The Experts below are selected from a list of 40683 Experts worldwide ranked by ideXlab platform
Eric G. Wright - One of the best experts on this subject based on the ideXlab platform.
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pre irradiation of tissue Culture Flasks leads to diminished stem and progenitor cell production in long term bone marrow Cultures
Cell Proliferation, 1993Co-Authors: P. Rooney, Eric G. WrightAbstract:. Empty plastic tissue Culture Flasks were exposed to X-irradiation doses of 0.3–10.0 Gy, prior to the establishment of long-term bone marrow Cultures. During the course of a 10 week Culture period, all irradiated plastic Flasks exhibited a dramatic decrease in the number of both haemopoietic stem cells and myeloid progenitor cells, in the non-adherent layer, when compared with controls. This decrease was not due to a decrease in the number of non-adherent cells produced. Histological examination of non-adherent cells showed an increase in mature granulocytic cells with few blast cells. Morphologically, the adherent layers of irradiated Flasks demonstrated a delay in appearance or absence of fat cell production. X-irradiation of glass tissue Culture Flasks had no deleterious effect.
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Pre‐irradiation of tissue Culture Flasks leads to diminished stem and progenitor cell production in long‐term bone marrow Cultures
Cell proliferation, 1993Co-Authors: P. Rooney, Eric G. WrightAbstract:. Empty plastic tissue Culture Flasks were exposed to X-irradiation doses of 0.3–10.0 Gy, prior to the establishment of long-term bone marrow Cultures. During the course of a 10 week Culture period, all irradiated plastic Flasks exhibited a dramatic decrease in the number of both haemopoietic stem cells and myeloid progenitor cells, in the non-adherent layer, when compared with controls. This decrease was not due to a decrease in the number of non-adherent cells produced. Histological examination of non-adherent cells showed an increase in mature granulocytic cells with few blast cells. Morphologically, the adherent layers of irradiated Flasks demonstrated a delay in appearance or absence of fat cell production. X-irradiation of glass tissue Culture Flasks had no deleterious effect.
P. Rooney - One of the best experts on this subject based on the ideXlab platform.
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pre irradiation of tissue Culture Flasks leads to diminished stem and progenitor cell production in long term bone marrow Cultures
Cell Proliferation, 1993Co-Authors: P. Rooney, Eric G. WrightAbstract:. Empty plastic tissue Culture Flasks were exposed to X-irradiation doses of 0.3–10.0 Gy, prior to the establishment of long-term bone marrow Cultures. During the course of a 10 week Culture period, all irradiated plastic Flasks exhibited a dramatic decrease in the number of both haemopoietic stem cells and myeloid progenitor cells, in the non-adherent layer, when compared with controls. This decrease was not due to a decrease in the number of non-adherent cells produced. Histological examination of non-adherent cells showed an increase in mature granulocytic cells with few blast cells. Morphologically, the adherent layers of irradiated Flasks demonstrated a delay in appearance or absence of fat cell production. X-irradiation of glass tissue Culture Flasks had no deleterious effect.
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Pre‐irradiation of tissue Culture Flasks leads to diminished stem and progenitor cell production in long‐term bone marrow Cultures
Cell proliferation, 1993Co-Authors: P. Rooney, Eric G. WrightAbstract:. Empty plastic tissue Culture Flasks were exposed to X-irradiation doses of 0.3–10.0 Gy, prior to the establishment of long-term bone marrow Cultures. During the course of a 10 week Culture period, all irradiated plastic Flasks exhibited a dramatic decrease in the number of both haemopoietic stem cells and myeloid progenitor cells, in the non-adherent layer, when compared with controls. This decrease was not due to a decrease in the number of non-adherent cells produced. Histological examination of non-adherent cells showed an increase in mature granulocytic cells with few blast cells. Morphologically, the adherent layers of irradiated Flasks demonstrated a delay in appearance or absence of fat cell production. X-irradiation of glass tissue Culture Flasks had no deleterious effect.
Anna-lena Sunesson - One of the best experts on this subject based on the ideXlab platform.
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PRODUCTION OF VOLATILE METABOLITES FROM STREPTOMYCES ALBIDOFLAVUS CULTIVATED ON GYPSUM BOARD AND TRYPTONE GLUCOSE EXTRACT AGAR—INFLUENCE OF TEMPERATURE, OXYGEN AND CARBON DIOXIDE LEVELS
Annals of Occupational Hygiene, 1997Co-Authors: Anna-lena Sunesson, Carl-axel Nilsson, Göran Blomquist, Ji Rolf Carlson, Barbro AnderssonAbstract:Streptomyces albidoflavus was cultivated on tryptone glucose extract agar (TGEA) and gypsum board in Culture Flasks. The medium, the cultivation temperature, and the oxygen and carbon dioxide levels in the supplied air were varied multivariately. Air samples from the Cultures were adsorbed on Tenax TA, analysed with the use of thermal desorption-cold trap injection gas chromatography, and identified by mass spectrometry. Alcohols, ketones, terpenes and terpene derivatives, for example geosmin, were produced on both media. On TGEA, sulphur compounds were the dominating products, dimethyl disulphide being produced in major amounts. The medium and the temperature exerted the highest influence on production, but the oxygen and carbon dioxide levels also affected the amounts of some metabolites produced.
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Identification of volatile metabolites from five fungal species cultivated on two media.
Applied and Environmental Microbiology, 1995Co-Authors: Anna-lena Sunesson, Carl-axel Nilsson, Barbro Andersson, Göran Blomquist, Wouter H. J. Vaes, R. CarlsonAbstract:Five fungal species, Aspergillus versicolor, Penicillium commune, Cladosporium cladosporioides, Paecilomyces variotii, and Phialophora fastigiata, were cultivated on two media, malt extract agar and dichloran glycerol agar. Culture Flasks provided with inlet and outlet tubes were used and purified, and humidified air was constantly led through the Flasks. Air samples from the Cultures were sorbed on Tenax GR and analyzed by thermal desorption-gas chromatography. The produced volatile metabolites were analyzed by mass spectrometry. Various hydrocarbons, alcohols, ketones, ethers, esters, sulfur-containing compounds, and terpenes were identified. The most commonly produced substances were 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, 3-methylfuran, and dimethyl disulfide. The production was highly dependent on both medium and species.
Barbro Andersson - One of the best experts on this subject based on the ideXlab platform.
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PRODUCTION OF VOLATILE METABOLITES FROM STREPTOMYCES ALBIDOFLAVUS CULTIVATED ON GYPSUM BOARD AND TRYPTONE GLUCOSE EXTRACT AGAR—INFLUENCE OF TEMPERATURE, OXYGEN AND CARBON DIOXIDE LEVELS
Annals of Occupational Hygiene, 1997Co-Authors: Anna-lena Sunesson, Carl-axel Nilsson, Göran Blomquist, Ji Rolf Carlson, Barbro AnderssonAbstract:Streptomyces albidoflavus was cultivated on tryptone glucose extract agar (TGEA) and gypsum board in Culture Flasks. The medium, the cultivation temperature, and the oxygen and carbon dioxide levels in the supplied air were varied multivariately. Air samples from the Cultures were adsorbed on Tenax TA, analysed with the use of thermal desorption-cold trap injection gas chromatography, and identified by mass spectrometry. Alcohols, ketones, terpenes and terpene derivatives, for example geosmin, were produced on both media. On TGEA, sulphur compounds were the dominating products, dimethyl disulphide being produced in major amounts. The medium and the temperature exerted the highest influence on production, but the oxygen and carbon dioxide levels also affected the amounts of some metabolites produced.
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Identification of volatile metabolites from five fungal species cultivated on two media.
Applied and Environmental Microbiology, 1995Co-Authors: Anna-lena Sunesson, Carl-axel Nilsson, Barbro Andersson, Göran Blomquist, Wouter H. J. Vaes, R. CarlsonAbstract:Five fungal species, Aspergillus versicolor, Penicillium commune, Cladosporium cladosporioides, Paecilomyces variotii, and Phialophora fastigiata, were cultivated on two media, malt extract agar and dichloran glycerol agar. Culture Flasks provided with inlet and outlet tubes were used and purified, and humidified air was constantly led through the Flasks. Air samples from the Cultures were sorbed on Tenax GR and analyzed by thermal desorption-gas chromatography. The produced volatile metabolites were analyzed by mass spectrometry. Various hydrocarbons, alcohols, ketones, ethers, esters, sulfur-containing compounds, and terpenes were identified. The most commonly produced substances were 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, 3-methylfuran, and dimethyl disulfide. The production was highly dependent on both medium and species.
Eric M. Poeschla - One of the best experts on this subject based on the ideXlab platform.
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Production and harvest of feline immunodeficiency virus-based lentiviral vector from cells grown in T75 tissue-Culture Flasks.
Cold Spring Harbor protocols, 2012Co-Authors: Dyana T. Saenz, Román Barraza, Nils A. Loewen, Wulin Teo, Eric M. PoeschlaAbstract:Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes the production and harvesting of vector from cells grown in T75 tissue-Culture Flasks. The methods are for production for basic science laboratory use and in vivo experimentation. They do not meet standards for clinical-grade (good manufacturing practice [GMP]) production.
