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Sören Schröder - One of the best experts on this subject based on the ideXlab platform.
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Prognostic significance of nuclear DNA content and proliferative activity in renal cell carcinomas: A clinicopathologic study of 58 patients using mitotic count, MIB‐1 staining, and DNA Cytophotometry
Cancer, 1996Co-Authors: Wolfram Jochum, Sören Schröder, Ramin Al-taha, Christian August, Andreas J. Gross, Jürgen Berger, Barbara-christina PadbergAbstract:BACKGROUND For a variety of human malignancies, static DNA Cytophotometry and immunostaining for the Ki-67 antigen using the antibody MIB-1 have provided significant prognostic information. METHODS Surgical specimens of 58 renal cell carcinomas (RCCs) were investigated by conventional histology, DNA Cytophotometry, and MIB-1 immunostaining. RESULTS The MIB-1 indices and DNA data were found not only to be significantly correlated with various other morphologic parameters, but also to the clinical behavior of RCC. In the course of this study (median observation period: 31 months), 27% of patients died from RCC. None of these patients belonged to the group of 37 patients with RCCs exhibiting diploid or euploid DNA histograms. Lethal outcome occurred in only 16 of the 21 patients (76%) with noneuploid or aneuploid histogram tumors (P < 0.0001). According to their MIB-1 indices and upon choosing different cutoff levels, the 58 RCCs were categorized into 2 groups with either low or high proliferative activity. Using the median and the mean MIB-1 index as cutoffs, none of the patients with tumors showing low proliferative activity had died, whereas 16 of 29 patients (55%) or, respectively, 16 of 25 patients (64%) with tumors exhibiting high proliferative activity, had died from RCC (P < 0.0001). CONCLUSIONS In addition to tumor grade and stage, both a high MIB-1 index and a noneuploid or aneuploid DNA histogram of a given RCC have the potential to identify tumor patients with an impaired prognosis. Cancer 1996;77:514-21.
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DNA Cytophotometry and prognosis in typical and atypical bronchopulmonary carcinoids. A clinicomorphologic study of 100 neuroendocrine lung tumors.
The American journal of surgical pathology, 1996Co-Authors: Barbara-christina Padberg, Joachim Woenckhaus, Gregor Hilger, Lothar Beccu, Wolfram Jochum, Ursula Range, Hartwig Kastendieck, Sören SchröderAbstract:Surgical material obtained from 100 patients with typical carcinoids (TC) and atypical carcinoids (AC) of the lung (including 100 primary, four residual tumors, and four lymph node or distant metastases) was investigated by conventional histology and scanning DNA Cytophotometry. Of the 60 TC (96%), 58 exhibited euploid DNA histograms compared with only 20 (50%) of the 40 AC. The morphologic findings were related to the patients' survival (median observation period, 9 years). Statistical analyses disclosed the histologic type of disease (TC versus AC) and the DNA content of tumors (euploid versus aneuploid) to affect prognosis significantly (p < 0.001). Deaths resulting from tumor were exclusively observed among patients with atypical (eight of 40) or DNA aneuploid carcinoids (eight of 22). Six patients were alive with persistent tumor manifestations 3 to 20 years after initial diagnosis, four with DNA diploid primary carcinoids. The presence of lymph node metastases alone was not associated with poor prognosis as long as the primary tumor or the related metastases showed a diploid DNA content. DNA Cytophotometry thus might be regarded as an adjunctive prognostic criterion in individual carcinoid cases.
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DNA Cytophotometry and prognosis in ovarian tumors of borderline malignancy. A clinicomorphologic study of 80 cases
Cancer, 1992Co-Authors: Barbara-christina Padberg, Hartmut Arps, Sören Schröder, Ursula Franke, Carsten Thiedemann, Wolfgang Rehpenning, Hans-egon Stegner, Helmut Lietz, Manfred DietelAbstract:Surgical specimens of 80 ovarian tumors of borderline malignancy (OTBM) were investigated by scanning DNA Cytophotometry. Diploid or euploid DNA histograms were found for 21 tumors, whereas 59 OTBM showed noneuploid or aneuploid DNA patterns. All patients were followed-up after surgery for at least 3 years (mean observation period, 6.7 years). Follow-up showed 11 cases of recurrent disease and 6 deaths. DNA findings and several other morphologic and clinical details (including patient age, histologic type and stage of disease, and extent of therapy) were correlated to the postoperative course. Statistical analyses disclosed that, of these parameters, only DNA content significantly affected prognosis. Recurrences and deaths resulting from tumor exclusively were observed among patients with noneuploid or aneuploid OTBM, whereas none of the diploid or euploid tumors recurred (P less than 0.05). DNA Cytophotometry thus might be regarded as an effective complementary means to assess the prognosis of individual OTBM cases.
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DNA Cytophotometry in adrenocortical tumours: A clinicomorphological study of 66 cases
Virchows Archiv A, 1991Co-Authors: Barbara-christina Padberg, Ines Lauritzen, Eike Achilles, Konstanze Holl, Max Bressel, Günter Klöppel, Henning Dralle, Sören SchröderAbstract:Surgical specimens of 66 adrenocortical tumours were investigated by conventional microscopy and DNA Cytophotometry. Histologically, 50 neoplasms were classified as adenomas and 16 as carcinomas. In only 8 of the latter cases were distant metastases and/or a lethal outcome recorded. On single cell scanning Cytophotometry either non-euploid or aneuploid DNA histograms were identified in 24 of 50 adenomas (48%) and in 14 of 16 carcinomas (88%). The two carcinomas exhibiting euploid DNA distributions fell into the group of 7 malignancies which are recurrence-free so far. From these findings it is concluded that DNA measurements have no diagnostic and only limited prognostic value in neoplasms of the adrenal cortex.
Barbara-christina Padberg - One of the best experts on this subject based on the ideXlab platform.
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Prognostic significance of nuclear DNA content and proliferative activity in renal cell carcinomas: A clinicopathologic study of 58 patients using mitotic count, MIB‐1 staining, and DNA Cytophotometry
Cancer, 1996Co-Authors: Wolfram Jochum, Sören Schröder, Ramin Al-taha, Christian August, Andreas J. Gross, Jürgen Berger, Barbara-christina PadbergAbstract:BACKGROUND For a variety of human malignancies, static DNA Cytophotometry and immunostaining for the Ki-67 antigen using the antibody MIB-1 have provided significant prognostic information. METHODS Surgical specimens of 58 renal cell carcinomas (RCCs) were investigated by conventional histology, DNA Cytophotometry, and MIB-1 immunostaining. RESULTS The MIB-1 indices and DNA data were found not only to be significantly correlated with various other morphologic parameters, but also to the clinical behavior of RCC. In the course of this study (median observation period: 31 months), 27% of patients died from RCC. None of these patients belonged to the group of 37 patients with RCCs exhibiting diploid or euploid DNA histograms. Lethal outcome occurred in only 16 of the 21 patients (76%) with noneuploid or aneuploid histogram tumors (P < 0.0001). According to their MIB-1 indices and upon choosing different cutoff levels, the 58 RCCs were categorized into 2 groups with either low or high proliferative activity. Using the median and the mean MIB-1 index as cutoffs, none of the patients with tumors showing low proliferative activity had died, whereas 16 of 29 patients (55%) or, respectively, 16 of 25 patients (64%) with tumors exhibiting high proliferative activity, had died from RCC (P < 0.0001). CONCLUSIONS In addition to tumor grade and stage, both a high MIB-1 index and a noneuploid or aneuploid DNA histogram of a given RCC have the potential to identify tumor patients with an impaired prognosis. Cancer 1996;77:514-21.
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DNA Cytophotometry and prognosis in typical and atypical bronchopulmonary carcinoids. A clinicomorphologic study of 100 neuroendocrine lung tumors.
The American journal of surgical pathology, 1996Co-Authors: Barbara-christina Padberg, Joachim Woenckhaus, Gregor Hilger, Lothar Beccu, Wolfram Jochum, Ursula Range, Hartwig Kastendieck, Sören SchröderAbstract:Surgical material obtained from 100 patients with typical carcinoids (TC) and atypical carcinoids (AC) of the lung (including 100 primary, four residual tumors, and four lymph node or distant metastases) was investigated by conventional histology and scanning DNA Cytophotometry. Of the 60 TC (96%), 58 exhibited euploid DNA histograms compared with only 20 (50%) of the 40 AC. The morphologic findings were related to the patients' survival (median observation period, 9 years). Statistical analyses disclosed the histologic type of disease (TC versus AC) and the DNA content of tumors (euploid versus aneuploid) to affect prognosis significantly (p < 0.001). Deaths resulting from tumor were exclusively observed among patients with atypical (eight of 40) or DNA aneuploid carcinoids (eight of 22). Six patients were alive with persistent tumor manifestations 3 to 20 years after initial diagnosis, four with DNA diploid primary carcinoids. The presence of lymph node metastases alone was not associated with poor prognosis as long as the primary tumor or the related metastases showed a diploid DNA content. DNA Cytophotometry thus might be regarded as an adjunctive prognostic criterion in individual carcinoid cases.
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DNA Cytophotometry and prognosis in ovarian tumors of borderline malignancy. A clinicomorphologic study of 80 cases
Cancer, 1992Co-Authors: Barbara-christina Padberg, Hartmut Arps, Sören Schröder, Ursula Franke, Carsten Thiedemann, Wolfgang Rehpenning, Hans-egon Stegner, Helmut Lietz, Manfred DietelAbstract:Surgical specimens of 80 ovarian tumors of borderline malignancy (OTBM) were investigated by scanning DNA Cytophotometry. Diploid or euploid DNA histograms were found for 21 tumors, whereas 59 OTBM showed noneuploid or aneuploid DNA patterns. All patients were followed-up after surgery for at least 3 years (mean observation period, 6.7 years). Follow-up showed 11 cases of recurrent disease and 6 deaths. DNA findings and several other morphologic and clinical details (including patient age, histologic type and stage of disease, and extent of therapy) were correlated to the postoperative course. Statistical analyses disclosed that, of these parameters, only DNA content significantly affected prognosis. Recurrences and deaths resulting from tumor exclusively were observed among patients with noneuploid or aneuploid OTBM, whereas none of the diploid or euploid tumors recurred (P less than 0.05). DNA Cytophotometry thus might be regarded as an effective complementary means to assess the prognosis of individual OTBM cases.
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DNA Cytophotometry in adrenocortical tumours: A clinicomorphological study of 66 cases
Virchows Archiv A, 1991Co-Authors: Barbara-christina Padberg, Ines Lauritzen, Eike Achilles, Konstanze Holl, Max Bressel, Günter Klöppel, Henning Dralle, Sören SchröderAbstract:Surgical specimens of 66 adrenocortical tumours were investigated by conventional microscopy and DNA Cytophotometry. Histologically, 50 neoplasms were classified as adenomas and 16 as carcinomas. In only 8 of the latter cases were distant metastases and/or a lethal outcome recorded. On single cell scanning Cytophotometry either non-euploid or aneuploid DNA histograms were identified in 24 of 50 adenomas (48%) and in 14 of 16 carcinomas (88%). The two carcinomas exhibiting euploid DNA distributions fell into the group of 7 malignancies which are recurrence-free so far. From these findings it is concluded that DNA measurements have no diagnostic and only limited prognostic value in neoplasms of the adrenal cortex.
Maria G. Giacobini Robecchi - One of the best experts on this subject based on the ideXlab platform.
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DNA content revealed by Cytophotometry in neurons: variability related to neuroplasticity.
Advances in experimental medicine and biology, 1991Co-Authors: Stefano Geuna, A. Poncino, Maria G. Giacobini RobecchiAbstract:Cytophotometry after Feulgen staining provided a new tool for the determination of nuclear DNA content. Controversial data on the DNA content of neurons in different species of animals, as well as in different regions of the central and peripheral nervous system, have been reported.
Stefano Geuna - One of the best experts on this subject based on the ideXlab platform.
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DNA content revealed by Cytophotometry in neurons: variability related to neuroplasticity.
Advances in experimental medicine and biology, 1991Co-Authors: Stefano Geuna, A. Poncino, Maria G. Giacobini RobecchiAbstract:Cytophotometry after Feulgen staining provided a new tool for the determination of nuclear DNA content. Controversial data on the DNA content of neurons in different species of animals, as well as in different regions of the central and peripheral nervous system, have been reported.
D Gennai - One of the best experts on this subject based on the ideXlab platform.
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Nuclear DNA variability within Pisum sativum (Leguminosae): nucleotypic effects on plant growth
Heredity, 1993Co-Authors: A Cavallini, L Natali, G Cionini, D GennaiAbstract:Nuclear DNA content variability was established among seven cultivars of experimental lines of pea ( Pisum sativum L.) by means of Feulgen Cytophotometry. In order to study the phenotypic effects of such DNA content variation, different parameters were tested. A positive correlation with root and stem growth was determined during the first developmental stages after germination. In particular, differential response in plant growth seemed to be correlated with differences in enlargement of cells preexisting in the embryo, while no difference was observed in mitotic activity. The results are discussed in relation to the effects of nucleotype on cell and plant growth.
