The Experts below are selected from a list of 3786 Experts worldwide ranked by ideXlab platform
Yalin Tang - One of the best experts on this subject based on the ideXlab platform.
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direct detection of potassium and lead ii ions based on assembly disassembly of a chiral Cyanine Dye tba complex
Talanta, 2019Co-Authors: Gang Shen, Junfeng Xiang, Hong Zhang, F M Yang, Wei Wang, Jinghua Zhang, Tao Sun, Yalin TangAbstract:Abstract A highly selective and sensitive direct detection of potassium (K+) and lead (Pb2+) ions was developed by using the assembly and disassembly of a chiral Cyanine Dye/TBA complex. The Dye DMSB (3-ethyl-2-[3-(3-ethyl-3H-benzoselenazol-2-ylidene)-2-methylprop-1-enyl] benzoselenazolium bromide) loses the ability of self-assembly, but it can be activated by thrombin-binding aptamer (TBA) G-quadruplex structure. And only the TBA G-quadruplex formed in the presence of K+, can strongly induce J-aggregate signals of DMSB. Because the Pb2+ ions can bind and stabilize the TBA G-quadruplex with much higher efficiency than K+, the J-aggregate signals of DMSB falls sharply when the Pb2+ is present. As a result, the assembly and disassembly of DMSB allows the selective detection of 10 μM K+ and 20 nM Pb2+ respectively, even the competitive sodium ion (Na+) was as high as 145 mM. The linear correlation existed between the J-aggregate intensity and the concentration of K+ and Pb2+ over the range of 0.5–5.0 mM and 200–2000 nM, respectively. Moreover, the concentration of K+ (∼3 mM) and Pb2+ (below 20 nM) in human blood serum samples were determined by the present method, which agreed well with inductively coupled plasma mass spectrometry (ICP-MS). This work not only opens a door for the further development of G-quadruplex-based aptasensor in complex real system, but also provides a simple and versatile sensing platform for ion detection in clinic.
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a colorimetric and fluorometric dual modal dna logic gate based on the response of a Cyanine Dye supramolecule to g quadruplexes
New Journal of Chemistry, 2016Co-Authors: Hongbo Chen, Hongxia Sun, Xiufeng Zhang, Xiaoran Sun, Yunhua Shi, Yalin TangAbstract:A colorimetric and fluorometric dual-modal DNA logic gate has been constructed, which is based on the response of a Cyanine Dye supramolecule to K+–Pb2+-switched G-quadruplexes. Only when the input is K+, the output is 1, which made the system an INHIBIT logic gate. The gate operation constructed here is label-free, and is of simple design and low-cost, showing great promise for practical onsite applications.
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visual detection of mercury ii based on recognition of the g quadruplex conformational transition by a Cyanine Dye supramolecule
Analyst, 2015Co-Authors: Hongbo Chen, Hongxia Sun, Xiufeng Zhang, Xiaoran Sun, Yunhua Shi, Yalin TangAbstract:A supramolecular probe for visual detection of mercury (Hg) has been designed by using a Cyanine Dye and AS1411 G-quadruplexes, which exhibits an obvious color change from red to blue in response to an increased level of Hg(2+). The supramolecular probe exhibits high selectivity and sensitivity towards Hg(2+) and is promising for the detection of environmental samples with the naked eye.
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recognizing parallel stranded g quadruplex by Cyanine Dye dimer based on dual site binding mode
Chinese Chemical Letters, 2015Co-Authors: Wei Gai, Hongxia Sun, Qianfan Yang, Junfeng Xiang, Aijiao Guan, Lixia Wang, Yalin TangAbstract:G-quadruplexes (G4s) play important roles in biological systems, such as telomere maintenance, replication, and transcription. Based on the DNA sequence, loop geometry, and the local environments, G4s can be classified into different conformations. It is important to detect different types of G4s to monitor the diseases related with G4s. Most ligands bind to G4s based on end-stacking modes, while rare ligands bind to G4s through groove binding modes. We have found that a Cyanine Dye DMSB interacts with parallel G4 by end-stacking and groove simultaneous binding mode. In this article, we found that DMSB could simply discriminate parallel G4s from other DNA motifs by using UV-vis spectrum. These results give some clues to develop high specificity G4 probes. (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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a dual site simultaneous binding mode in the interaction between parallel stranded g quadruplex d tggggt 4 and Cyanine Dye 2 2 diethyl 9 methyl selenacarboCyanine bromide
Nucleic Acids Research, 2013Co-Authors: Wei Gai, Hongxia Sun, Qianfan Yang, Junfeng Xiang, Qian Shang, Aijiao Guan, Hong Zhang, Wei Jiang, Yalin TangAbstract:G-quadruplexes have attracted growing attention as a potential cancer-associated target for both treatment and detection in recent years. For detection purpose, high specificity is one of the most important factors to be considered in G-quadruplex probe design. It is well known that end stacking and groove binding are two dominated quadruplex-ligand binding modes, and currently most reported G-quadruplex probes are designed based on the former, which has been proven to show good selectivity between quadruplexes and non-quadruplexes. Because groove of G-quadruplex also has some unique chemical properties, it could be inferred that probes that can interact with both the groove and G-tetrad site of certain G-quadruplexes simultaneously might possess higher specificity in aspects of discriminating different quadruplexes. In this article, we report a Cyanine Dye as a potential novel probe scaffold that could occupy both the 5′-end external G-tetrad and the corresponding groove of the G-quadruplex simultaneously. By using various spectrum and nuclear magnetic resonance techniques, we give a detailed binding characterization for this dual-site simultaneous binding mode. A preliminary result suggests that this mode might provide highly specific recognition to a parallel-stranded G-quadruplex. These findings and the structural elucidation might give some clues in aspects of developing highly specific G-quadruplex probes.
Qianfan Yang - One of the best experts on this subject based on the ideXlab platform.
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recognizing parallel stranded g quadruplex by Cyanine Dye dimer based on dual site binding mode
Chinese Chemical Letters, 2015Co-Authors: Wei Gai, Hongxia Sun, Qianfan Yang, Junfeng Xiang, Aijiao Guan, Lixia Wang, Yalin TangAbstract:G-quadruplexes (G4s) play important roles in biological systems, such as telomere maintenance, replication, and transcription. Based on the DNA sequence, loop geometry, and the local environments, G4s can be classified into different conformations. It is important to detect different types of G4s to monitor the diseases related with G4s. Most ligands bind to G4s based on end-stacking modes, while rare ligands bind to G4s through groove binding modes. We have found that a Cyanine Dye DMSB interacts with parallel G4 by end-stacking and groove simultaneous binding mode. In this article, we found that DMSB could simply discriminate parallel G4s from other DNA motifs by using UV-vis spectrum. These results give some clues to develop high specificity G4 probes. (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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a dual site simultaneous binding mode in the interaction between parallel stranded g quadruplex d tggggt 4 and Cyanine Dye 2 2 diethyl 9 methyl selenacarboCyanine bromide
Nucleic Acids Research, 2013Co-Authors: Wei Gai, Hongxia Sun, Qianfan Yang, Junfeng Xiang, Qian Shang, Aijiao Guan, Hong Zhang, Wei Jiang, Yalin TangAbstract:G-quadruplexes have attracted growing attention as a potential cancer-associated target for both treatment and detection in recent years. For detection purpose, high specificity is one of the most important factors to be considered in G-quadruplex probe design. It is well known that end stacking and groove binding are two dominated quadruplex-ligand binding modes, and currently most reported G-quadruplex probes are designed based on the former, which has been proven to show good selectivity between quadruplexes and non-quadruplexes. Because groove of G-quadruplex also has some unique chemical properties, it could be inferred that probes that can interact with both the groove and G-tetrad site of certain G-quadruplexes simultaneously might possess higher specificity in aspects of discriminating different quadruplexes. In this article, we report a Cyanine Dye as a potential novel probe scaffold that could occupy both the 5′-end external G-tetrad and the corresponding groove of the G-quadruplex simultaneously. By using various spectrum and nuclear magnetic resonance techniques, we give a detailed binding characterization for this dual-site simultaneous binding mode. A preliminary result suggests that this mode might provide highly specific recognition to a parallel-stranded G-quadruplex. These findings and the structural elucidation might give some clues in aspects of developing highly specific G-quadruplex probes.
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visual detection of potassium by a Cyanine Dye supramolecular aggregate responsive to g quadruplex motif transition
Analyst, 2012Co-Authors: Hongxia Sun, Qianfan Yang, Junfeng Xiang, Wei Gai, Qian Shang, Aijiao Guan, Yan Liu, Yalin TangAbstract:A supramolecular probe is designed for visual detection of potassium based on a novel Cyanine Dye aggregate recognizing the motif transition of telomeric G-quadruplexes under the Na+ background. The practical application for colorimetric measurement of urine potassium has been tested.
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verification of intramolecular hybrid parallel g quadruplex structure under physiological conditions using novel Cyanine Dye h aggregates both in solution and on au film
Analytical Chemistry, 2010Co-Authors: Qianfan Yang, Xiufeng Zhang, Junfeng Xiang, Aijiao Guan, Shu Yang, Qiuju Zhou, Yanxia Zhang, Hong Zhang, Yalin TangAbstract:The H-aggregates of a novel Cyanine Dye, 3,3′-di(3-sulfopropyl)-4,5,4′,5′-dibenzo-9-methyl-thiacarboCyanine triethylammonium salt (MTC), have been fabricated to verify hybrid/parallel intramolecular G-quadruplexes from linear duplex and single-strand DNAs under physiological conditions. The recognition is found to be successful both in solution and on Au film. These results have shown MTC H-aggregates, as a supramolecular system, may be used as a potential excellent probe for DNA structure, both in vitro and in vivo.
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verification of specific g quadruplex structure by using a novel Cyanine Dye supramolecular assembly ii the binding characterization with specific intramolecular g quadruplex and the recognizing mechanism
Nucleic Acids Research, 2010Co-Authors: Qianfan Yang, Xiufeng Zhang, Junfeng Xiang, Aijiao Guan, Shu Yang, Qiuju Zhou, Hong Zhang, Yalin TangAbstract:The supramolecular assembly of a novel Cyanine Dye, 3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-ethyl-thiacarboCyanine triethylammonium salt (ETC) was designed to verify specific intramolecular G-quadruplexes from duplex and single-strand DNAs. Spectral results have shown that ETC presented two major distinct signatures with specific intramolecular G-quadruplexes in vitro: (i) dramatic changes in the absorption spectra (including disappearance of absorption peak around 660 nm and appearance of independent new peak around 584 nm); (ii) approximately 70 times enhancement of fluorescence signal at 600 nm. Furthermore, based on (1)H-nuclear magnetic resonance and circular dichroism results, the preferring binding of ETC to specific intramolecular G-quadruplexes probably result from end-stacking, and the loop structure nearby also plays an important role.
Junfeng Xiang - One of the best experts on this subject based on the ideXlab platform.
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direct detection of potassium and lead ii ions based on assembly disassembly of a chiral Cyanine Dye tba complex
Talanta, 2019Co-Authors: Gang Shen, Junfeng Xiang, Hong Zhang, F M Yang, Wei Wang, Jinghua Zhang, Tao Sun, Yalin TangAbstract:Abstract A highly selective and sensitive direct detection of potassium (K+) and lead (Pb2+) ions was developed by using the assembly and disassembly of a chiral Cyanine Dye/TBA complex. The Dye DMSB (3-ethyl-2-[3-(3-ethyl-3H-benzoselenazol-2-ylidene)-2-methylprop-1-enyl] benzoselenazolium bromide) loses the ability of self-assembly, but it can be activated by thrombin-binding aptamer (TBA) G-quadruplex structure. And only the TBA G-quadruplex formed in the presence of K+, can strongly induce J-aggregate signals of DMSB. Because the Pb2+ ions can bind and stabilize the TBA G-quadruplex with much higher efficiency than K+, the J-aggregate signals of DMSB falls sharply when the Pb2+ is present. As a result, the assembly and disassembly of DMSB allows the selective detection of 10 μM K+ and 20 nM Pb2+ respectively, even the competitive sodium ion (Na+) was as high as 145 mM. The linear correlation existed between the J-aggregate intensity and the concentration of K+ and Pb2+ over the range of 0.5–5.0 mM and 200–2000 nM, respectively. Moreover, the concentration of K+ (∼3 mM) and Pb2+ (below 20 nM) in human blood serum samples were determined by the present method, which agreed well with inductively coupled plasma mass spectrometry (ICP-MS). This work not only opens a door for the further development of G-quadruplex-based aptasensor in complex real system, but also provides a simple and versatile sensing platform for ion detection in clinic.
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recognizing parallel stranded g quadruplex by Cyanine Dye dimer based on dual site binding mode
Chinese Chemical Letters, 2015Co-Authors: Wei Gai, Hongxia Sun, Qianfan Yang, Junfeng Xiang, Aijiao Guan, Lixia Wang, Yalin TangAbstract:G-quadruplexes (G4s) play important roles in biological systems, such as telomere maintenance, replication, and transcription. Based on the DNA sequence, loop geometry, and the local environments, G4s can be classified into different conformations. It is important to detect different types of G4s to monitor the diseases related with G4s. Most ligands bind to G4s based on end-stacking modes, while rare ligands bind to G4s through groove binding modes. We have found that a Cyanine Dye DMSB interacts with parallel G4 by end-stacking and groove simultaneous binding mode. In this article, we found that DMSB could simply discriminate parallel G4s from other DNA motifs by using UV-vis spectrum. These results give some clues to develop high specificity G4 probes. (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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a dual site simultaneous binding mode in the interaction between parallel stranded g quadruplex d tggggt 4 and Cyanine Dye 2 2 diethyl 9 methyl selenacarboCyanine bromide
Nucleic Acids Research, 2013Co-Authors: Wei Gai, Hongxia Sun, Qianfan Yang, Junfeng Xiang, Qian Shang, Aijiao Guan, Hong Zhang, Wei Jiang, Yalin TangAbstract:G-quadruplexes have attracted growing attention as a potential cancer-associated target for both treatment and detection in recent years. For detection purpose, high specificity is one of the most important factors to be considered in G-quadruplex probe design. It is well known that end stacking and groove binding are two dominated quadruplex-ligand binding modes, and currently most reported G-quadruplex probes are designed based on the former, which has been proven to show good selectivity between quadruplexes and non-quadruplexes. Because groove of G-quadruplex also has some unique chemical properties, it could be inferred that probes that can interact with both the groove and G-tetrad site of certain G-quadruplexes simultaneously might possess higher specificity in aspects of discriminating different quadruplexes. In this article, we report a Cyanine Dye as a potential novel probe scaffold that could occupy both the 5′-end external G-tetrad and the corresponding groove of the G-quadruplex simultaneously. By using various spectrum and nuclear magnetic resonance techniques, we give a detailed binding characterization for this dual-site simultaneous binding mode. A preliminary result suggests that this mode might provide highly specific recognition to a parallel-stranded G-quadruplex. These findings and the structural elucidation might give some clues in aspects of developing highly specific G-quadruplex probes.
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visual detection of potassium by a Cyanine Dye supramolecular aggregate responsive to g quadruplex motif transition
Analyst, 2012Co-Authors: Hongxia Sun, Qianfan Yang, Junfeng Xiang, Wei Gai, Qian Shang, Aijiao Guan, Yan Liu, Yalin TangAbstract:A supramolecular probe is designed for visual detection of potassium based on a novel Cyanine Dye aggregate recognizing the motif transition of telomeric G-quadruplexes under the Na+ background. The practical application for colorimetric measurement of urine potassium has been tested.
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verification of intramolecular hybrid parallel g quadruplex structure under physiological conditions using novel Cyanine Dye h aggregates both in solution and on au film
Analytical Chemistry, 2010Co-Authors: Qianfan Yang, Xiufeng Zhang, Junfeng Xiang, Aijiao Guan, Shu Yang, Qiuju Zhou, Yanxia Zhang, Hong Zhang, Yalin TangAbstract:The H-aggregates of a novel Cyanine Dye, 3,3′-di(3-sulfopropyl)-4,5,4′,5′-dibenzo-9-methyl-thiacarboCyanine triethylammonium salt (MTC), have been fabricated to verify hybrid/parallel intramolecular G-quadruplexes from linear duplex and single-strand DNAs under physiological conditions. The recognition is found to be successful both in solution and on Au film. These results have shown MTC H-aggregates, as a supramolecular system, may be used as a potential excellent probe for DNA structure, both in vitro and in vivo.
Harry L. Anderson - One of the best experts on this subject based on the ideXlab platform.
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A Complex Comprising a Cyanine Dye Rotaxane and a Porphyrin Nanoring as a Model Light‐Harvesting System
Angewandte Chemie (International ed. in English), 2020Co-Authors: Jiratheep Pruchyathamkorn, William J. Kendrick, Andrew T. Frawley, Andrea Mattioni, Felipe Caycedo-soler, Susana F. Huelga, Martin B. Plenio, Harry L. AndersonAbstract:A nanoring-rotaxane supramolecular assembly with a Cy7 Cyanine Dye (hexamethylindotricarboCyanine) threaded along the axis of the nanoring was synthesized as a model for the energy transfer between the light-harvesting complex LH1 and the reaction center in purple bacteria photosynthesis. The complex displays efficient energy transfer from the central Cyanine Dye to the surrounding zinc porphyrin nanoring. We present a theoretical model that reproduces the absorption spectrum of the nanoring and quantifies the excitonic coupling between the nanoring and the central Dye, thereby explaining the efficient energy transfer and demonstrating similarity with structurally related natural light-harvesting systems.
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a complex comprising a Cyanine Dye rotaxane and a porphyrin nanoring as a model light harvesting system
Angewandte Chemie, 2020Co-Authors: Jiratheep Pruchyathamkorn, William J. Kendrick, Andrew T. Frawley, Andrea Mattioni, Susana F. Huelga, Martin B. Plenio, Felipe Caycedosoler, Harry L. AndersonAbstract:A nanoring-rotaxane supramolecular assembly with a Cy7 Cyanine Dye (hexamethylindotricarboCyanine) threaded along the axis of the nanoring was synthesized as a model for the energy transfer between the light-harvesting complex LH1 and the reaction center in purple bacteria photosynthesis. The complex displays efficient energy transfer from the central Cyanine Dye to the surrounding zinc porphyrin nanoring. We present a theoretical model that reproduces the absorption spectrum of the nanoring and quantifies the excitonic coupling between the nanoring and the central Dye, thereby explaining the efficient energy transfer and demonstrating similarity with structurally related natural light-harvesting systems.
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rotaxane encapsulated Cyanine Dyes enhanced fluorescence efficiency and photostability
Chemical Communications, 2000Co-Authors: Jonathan E H Buston, James R Young, Harry L. AndersonAbstract:Two isomers of a Cyanine Dye α-cyclodextrin rotaxane have been synthesised in aqueous solution, and structurally characterised by 2D NMR spectroscopy; they exhibit enhanced fluorescence and photostability, compared with the free Dye.
Hongxia Sun - One of the best experts on this subject based on the ideXlab platform.
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a colorimetric and fluorometric dual modal dna logic gate based on the response of a Cyanine Dye supramolecule to g quadruplexes
New Journal of Chemistry, 2016Co-Authors: Hongbo Chen, Hongxia Sun, Xiufeng Zhang, Xiaoran Sun, Yunhua Shi, Yalin TangAbstract:A colorimetric and fluorometric dual-modal DNA logic gate has been constructed, which is based on the response of a Cyanine Dye supramolecule to K+–Pb2+-switched G-quadruplexes. Only when the input is K+, the output is 1, which made the system an INHIBIT logic gate. The gate operation constructed here is label-free, and is of simple design and low-cost, showing great promise for practical onsite applications.
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visual detection of mercury ii based on recognition of the g quadruplex conformational transition by a Cyanine Dye supramolecule
Analyst, 2015Co-Authors: Hongbo Chen, Hongxia Sun, Xiufeng Zhang, Xiaoran Sun, Yunhua Shi, Yalin TangAbstract:A supramolecular probe for visual detection of mercury (Hg) has been designed by using a Cyanine Dye and AS1411 G-quadruplexes, which exhibits an obvious color change from red to blue in response to an increased level of Hg(2+). The supramolecular probe exhibits high selectivity and sensitivity towards Hg(2+) and is promising for the detection of environmental samples with the naked eye.
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recognizing parallel stranded g quadruplex by Cyanine Dye dimer based on dual site binding mode
Chinese Chemical Letters, 2015Co-Authors: Wei Gai, Hongxia Sun, Qianfan Yang, Junfeng Xiang, Aijiao Guan, Lixia Wang, Yalin TangAbstract:G-quadruplexes (G4s) play important roles in biological systems, such as telomere maintenance, replication, and transcription. Based on the DNA sequence, loop geometry, and the local environments, G4s can be classified into different conformations. It is important to detect different types of G4s to monitor the diseases related with G4s. Most ligands bind to G4s based on end-stacking modes, while rare ligands bind to G4s through groove binding modes. We have found that a Cyanine Dye DMSB interacts with parallel G4 by end-stacking and groove simultaneous binding mode. In this article, we found that DMSB could simply discriminate parallel G4s from other DNA motifs by using UV-vis spectrum. These results give some clues to develop high specificity G4 probes. (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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a dual site simultaneous binding mode in the interaction between parallel stranded g quadruplex d tggggt 4 and Cyanine Dye 2 2 diethyl 9 methyl selenacarboCyanine bromide
Nucleic Acids Research, 2013Co-Authors: Wei Gai, Hongxia Sun, Qianfan Yang, Junfeng Xiang, Qian Shang, Aijiao Guan, Hong Zhang, Wei Jiang, Yalin TangAbstract:G-quadruplexes have attracted growing attention as a potential cancer-associated target for both treatment and detection in recent years. For detection purpose, high specificity is one of the most important factors to be considered in G-quadruplex probe design. It is well known that end stacking and groove binding are two dominated quadruplex-ligand binding modes, and currently most reported G-quadruplex probes are designed based on the former, which has been proven to show good selectivity between quadruplexes and non-quadruplexes. Because groove of G-quadruplex also has some unique chemical properties, it could be inferred that probes that can interact with both the groove and G-tetrad site of certain G-quadruplexes simultaneously might possess higher specificity in aspects of discriminating different quadruplexes. In this article, we report a Cyanine Dye as a potential novel probe scaffold that could occupy both the 5′-end external G-tetrad and the corresponding groove of the G-quadruplex simultaneously. By using various spectrum and nuclear magnetic resonance techniques, we give a detailed binding characterization for this dual-site simultaneous binding mode. A preliminary result suggests that this mode might provide highly specific recognition to a parallel-stranded G-quadruplex. These findings and the structural elucidation might give some clues in aspects of developing highly specific G-quadruplex probes.
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visual detection of potassium by a Cyanine Dye supramolecular aggregate responsive to g quadruplex motif transition
Analyst, 2012Co-Authors: Hongxia Sun, Qianfan Yang, Junfeng Xiang, Wei Gai, Qian Shang, Aijiao Guan, Yan Liu, Yalin TangAbstract:A supramolecular probe is designed for visual detection of potassium based on a novel Cyanine Dye aggregate recognizing the motif transition of telomeric G-quadruplexes under the Na+ background. The practical application for colorimetric measurement of urine potassium has been tested.
