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Xiaobei Zhan - One of the best experts on this subject based on the ideXlab platform.
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Effect of Curdlan on the quality of frozen-cooked noodles during frozen storage
Journal of Cereal Science, 2020Co-Authors: Ying Liang, Zhuoting Qu, Jinshui Wang, Jie Li, Xiaobei ZhanAbstract:Abstract Frozen-cooked noodles (FCN) and its components undergo quality changes during frozen storage, such as reduced textural and cooking qualities, weakened gluten network, and damaged starch properties; thus, storage condition is a critical factor affecting the final quality of FCN. In this study, in view of the thermoirreversible high-strength gel property (at ≥ 80 °C) of Curdlan, strong hydrophilicity, and freeze-thawed stability of high-strength gel prepared from Curdlan powder, the effect of Curdlan on the quality of FCN during frozen storage was first evaluated. The results showed that Curdlan was effective in reducing cooking loss, enhancing water absorption, and improving textural properties of FCN; the improving effect presented a trend of first increasing and then decreasing with the amount of increasing Curdlan (0.1%–0.9%); and the addition of 0.5% Curdlan was most effective in improving the quality of FCN. Thermal gravimetric analysis indicated that Curdlan enhanced thermal stability of FCN, implying Curdlan could strengthen the gluten network. Meanwhile, structural observations revealed that, during frozen storage, FCN with added Curdlan exhibited a more continuous and compact gluten network accompanied with more uniform and smaller ice crystals. Thus, Curdlan is desirable to be used as a novel gum in FCN to provide specific functionality and minimize the negative effect of frozen storage. This study provides new insights into the quality improvement of FCN and further expands the application potential of Curdlan in food industry.
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influence of tween 80 on the production and structure of water insoluble Curdlan from agrobacterium sp
International Journal of Biological Macromolecules, 2018Co-Authors: Ying Liang, Jianrong Wu, Zhiyong Zheng, Xiaobei ZhanAbstract:Abstract In order to explore the mechanism by which Tween-80 enhances the production of Curdlan produced by Agrobacterium sp., the effects of Tween-80 on the production and structure of Curdlan and Agrobacterium sp. were evaluated. Maximum Curdlan production (51.94 g/L) was achieved when 16 g/L Tween-80 was added at the beginning of the cell growth stage. The addition of Tween-80 at higher concentration inhibited cell growth. However, the addition of 16 g/L Tween-80 enhanced the production of Curdlan with a looser ultrastructure, significantly weakened the envelopment of Curdlan on Agrobacterium sp., altered the fine structure of cell membrane, and increased the cell membrane permeability. Moreover, the efficiency of oxygen and mass transport, respiration intensity, UTP regeneration, ATP regeneration, activity of Curdlan synthetase, capacity of stress response and energy supply of Agrobacterium sp. were all greatly improved by the addition of Tween-80. These findings demonstrate the mechanisms by which Tween-80 enhances Curdlan production and provide a cheap and feasible approach to weaken the envelopment of water-insoluble polysaccharides on bacteria.
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A new effective process for production of Curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of Curdlan particles in water suspension
Applied Microbiology and Biotechnology, 2013Co-Authors: Jing Li, Xiaobei Zhan, Zhiyong Zheng, Yu Zong, Wei-jiang LiAbstract:Biologically active β-1,3-oligosaccharides with rapidly growing biomedical applications are produced from hydrolysis of Curdlan polysaccharide. The water-insoluble Curdlan impedes its hydrolysis efficiency which is enhanced by our newly developed alkali-neutralization treatment process to increase the stability of Curdlan suspension to more than 20 days, while the untreated control settled within 5 min. A putative double-layer structure model comprising of a compact core and a hydrated outer layer was proposed to describe the treated Curdlan particles based on sedimentation and scanning electron microscopy observation. This model was verified by single- and two-step acid hydrolysis, indicative of the reduced susceptibility to hydrolysis when close to the compact core. Electrospray ionization-mass spectrometry, thin-layer chromatography analyses, and effective HPLC procedure led to the development of improved process to produce purified individual β-1,3-oligosaccharides with degrees of polymerization from 2 to 10 and potential for biomedical applications from Curdlan hydrolyzate. Our new Curdlan oligosaccharide production process offers an even better alternative to the previously published processes.
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improved Curdlan fermentation process based on optimization of dissolved oxygen combined with ph control and metabolic characterization of agrobacterium sp atcc 31749
Applied Microbiology and Biotechnology, 2012Co-Authors: Hongtao Zhang, Xiaobei Zhan, Zhiyong Zheng, Jianrong Wu, Nike English, Xiaobin YuAbstract:A significant problem in scale-down cultures, rarely studied for metabolic characterization and Curdlan-producing Agrobacterium sp. ATCC 31749, is the presence of dissolved oxygen (DO) gradients combined with pH control. Constant DO, between 5% and 75%, was maintained during batch fermentations by manipulating the agitation with PID system. Fermentation, metabolic and kinetic characterization studies were conducted in a scale-down system. The Curdlan yield, intracellular nucleotide levels and glucose conversion efficiency into Curdlan were significantly affected by DO concentrations. The optimum DO concentrations for Curdlan production were 45–60%. The average Curdlan yield, Curdlan productivity and glucose conversion efficiency into Curdlan were enhanced by 80%, 66% and 32%, respectively, compared to that at 15% DO. No apparent difference in the gel strength of the resulting Curdlan was detected. The comparison of Curdlan biosynthesis and cellular nucleotide levels showed that Curdlan production had positive relationship with intracellular levels of UTP, ADP, AMP, NAD+, NADH and UDP-glucose. The Curdlan productivity under 45% DO and 60% DO was different during 20–50 h. However, after 60 h Curdlan productivity of both conditions was similar. On that basis, a simple and reproducible two-stage DO control process for Curdlan production was developed. Curdlan production yield reached 42.8 g/l, an increase of 30% compared to that of the single agitation speed control process.
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recent advances in Curdlan biosynthesis biotechnological production and applications
Applied Microbiology and Biotechnology, 2012Co-Authors: Xiaobei Zhan, Hongtao ZhangAbstract:Curdlan is a water-insoluble β-(1,3)-glucan produced by Agrobacterium species under nitrogen-limited condition. Its heat-induced gelling properties render Curdlan to be very useful in the food industry initially. Recent advances in the understanding of the role Curdlan plays in both innate and adaptive immunity lead to its growing applications in biomedicine. Our review focuses on the recent advances on Curdlan biosynthesis and the improvements of Curdlan fermentation production both from our laboratory and many others as well as the latest advances on the new applications of Curdlan and its derivatives particularly in their immunological functions in biomedicine.
Young Hoon Park - One of the best experts on this subject based on the ideXlab platform.
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residual phosphate concentration under nitrogen limiting conditions regulates Curdlan production in agrobacterium species
Journal of Industrial Microbiology & Biotechnology, 2000Co-Authors: Young Ha Rhee, Young Hoon ParkAbstract:We investigated the influence of inorganic phosphate concentration on the production of Curdlan by Agrobacterium species. A two-step culture method was employed where cells were first cultured, followed by Curdlan production under nitrogen-limiting conditions. In the Curdlan production step, cells did not grow but metabolized sugar into Curdlan. Shake-flask experiments showed that the optimal phosphate concentration for Curdlan production was in the range of 0.1–0.3 g l−1. As the cell concentration increased from 0.42 to 1.68 g l−1 in shake-flask cultures, Curdlan production increased from 0.44 to 2.80 g l−1. However, the optimal phosphate concentration range was not dependent upon cell concentration. The specific production rate was about 70 mg Curdlan g-cell−1 h−1 irrespective of cell concentration. When the phosphate concentration was maintained at 0.5 g l−1 under nitrogen-limiting conditions, as high as 65 g l−1 of Curdlan was obtained in 120 h. Journal of Industrial Microbiology & Biotechnology (2000) 25, 180–183.
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optimal ph control of batch processes for production of Curdlan by agrobacterium species
Journal of Industrial Microbiology & Biotechnology, 1999Co-Authors: Young Hoon ParkAbstract:We sought an optimal pH profile to maximize Curdlan production in a batch fermentation of Agrobacterium species. The optimal pH profile was calculated using a gradient iteration algorithm based on the minimum principle of Pontryagin. The model equations describing cell growth and Curdlan production were developed as functions of pH, sucrose concentration, and ammonium concentration, since the specific rates of cell growth and Curdlan production were highly influenced by those parameters. The pH profile provided the strategy to shift the culture pH from the optimal growth condition (pH 7.0) to the optimal production one (pH 5.5) at the time of ammonium exhaustion. By applying the optimal pH profile in the batch process, we obtained significant improvement in Curdlan production (64 g L−1) compared to that of constant pH operation (36 g L−1).
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influence of agitation speed on production of Curdlan by agrobacterium species
Bioprocess Engineering, 1999Co-Authors: Jinkee Jung, Young Hoon ParkAbstract:Influence of dissolved oxygen level on production of Curdlan by Agrobacterium species was investigated. Preliminary shake flask experiments showed that both cell growth and Curdlan production were higher at a smaller volume of medium (50–100 ml in 500 ml flasks). As culture volume increased from 100 ml to 300 ml, both cell concentration and Curdlan production decreased, indicating that higher oxygen transfer is required for a higher production of Curdlan. Time profiles of cell concentration and Curdlan production in a 5-liter jar fermentation at different agitation speeds, ranging from 300 rpm to 700 rpm, supported the fact of higher production of Curdlan at higher oxygen transfer rate observed in shake flask cultures. At a higher agitation speed (600 rpm), the highest Curdlan production (64.4 g/l) was obtained in 120 h of a batch fermentation. However, Curdlan production was not improved at the higher agitation speed (700 rpm). For the mass production of Curdlan, fermentation was performed in a 300-liter fermenter under the condition where the same volumetric oxygen transfer coefficient was obtained as in 5-liter jar fermentation. As high as 9.28 kg of Curdlan with a final concentration of 58 g/l was obtained in 120 h batch cultivation, enlarging the potential in the industrial production of Curdlan.
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higher intracellular levels of uridinemonophosphate under nitrogen limited conditions enhance metabolic flux of Curdlan synthesis in agrobacterium species
Biotechnology and Bioengineering, 1999Co-Authors: Jeongheon Ko, Young Ha Rhee, Young Hoon ParkAbstract:Changes of intracellular nucleotide levels and their stimulatory effects on Curdlan synthesis in Agrobacterium species were investigated under different culture conditions. Under nitrogen-limited conditions where Curdlan synthesis was stimulated, intracellular levels of UMP were as high as 87 and those of AMP were 78 nmol/mg of cellular protein, while those under nitrogen-sufficient conditions were lower than 45 nmol/mg-protein. The levels of other nucleotides such as UDP, UTP, UDP-glucose, ADP, ATP, and ADP-glucose were lower than 30 nmol/mg-protein under both nitrogen-limited and sufficient conditions. The time profiles of Curdlan synthesis and cellular nucleotide levels showed that Curdlan synthesis had a positive relationship with intracellular levels of UMP and AMP. After the ammonium concentration in the medium fell below 0.1 g/L, intracellular levels of UMP and AMP increased, followed by Curdlan synthesis. However, no significant changes in the specific activities of UMP kinase, UDP kinase, and UDP-glucose pyrophosphorylase were observed during cultivation. In vitro enzyme reactions for the synthesis of UDP-glucose, which serve as a precursor for Curdlan synthesis, demonstrated that the synthesis of UDP-glucose increased with the increase of UMP concentration. In contrast, AMP had no effect on UDP-glucose synthesis at all. Addition of UMP in the medium increased the Curdlan synthesis, whereas Curdlan synthesis was inhibited in the presence of AMP. From these results, we concluded that only the higher intracellular UMP levels caused by nitrogen limitation in the medium enhance the metabolic flux of Curdlan synthesis by promoting cellular UDP-glucose synthesis. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 62: 317–323, 1999.
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Production of Curdlan using sucrose or sugar cane molasses by two-step fed-batch cultivation of Agrobacterium species
Journal of Industrial Microbiology & Biotechnology, 1997Co-Authors: Chan Soo Park, Young Hoon ParkAbstract:Maltose and sucrose were efficient carbon sources for the production of Curdlan by a strain of Agrobacterium sp. A two-step, fed-batch operation was designed in which biomass was first produced, followed by Curdlan production which was stimulated by nitrogen limitation. There exists an optimal timing for nitrogen limitation for Curdlan production in the two-step, fed-batch operation. Maximum Curdlan production (60 g L−1) was obtained from sucrose with a productivity of 0.2 g L−1 h−1 when nitrogen was limited at a cell concentration of 16.0 g L−1. It was also noted that the Curdlan yield from sucrose was as high as 0.45 g Curdlan g−1 sucrose, and the highest specific production rate was 1.0 g Curdlan g−1 cells h−1 right after nitrogen limitation. Of particular importance was the use of molasses as a cheap carbon source to produce Curdlan in the two-step, fed-batch cultivation. As high as 42 g L−1 of Curdlan with a yield of 0.35 g Curdlan g−1 total sugar was obtained after 120 h of fed-batch cultivation.
Zhiyong Zheng - One of the best experts on this subject based on the ideXlab platform.
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influence of tween 80 on the production and structure of water insoluble Curdlan from agrobacterium sp
International Journal of Biological Macromolecules, 2018Co-Authors: Ying Liang, Jianrong Wu, Zhiyong Zheng, Xiaobei ZhanAbstract:Abstract In order to explore the mechanism by which Tween-80 enhances the production of Curdlan produced by Agrobacterium sp., the effects of Tween-80 on the production and structure of Curdlan and Agrobacterium sp. were evaluated. Maximum Curdlan production (51.94 g/L) was achieved when 16 g/L Tween-80 was added at the beginning of the cell growth stage. The addition of Tween-80 at higher concentration inhibited cell growth. However, the addition of 16 g/L Tween-80 enhanced the production of Curdlan with a looser ultrastructure, significantly weakened the envelopment of Curdlan on Agrobacterium sp., altered the fine structure of cell membrane, and increased the cell membrane permeability. Moreover, the efficiency of oxygen and mass transport, respiration intensity, UTP regeneration, ATP regeneration, activity of Curdlan synthetase, capacity of stress response and energy supply of Agrobacterium sp. were all greatly improved by the addition of Tween-80. These findings demonstrate the mechanisms by which Tween-80 enhances Curdlan production and provide a cheap and feasible approach to weaken the envelopment of water-insoluble polysaccharides on bacteria.
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A new effective process for production of Curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of Curdlan particles in water suspension
Applied Microbiology and Biotechnology, 2013Co-Authors: Jing Li, Xiaobei Zhan, Zhiyong Zheng, Yu Zong, Wei-jiang LiAbstract:Biologically active β-1,3-oligosaccharides with rapidly growing biomedical applications are produced from hydrolysis of Curdlan polysaccharide. The water-insoluble Curdlan impedes its hydrolysis efficiency which is enhanced by our newly developed alkali-neutralization treatment process to increase the stability of Curdlan suspension to more than 20 days, while the untreated control settled within 5 min. A putative double-layer structure model comprising of a compact core and a hydrated outer layer was proposed to describe the treated Curdlan particles based on sedimentation and scanning electron microscopy observation. This model was verified by single- and two-step acid hydrolysis, indicative of the reduced susceptibility to hydrolysis when close to the compact core. Electrospray ionization-mass spectrometry, thin-layer chromatography analyses, and effective HPLC procedure led to the development of improved process to produce purified individual β-1,3-oligosaccharides with degrees of polymerization from 2 to 10 and potential for biomedical applications from Curdlan hydrolyzate. Our new Curdlan oligosaccharide production process offers an even better alternative to the previously published processes.
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improved Curdlan fermentation process based on optimization of dissolved oxygen combined with ph control and metabolic characterization of agrobacterium sp atcc 31749
Applied Microbiology and Biotechnology, 2012Co-Authors: Hongtao Zhang, Xiaobei Zhan, Zhiyong Zheng, Jianrong Wu, Nike English, Xiaobin YuAbstract:A significant problem in scale-down cultures, rarely studied for metabolic characterization and Curdlan-producing Agrobacterium sp. ATCC 31749, is the presence of dissolved oxygen (DO) gradients combined with pH control. Constant DO, between 5% and 75%, was maintained during batch fermentations by manipulating the agitation with PID system. Fermentation, metabolic and kinetic characterization studies were conducted in a scale-down system. The Curdlan yield, intracellular nucleotide levels and glucose conversion efficiency into Curdlan were significantly affected by DO concentrations. The optimum DO concentrations for Curdlan production were 45–60%. The average Curdlan yield, Curdlan productivity and glucose conversion efficiency into Curdlan were enhanced by 80%, 66% and 32%, respectively, compared to that at 15% DO. No apparent difference in the gel strength of the resulting Curdlan was detected. The comparison of Curdlan biosynthesis and cellular nucleotide levels showed that Curdlan production had positive relationship with intracellular levels of UTP, ADP, AMP, NAD+, NADH and UDP-glucose. The Curdlan productivity under 45% DO and 60% DO was different during 20–50 h. However, after 60 h Curdlan productivity of both conditions was similar. On that basis, a simple and reproducible two-stage DO control process for Curdlan production was developed. Curdlan production yield reached 42.8 g/l, an increase of 30% compared to that of the single agitation speed control process.
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enhanced Curdlan production in agrobacterium sp atcc 31749 by addition of low polyphosphates
Biotechnology and Bioprocess Engineering, 2011Co-Authors: Lijun Yu, Xiaobei Zhan, Jianrong Wu, Zhiyong ZhengAbstract:A large amount of adenosine triphosphate with high energy phosphate bonds is required for uridine triphosphate regeneration during Curdlan biosynthesis by Agrobacterium sp. ATCC 31749. To supply high energy for Curdlan synthesis, three low-polyphosphates (Na4P2O7, Na5P3O10, and (NaPO3)6) with higher energy phosphate bonds were employed to substitute for KH2PO4-K2HPO4 in fermentation medium. Two genes encoding the polyphosphate metabolizing enzymes, polyphosphate kinase and exopolyphosphatase, were amplified and showed 95% homology to those in Agrobacterium sp. C58 by sequence analysis. The Curdlan yields were enhanced by 23 and 134% when phosphate concentrations 0.024 mol/L of Na5P3O10 and 0.048 mol/L of (NaPO3)6 respectively, were added in the medium. The maximum Curdlan yield of 30 ± 1.02 g/L was obtained with the addition of 0.048 mol/L of (NaPO3)6 with 5 g/L CaCO3 in the medium. When CaCO3 was removed from the culture and the three lowpolyphosphates were added, the pH and biomass yield dropped remarkably and little or no Curdlan was produced. The culture containing 0.048 mol/L of (NaPO3)6 was mixed with KH2PO4-K2HPO4 and CaCO3 in the medium, but showed no effect on Curdlan production. However, Curdlan yield was improved by 49 ∼ 60% when CaCO3 was removed from the medium and KH2PO4-K2HPO4 acted as a buffer. It appears that the positive effect of (NaPO3)6 on Curdlan production required the buffering capacity of CaCO3 and the absence of KH2PO4-K2HPO4 competing as a phosphate supplier.
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New Strategy for Enhancement Curdlan Biosynthesis in Alcaligenes faecalis by Activating Gene Expression
2010 4th International Conference on Bioinformatics and Biomedical Engineering, 2010Co-Authors: Hongtao Zhang, Xiaobei Zhan, Zhiyong Zheng, Jianrong Wu, Dingqiang ChenAbstract:Curdlan (β-1, 3-glucan) is a kind of exopoly-saccharide excreted by bacteria and has the ability of improvement of immune and anticancer. It has potential values as medical material in medicine field. Many researchers began to focus on the interaction of Curdlan and immune signal protein for finding the detail mechanism of its bioactivity. The aim of this work was to investigate the effect of antibiotics and ammonia additions in combination on the enhancement of Curdlan biosynthesis. A two-stage fed-batch process was used for Curdlan production in shake flask culture of Alcaligenes faecalis. With the optimized concentration of 100mg/L of ampicillin was added to the culture medium and fermented for 5 days, it was achieved a concentration of 24.65g/L Curdlan and a yield of 10.67 Curdlan g/g cell, which were 2.02-fold and 2.1-fold of those without addition of ampicillin. With the streptomycin instead of ampicillin, a similar enhancement of Curdlan production was observed, reaching a yield of 11.78 g/g cell and a concentration of 20.77g/L Curdlan. It was demonstrated that the enhancement of Curdlan production was related to the increased activity of key enzymes involving Curdlan biosynthesis. At the same time, the cell wall biosynthesis in the bacterium was inhibited by antibiotics.
Hongtao Zhang - One of the best experts on this subject based on the ideXlab platform.
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improved Curdlan fermentation process based on optimization of dissolved oxygen combined with ph control and metabolic characterization of agrobacterium sp atcc 31749
Applied Microbiology and Biotechnology, 2012Co-Authors: Hongtao Zhang, Xiaobei Zhan, Zhiyong Zheng, Jianrong Wu, Nike English, Xiaobin YuAbstract:A significant problem in scale-down cultures, rarely studied for metabolic characterization and Curdlan-producing Agrobacterium sp. ATCC 31749, is the presence of dissolved oxygen (DO) gradients combined with pH control. Constant DO, between 5% and 75%, was maintained during batch fermentations by manipulating the agitation with PID system. Fermentation, metabolic and kinetic characterization studies were conducted in a scale-down system. The Curdlan yield, intracellular nucleotide levels and glucose conversion efficiency into Curdlan were significantly affected by DO concentrations. The optimum DO concentrations for Curdlan production were 45–60%. The average Curdlan yield, Curdlan productivity and glucose conversion efficiency into Curdlan were enhanced by 80%, 66% and 32%, respectively, compared to that at 15% DO. No apparent difference in the gel strength of the resulting Curdlan was detected. The comparison of Curdlan biosynthesis and cellular nucleotide levels showed that Curdlan production had positive relationship with intracellular levels of UTP, ADP, AMP, NAD+, NADH and UDP-glucose. The Curdlan productivity under 45% DO and 60% DO was different during 20–50 h. However, after 60 h Curdlan productivity of both conditions was similar. On that basis, a simple and reproducible two-stage DO control process for Curdlan production was developed. Curdlan production yield reached 42.8 g/l, an increase of 30% compared to that of the single agitation speed control process.
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recent advances in Curdlan biosynthesis biotechnological production and applications
Applied Microbiology and Biotechnology, 2012Co-Authors: Xiaobei Zhan, Hongtao ZhangAbstract:Curdlan is a water-insoluble β-(1,3)-glucan produced by Agrobacterium species under nitrogen-limited condition. Its heat-induced gelling properties render Curdlan to be very useful in the food industry initially. Recent advances in the understanding of the role Curdlan plays in both innate and adaptive immunity lead to its growing applications in biomedicine. Our review focuses on the recent advances on Curdlan biosynthesis and the improvements of Curdlan fermentation production both from our laboratory and many others as well as the latest advances on the new applications of Curdlan and its derivatives particularly in their immunological functions in biomedicine.
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New Strategy for Enhancement Curdlan Biosynthesis in Alcaligenes faecalis by Activating Gene Expression
2010 4th International Conference on Bioinformatics and Biomedical Engineering, 2010Co-Authors: Hongtao Zhang, Xiaobei Zhan, Zhiyong Zheng, Jianrong Wu, Dingqiang ChenAbstract:Curdlan (β-1, 3-glucan) is a kind of exopoly-saccharide excreted by bacteria and has the ability of improvement of immune and anticancer. It has potential values as medical material in medicine field. Many researchers began to focus on the interaction of Curdlan and immune signal protein for finding the detail mechanism of its bioactivity. The aim of this work was to investigate the effect of antibiotics and ammonia additions in combination on the enhancement of Curdlan biosynthesis. A two-stage fed-batch process was used for Curdlan production in shake flask culture of Alcaligenes faecalis. With the optimized concentration of 100mg/L of ampicillin was added to the culture medium and fermented for 5 days, it was achieved a concentration of 24.65g/L Curdlan and a yield of 10.67 Curdlan g/g cell, which were 2.02-fold and 2.1-fold of those without addition of ampicillin. With the streptomycin instead of ampicillin, a similar enhancement of Curdlan production was observed, reaching a yield of 11.78 g/g cell and a concentration of 20.77g/L Curdlan. It was demonstrated that the enhancement of Curdlan production was related to the increased activity of key enzymes involving Curdlan biosynthesis. At the same time, the cell wall biosynthesis in the bacterium was inhibited by antibiotics.
Rachel R Chen - One of the best experts on this subject based on the ideXlab platform.
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genome sequence of the Curdlan producing agrobacterium sp strain atcc 31749
Journal of Bacteriology, 2011Co-Authors: Anne M Ruffing, Marlene Castromelchor, Wei Shou Hu, Rachel R ChenAbstract:ABSTRACT Agrobacterium sp. ATCC 31749 is an industrial strain for the commercial production of Curdlan, an important exopolysaccharide with food and medical applications. Here we report the genome sequence of the Curdlan-producing strain ATCC 31749. Genome sequencing is the first step toward the understanding of regulation of Curdlan biosynthesis.
