The Experts below are selected from a list of 9603 Experts worldwide ranked by ideXlab platform
Tetsuya Tateishi - One of the best experts on this subject based on the ideXlab platform.
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Static and dynamic mechanical properties of extracellular matrix synthesized by Cultured Chondrocytes
Materials Science and Engineering: C, 2004Co-Authors: Shogo Miyata, Katsuko S. Furukawa, Takashi Ushida, Yasuo Nitta, Tetsuya TateishiAbstract:Abstract Recently, an approach to restore cartilage defects, culturing autologous Chondrocytes in vitro to create a three-dimensional tissue and then implanting the Cultured tissue, has been developed. In this kind of approach, it is important to study whether the extracellular matrixes synthesized by Cultured Chondrocytes have appropriate mechanical property. In this study, we attempted to evaluate in detail mechanical properties of the extracellular matrix synthesized by Chondrocytes in vitro. Therefore, we Cultured bovine Chondrocytes in agarose gel and assessed the changes in not only static but also dynamic mechanical properties during long-term culture. In addition, to assess the mechanical property of the extracellular matrix synthesized by Chondrocytes only, we calculated the volume ratio of the extracellular matrix in agarose gel from the cross section and estimated the static Young's modulus of the extracellular matrix synthesized by Cultured Chondrocytes based on the Rule of Mixture . As a result, both the equilibrium aggregate modulus and dynamic Young's modulus of chondrocyte/agarose disks increased with time. The value of calculated modulus was larger than that of the pericellular matrix of cartilage explant. In conclusion, the biomechanical property of chondrocyte/agarose constructs and the matrix synthesized by Chondrocytes were assessed in detail.
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Proteoglycan synthesis by Chondrocytes Cultured under hydrostatic pressure and perfusion
Materials Science and Engineering: C, 1998Co-Authors: Toshimi Murata, Takashi Ushida, Shuichi Mizuno, Tetsuya TateishiAbstract:The direct effect of hydrostatic pressure on proteoglycan synthesis in Cultured Chondrocytes was examined using a specially designed system for applying hydrostatic pressure. Chondrocytes were isolated from bovine articular cartilage and seeded on cover glasses coated with type I collagen. The cover glasses were suspended in a column which allowed continuous positive hydrostatic pressure at 2.8 MPa and medium perfusion at 0.3 ml/min, during 96 h. Accumulations of keratan sulfate proteoglycan and chondroitin 4-sulfate proteoglycan increased 1.2- and 1.8-fold, respectively, compared with static condition. These results suggest that continuous hydrostatic pressure and medium perfusion promote keratan sulfate proteoglycan and chondroitin 4-sulfate proteoglycan syntheses by Cultured Chondrocytes.
Daniel B.f. Saris - One of the best experts on this subject based on the ideXlab platform.
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Matrix-Applied Characterized Autologous Cultured Chondrocytes Versus Microfracture: Five-Year Follow-up of a Prospective Randomized Trial.
The American journal of sports medicine, 2018Co-Authors: Mats Brittberg, David Recker, John Ilgenfritz, Daniel B.f. SarisAbstract:Matrix-based cell therapy improves surgical handling, increases patient comfort, and allows for expanded indications with better reliability within the knee joint. Five-year efficacy and safety of autologous Cultured Chondrocytes on porcine collagen membrane (MACI) versus microfracture for treating cartilage defects have not yet been reported from any randomized controlled clinical trial. To examine the clinical efficacy and safety results at 5 years after treatment with MACI and compare these with the efficacy and safety of microfracture treatment for symptomatic cartilage defects of the knee. Randomized controlled trial; Level of evidence, 1. This article describes the 5-year follow-up of the SUMMIT (Superiority of MACI Implant Versus Microfracture Treatment) clinical trial conducted at 14 study sites in Europe. All 144 patients who participated in SUMMIT were eligible to enroll; analyses of the 5-year data were performed with data from patients who signed informed consent and continued in the Extension study. Of the 144 patients randomized in the SUMMIT trial, 128 signed informed consent and continued observation in the Extension study: 65 MACI (90.3%) and 63 microfracture (87.5%). The improvements in Knee injury and Osteoarthritis Outcome Score (KOOS) Pain and Function domains previously described were maintained over the 5-year follow-up. Five years after treatment, the improvement in MACI over microfracture in the co-primary endpoint of KOOS pain and function was maintained and was clinically and statistically significant ( P = .022). Improvements in activities of daily living remained statistically significantly better ( P = .007) in MACI patients, with quality of life and other symptoms remaining numerically higher in MACI patients but losing statistical significance relative to the results of the SUMMIT 2-year analysis. Magnetic resonance imaging (MRI) evaluation of structural repair was performed in 120 patients at year 5. As in the 2-year SUMMIT (MACI00206) results, the MRI evaluation showed improvement in defect filling for both treatments; however, no statistically significant differences were noted between treatment groups. Symptomatic cartilage knee defects 3 cm2 or larger treated with MACI were clinically and statistically significantly improved at 5 years compared with microfracture treatment. No remarkable adverse events or safety issues were noted in this heterogeneous patient population.
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Matrix-Applied Characterized Autologous Cultured Chondrocytes Versus Microfracture Two-Year Follow-up of a Prospective Randomized Trial
The American journal of sports medicine, 2014Co-Authors: Daniel B.f. Saris, Andrew Price, Wojciech Widuchowski, Marion Bertrand-marchand, Jacob Caron, Jon Olav Drogset, Pieter J. Emans, Ales Podskubka, A.i. Tsuchida, Sven KiliAbstract:Background:Randomized controlled trials studying the efficacy and safety of matrix-applied characterized autologous Cultured Chondrocytes (MACI) versus microfracture (MFX) for treating cartilage defects are limited.Purpose:To compare the clinical efficacy and safety of MACI versus MFX in the treatment of patients with symptomatic cartilage defects of the knee.Study Design:Randomized controlled clinical trial; Level of evidence, 1.Methods:Patients enrolled in the SUMMIT (Demonstrate the Superiority of MACI implant to Microfracture Treatment) trial had ≥1 symptomatic focal cartilage defect (Outerbridge grade III or IV; ≥3 cm2) of the femoral condyles or trochlea, with a baseline Knee Injury and Osteoarthritis Outcome Score (KOOS) pain value
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matrix applied characterized autologous Cultured Chondrocytes versus microfracture two year follow up of a prospective randomized trial
American Journal of Sports Medicine, 2014Co-Authors: Daniel B.f. Saris, Wojciech Widuchowski, Jacob Caron, Jon Olav Drogset, Pieter J. Emans, Ales Podskubka, A.i. Tsuchida, A J Price, Marion Bertrandmarchand, Sven KiliAbstract:Background:Randomized controlled trials studying the efficacy and safety of matrix-applied characterized autologous Cultured Chondrocytes (MACI) versus microfracture (MFX) for treating cartilage defects are limited.Purpose:To compare the clinical efficacy and safety of MACI versus MFX in the treatment of patients with symptomatic cartilage defects of the knee.Study Design:Randomized controlled clinical trial; Level of evidence, 1.Methods:Patients enrolled in the SUMMIT (Demonstrate the Superiority of MACI implant to Microfracture Treatment) trial had ≥1 symptomatic focal cartilage defect (Outerbridge grade III or IV; ≥3 cm2) of the femoral condyles or trochlea, with a baseline Knee Injury and Osteoarthritis Outcome Score (KOOS) pain value <55. The co–primary efficacy endpoint was the change in the KOOS pain and function subscores from baseline to 2 years. Histological evaluation and magnetic resonance imaging (MRI) assessments of structural repair tissue, treatment failure, the remaining 3 KOOS subscales,...
Masakazu Kuriwaka - One of the best experts on this subject based on the ideXlab platform.
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effects of low intensity pulsed ultrasound on proliferation and chondroitin sulfate synthesis of Cultured Chondrocytes embedded in atelocollagen gel
Journal of Biomedical Materials Research, 2002Co-Authors: T Nishikori, Kenichi Katsube, H Kataoka, Kenzo Kawasaki, Shunji Maniwa, Yuji Uchio, Mitsuo Ochi, Masakazu KuriwakaAbstract:The effects of low-intensity pulsed ultrasound (US) on the proliferation and chondroitin sulfate synthesis of Cultured Chondrocytes embedded in Atelocollagen® gel in vitro were examined. Articular cartilage was harvested from the hip, knee, and shoulder joints of 10-week-old Japanese white rabbits. Chondrocytes isolated by collagenase digestion were embedded in type I collagen gel, Atelocollagen gel, and were Cultured in Dulbecco's modified eagle's medium for 3 weeks. The US apparatus, SAFHS® , was used to deliver an ultrasound signal with spatial and temporal average intensities of 30 mW/cm2 (US group). The frequency was 1.5 MHz with a 200-microsecond tone burst repeated at 1.0 kHz. US treatments were administered for 20 min per day under culture dishes, with the medium replaced twice a week. Another group of cells was exposed to sham ultrasound as a control. Cell number, histological findings, synthesis of isomers of chondroitin sulfate, and stiffness of the chondrocyte–collagen gel composites were analyzed. US exposure promoted synthesis of chondroitin sulfate, especially chondroitin 6-sulfate, although it did not significantly enhance cell number and stiffness. In this three-dimensional culture model, these results suggest that US exposure may be clinically useful in improving the quality of chondrocyte–Atelocollagen implants for transplantation into articular cartilage defects. © 2001 Wiley Periodicals, Inc. J Biomed Mater Res 59: 201–206, 2002
Kenzo Kawasaki - One of the best experts on this subject based on the ideXlab platform.
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effects of low intensity pulsed ultrasound on proliferation and chondroitin sulfate synthesis of Cultured Chondrocytes embedded in atelocollagen gel
Journal of Biomedical Materials Research, 2002Co-Authors: T Nishikori, Kenichi Katsube, H Kataoka, Kenzo Kawasaki, Shunji Maniwa, Yuji Uchio, Mitsuo Ochi, Masakazu KuriwakaAbstract:The effects of low-intensity pulsed ultrasound (US) on the proliferation and chondroitin sulfate synthesis of Cultured Chondrocytes embedded in Atelocollagen® gel in vitro were examined. Articular cartilage was harvested from the hip, knee, and shoulder joints of 10-week-old Japanese white rabbits. Chondrocytes isolated by collagenase digestion were embedded in type I collagen gel, Atelocollagen gel, and were Cultured in Dulbecco's modified eagle's medium for 3 weeks. The US apparatus, SAFHS® , was used to deliver an ultrasound signal with spatial and temporal average intensities of 30 mW/cm2 (US group). The frequency was 1.5 MHz with a 200-microsecond tone burst repeated at 1.0 kHz. US treatments were administered for 20 min per day under culture dishes, with the medium replaced twice a week. Another group of cells was exposed to sham ultrasound as a control. Cell number, histological findings, synthesis of isomers of chondroitin sulfate, and stiffness of the chondrocyte–collagen gel composites were analyzed. US exposure promoted synthesis of chondroitin sulfate, especially chondroitin 6-sulfate, although it did not significantly enhance cell number and stiffness. In this three-dimensional culture model, these results suggest that US exposure may be clinically useful in improving the quality of chondrocyte–Atelocollagen implants for transplantation into articular cartilage defects. © 2001 Wiley Periodicals, Inc. J Biomed Mater Res 59: 201–206, 2002
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Hyaluronic acid enhances proliferation and chondroitin sulfate synthesis in Cultured Chondrocytes embedded in collagen gels.
Journal of cellular physiology, 1999Co-Authors: Kenzo Kawasaki, Yuji Uchio, Mitsuo Ochi, Nobuo Adachi, Masahiko MatsusakiAbstract:The effects of hyaluronic acid (HA) on the proliferation and chondroitin sulfate (CS) synthesis of Chondrocytes embedded in collagen gels were examined. Articular cartilage was isolated from the humerus, femur, and tibia of 21 10-week-old Japanese white rabbits. Chondrocytes isolated by collagenase digestion were embedded in type I collagen gels and Cultured in Dulbecco's modified Eagle's medium (DMEM) with various doses of HA for 4 weeks. Histological and biochemical evaluations were performed at postculture weeks 1, 2, 3, and 4. For biochemical evaluations, isomers such as chondroitin 6-sulfate (delta(di)-6S) and chondroitin 4-sulfate (delta(di)-4S) synthesized by Cultured Chondrocytes were determined by high performance liquid chromatography (HPLC) combined with fluorometry. Morphological and histological studies demonstrated that HA-treated Chondrocytes in collagen gel proliferated profusely while maintaining their phenotype. At postculture week 4, 0.1 mg/ml of HA induced an eightfold increase in cell counts compared with HA pretreatment values, or 1.5-fold more than control group. Synthesis of delta(di)-6S (delta(di)-6S content/cell) in groups treated with 0.01 and 0.1 mg/ml of HA significantly increased, while gel accumulation rates in groups treated with 0.1 and 1.0 mg/ml of HA scored significantly higher values than other groups. In collagen gel culture, HA enhanced the proliferation and delta(di)-6S synthesis of Chondrocytes while maintaining their phenotype. In clinical application, since the supply of autologous Chondrocytes for transplantation is not unlimited, the HA-treated culture method may be useful for increasing the number of Chondrocytes and thus improving the quality of implants.
Sven Kili - One of the best experts on this subject based on the ideXlab platform.
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Matrix-Applied Characterized Autologous Cultured Chondrocytes Versus Microfracture Two-Year Follow-up of a Prospective Randomized Trial
The American journal of sports medicine, 2014Co-Authors: Daniel B.f. Saris, Andrew Price, Wojciech Widuchowski, Marion Bertrand-marchand, Jacob Caron, Jon Olav Drogset, Pieter J. Emans, Ales Podskubka, A.i. Tsuchida, Sven KiliAbstract:Background:Randomized controlled trials studying the efficacy and safety of matrix-applied characterized autologous Cultured Chondrocytes (MACI) versus microfracture (MFX) for treating cartilage defects are limited.Purpose:To compare the clinical efficacy and safety of MACI versus MFX in the treatment of patients with symptomatic cartilage defects of the knee.Study Design:Randomized controlled clinical trial; Level of evidence, 1.Methods:Patients enrolled in the SUMMIT (Demonstrate the Superiority of MACI implant to Microfracture Treatment) trial had ≥1 symptomatic focal cartilage defect (Outerbridge grade III or IV; ≥3 cm2) of the femoral condyles or trochlea, with a baseline Knee Injury and Osteoarthritis Outcome Score (KOOS) pain value
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matrix applied characterized autologous Cultured Chondrocytes versus microfracture two year follow up of a prospective randomized trial
American Journal of Sports Medicine, 2014Co-Authors: Daniel B.f. Saris, Wojciech Widuchowski, Jacob Caron, Jon Olav Drogset, Pieter J. Emans, Ales Podskubka, A.i. Tsuchida, A J Price, Marion Bertrandmarchand, Sven KiliAbstract:Background:Randomized controlled trials studying the efficacy and safety of matrix-applied characterized autologous Cultured Chondrocytes (MACI) versus microfracture (MFX) for treating cartilage defects are limited.Purpose:To compare the clinical efficacy and safety of MACI versus MFX in the treatment of patients with symptomatic cartilage defects of the knee.Study Design:Randomized controlled clinical trial; Level of evidence, 1.Methods:Patients enrolled in the SUMMIT (Demonstrate the Superiority of MACI implant to Microfracture Treatment) trial had ≥1 symptomatic focal cartilage defect (Outerbridge grade III or IV; ≥3 cm2) of the femoral condyles or trochlea, with a baseline Knee Injury and Osteoarthritis Outcome Score (KOOS) pain value <55. The co–primary efficacy endpoint was the change in the KOOS pain and function subscores from baseline to 2 years. Histological evaluation and magnetic resonance imaging (MRI) assessments of structural repair tissue, treatment failure, the remaining 3 KOOS subscales,...
