The Experts below are selected from a list of 198 Experts worldwide ranked by ideXlab platform
Anwar Farhood - One of the best experts on this subject based on the ideXlab platform.
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thyroid transcription factor 1 immunocytochemical staining of pleural fluid Cytocentrifuge preparations for detection of small cell lung carcinoma
Acta Cytologica, 2004Co-Authors: Anwar FarhoodAbstract:OBJECTIVE: To assess whether thyroid transcription factor-1(TTF-1) immunostaining of Cytocentrifuge slides previously stained by the Papanicolaou technique is feasible and useful in diagnosing small cell lung carcinoma (SCLC) in pleural fluid. STUDY DESIGN: Eleven Papanicoalou-stained Cytocentrifuge preparations of pleural fluid from 11 patients with SCLC were reviewed. The initial cytologic diagnoses were 5 positive, 1 suspicious and 5 negative for SCLC. After transferring the cytologic material to a positively charged slide, immunostaining was performed using mouse monoclonal antibody against TTF-1. RESULTS: The neoplastic cellularity varied among the single suspicious and 5 positive cases. The case with a suspicious diagnosis had scant tumor cells. All 6 cases showed nuclear staining for TTF-1 in most of the tumor cells. The other 5 cases with a negative cytologic diagnosis were negative for TTF-1. CONCLUSION: The results of this study indicate that immunostaining for TTF-1 can be successfully applied to previously Pap-stained Cytocentrifuge preparations to assist in the diagnosis of SCLC in pleural fluid.
Lance R Peterson - One of the best experts on this subject based on the ideXlab platform.
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evaluation of the Cytocentrifuge gram stain as a screening test for bacteriuria in specimens from specific patient populations
American Journal of Clinical Pathology, 1997Co-Authors: A G Winquist, Mary Ann Orrico, Lance R PetersonAbstract:: To assess the usefulness of the Cytocentrifuge Gram stain as a urine screening test in the clinical microbiology laboratory for the elimination of culture for screen-negative specimens, we compared the results of the Cytocentrifuge Gram stain to the results of culture for 1,171 urine specimens. The data were analyzed separately for specimens from males (inpatients) and females (inpatients and outpatients), as well as for catheterized and voided specimens. Overall, the Cytocentrifuge Gram stain had excellent negative predictive value (97.7%) and sensitivity (92.3%) at a culture threshold of 10(5) colony-forming units per milliliter or more. The negative predictive value and sensitivity decreased at lower culture thresholds in all populations. The negative predictive value decreased most markedly for female outpatients. Because of low positive predictive value and specificity, this test is not reliable as a sole indicator for presumptive therapy in many cases with positive results. If its limitations are recognized, the Cytocentrifuge Gram stain is a useful screening test for the rapid exclusion of bacteriuria.
J Denton - One of the best experts on this subject based on the ideXlab platform.
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Cytocentrifuge preparations 1 organisms
1991Co-Authors: A J Freemont, J DentonAbstract:The organisms most commonly identified within joints are bacteria, notably staphylococci, streptococci, Gram-negative bacilli and acid-fast bacilli. Les commonly, fungi and viruses may be encountered1.
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Cytocentrifuge preparations 5 other cells
1991Co-Authors: A J Freemont, J DentonAbstract:In addition to granular and large mononuclear cells, there are many other cell types present within synovial fluid. These tend to be either well-recognized cells of the host defence system (e.g. lymphocytes and plasma cells) or cells with a distinctive morphology, many of which are indistinguishable from cells described only in the peripheral blood in patients with haematological or systemic disorders (e.g. Reider cells, LE cells).
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Cytocentrifuge preparations 3 granular cells
1991Co-Authors: A J Freemont, J DentonAbstract:In the context of synovial fluid cytoanalysis, there are 3 main types of granular cells: 1. Neutrophil polymorphs (polymorphs) 2. Eosinophils 3. Mast cells.
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Cytocentrifuge preparations 2 synovial fluid cells
1991Co-Authors: A J Freemont, J DentonAbstract:A review of the literature would show, rightly, that neutrophils, lymphocytes and ‘mononuclear cells’ are the most commonly encountered cells in synovial fluid1–4. Unfortunately, all too frequently, it would appear from published descriptions of synovial fluid cytoanalysis that they are the only cells within synovial fluid. There is a limit to the permutations possible with three cell types; it is scarcely surprising, therefore, that it is frequently concluded that synovial fluid cytoanalysis is of only limited diagnostic value. However, it is our experience that careful examination of Cytocentrifuge preparations confirm the prediction (see Chapter 2) that it is possible to recognize a greater diversity of cell types than has been previously recorded. To fully appreciate this diversity, it is necessary to examine synovial fluid using a variety of techniques. Electron microscopy, immunocytochemistry and enzyme histochemistry reveal subgroups of cells that cannot be recognized morphologically. Unfortunately, these techniques cannot be employed as standard in most laboratories, and, although they will be used to supplement the data on the different cell types described in the next few chapters, much of the information presented will be derived from examination of cell morphology in standard Jenner—Giemsa-stained Cytocentrifuge preparations.
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Cytocentrifuge preparations 4 large mononuclear cells
1991Co-Authors: A J Freemont, J DentonAbstract:This group incorporates most of the mononuclear cells described in the literature1 and specifically excludes small lymphocytes (Chapter 13). There are four morphologically distinct subgroups within this category: 1. Monocytoid mononuclear cells (macrophages), 2. Cytophagocytic mononuclear cells (CPM), 3. Round nuclear mononuclear cells (synoviocytes), 4. Immunoblasts.
A G Winquist - One of the best experts on this subject based on the ideXlab platform.
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evaluation of the Cytocentrifuge gram stain as a screening test for bacteriuria in specimens from specific patient populations
American Journal of Clinical Pathology, 1997Co-Authors: A G Winquist, Mary Ann Orrico, Lance R PetersonAbstract:: To assess the usefulness of the Cytocentrifuge Gram stain as a urine screening test in the clinical microbiology laboratory for the elimination of culture for screen-negative specimens, we compared the results of the Cytocentrifuge Gram stain to the results of culture for 1,171 urine specimens. The data were analyzed separately for specimens from males (inpatients) and females (inpatients and outpatients), as well as for catheterized and voided specimens. Overall, the Cytocentrifuge Gram stain had excellent negative predictive value (97.7%) and sensitivity (92.3%) at a culture threshold of 10(5) colony-forming units per milliliter or more. The negative predictive value and sensitivity decreased at lower culture thresholds in all populations. The negative predictive value decreased most markedly for female outpatients. Because of low positive predictive value and specificity, this test is not reliable as a sole indicator for presumptive therapy in many cases with positive results. If its limitations are recognized, the Cytocentrifuge Gram stain is a useful screening test for the rapid exclusion of bacteriuria.
A L Hasto - One of the best experts on this subject based on the ideXlab platform.
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large volume Cytocentrifuge for processing alcohol fixed cytologic specimens application in urinary cytology
Acta Cytologica, 1991Co-Authors: T Toivonen, A L HastoAbstract:: The use of a large-volume Cytocentrifuge for the routine processing of urine specimens was investigated. Most specimens arrived in the laboratory mixed with 50% ethanol. Polyethylene glycol was added to the fixative supplied to the clinicians or to the cell suspension received in the laboratory. The slides used in the Cytocentrifuge were coated with gelatin or poly-L-lysine to minimize cell loss. The resulting monolayers of cells, with occasional true tissue fragments, were stained by the Papanicolaou method. When indicated, immunocytochemical and histochemical stains were used. The technique gave good morphologic details, good cell yield and consistent staining quality. This method has also been applied to other cytologic specimens, such as serous fluids and fine needle aspirates, with good results. The method saves the time of cytotechnologists, and slides prepared by this technique are suitable for automatic staining.
