The Experts below are selected from a list of 195 Experts worldwide ranked by ideXlab platform
Arvind Varsani - One of the best experts on this subject based on the ideXlab platform.
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Single stranded DNA viruses associated with Capybara Faeces sampled in Brazil.
Viruses, 2019Co-Authors: Rafaela S. Fontenele, Arvind Varsani, Kara Schmidlin, Cristiano Lacorte, N. S. Lamas, Simone G. RibeiroAbstract:Capybaras (Hydrochoerus hydrochaeris), the world’s largest rodents, are distributed throughout South America. These wild herbivores are commonly found near water bodies and are well adapted to rural and urban areas. There is limited information on the viruses circulating through capybaras. This study aimed to expand the knowledge on the viral diversity associated with capybaras by sampling their faeces. Using a viral metagenomics approach, we identified diverse single-stranded DNA viruses in the capybara faeces sampled in the Distrito Federal, Brazil. A total of 148 complete genomes of viruses in the Microviridae family were identified. In addition, 14 genomoviruses (family Genomoviridae), a novel Cyclovirus (family Circoviridae), and a smacovirus (family Smacoviridae) were identified. Also, 37 diverse viruses that cannot be assigned to known families and more broadly referred to as unclassified circular replication associated protein encoding single-stranded (CRESS) DNA viruses were identified. This study provides a snapshot of the viral diversity associated with capybaras that may be infectious to these animals or associated with their microbiota or diet.
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ICTV Virus Taxonomy Profile: Circoviridae.
Journal of General Virology, 2017Co-Authors: Mya Breitbart, Karyna Rosario, Eric Delwart, Joaquim Segalés, Arvind VarsaniAbstract:The family Circoviridae comprises viruses with small, circular, single-stranded DNA (ssDNA) genomes, including the smallest known animal viruses. Members of this family are classified into two genera, Circovirus and Cyclovirus, which are distinguished by the position of the origin of replication relative to the coding regions and the length of the intergenic regions. Within each genus, the species demarcation threshold is 80 % genome-wide nucleotide sequence identity. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Circoviridae, which is available at www.ictv.global/report/circoviridae.
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Revisiting the Taxonomy of the Family Circoviridae: Establishment of the Genus Cyclovirus and Removal of the Genus Gyrovirus
Archives of Virology, 2017Co-Authors: Karyna Rosario, Mya Breitbart, Eric Delwart, Joaquim Segalés, Balázs Harrach, Philippe Biagini, Arvind VarsaniAbstract:The family Circoviridae contains viruses with covalently closed, circular, single-stranded DNA (ssDNA) genomes, including the smallest known autonomously replicating, capsid-encoding animal pathogens. Members of this family are known to cause fatal diseases in birds and pigs and have been historically classified in one of two genera: Circovirus, which contains avian and porcine pathogens, and Gyrovirus, which includes a single species (Chicken anemia virus). However, over the course of the past six years, viral metagenomic approaches as well as degenerate PCR detection in unconventional hosts and environmental samples have elucidated a broader host range, including fish, a diversity of mammals, and invertebrates, for members of the family Circoviridae. Notably, these methods have uncovered a distinct group of viruses that are closely related to members of the genus Circovirus and comprise a new genus, Cyclovirus. The discovery of new viruses and a re-evaluation of genomic features that characterize members of the Circoviridae prompted a revision of the classification criteria used for this family of animal viruses. Here we provide details on an updated Circoviridae taxonomy ratified by the International Committee on the Taxonomy of Viruses in 2016, which establishes the genus Cyclovirus and reassigns the genus Gyrovirus to the family Anelloviridae, a separate lineage of animal viruses that also contains circular ssDNA genomes. In addition, we provide a new species demarcation threshold of 80% genome-wide pairwise identity for members of the family Circoviridae, based on pairwise identity distribution analysis, and list guidelines to distinguish between members of this family and other eukaryotic viruses with circular, ssDNA genomes.
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High Global Diversity of Cycloviruses Amongst Dragonflies
Journal of General Virology, 2013Co-Authors: Anisha Dayaram, Milen Marinov, Karyna Rosario, Mya Breitbart, Kristen A. Potter, Angela B. Moline, Dana Drake Rosenstein, John E. Thomas, Gerardo R. Argüello-astorga, Arvind VarsaniAbstract:Members of the family Circoviridae, specifically the genus Circovirus, were thought to infect only vertebrates; however, members of a sister group under the same family, the proposed genusCyclovirus, have been detected recently in insects. In an effort to explore the diversity of Cycloviruses and better understand the evolution of these novel ssDNA viruses, here we present five Cycloviruses isolated from three dragonfly species (Orthetrum sabina, Xanthocnemis zealandica and Rhionaeschna multicolor) collected in Australia, New Zealand and the USA, respectively. The genomes of these five viruses share similar genome structure to other Cycloviruses, with a circular ~1.7 kb genome and two major bidirectionally transcribed ORFs. The genomic sequence data gathered during this study were combined with all Cyclovirus genomes available in public databases to identify conserved motifs and regulatory elements in the intergenic regions, as well as determine diversity and recombinant regions within their genomes. The genomes reported here represent four different Cyclovirus species, three of which are novel. Our results confirm that Cycloviruses circulate widely in winged-insect populations; in eight different Cyclovirus species identified in dragonflies to date, some of these exhibit a broad geographical distribution. Recombination analysis revealed both intra-and inter-species recombination events amongst Cycloviruses, including genomes recovered from disparate sources (e.g. goat meat and human faeces). Similar to other well-characterized circular ssDNA viruses, recombination may play an important role in Cyclovirus evolution. © 2013 SGM.
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Dragonfly Cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)
Journal of General Virology, 2011Co-Authors: Karyna Rosario, Elizabeth J. Wiltshire, Daisy Stainton, Simona Kraberger, Milen Marinov, Matthew Walters, Douglas A. Collings, Darren P. Martin, Mya Breitbart, Arvind VarsaniAbstract:Dragonfly Cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (familyLibellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share >95 % nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48–63 % genome-wide nucleotide identity with Cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in Cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.
Eric Delwart - One of the best experts on this subject based on the ideXlab platform.
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ICTV Virus Taxonomy Profile: Circoviridae.
Journal of General Virology, 2017Co-Authors: Mya Breitbart, Karyna Rosario, Eric Delwart, Joaquim Segalés, Arvind VarsaniAbstract:The family Circoviridae comprises viruses with small, circular, single-stranded DNA (ssDNA) genomes, including the smallest known animal viruses. Members of this family are classified into two genera, Circovirus and Cyclovirus, which are distinguished by the position of the origin of replication relative to the coding regions and the length of the intergenic regions. Within each genus, the species demarcation threshold is 80 % genome-wide nucleotide sequence identity. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Circoviridae, which is available at www.ictv.global/report/circoviridae.
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Revisiting the Taxonomy of the Family Circoviridae: Establishment of the Genus Cyclovirus and Removal of the Genus Gyrovirus
Archives of Virology, 2017Co-Authors: Karyna Rosario, Mya Breitbart, Eric Delwart, Joaquim Segalés, Balázs Harrach, Philippe Biagini, Arvind VarsaniAbstract:The family Circoviridae contains viruses with covalently closed, circular, single-stranded DNA (ssDNA) genomes, including the smallest known autonomously replicating, capsid-encoding animal pathogens. Members of this family are known to cause fatal diseases in birds and pigs and have been historically classified in one of two genera: Circovirus, which contains avian and porcine pathogens, and Gyrovirus, which includes a single species (Chicken anemia virus). However, over the course of the past six years, viral metagenomic approaches as well as degenerate PCR detection in unconventional hosts and environmental samples have elucidated a broader host range, including fish, a diversity of mammals, and invertebrates, for members of the family Circoviridae. Notably, these methods have uncovered a distinct group of viruses that are closely related to members of the genus Circovirus and comprise a new genus, Cyclovirus. The discovery of new viruses and a re-evaluation of genomic features that characterize members of the Circoviridae prompted a revision of the classification criteria used for this family of animal viruses. Here we provide details on an updated Circoviridae taxonomy ratified by the International Committee on the Taxonomy of Viruses in 2016, which establishes the genus Cyclovirus and reassigns the genus Gyrovirus to the family Anelloviridae, a separate lineage of animal viruses that also contains circular ssDNA genomes. In addition, we provide a new species demarcation threshold of 80% genome-wide pairwise identity for members of the family Circoviridae, based on pairwise identity distribution analysis, and list guidelines to distinguish between members of this family and other eukaryotic viruses with circular, ssDNA genomes.
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Genomic characterization of novel circular ssDNA viruses from insectivorous bats in Southern Brazil.
PLOS ONE, 2015Co-Authors: Francisco Esmaile De Sales Lima, Eric Delwart, Samuel Paulo Cibulski, Helton Fernandes Dos Santos, Thais Fumaco Teixeira, Ana Paula Muterle Varela, Paulo Michel Roehe, Ana Cláudia FrancoAbstract:Circoviruses are highly prevalent porcine and avian pathogens. In recent years, novel circular ssDNA genomes have recently been detected in a variety of fecal and environmental samples using deep sequencing approaches. In this study the identification of genomes of novel circoviruses and Cycloviruses in feces of insectivorous bats is reported. Pan-reactive primers were used targeting the conserved rep region of circoviruses and Cycloviruses to screen DNA bat fecal samples. Using this approach, partial rep sequences were detected which formed five phylogenetic groups distributed among the Circovirus and the recently proposed Cyclovirus genera of the Circoviridae. Further analysis using inverse PCR and Sanger sequencing led to the characterization of four new putative members of the family Circoviridae with genome size ranging from 1,608 to 1,790 nt, two inversely arranged ORFs, and canonical nonamer sequences atop a stem loop.
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Faecal virome of cats in an animal shelter.
The Journal of general virology, 2014Co-Authors: Wen Zhang, Beatrix Kapusinszky, Patricia A Pesavento, Linlin Li, Xutao Deng, Eric DelwartAbstract:We describe the metagenomics-derived feline enteric virome in the faeces of 25 cats from a single shelter in California. More than 90 % of the recognizable viral reads were related to mammalian viruses and the rest to bacterial viruses. Eight viral families were detected: Astroviridae, Coronaviridae, Parvoviridae, Circoviridae, Herpesviridae, Anelloviridae, Caliciviridae and Picobirnaviridae. Six previously known viruses were also identified: feline coronavirus type 1, felid herpes 1, feline calicivirus, feline norovirus, feline panleukopenia virus and picobirnavirus. Novel species of astroviruses and bocaviruses, and the first genome of a Cyclovirus in a feline were characterized. The RNA-dependent RNA polymerase region from four highly divergent partial viral genomes in the order Picornavirales were sequenced. The detection of such a diverse collection of viruses shed within a single shelter suggested that such animals experience robust viral exposures. This study increases our understanding of the viral diversity in cats, facilitating future evaluation of their pathogenic and zoonotic potentials.
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Cyclovirus in nasopharyngeal aspirates of chilean children with respiratory infections
Journal of General Virology, 2014Co-Authors: Xutao Deng, Eric Delwart, Tung Gia Phan, Vivian Luchsinger, Luis F AvendanoAbstract:Some respiratory tract infections remain unexplained despite extensive testing for common pathogens. Nasopharyngeal aspirates (NPAs) from 120 Chilean infants from Santiago with acute lower respiratory tract infections were analysed by viral metagenomics, revealing the presence of nucleic acids from anelloviruses, adenovirus-associated virus and 12 known respiratory viral pathogens. A single sequence read showed translated protein similarity to Cycloviruses. We used inverse PCR to amplify the complete circular ssDNA genome of a novel Cyclovirus we named CyCV-ChileNPA1. Closely related variants were detected using PCR in the NPAs of three other affected children that also contained anelloviruses. This report increases the current knowledge of the genetic diversity of Cycloviruses whose detection in multiple NPAs may reflect a tropism for human respiratory tissues.
Karyna Rosario - One of the best experts on this subject based on the ideXlab platform.
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ICTV Virus Taxonomy Profile: Circoviridae.
Journal of General Virology, 2017Co-Authors: Mya Breitbart, Karyna Rosario, Eric Delwart, Joaquim Segalés, Arvind VarsaniAbstract:The family Circoviridae comprises viruses with small, circular, single-stranded DNA (ssDNA) genomes, including the smallest known animal viruses. Members of this family are classified into two genera, Circovirus and Cyclovirus, which are distinguished by the position of the origin of replication relative to the coding regions and the length of the intergenic regions. Within each genus, the species demarcation threshold is 80 % genome-wide nucleotide sequence identity. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Circoviridae, which is available at www.ictv.global/report/circoviridae.
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Revisiting the Taxonomy of the Family Circoviridae: Establishment of the Genus Cyclovirus and Removal of the Genus Gyrovirus
Archives of Virology, 2017Co-Authors: Karyna Rosario, Mya Breitbart, Eric Delwart, Joaquim Segalés, Balázs Harrach, Philippe Biagini, Arvind VarsaniAbstract:The family Circoviridae contains viruses with covalently closed, circular, single-stranded DNA (ssDNA) genomes, including the smallest known autonomously replicating, capsid-encoding animal pathogens. Members of this family are known to cause fatal diseases in birds and pigs and have been historically classified in one of two genera: Circovirus, which contains avian and porcine pathogens, and Gyrovirus, which includes a single species (Chicken anemia virus). However, over the course of the past six years, viral metagenomic approaches as well as degenerate PCR detection in unconventional hosts and environmental samples have elucidated a broader host range, including fish, a diversity of mammals, and invertebrates, for members of the family Circoviridae. Notably, these methods have uncovered a distinct group of viruses that are closely related to members of the genus Circovirus and comprise a new genus, Cyclovirus. The discovery of new viruses and a re-evaluation of genomic features that characterize members of the Circoviridae prompted a revision of the classification criteria used for this family of animal viruses. Here we provide details on an updated Circoviridae taxonomy ratified by the International Committee on the Taxonomy of Viruses in 2016, which establishes the genus Cyclovirus and reassigns the genus Gyrovirus to the family Anelloviridae, a separate lineage of animal viruses that also contains circular ssDNA genomes. In addition, we provide a new species demarcation threshold of 80% genome-wide pairwise identity for members of the family Circoviridae, based on pairwise identity distribution analysis, and list guidelines to distinguish between members of this family and other eukaryotic viruses with circular, ssDNA genomes.
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High Global Diversity of Cycloviruses Amongst Dragonflies
Journal of General Virology, 2013Co-Authors: Anisha Dayaram, Milen Marinov, Karyna Rosario, Mya Breitbart, Kristen A. Potter, Angela B. Moline, Dana Drake Rosenstein, John E. Thomas, Gerardo R. Argüello-astorga, Arvind VarsaniAbstract:Members of the family Circoviridae, specifically the genus Circovirus, were thought to infect only vertebrates; however, members of a sister group under the same family, the proposed genusCyclovirus, have been detected recently in insects. In an effort to explore the diversity of Cycloviruses and better understand the evolution of these novel ssDNA viruses, here we present five Cycloviruses isolated from three dragonfly species (Orthetrum sabina, Xanthocnemis zealandica and Rhionaeschna multicolor) collected in Australia, New Zealand and the USA, respectively. The genomes of these five viruses share similar genome structure to other Cycloviruses, with a circular ~1.7 kb genome and two major bidirectionally transcribed ORFs. The genomic sequence data gathered during this study were combined with all Cyclovirus genomes available in public databases to identify conserved motifs and regulatory elements in the intergenic regions, as well as determine diversity and recombinant regions within their genomes. The genomes reported here represent four different Cyclovirus species, three of which are novel. Our results confirm that Cycloviruses circulate widely in winged-insect populations; in eight different Cyclovirus species identified in dragonflies to date, some of these exhibit a broad geographical distribution. Recombination analysis revealed both intra-and inter-species recombination events amongst Cycloviruses, including genomes recovered from disparate sources (e.g. goat meat and human faeces). Similar to other well-characterized circular ssDNA viruses, recombination may play an important role in Cyclovirus evolution. © 2013 SGM.
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Novel Cyclovirus discovered in the Florida woods cockroach Eurycotis floridana (Walker).
Archives of Virology, 2013Co-Authors: Marco Padilla-rodriguez, Karyna Rosario, Mya BreitbartAbstract:A novel Cyclovirus (proposed genus “Cyclovirus”, family Circoviridae) was discovered in a specimen of Eurycotis floridana (Walker), also known as the Florida woods cockroach or palmetto bug, collected from Tarpon Springs, Florida. The Florida woods cockroach–associated Cyclovirus GS140 (FWCasCyV-GS140) was obtained through a degenerate PCR assay and showed 64 % genome-wide pairwise identity to a Cyclovirus identified in bat feces. This finding supports recent reports suggesting that Circoviridae members, traditionally thought to only infect vertebrates, are present within insect populations.
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Dragonfly Cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)
Journal of General Virology, 2011Co-Authors: Karyna Rosario, Elizabeth J. Wiltshire, Daisy Stainton, Simona Kraberger, Milen Marinov, Matthew Walters, Douglas A. Collings, Darren P. Martin, Mya Breitbart, Arvind VarsaniAbstract:Dragonfly Cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (familyLibellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share >95 % nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48–63 % genome-wide nucleotide identity with Cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in Cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.
Mya Breitbart - One of the best experts on this subject based on the ideXlab platform.
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ICTV Virus Taxonomy Profile: Circoviridae.
Journal of General Virology, 2017Co-Authors: Mya Breitbart, Karyna Rosario, Eric Delwart, Joaquim Segalés, Arvind VarsaniAbstract:The family Circoviridae comprises viruses with small, circular, single-stranded DNA (ssDNA) genomes, including the smallest known animal viruses. Members of this family are classified into two genera, Circovirus and Cyclovirus, which are distinguished by the position of the origin of replication relative to the coding regions and the length of the intergenic regions. Within each genus, the species demarcation threshold is 80 % genome-wide nucleotide sequence identity. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Circoviridae, which is available at www.ictv.global/report/circoviridae.
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Revisiting the Taxonomy of the Family Circoviridae: Establishment of the Genus Cyclovirus and Removal of the Genus Gyrovirus
Archives of Virology, 2017Co-Authors: Karyna Rosario, Mya Breitbart, Eric Delwart, Joaquim Segalés, Balázs Harrach, Philippe Biagini, Arvind VarsaniAbstract:The family Circoviridae contains viruses with covalently closed, circular, single-stranded DNA (ssDNA) genomes, including the smallest known autonomously replicating, capsid-encoding animal pathogens. Members of this family are known to cause fatal diseases in birds and pigs and have been historically classified in one of two genera: Circovirus, which contains avian and porcine pathogens, and Gyrovirus, which includes a single species (Chicken anemia virus). However, over the course of the past six years, viral metagenomic approaches as well as degenerate PCR detection in unconventional hosts and environmental samples have elucidated a broader host range, including fish, a diversity of mammals, and invertebrates, for members of the family Circoviridae. Notably, these methods have uncovered a distinct group of viruses that are closely related to members of the genus Circovirus and comprise a new genus, Cyclovirus. The discovery of new viruses and a re-evaluation of genomic features that characterize members of the Circoviridae prompted a revision of the classification criteria used for this family of animal viruses. Here we provide details on an updated Circoviridae taxonomy ratified by the International Committee on the Taxonomy of Viruses in 2016, which establishes the genus Cyclovirus and reassigns the genus Gyrovirus to the family Anelloviridae, a separate lineage of animal viruses that also contains circular ssDNA genomes. In addition, we provide a new species demarcation threshold of 80% genome-wide pairwise identity for members of the family Circoviridae, based on pairwise identity distribution analysis, and list guidelines to distinguish between members of this family and other eukaryotic viruses with circular, ssDNA genomes.
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High Global Diversity of Cycloviruses Amongst Dragonflies
Journal of General Virology, 2013Co-Authors: Anisha Dayaram, Milen Marinov, Karyna Rosario, Mya Breitbart, Kristen A. Potter, Angela B. Moline, Dana Drake Rosenstein, John E. Thomas, Gerardo R. Argüello-astorga, Arvind VarsaniAbstract:Members of the family Circoviridae, specifically the genus Circovirus, were thought to infect only vertebrates; however, members of a sister group under the same family, the proposed genusCyclovirus, have been detected recently in insects. In an effort to explore the diversity of Cycloviruses and better understand the evolution of these novel ssDNA viruses, here we present five Cycloviruses isolated from three dragonfly species (Orthetrum sabina, Xanthocnemis zealandica and Rhionaeschna multicolor) collected in Australia, New Zealand and the USA, respectively. The genomes of these five viruses share similar genome structure to other Cycloviruses, with a circular ~1.7 kb genome and two major bidirectionally transcribed ORFs. The genomic sequence data gathered during this study were combined with all Cyclovirus genomes available in public databases to identify conserved motifs and regulatory elements in the intergenic regions, as well as determine diversity and recombinant regions within their genomes. The genomes reported here represent four different Cyclovirus species, three of which are novel. Our results confirm that Cycloviruses circulate widely in winged-insect populations; in eight different Cyclovirus species identified in dragonflies to date, some of these exhibit a broad geographical distribution. Recombination analysis revealed both intra-and inter-species recombination events amongst Cycloviruses, including genomes recovered from disparate sources (e.g. goat meat and human faeces). Similar to other well-characterized circular ssDNA viruses, recombination may play an important role in Cyclovirus evolution. © 2013 SGM.
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Novel Cyclovirus discovered in the Florida woods cockroach Eurycotis floridana (Walker).
Archives of Virology, 2013Co-Authors: Marco Padilla-rodriguez, Karyna Rosario, Mya BreitbartAbstract:A novel Cyclovirus (proposed genus “Cyclovirus”, family Circoviridae) was discovered in a specimen of Eurycotis floridana (Walker), also known as the Florida woods cockroach or palmetto bug, collected from Tarpon Springs, Florida. The Florida woods cockroach–associated Cyclovirus GS140 (FWCasCyV-GS140) was obtained through a degenerate PCR assay and showed 64 % genome-wide pairwise identity to a Cyclovirus identified in bat feces. This finding supports recent reports suggesting that Circoviridae members, traditionally thought to only infect vertebrates, are present within insect populations.
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Dragonfly Cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera)
Journal of General Virology, 2011Co-Authors: Karyna Rosario, Elizabeth J. Wiltshire, Daisy Stainton, Simona Kraberger, Milen Marinov, Matthew Walters, Douglas A. Collings, Darren P. Martin, Mya Breitbart, Arvind VarsaniAbstract:Dragonfly Cyclovirus (DfCyV), a new species of ssDNA virus discovered using viral metagenomics in dragonflies (familyLibellulidae) from the Kingdom of Tonga. Metagenomic sequences of DfCyV were similar to viruses of the recently proposed genus Cyclovirus within the family Circoviridae. Specific PCRs resulted in the recovery of 21 DfCyV genomes from three dragonfly species (Pantala flavescens, Tholymis tillarga and Diplacodes bipunctata). The 1741 nt DfCyV genomes share >95 % nucleotide identity and are classified into 11 subtypes representing a single strain. The DfCyV genomes share 48–63 % genome-wide nucleotide identity with Cycloviruses identified in human faecal samples. Recombination analysis revealed three recombinant DfCyV genomes, suggesting that recombination plays an important role in Cyclovirus evolution. To our knowledge, this is the first report of a circular ssDNA virus identified in insects, and the data may help elucidate evolutionary links among novel Circoviridae recently identified in animals and environmental samples.
Tsutomu Hohdatsu - One of the best experts on this subject based on the ideXlab platform.
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Novel single-stranded, circular DNA virus identified in cats in Japan.
Archives of Virology, 2018Co-Authors: Tomomi Takano, Yoshitomo Yanai, Kanae Hiramatsu, Tomoyoshi Doki, Tsutomu HohdatsuAbstract:We detected a novel feline stool-associated circular DNA virus (FeSCV) in fecal samples from cats with diarrhea using consensus primers matching those of circovirus and Cyclovirus. FeSCV is a circular DNA virus containing a genome with a total length of 2,046 nt encoding 2 open reading frames. Phylogenetic analyses indicated that FeSCV is classified into a clade different from that of circovirus and Cyclovirus. Since the FeSCVs detected in several cats in the same household had genetically similar genomes, these viruses are most likely derived from the same origin.
