellency of a novel combination of fipronil and permethrin against Ctenocephalides canis flea infestations in dogs
    Parasites & vectors, 2015
    Co-Authors: Frederic Beugnet, Emilie Bouhsira, Soll Mark D, Michel Franc
    Abstract:

    Ctenocephalides canis is a major flea species in dogs in several European countries. The new topical combination of fipronil and permethrin (Frontline Tri-Act®/Frontect®, Merial) has been developed to control fleas, ticks, mosquitoes, sandflies and biting flies on dogs. Considering the repellent and insecticidal effects of permethrin and the insecticidal effect of fipronil, the efficacy of the combination against fleas including C. canis was expected to be rapid. The study was conducted to measure the 1-hour, 6-hour and 24-hour efficacy, as well as the repellent activity, of the fipronil-permethrin combination on treated versus untreated dogs. 12 Beagle dogs were randomly allocated to one of two groups based on pre-treatment live flea counts. Dogs in Group 1 remained untreated whereas dogs in Group 2 were treated once on Day 0. Each dog was infested with 100 unfed adult C. canis on Days 2, 7, 14, 21 and 28. Dogs were combed for fleas 1 and 6 h after each infestation. Following this examination, fleas remaining on the liner at the bottom of each cage were collected and counted. All live fleas were placed back on each dog after the 1- and 6-hour counts. A comb-count was performed at 24 h post infestation on all dogs. Treated dogs had significantly (p ≤ 0.01) lower flea counts than untreated dogs at every time point. The percent efficacy was ≥99.1% at 6 and 24 h after each weekly challenge up to the month. The 1-hour counts also showed good efficacy of 96.5%, 98.9%, 92.0%, 70.2% and 55.7% on Days 2, 7, 14, 21 and 28, respectively. The repellent efficacy, assessed on the liners at 1 h, was 86.5%, 94.9%, 79.5%, 58.4% and 43.9% on Days 2, 7, 14, 21 and 28, respectively. This study demonstrates the beneficial effect of the fipronil and permethrin combination against C. canis, providing both a repellent and insecticidal effect as early as 1 h post infestation, and >99.1% efficacy calculated at 6 h during a month.

  • efficacy of dinotefuran permethrin and pyriproxyfen combination spot on on dogs against phlebotomus perniciosus and Ctenocephalides canis
    Parasitology Research, 2013
    Co-Authors: E. Liénard, P. Jacquiet, S. Warin, V. Kaltsatos, Emilie Bouhsira, Michel Franc
    Abstract:

    This study was conducted to evaluate the efficacy of a new topical ectoparasiticidal spot-on containing 4.95 % dinotefuran (w/w), 36.08 % permethrin (w/w) and 0.44 % pyriproxyfen (w/w) (Vectra 3D™, Ceva, Libourne, France) against Portuguese strain of Phlebotomus perniciosus and a French strain of Ctenocephalides canis in dogs. Twelve beagle dogs were exposed for 1 h to 100 P. perniciosus on day 6 for allocation in two groups. One group was treated on day 0, and the other group was the control group. The dogs were exposed for 1 h to 100 P. perniciosus on days 1, 7, 14, 21 and 28. After each sandfly challenge, the same dogs were infested with 100 C. canis. Counts of living fleas were determined 48 h after infestation on days 4, 3, 9, 16, 23 and 30. For sandflies, the anti-feeding effect was 96.9, 99.7, 98.7, 83.5 and 87.0 % on days 1, 7, 14, 21 and 28, respectively. The mortality effect was 97.8, 99.8, 73.7, 27.5 and 39.6 % on days 1, 7, 14, 21 and 28, respectively. At each challenge point, the mortality and anti-feeding effects on sandflies were significantly different between the control and treatment groups (p < 0.05). The adulticidal effect on C. canis remained above 99 % throughout the study period. The results indicate that a combination with dinotefuran, permethrin and pyriproxyfen may be used as an effective part of an overall flea and sandfly control strategy in dogs for monthly use.

  • efficacy of fipronil amitraz and s methoprene combination spot on for dogs against adult dog fleas Ctenocephalides canis curtis 1826
    Veterinary Parasitology, 2011
    Co-Authors: Emilie Bouhsira, S Yoon, Martine Roques, Coralie Manavella, Solange Vermot, L G Cramer, Catherine Ollagnier, M. Franc
    Abstract:

    Abstract A novel spot-on formulation combining fipronil, amitraz and (S)-methoprene (CERTIFECT™, Merial Limited, GA, USA) was evaluated in adult Beagle dogs in a study to determine its adulticidal efficacy against the dog flea ( Ctenocephalides canis , Curtis, 1826). Sixteen dogs were randomly allocated to treatment groups: 8 dogs served as untreated controls, and 8 dogs were treated once. Treatment consisted of applying a new combination formulation to deliver at least 6.7 mg fipronil/kg body weight (bw), 8.0 mg amitraz/kg bw, and 6.0 mg (S)-methoprene/kg bw. The combination was designed to enhance the efficacy against ticks of the original fipronil/(S)-methoprene combination. Each dog was infested with 100 adult unfed dog fleas within 24 h prior to treatment and then at weekly intervals for 8 weeks after treatment. At 24 h after treatment or after each subsequent infestation, each dog was combed thoroughly to remove live fleas to be counted. A single treatment with CERTIFECT provided excellent knock-down of fleas within 24 h after treatment and controlled re-infestations for up to 7 weeks (efficacy ≥96.5%, p

  • Evaluation of speed and duration of efficacy of spinosad tablets for treatment and control of Ctenocephalides canis (siphonaptera: pulicidae) infestations in dogs
    Parasite, 2009
    Co-Authors: Michel Franc, Emilie Bouhsira
    Abstract:

    A controlled clinical trial was performed to determine the duration of efficacy of a new oral insecticide formulation of spinosad for the control of experimentally induced Ctenocephalides canis infestations in dogs. Twelve Beagle dogs (two groups of six) were used in the study. Dogs in the treated group received spinosad tablets per os on D0 at the commercial dosage. All dogs were infested with 100 fleas on Days – 7, – 1, 7, 14, 21, 28 and 35. The dogs were combed four hours after each infestation and fleas were counted and replaced on the coat. 24 hours after each infestation fleas were combed, counted and removed. The efficacy of the formulation was calculated four and 24 hours after the treatment and then four and 24 hours after each new infestation. The mean number of fleas on the control dogs was respectively between 65.1 and 83.3 at four hour counts and between 58.3 and 75.3 at 24 hour counts. The product was well tolerated. The treatment controlled the fleas already present on the skin with 81 % efficacy at four hours and 100 % efficacy at 24 hours. For the weekly infestations, the speed of action of the product was high: at four hours the efficacy was 100 % at D7, 96 % at D14, 74 % at D21, 42 % at D28, 12.90 % at D35 and 12.8 % at D42. The efficacy evaluated 24 hours after each infestation was approximately 100 % during three weeks then 90 % at D39, 81.4 % at D36 and 80.4 % at D43. A single dose of the new spinosad tablet formulation should control flea populations in dogs for four weeks as indicated in the claim (evaluation performed at 48 h for the registration). Spinosad tablet is the first product administered per os which acts so long and so quickly against adult fleas.

José A. Oteo - One of the best experts on this subject based on the ideXlab platform.

Philippe Parola - One of the best experts on this subject based on the ideXlab platform.

  • First molecular detection of Rickettsia felis in fleas from Algeria. Am J Trop Med Hyg. 2006;74:532–5. Address for correspondence: Jeremie Gilles, Department of Entomology, University of Kentucky, S225 Agricultural Science Center North
    2015
    Co-Authors: Idir Bitam, Philippe Parola, Katharina Dittmar, De La Cruz, Koutaro Matsumoto
    Abstract:

    Abstract. Fleas collected in Algeria in the district of Oran between July and September 2003 were tested by polymerase chain reaction for the presence of Rickettsia spp. DNA using primers amplifying gltA and OmpA genes. Two gltA sequences identical to those of an emerging pathogen, Rickettsia felis, were detected including i) R. felis California 2 in Ctenocephalides canis from rodents and ii) R. felis RF2125 in Archeopsylla erinacei from hedgehogs

  • Identification of flea species using MALDI-TOF/MS
    Comparative Immunology Microbiology and Infectious Diseases, 2014
    Co-Authors: Amina Yssouf, Gilles Audoly, Hamza Leulmi, Lionel Almeras, Cristina Socolovschi, Tahar Kernif, Idir Bitam, Philippe Parola
    Abstract:

    a b s t r a c t In the present study, a molecular proteomics (MALDI-TOF/MS) approach was used as a tool for identifying flea vectors. We measured the MS spectra from 38 flea specimens of 5 species including Ctenocephalides felis, Ctenocephalides canis, Archaeopsylla erinacei, Xenop- sylla cheopis and Stenoponia tripectinata. A blind test performed with 24 specimens from species included in a library spectral database confirmed that MALDI-TOF/MS is an effective tool for discriminating flea species. Although fresh and 70% ethanol-conserved samples sub- jected to MALDI-TOF/MS in blind tests were correctly classified, only MS spectra of quality from fresh specimens were sufficient for accurate and significant identification. A clus- ter analysis highlighted that the MALDI Biotyper can be used for studying the phylogeny of fleas.

  • Molecular detection of Rickettsia typhi and Rickettsia felis in fleas from Algeria.
    Clinical Microbiology and Infection, 2009
    Co-Authors: Idir Bitam, B Baziz, Tahar Kernif, Philippe Parola, Zoubir Harrat, Didier Raoult
    Abstract:

    With the exception of the rat flea Xenopsylla cheopis and Yersinia pestis, the agent of plague, fleas and human flea-borne infections have been scarcely studied in Algeria [1]. Murine typhus, a typhus group rickettsiosis caused by Rickettsia typhi and transmitted by X. cheopis, is also known to occur in Algeria, but cases are poorly documented [2,3]. However, recently, fleas collected in Algeria, in the district of Oran, between July and September 2003 (Ctenocephalides canis from rodents, and Archeopsylla erinacei from hedgehogs) were tested by PCR for the presence of Rickettsia spp., and were shown to harbour an emerging pathogen, Rickettsia felis [4]. In this work, in an effort to identify the possible aetiological agents and vectors for rickettsiosis affecting humans in Algeria, we analysed more fleas collected from rodents trapped in peridomestic areas for evidence of rickettsial infection.

  • first molecular detection of rickettsia felis in fleas from algeria
    American Journal of Tropical Medicine and Hygiene, 2006
    Co-Authors: Idir Bitam, Katharina Dittmar De La Cruz, Koutaro Matsumoto, B Baziz, Philippe Parola, Jean-marc Rolain, Miloud Belkaid
    Abstract:

    Fleas collected in Algeria in the district of Oran between July and September 2003 were tested by polymerase chain reaction for the presence of Rickettsia spp. DNA using primers amplifying gltA and OmpA genes. Two gltA sequences identical to those of an emerging pathogen, Rickettsia felis, were detected including i) R. felis California 2 in Ctenocephalides canis from rodents and ii) R. felis RF2125 in Archeopsylla erinacei from hedgehogs.

  • identification of rickettsia spp and bartonella spp in fleas from the thai myanmar border
    Annals of the New York Academy of Sciences, 2003
    Co-Authors: O Y Sanogo, Gilles Chauvancy, Zaher Zeaiter, Kriangkrai Lerdthusnee, R. S. Miller, Jeanpaul Gonzalez, Steven R. Telford, Philippe Parola, Chansuda Wongsrichanalai
    Abstract:

    : During a survey for possible rickettsial vectors in villages of the central part of the Thai-Myanmar border from September 2001 to February 2002, four species of fleas were collected from common peridomestic animals. All fleas were tested by PCR to detect DNA of bacteria of the genera Rickettsia (gltA and ompB genes) and Bartonella (ITS and ftsZ genes). Sequencing of PCR-amplified products was done using gltA fragments for Rickettsia and ftsZ fragments for Bartonella. Two genotypes related to Rickettsia felis were identified in three Ctenocephalides canis and one C. felis specimen. Further, the following Bartonella spp. were detected: Bartonella henselae in two C. felis specimens; Bartonella clarridgeiae in three C. felis specimens; and a new Bartonella genotype in one Nosopsylla fasciatus specimen. Rickettsia and Bartonella may be frequently detected in fleas infesting peridomestic animals from the western border of Thailand.

Miguel Ramos Parra - One of the best experts on this subject based on the ideXlab platform.

  • seasonal occurrence of Ctenocephalides felis felis and Ctenocephalides canis siphonaptera pulicidae infesting dogs and cats in an urban area in cuernavaca mexico
    Journal of Medical Entomology, 2001
    Co-Authors: Carlos Cruzvazquez, Eduardo Castro Gamez, Magdalena Parada Fernandez, Miguel Ramos Parra
    Abstract:

    Abstract The seasonal occurrence of Ctenocephalides felis felis (Bouche) and Ctenocephalides canis (Curtis) infestation on dogs and cats in Cuernavaca City in Mexico, was determined by examining 1,803 dogs and 517 cats at two veterinary clinics during 1995–1997. The overall flea infestation was 30.3 and 30.1% for dogs and cats, respectively. There were no significant differences (P > 0.05) in percentage of infestation among years for both hosts. The infestation was somewhat higher in spring, summer, and autumn than in winter, but no statistical differences was found among seasons (P > 0.05) for both pets. No relationship existed between percentage of flea infestation and temperature or rainfall among seasons. On dogs, 81.1% were infested with only C. felis felis, 16.8% with C. canis, and 2% had both flea species; whereas 92.3% of the cats were infested with C. felis felis and 7.7% with C. felis felis and C. canis. The cat flea was the most prevalent flea species found other than C. canis; no other species...