The Experts below are selected from a list of 303 Experts worldwide ranked by ideXlab platform
E. Carraro - One of the best experts on this subject based on the ideXlab platform.
-
Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and Culture Methods
Journal of applied microbiology, 2009Co-Authors: Bonetta, Si. Bonetta, E. Ferretti, F. Balocco, E. CarraroAbstract:Aims: This study was designed to define the extent of water contamination by Legionella pneumophila of certain Italian hotels and to compare quantitative real-time PCR with the conventional Culture Method. Methods and Results: Nineteen Italian hotels of different sizes were investigated. In each hotel three hot water samples (boiler, room showers, recycling) and one cold water sample (inlet) were collected. Physico-chemical parameters were also analysed. Legionella pneumophila was detected in 42% and 74% of the hotels investigated by the Culture Method and by real-time PCR, respectively. In 21% of samples analysed by the Culture Method, a concentration of >104 CFU l−1 was found, and Leg. pneumophila serogroup 1 was isolated from 10·5% of the hotels. The presence of Leg. pneumophila was significantly influenced by water sample temperature, while no association with water hardness or residual-free chlorine was found. Conclusions: This study showed a high percentage of buildings colonized by Leg. pneumophila. Moreover, real-time PCR proved to be sensitive enough to detect lower levels of contamination than the Culture Method. Significance and Impact of the Study: This study indicates that the Italian hotels represent a possible source of risk for Legionnaires’ disease and confirms the sensitivity of the molecular Method. To our knowledge, this is the first report to demonstrate Legionella contamination in Italian hotels using real-time PCR and Culture Methods.
Yasuhiko Tabata - One of the best experts on this subject based on the ideXlab platform.
-
Proliferation, osteogenic differentiation, and distribution of rat bone marrow stromal cells in nonwoven fabrics by different Culture Methods.
Tissue engineering. Part A, 2008Co-Authors: Norihisa Ichinohe, Tomoaki Takamoto, Yasuhiko TabataAbstract:The proliferation, osteogenic differentiation, and distribution patterns of stromal cells from rat bone marrow were investigated in a three-dimensional nonwoven fabric of polyethylene terephthalate fiber by the static, agitated, and stirred Culture Methods; stirring speeds were 10, 50, and 100 rpm in the stirred Culture Method. The Culture Method affected the time profile of proliferation and osteogenic differentiation of cells or their distribution in the fabric. The extent of cell proliferation and osteogenic differentiation became higher in order of the stirred at 100 rpm = the stirred at 50 rpm > the stirred at 10 rpm > the agitated > the static Methods. In addition, the cells were more uniformly proliferated in the fabric by the stirred Culture Method with time than they were proliferated in the fabric by other Methods. The alkaline phosphatase (ALP) activity and calcium content were higher for cells Cultured by the stirred Culture Method than those Cultured by other Methods. The total ALP activity, ...
-
ectopic bone formation in collagen sponge self assembled peptide amphiphile nanofibers hybrid scaffold in a perfusion Culture bioreactor
Biomaterials, 2006Co-Authors: Hossein Hosseinkhani, Mohsen Hosseinkhani, Furong Tian, Hisatoshi Kobayashi, Yasuhiko TabataAbstract:Abstract The objective of this study was to enhance ectopic bone formation in a three-dimensional (3-D) hybrid scaffold in combination with bioreactor perfusion Culture system. The hybrid scaffold consists of two biomaterials, a hydrogel formed through self-assembly of peptide–amphiphile (PA) with cell suspensions in media, and a collagen sponge reinforced with poly(glycolic acid) (PGA) fiber incorporation. PA was synthesized by standard solid-phase chemistry that ends with the alkylation of the NH 2 terminus of the peptide. A 3-D network of nanofibers was formed by mixing cell suspensions in media with dilute aqueous solution of PA. Scanning electron microscopy (SEM) observation revealed the formation of fibrous assemblies with an extremely high aspect ratio and high surface areas. Osteogenic differentiation of mesenchymal stem cells (MSC) in the hybrid scaffold was greatly influenced by the perfusion Culture Method compared with static Culture Method. When the osteoinduction activity of hybrid scaffold was studied following the implantation into the back subcutis of rats in terms of histological and biochemical examinations, significantly homogeneous bone formation was histologically observed throughout the hybrid scaffolds when perfusion Culture was used compared with static Culture Method. The level of alkaline phosphatase activity and osteocalcin content at the implanted sites of hybrid scaffolds were significantly high for the perfusion group compared with those in static Culture Method. We conclude that combination of MSC-seeded hybrid scaffold and the perfusion Method was promising to enhance in vitro osteogenic differentiation of MSC and in vivo ectopic bone formation.
-
Influence of Culture Method on the proliferation and osteogenic differentiation of human adipo-stromal cells in nonwoven fabrics.
Tissue engineering, 2004Co-Authors: Kaori Yasuda, Sachiko Inoue, Yasuhiko TabataAbstract:The initial attachment, proliferation, and osteogenic differentiation of stromal cells from human fat tissue were investigated in three-dimensional nonwoven fabrics prepared from polyethylene terephthalate (PET) fiber with different diameters. The largest number of cells initially attached was observed in the nonwoven fabrics prepared from PET fiber with a diameter of 22.0 microm, irrespective of fabric porosity. The number of cells attached was larger and the cells were distributed more homogeneously in the fabrics by the agitated seeding Method than by the static seeding Method. The Culture Method depended on the time profile of cell proliferation. Cell proliferation improved in the following order: stirred (spinner flask) Culture Method > agitated Culture Method > static Culture Method. In addition, cells proliferated homogeneously in fabrics by the stirred Culture Method. When evaluated as a measurement of cell osteogenic differentiation, the activity of alkaline phosphatase (ALP) was not influenced by the diameter of fabrics. The static Culture Method tended to enable cells to enhance ALP activity, in contrast with the stirred and agitated Culture Methods. It is concluded that fabric fiber diameter and Culture Method greatly affected the proliferation and differentiation of cells in nonwoven fabrics.
Bonetta - One of the best experts on this subject based on the ideXlab platform.
-
Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and Culture Methods
Journal of applied microbiology, 2009Co-Authors: Bonetta, Si. Bonetta, E. Ferretti, F. Balocco, E. CarraroAbstract:Aims: This study was designed to define the extent of water contamination by Legionella pneumophila of certain Italian hotels and to compare quantitative real-time PCR with the conventional Culture Method. Methods and Results: Nineteen Italian hotels of different sizes were investigated. In each hotel three hot water samples (boiler, room showers, recycling) and one cold water sample (inlet) were collected. Physico-chemical parameters were also analysed. Legionella pneumophila was detected in 42% and 74% of the hotels investigated by the Culture Method and by real-time PCR, respectively. In 21% of samples analysed by the Culture Method, a concentration of >104 CFU l−1 was found, and Leg. pneumophila serogroup 1 was isolated from 10·5% of the hotels. The presence of Leg. pneumophila was significantly influenced by water sample temperature, while no association with water hardness or residual-free chlorine was found. Conclusions: This study showed a high percentage of buildings colonized by Leg. pneumophila. Moreover, real-time PCR proved to be sensitive enough to detect lower levels of contamination than the Culture Method. Significance and Impact of the Study: This study indicates that the Italian hotels represent a possible source of risk for Legionnaires’ disease and confirms the sensitivity of the molecular Method. To our knowledge, this is the first report to demonstrate Legionella contamination in Italian hotels using real-time PCR and Culture Methods.
F. Balocco - One of the best experts on this subject based on the ideXlab platform.
-
Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and Culture Methods
Journal of applied microbiology, 2009Co-Authors: Bonetta, Si. Bonetta, E. Ferretti, F. Balocco, E. CarraroAbstract:Aims: This study was designed to define the extent of water contamination by Legionella pneumophila of certain Italian hotels and to compare quantitative real-time PCR with the conventional Culture Method. Methods and Results: Nineteen Italian hotels of different sizes were investigated. In each hotel three hot water samples (boiler, room showers, recycling) and one cold water sample (inlet) were collected. Physico-chemical parameters were also analysed. Legionella pneumophila was detected in 42% and 74% of the hotels investigated by the Culture Method and by real-time PCR, respectively. In 21% of samples analysed by the Culture Method, a concentration of >104 CFU l−1 was found, and Leg. pneumophila serogroup 1 was isolated from 10·5% of the hotels. The presence of Leg. pneumophila was significantly influenced by water sample temperature, while no association with water hardness or residual-free chlorine was found. Conclusions: This study showed a high percentage of buildings colonized by Leg. pneumophila. Moreover, real-time PCR proved to be sensitive enough to detect lower levels of contamination than the Culture Method. Significance and Impact of the Study: This study indicates that the Italian hotels represent a possible source of risk for Legionnaires’ disease and confirms the sensitivity of the molecular Method. To our knowledge, this is the first report to demonstrate Legionella contamination in Italian hotels using real-time PCR and Culture Methods.
E. Ferretti - One of the best experts on this subject based on the ideXlab platform.
-
Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and Culture Methods
Journal of applied microbiology, 2009Co-Authors: Bonetta, Si. Bonetta, E. Ferretti, F. Balocco, E. CarraroAbstract:Aims: This study was designed to define the extent of water contamination by Legionella pneumophila of certain Italian hotels and to compare quantitative real-time PCR with the conventional Culture Method. Methods and Results: Nineteen Italian hotels of different sizes were investigated. In each hotel three hot water samples (boiler, room showers, recycling) and one cold water sample (inlet) were collected. Physico-chemical parameters were also analysed. Legionella pneumophila was detected in 42% and 74% of the hotels investigated by the Culture Method and by real-time PCR, respectively. In 21% of samples analysed by the Culture Method, a concentration of >104 CFU l−1 was found, and Leg. pneumophila serogroup 1 was isolated from 10·5% of the hotels. The presence of Leg. pneumophila was significantly influenced by water sample temperature, while no association with water hardness or residual-free chlorine was found. Conclusions: This study showed a high percentage of buildings colonized by Leg. pneumophila. Moreover, real-time PCR proved to be sensitive enough to detect lower levels of contamination than the Culture Method. Significance and Impact of the Study: This study indicates that the Italian hotels represent a possible source of risk for Legionnaires’ disease and confirms the sensitivity of the molecular Method. To our knowledge, this is the first report to demonstrate Legionella contamination in Italian hotels using real-time PCR and Culture Methods.
