The Experts below are selected from a list of 151374 Experts worldwide ranked by ideXlab platform
Dirk Baumjohann - One of the best experts on this subject based on the ideXlab platform.
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a microrna upregulated in asthma airway t cells promotes th2 Cytokine Production
Nature Immunology, 2014Co-Authors: Laura J Simpson, Sana Patel, Nirav R Bhakta, David F Choy, Hans Brightbill, Xin Ren, Yanli Wang, Heather H Pua, Dirk BaumjohannAbstract:miRNAs 'tune' gene expression to orchestrate cell activity. Ansel and colleagues show that miR-19 modulates TH2 Cytokine Production by targeting TH2-specific as well as general T cell–activation pathways.
Donna M. Murasko - One of the best experts on this subject based on the ideXlab platform.
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Age-Related Changes in Type 1 and Type 2 Cytokine Production in Humans
Biogerontology, 2002Co-Authors: Elizabeth M. Gardner, Donna M. MuraskoAbstract:Although aging is accompanied by several changes in immune function, altered T cell function represents the most consistent and dramatic change. Since Cytokines modulate the immune response, it has been postulated that these age-associated changes in T cell function may be due to alterations in Cytokine Production. Data from murine studies generally support an age-related shift from a Th1-like (IL-2, IFN-γ) to a Th2-like (IL-4, IL-6,IL-10) Cytokine response; however, whether or not such an age-related shift to a Type 2 Cytokine response occurs in humans is not certain. This review of over 60 studies in humans, in which Type 1 and Type 2 Cytokines were evaluated either alone or together, suggests that age-associated changes in Cytokine Production are inconsistent. Further, these age-associated changes in Cytokine Production do not always induce a shift to a Type 2 Cytokine response. Importantly, these studies highlight that the stimulus used to induce Cytokines influences both the level and pattern of immune response. Additional comprehensive evaluations in human studies are both critical and necessary to clearly identify the impact of altered Cytokine Production on age-related changes in immune function.
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Effect of age on Cytokine Production in humans
AGE, 1998Co-Authors: Erica D. Bernstein, Donna M. MuraskoAbstract:Aging is accompanied by many changes in immune response, with the most consistent and dramatic alterations occurring within the T cell compartment. Since Cytokines are central to immune cell communications, age-associated changes in Cytokine Production may contribute to these alterations. While data from murine studies suggest a switch from a Th1 (IL-2, IFNγ) to a Th2 (IL-4, IL-6, IL-10) Cytokine response, this model has not been as clearly established in humans. In addition, this current review of over 50 studies in humans suggests that age-associated changes in Cytokine Production are not consistent.
Maria G. Van Pampus - One of the best experts on this subject based on the ideXlab platform.
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Porphyromonas Gingivalis and E-coli induce different Cytokine Production patterns in pregnant women
PloS one, 2014Co-Authors: Marijke M. Faas, Alina Kunnen, Daphne C. Dekker, Hermie J. M. Harmsen, Jan G. Aarnoudse, Frank Abbas, Paul De Vos, Maria G. Van PampusAbstract:OBJECTIVE: Pregnant individuals of many species, including humans, are more sensitive to various bacteria or their products as compared with non-pregnant individuals. Pregnant individuals also respond differently to different bacteria or their products. Therefore, in the present study, we evaluated whether the increased sensitivity of pregnant women to bacterial products and their heterogeneous response to different bacteria was associated with differences in whole blood Cytokine Production upon stimulation with bacteria or their products. METHODS: Blood samples were taken from healthy pregnant and age-matched non-pregnant women and ex vivo stimulated with bacteria or LPS from Porphyromonas Gingivalis (Pg) or E-coli for 24 hrs. TNFα, IL-1s, IL-6, IL-12 and IL-10 were measured using a multiplex Luminex system. RESULTS: We observed a generally lower Cytokine Production after stimulation with Pg bacteria or it's LPS as compared with E-coli bacteria. However, there was also an effect of pregnancy upon Cytokine Production: in pregnant women the Production of IL-6 upon Pg stimulation was decreased as compared with non-pregnant women. After stimulation with E-coli, the Production of IL-12 and TNFα was decreased in pregnant women as compared with non-pregnant women. CONCLUSION: Our results showed that Cytokine Production upon bacterial stimulation of whole blood differed between pregnant and non-pregnant women, showing that the increased sensitivity of pregnant women may be due to differences in Cytokine Production. Moreover, pregnancy also affected whole blood Cytokine Production upon Pg or E-coli stimulation differently. Thus, the different responses of pregnant women to different bacteria or their products may result from variations in Cytokine Production.
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Cytokine Production induced by non-encapsulated and encapsulated Porphyromonas gingivalis strains
Archives of oral biology, 2012Co-Authors: Alina Kunnen, Daphne C. Dekker, Maria G. Van Pampus, Hermie J. M. Harmsen, Jan G. Aarnoudse, Frank Abbas, Marijke M. FaasAbstract:Abstract Objective Although the exact reason is not known, encapsulated gram-negative Porphyromonas gingivalis strains are more virulent than non-encapsulated strains. Since difference in virulence properties may be due to difference in Cytokine Production following recognition of the bacteria or their products by the host inflammatory cells, we compared Cytokine Production following stimulation with bacteria or lipopolysaccharides (LPS) of a non-encapsulated and an encapsulated P. gingivalis strain (K − and K1). Design Tumour necrosis factor-alpha (TNF-α) Production following stimulation of the cell-line Mono Mac 6 with bacteria or LPS of both P. gingivalis strains was determined using flow cytometry. Furthermore, we investigated the effects of the two P. gingivalis strains or their LPS on TNF-α and Interleukin (IL-1β, IL-6, IL-12 and IL-10) Production in whole blood using Luminex. In both experiments, Escherichia coli bacteria and LPS were used as a reference. Results Both P. gingivalis strains induced lower Cytokine Production than E. coli with the exception of IL-6. P. gingivalis K1 bacteria elicited a higher overall Cytokine Production than P. gingivalis K − . In contrast, P. gingivalis K1 LPS stimulation induced a lower Cytokine Production than P. gingivalis K − LPS. Conclusions Our findings suggest that the encapsulated P. gingivalis K1 bacteria induce higher Cytokine Production than the non-encapsulated P. gingivalis K − . This was not due to its LPS. The stronger induction of Cytokines may contribute to the higher virulence of P. gingivalis K1.
Marijke M. Faas - One of the best experts on this subject based on the ideXlab platform.
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Porphyromonas Gingivalis and E-coli induce different Cytokine Production patterns in pregnant women
PloS one, 2014Co-Authors: Marijke M. Faas, Alina Kunnen, Daphne C. Dekker, Hermie J. M. Harmsen, Jan G. Aarnoudse, Frank Abbas, Paul De Vos, Maria G. Van PampusAbstract:OBJECTIVE: Pregnant individuals of many species, including humans, are more sensitive to various bacteria or their products as compared with non-pregnant individuals. Pregnant individuals also respond differently to different bacteria or their products. Therefore, in the present study, we evaluated whether the increased sensitivity of pregnant women to bacterial products and their heterogeneous response to different bacteria was associated with differences in whole blood Cytokine Production upon stimulation with bacteria or their products. METHODS: Blood samples were taken from healthy pregnant and age-matched non-pregnant women and ex vivo stimulated with bacteria or LPS from Porphyromonas Gingivalis (Pg) or E-coli for 24 hrs. TNFα, IL-1s, IL-6, IL-12 and IL-10 were measured using a multiplex Luminex system. RESULTS: We observed a generally lower Cytokine Production after stimulation with Pg bacteria or it's LPS as compared with E-coli bacteria. However, there was also an effect of pregnancy upon Cytokine Production: in pregnant women the Production of IL-6 upon Pg stimulation was decreased as compared with non-pregnant women. After stimulation with E-coli, the Production of IL-12 and TNFα was decreased in pregnant women as compared with non-pregnant women. CONCLUSION: Our results showed that Cytokine Production upon bacterial stimulation of whole blood differed between pregnant and non-pregnant women, showing that the increased sensitivity of pregnant women may be due to differences in Cytokine Production. Moreover, pregnancy also affected whole blood Cytokine Production upon Pg or E-coli stimulation differently. Thus, the different responses of pregnant women to different bacteria or their products may result from variations in Cytokine Production.
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Cytokine Production induced by non-encapsulated and encapsulated Porphyromonas gingivalis strains
Archives of oral biology, 2012Co-Authors: Alina Kunnen, Daphne C. Dekker, Maria G. Van Pampus, Hermie J. M. Harmsen, Jan G. Aarnoudse, Frank Abbas, Marijke M. FaasAbstract:Abstract Objective Although the exact reason is not known, encapsulated gram-negative Porphyromonas gingivalis strains are more virulent than non-encapsulated strains. Since difference in virulence properties may be due to difference in Cytokine Production following recognition of the bacteria or their products by the host inflammatory cells, we compared Cytokine Production following stimulation with bacteria or lipopolysaccharides (LPS) of a non-encapsulated and an encapsulated P. gingivalis strain (K − and K1). Design Tumour necrosis factor-alpha (TNF-α) Production following stimulation of the cell-line Mono Mac 6 with bacteria or LPS of both P. gingivalis strains was determined using flow cytometry. Furthermore, we investigated the effects of the two P. gingivalis strains or their LPS on TNF-α and Interleukin (IL-1β, IL-6, IL-12 and IL-10) Production in whole blood using Luminex. In both experiments, Escherichia coli bacteria and LPS were used as a reference. Results Both P. gingivalis strains induced lower Cytokine Production than E. coli with the exception of IL-6. P. gingivalis K1 bacteria elicited a higher overall Cytokine Production than P. gingivalis K − . In contrast, P. gingivalis K1 LPS stimulation induced a lower Cytokine Production than P. gingivalis K − LPS. Conclusions Our findings suggest that the encapsulated P. gingivalis K1 bacteria induce higher Cytokine Production than the non-encapsulated P. gingivalis K − . This was not due to its LPS. The stronger induction of Cytokines may contribute to the higher virulence of P. gingivalis K1.
Fred Finkelman - One of the best experts on this subject based on the ideXlab platform.
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The in vivo Cytokine capture assay for measurement of Cytokine Production in the mouse.
Current protocols in immunology, 2003Co-Authors: Fred Finkelman, Suzanne Morris, Tatyana Orekhova, David SehyAbstract:Because most Cytokines are utilized, catabolized, or excreted shortly after they are produced, it has been difficult to directly measure in vivo Cytokine Production. Consequently, it has been necessary to infer in vivo Cytokine secretion levels from the results of ex vivo assays of Cytokine secretion, assays that measure tissue levels of Cytokine mRNA, or assays that stain tissues for Cytokine protein levels. Results of these assays provide important and useful information, but do not necessarily reflect in vivo Cytokine secretion. To better determine in vivo Cytokine Production, the in vivo Cytokine capture assay (IVCCA) was developed. IVCCA facilitates measurement of Cytokines in serum by increasing their in vivo half-lives. This increases the sensitivity of measurement of in vivo Cytokine Production 30- to 1,000-fold. The first protocol described in this unit is for luminescence-based ELISA, while the second is for an absorbance-based method.
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Development of an assay to measure in vivo Cytokine Production in the mouse
International immunology, 1999Co-Authors: Fred Finkelman, Suzanne C. MorrisAbstract:The short in vivo lifespan of many Cytokines can make measurement of in vivo Cytokine Production difficult. A method was developed to measure in vivo IL-4 and IFN-gamma Production that eliminates this problem. Mice are injected with a biotin-labeled neutralizing IgG anti-IL-4 or anti-IFN-gamma mAb and bled 2-24 h later. Secreted Cytokine is captured by the biotin-labeled mAb to produce a complex that has a relatively long in vivo half-life and consequently accumulates in serum. Serum concentrations of the complex are determined by ELISA, using wells coated with an antibody to a second epitope on the same Cytokine to capture the complex. This technique is specific and increases sensitivity of detection of secreted IL-4 at least 1000-fold. The amount of Cytokine measured is directly proportional to the amount produced and relatively independent of the site of Cytokine Production. Furthermore, because mice are injected with small quantities of biotin-labeled anti-Cytokine mAb, which sample, rather than neutralize, all secreted Cytokines, Cytokine-dependent responses are not inhibited. The in vivo half-lives of the Cytokine-anti-Cytokine mAb complexes are sufficiently short to allow Cytokine Production to be measured every 2-3 days in the same mice. Thus, use of this assay provides a practical and relatively simple and inexpensive way to measure ongoing in vivo Cytokine Production. Furthermore, the techniques that have been developed to measure in vivo Production of IL-4 and IFN-gamma can be applied to in vivo measurement of other molecules that have a short in vivo lifespan, including other Cytokines.
