The Experts below are selected from a list of 213 Experts worldwide ranked by ideXlab platform
Haiyan Xu - One of the best experts on this subject based on the ideXlab platform.
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Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-Cytotoxic Concentrations
Particle and Fibre Toxicology, 2019Co-Authors: Tao Wen, Lifan Du, Doudou Yan, Aiyun Yang, Ning Gu, Jie Meng, Jian Liu, Bo Chen, Haiyan XuAbstract:BackgroundIron oxide nanoparticles (IONPs) have been extensively studied in different biomedical fields. Recently, the non-Cytotoxic Concentration of IONPs induced cell-specific response raised concern of their safety. Endothelial cell exposure was unavoidable in their applications, while whether IONPs affect the phenotype of vascular endothelial cells is largely unknown. In this work, the effect of IONPs on endothelial-to-mesenchymal transition (EndMT) was investigated in vitro and in vivo.ResultsThe incubation with γ-Fe_2O_3 nanoparticles modified with polyglucose sorbitol carboxymethyether (PSC-Fe_2O_3) at non-Cytotoxic Concentration induced morphological changes of human umbilical vein endothelial cells (HUVECs) from cobblestone-like to spindle mesenchymal-like morphology, while PSC-Fe_2O_3 mostly stay in the culture medium and intercellular space. At the same time, the endothelial marker CD31 and VE-cadherin was decreased along with the inhibitory of angiogenesis properties of HUVEC. Meanwhile, the mesenchymal marker α-smooth muscle actin (α-SMA) and fibroblast specific protein (FSP) was up regulated significantly, and the migration ability of the cells was enhanced. When ROS scavenger mannitol or AA was supplemented, the EndMT was rescued. Results from the in vivo study showed that, expression of CD31 was decreased and α-SMA increased in the liver, spleen and kidney of mice given PSC-Fe_2O_3, and the density of collagen fibers in the liver sinusoid of mice was increased. The supplementary mannitol or AA could reverse the degree of EndMT in the tissues. Mechanistic study in vitro indicated that the level of extracellular hydroxyl radicals (·OH) was up regulated significantly by PSC-Fe_2O_3, which induced the response of intracellular ROS and resulted in the EndMT effect on HUVECs.ConclusionThe PSC-Fe_2O_3 was capable of inducing EndMT in the endothelial cells at acutely non-Cytotoxic dose due to its intrinsic peroxidase-like activity, though they were few taken up by endothelial cell. The EndMT effect on HUVEC can be rescued by ROS scavenger in vitro and in vivo.
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Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-Cytotoxic Concentrations
Particle and Fibre Toxicology, 2019Co-Authors: Lifan Du, Aiyun Yang, Ning Gu, Jie Meng, Bo Chen, Haiyan XuAbstract:Iron oxide nanoparticles (IONPs) have been extensively studied in different biomedical fields. Recently, the non-Cytotoxic Concentration of IONPs induced cell-specific response raised concern of their safety. Endothelial cell exposure was unavoidable in their applications, while whether IONPs affect the phenotype of vascular endothelial cells is largely unknown. In this work, the effect of IONPs on endothelial-to-mesenchymal transition (EndMT) was investigated in vitro and in vivo. The incubation with γ-Fe2O3 nanoparticles modified with polyglucose sorbitol carboxymethyether (PSC-Fe2O3) at non-Cytotoxic Concentration induced morphological changes of human umbilical vein endothelial cells (HUVECs) from cobblestone-like to spindle mesenchymal-like morphology, while PSC-Fe2O3 mostly stay in the culture medium and intercellular space. At the same time, the endothelial marker CD31 and VE-cadherin was decreased along with the inhibitory of angiogenesis properties of HUVEC. Meanwhile, the mesenchymal marker α-smooth muscle actin (α-SMA) and fibroblast specific protein (FSP) was up regulated significantly, and the migration ability of the cells was enhanced. When ROS scavenger mannitol or AA was supplemented, the EndMT was rescued. Results from the in vivo study showed that, expression of CD31 was decreased and α-SMA increased in the liver, spleen and kidney of mice given PSC-Fe2O3, and the density of collagen fibers in the liver sinusoid of mice was increased. The supplementary mannitol or AA could reverse the degree of EndMT in the tissues. Mechanistic study in vitro indicated that the level of extracellular hydroxyl radicals (·OH) was up regulated significantly by PSC-Fe2O3, which induced the response of intracellular ROS and resulted in the EndMT effect on HUVECs. The PSC-Fe2O3 was capable of inducing EndMT in the endothelial cells at acutely non-Cytotoxic dose due to its intrinsic peroxidase-like activity, though they were few taken up by endothelial cell. The EndMT effect on HUVEC can be rescued by ROS scavenger in vitro and in vivo.
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Comparative study of in vitro effects of different nanoparticles at non-Cytotoxic Concentration on the adherens junction of human vascular endothelial cells
International Journal of Nanomedicine, 2019Co-Authors: Aiyun Yang, Lifan Du, Jie Meng, Lingyu Piao, Haiyan XuAbstract:Effects of different nanoparticles (NPs) exposure at acutely non-Cytotoxic Concentrations are particularly worthy to figure out, compare, and elucidate. To investigate and compare the effect of a small library of NPs at non-Cytotoxic Concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium dioxide (TiO2), ferric oxide (Fe2O3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the Cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. The acutely non-Cytotoxic Concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-Cytotoxic Concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.
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comparative study of in vitro effects of different nanoparticles at non Cytotoxic Concentration on the adherens junction of human vascular endothelial cells
International Journal of Nanomedicine, 2019Co-Authors: Aiyun Yang, Lifan Du, Jie Meng, Lingyu Piao, Haiyan XuAbstract:Background: Effects of different nanoparticles (NPs) exposure at acutely non-Cytotoxic Concentrations are particularly worthy to figure out, compare, and elucidate. Objective: To investigate and compare the effect of a small library of NPs at non-Cytotoxic Concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. Materials and methods: The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium dioxide (TiO2), ferric oxide (Fe2O3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the Cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. Results: The acutely non-Cytotoxic Concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. Conclusion: The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-Cytotoxic Concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.
Aiyun Yang - One of the best experts on this subject based on the ideXlab platform.
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Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-Cytotoxic Concentrations
Particle and Fibre Toxicology, 2019Co-Authors: Tao Wen, Lifan Du, Doudou Yan, Aiyun Yang, Ning Gu, Jie Meng, Jian Liu, Bo Chen, Haiyan XuAbstract:BackgroundIron oxide nanoparticles (IONPs) have been extensively studied in different biomedical fields. Recently, the non-Cytotoxic Concentration of IONPs induced cell-specific response raised concern of their safety. Endothelial cell exposure was unavoidable in their applications, while whether IONPs affect the phenotype of vascular endothelial cells is largely unknown. In this work, the effect of IONPs on endothelial-to-mesenchymal transition (EndMT) was investigated in vitro and in vivo.ResultsThe incubation with γ-Fe_2O_3 nanoparticles modified with polyglucose sorbitol carboxymethyether (PSC-Fe_2O_3) at non-Cytotoxic Concentration induced morphological changes of human umbilical vein endothelial cells (HUVECs) from cobblestone-like to spindle mesenchymal-like morphology, while PSC-Fe_2O_3 mostly stay in the culture medium and intercellular space. At the same time, the endothelial marker CD31 and VE-cadherin was decreased along with the inhibitory of angiogenesis properties of HUVEC. Meanwhile, the mesenchymal marker α-smooth muscle actin (α-SMA) and fibroblast specific protein (FSP) was up regulated significantly, and the migration ability of the cells was enhanced. When ROS scavenger mannitol or AA was supplemented, the EndMT was rescued. Results from the in vivo study showed that, expression of CD31 was decreased and α-SMA increased in the liver, spleen and kidney of mice given PSC-Fe_2O_3, and the density of collagen fibers in the liver sinusoid of mice was increased. The supplementary mannitol or AA could reverse the degree of EndMT in the tissues. Mechanistic study in vitro indicated that the level of extracellular hydroxyl radicals (·OH) was up regulated significantly by PSC-Fe_2O_3, which induced the response of intracellular ROS and resulted in the EndMT effect on HUVECs.ConclusionThe PSC-Fe_2O_3 was capable of inducing EndMT in the endothelial cells at acutely non-Cytotoxic dose due to its intrinsic peroxidase-like activity, though they were few taken up by endothelial cell. The EndMT effect on HUVEC can be rescued by ROS scavenger in vitro and in vivo.
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Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-Cytotoxic Concentrations
Particle and Fibre Toxicology, 2019Co-Authors: Lifan Du, Aiyun Yang, Ning Gu, Jie Meng, Bo Chen, Haiyan XuAbstract:Iron oxide nanoparticles (IONPs) have been extensively studied in different biomedical fields. Recently, the non-Cytotoxic Concentration of IONPs induced cell-specific response raised concern of their safety. Endothelial cell exposure was unavoidable in their applications, while whether IONPs affect the phenotype of vascular endothelial cells is largely unknown. In this work, the effect of IONPs on endothelial-to-mesenchymal transition (EndMT) was investigated in vitro and in vivo. The incubation with γ-Fe2O3 nanoparticles modified with polyglucose sorbitol carboxymethyether (PSC-Fe2O3) at non-Cytotoxic Concentration induced morphological changes of human umbilical vein endothelial cells (HUVECs) from cobblestone-like to spindle mesenchymal-like morphology, while PSC-Fe2O3 mostly stay in the culture medium and intercellular space. At the same time, the endothelial marker CD31 and VE-cadherin was decreased along with the inhibitory of angiogenesis properties of HUVEC. Meanwhile, the mesenchymal marker α-smooth muscle actin (α-SMA) and fibroblast specific protein (FSP) was up regulated significantly, and the migration ability of the cells was enhanced. When ROS scavenger mannitol or AA was supplemented, the EndMT was rescued. Results from the in vivo study showed that, expression of CD31 was decreased and α-SMA increased in the liver, spleen and kidney of mice given PSC-Fe2O3, and the density of collagen fibers in the liver sinusoid of mice was increased. The supplementary mannitol or AA could reverse the degree of EndMT in the tissues. Mechanistic study in vitro indicated that the level of extracellular hydroxyl radicals (·OH) was up regulated significantly by PSC-Fe2O3, which induced the response of intracellular ROS and resulted in the EndMT effect on HUVECs. The PSC-Fe2O3 was capable of inducing EndMT in the endothelial cells at acutely non-Cytotoxic dose due to its intrinsic peroxidase-like activity, though they were few taken up by endothelial cell. The EndMT effect on HUVEC can be rescued by ROS scavenger in vitro and in vivo.
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Comparative study of in vitro effects of different nanoparticles at non-Cytotoxic Concentration on the adherens junction of human vascular endothelial cells
International Journal of Nanomedicine, 2019Co-Authors: Aiyun Yang, Lifan Du, Jie Meng, Lingyu Piao, Haiyan XuAbstract:Effects of different nanoparticles (NPs) exposure at acutely non-Cytotoxic Concentrations are particularly worthy to figure out, compare, and elucidate. To investigate and compare the effect of a small library of NPs at non-Cytotoxic Concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium dioxide (TiO2), ferric oxide (Fe2O3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the Cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. The acutely non-Cytotoxic Concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-Cytotoxic Concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.
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comparative study of in vitro effects of different nanoparticles at non Cytotoxic Concentration on the adherens junction of human vascular endothelial cells
International Journal of Nanomedicine, 2019Co-Authors: Aiyun Yang, Lifan Du, Jie Meng, Lingyu Piao, Haiyan XuAbstract:Background: Effects of different nanoparticles (NPs) exposure at acutely non-Cytotoxic Concentrations are particularly worthy to figure out, compare, and elucidate. Objective: To investigate and compare the effect of a small library of NPs at non-Cytotoxic Concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. Materials and methods: The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium dioxide (TiO2), ferric oxide (Fe2O3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the Cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. Results: The acutely non-Cytotoxic Concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. Conclusion: The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-Cytotoxic Concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.
Lifan Du - One of the best experts on this subject based on the ideXlab platform.
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Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-Cytotoxic Concentrations
Particle and Fibre Toxicology, 2019Co-Authors: Tao Wen, Lifan Du, Doudou Yan, Aiyun Yang, Ning Gu, Jie Meng, Jian Liu, Bo Chen, Haiyan XuAbstract:BackgroundIron oxide nanoparticles (IONPs) have been extensively studied in different biomedical fields. Recently, the non-Cytotoxic Concentration of IONPs induced cell-specific response raised concern of their safety. Endothelial cell exposure was unavoidable in their applications, while whether IONPs affect the phenotype of vascular endothelial cells is largely unknown. In this work, the effect of IONPs on endothelial-to-mesenchymal transition (EndMT) was investigated in vitro and in vivo.ResultsThe incubation with γ-Fe_2O_3 nanoparticles modified with polyglucose sorbitol carboxymethyether (PSC-Fe_2O_3) at non-Cytotoxic Concentration induced morphological changes of human umbilical vein endothelial cells (HUVECs) from cobblestone-like to spindle mesenchymal-like morphology, while PSC-Fe_2O_3 mostly stay in the culture medium and intercellular space. At the same time, the endothelial marker CD31 and VE-cadherin was decreased along with the inhibitory of angiogenesis properties of HUVEC. Meanwhile, the mesenchymal marker α-smooth muscle actin (α-SMA) and fibroblast specific protein (FSP) was up regulated significantly, and the migration ability of the cells was enhanced. When ROS scavenger mannitol or AA was supplemented, the EndMT was rescued. Results from the in vivo study showed that, expression of CD31 was decreased and α-SMA increased in the liver, spleen and kidney of mice given PSC-Fe_2O_3, and the density of collagen fibers in the liver sinusoid of mice was increased. The supplementary mannitol or AA could reverse the degree of EndMT in the tissues. Mechanistic study in vitro indicated that the level of extracellular hydroxyl radicals (·OH) was up regulated significantly by PSC-Fe_2O_3, which induced the response of intracellular ROS and resulted in the EndMT effect on HUVECs.ConclusionThe PSC-Fe_2O_3 was capable of inducing EndMT in the endothelial cells at acutely non-Cytotoxic dose due to its intrinsic peroxidase-like activity, though they were few taken up by endothelial cell. The EndMT effect on HUVEC can be rescued by ROS scavenger in vitro and in vivo.
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Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-Cytotoxic Concentrations
Particle and Fibre Toxicology, 2019Co-Authors: Lifan Du, Aiyun Yang, Ning Gu, Jie Meng, Bo Chen, Haiyan XuAbstract:Iron oxide nanoparticles (IONPs) have been extensively studied in different biomedical fields. Recently, the non-Cytotoxic Concentration of IONPs induced cell-specific response raised concern of their safety. Endothelial cell exposure was unavoidable in their applications, while whether IONPs affect the phenotype of vascular endothelial cells is largely unknown. In this work, the effect of IONPs on endothelial-to-mesenchymal transition (EndMT) was investigated in vitro and in vivo. The incubation with γ-Fe2O3 nanoparticles modified with polyglucose sorbitol carboxymethyether (PSC-Fe2O3) at non-Cytotoxic Concentration induced morphological changes of human umbilical vein endothelial cells (HUVECs) from cobblestone-like to spindle mesenchymal-like morphology, while PSC-Fe2O3 mostly stay in the culture medium and intercellular space. At the same time, the endothelial marker CD31 and VE-cadherin was decreased along with the inhibitory of angiogenesis properties of HUVEC. Meanwhile, the mesenchymal marker α-smooth muscle actin (α-SMA) and fibroblast specific protein (FSP) was up regulated significantly, and the migration ability of the cells was enhanced. When ROS scavenger mannitol or AA was supplemented, the EndMT was rescued. Results from the in vivo study showed that, expression of CD31 was decreased and α-SMA increased in the liver, spleen and kidney of mice given PSC-Fe2O3, and the density of collagen fibers in the liver sinusoid of mice was increased. The supplementary mannitol or AA could reverse the degree of EndMT in the tissues. Mechanistic study in vitro indicated that the level of extracellular hydroxyl radicals (·OH) was up regulated significantly by PSC-Fe2O3, which induced the response of intracellular ROS and resulted in the EndMT effect on HUVECs. The PSC-Fe2O3 was capable of inducing EndMT in the endothelial cells at acutely non-Cytotoxic dose due to its intrinsic peroxidase-like activity, though they were few taken up by endothelial cell. The EndMT effect on HUVEC can be rescued by ROS scavenger in vitro and in vivo.
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Comparative study of in vitro effects of different nanoparticles at non-Cytotoxic Concentration on the adherens junction of human vascular endothelial cells
International Journal of Nanomedicine, 2019Co-Authors: Aiyun Yang, Lifan Du, Jie Meng, Lingyu Piao, Haiyan XuAbstract:Effects of different nanoparticles (NPs) exposure at acutely non-Cytotoxic Concentrations are particularly worthy to figure out, compare, and elucidate. To investigate and compare the effect of a small library of NPs at non-Cytotoxic Concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium dioxide (TiO2), ferric oxide (Fe2O3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the Cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. The acutely non-Cytotoxic Concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-Cytotoxic Concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.
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comparative study of in vitro effects of different nanoparticles at non Cytotoxic Concentration on the adherens junction of human vascular endothelial cells
International Journal of Nanomedicine, 2019Co-Authors: Aiyun Yang, Lifan Du, Jie Meng, Lingyu Piao, Haiyan XuAbstract:Background: Effects of different nanoparticles (NPs) exposure at acutely non-Cytotoxic Concentrations are particularly worthy to figure out, compare, and elucidate. Objective: To investigate and compare the effect of a small library of NPs at non-Cytotoxic Concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. Materials and methods: The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium dioxide (TiO2), ferric oxide (Fe2O3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the Cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. Results: The acutely non-Cytotoxic Concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. Conclusion: The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-Cytotoxic Concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.
Jie Meng - One of the best experts on this subject based on the ideXlab platform.
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Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-Cytotoxic Concentrations
Particle and Fibre Toxicology, 2019Co-Authors: Tao Wen, Lifan Du, Doudou Yan, Aiyun Yang, Ning Gu, Jie Meng, Jian Liu, Bo Chen, Haiyan XuAbstract:BackgroundIron oxide nanoparticles (IONPs) have been extensively studied in different biomedical fields. Recently, the non-Cytotoxic Concentration of IONPs induced cell-specific response raised concern of their safety. Endothelial cell exposure was unavoidable in their applications, while whether IONPs affect the phenotype of vascular endothelial cells is largely unknown. In this work, the effect of IONPs on endothelial-to-mesenchymal transition (EndMT) was investigated in vitro and in vivo.ResultsThe incubation with γ-Fe_2O_3 nanoparticles modified with polyglucose sorbitol carboxymethyether (PSC-Fe_2O_3) at non-Cytotoxic Concentration induced morphological changes of human umbilical vein endothelial cells (HUVECs) from cobblestone-like to spindle mesenchymal-like morphology, while PSC-Fe_2O_3 mostly stay in the culture medium and intercellular space. At the same time, the endothelial marker CD31 and VE-cadherin was decreased along with the inhibitory of angiogenesis properties of HUVEC. Meanwhile, the mesenchymal marker α-smooth muscle actin (α-SMA) and fibroblast specific protein (FSP) was up regulated significantly, and the migration ability of the cells was enhanced. When ROS scavenger mannitol or AA was supplemented, the EndMT was rescued. Results from the in vivo study showed that, expression of CD31 was decreased and α-SMA increased in the liver, spleen and kidney of mice given PSC-Fe_2O_3, and the density of collagen fibers in the liver sinusoid of mice was increased. The supplementary mannitol or AA could reverse the degree of EndMT in the tissues. Mechanistic study in vitro indicated that the level of extracellular hydroxyl radicals (·OH) was up regulated significantly by PSC-Fe_2O_3, which induced the response of intracellular ROS and resulted in the EndMT effect on HUVECs.ConclusionThe PSC-Fe_2O_3 was capable of inducing EndMT in the endothelial cells at acutely non-Cytotoxic dose due to its intrinsic peroxidase-like activity, though they were few taken up by endothelial cell. The EndMT effect on HUVEC can be rescued by ROS scavenger in vitro and in vivo.
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Iron oxide nanoparticles induce reversible endothelial-to-mesenchymal transition in vascular endothelial cells at acutely non-Cytotoxic Concentrations
Particle and Fibre Toxicology, 2019Co-Authors: Lifan Du, Aiyun Yang, Ning Gu, Jie Meng, Bo Chen, Haiyan XuAbstract:Iron oxide nanoparticles (IONPs) have been extensively studied in different biomedical fields. Recently, the non-Cytotoxic Concentration of IONPs induced cell-specific response raised concern of their safety. Endothelial cell exposure was unavoidable in their applications, while whether IONPs affect the phenotype of vascular endothelial cells is largely unknown. In this work, the effect of IONPs on endothelial-to-mesenchymal transition (EndMT) was investigated in vitro and in vivo. The incubation with γ-Fe2O3 nanoparticles modified with polyglucose sorbitol carboxymethyether (PSC-Fe2O3) at non-Cytotoxic Concentration induced morphological changes of human umbilical vein endothelial cells (HUVECs) from cobblestone-like to spindle mesenchymal-like morphology, while PSC-Fe2O3 mostly stay in the culture medium and intercellular space. At the same time, the endothelial marker CD31 and VE-cadherin was decreased along with the inhibitory of angiogenesis properties of HUVEC. Meanwhile, the mesenchymal marker α-smooth muscle actin (α-SMA) and fibroblast specific protein (FSP) was up regulated significantly, and the migration ability of the cells was enhanced. When ROS scavenger mannitol or AA was supplemented, the EndMT was rescued. Results from the in vivo study showed that, expression of CD31 was decreased and α-SMA increased in the liver, spleen and kidney of mice given PSC-Fe2O3, and the density of collagen fibers in the liver sinusoid of mice was increased. The supplementary mannitol or AA could reverse the degree of EndMT in the tissues. Mechanistic study in vitro indicated that the level of extracellular hydroxyl radicals (·OH) was up regulated significantly by PSC-Fe2O3, which induced the response of intracellular ROS and resulted in the EndMT effect on HUVECs. The PSC-Fe2O3 was capable of inducing EndMT in the endothelial cells at acutely non-Cytotoxic dose due to its intrinsic peroxidase-like activity, though they were few taken up by endothelial cell. The EndMT effect on HUVEC can be rescued by ROS scavenger in vitro and in vivo.
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Comparative study of in vitro effects of different nanoparticles at non-Cytotoxic Concentration on the adherens junction of human vascular endothelial cells
International Journal of Nanomedicine, 2019Co-Authors: Aiyun Yang, Lifan Du, Jie Meng, Lingyu Piao, Haiyan XuAbstract:Effects of different nanoparticles (NPs) exposure at acutely non-Cytotoxic Concentrations are particularly worthy to figure out, compare, and elucidate. To investigate and compare the effect of a small library of NPs at non-Cytotoxic Concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium dioxide (TiO2), ferric oxide (Fe2O3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the Cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. The acutely non-Cytotoxic Concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-Cytotoxic Concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.
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comparative study of in vitro effects of different nanoparticles at non Cytotoxic Concentration on the adherens junction of human vascular endothelial cells
International Journal of Nanomedicine, 2019Co-Authors: Aiyun Yang, Lifan Du, Jie Meng, Lingyu Piao, Haiyan XuAbstract:Background: Effects of different nanoparticles (NPs) exposure at acutely non-Cytotoxic Concentrations are particularly worthy to figure out, compare, and elucidate. Objective: To investigate and compare the effect of a small library of NPs at non-Cytotoxic Concentration on the adherens junction of human umbilical vein endothelial cells (HUVECs), obtaining new insights of NPs safety evaluation. Materials and methods: The HUVECs layer was exposed to NPs including gold (Au), platinum (Pt), silica (SiO2), titanium dioxide (TiO2), ferric oxide (Fe2O3), oxidized multi-walled carbon nanotubes, with different surface chemistry and size distribution. Cellular uptake of NPs was observed by transmission electron microscopy. and the Cytotoxicity was determined by Cell Counting Kit-8 assay. The NP-induced variation of intracellular reactive oxygen species (ROS) and catalase (CAT) activity was measured using the probe of 2'7'-dichlorodihydr fluorescein diacetate and a CAT analysis kit, respectively. The level of VE-cadherin of HUVECs was analyzed by Western blot, and the loss of adherens junction was observed with laser confocal microscopy. Results: The acutely non-Cytotoxic Concentrations of different NPs were determined and applied to HUVECs. The NPs increased the level of intracellular ROS and the activity of CAT to different degrees, depending on the characteristics. At the same time, the HUVECs lost their adherens junction protein VE-cadherin and gaps were formed between the cells. The NP-induced oxidative stress and gap formation could be rescued by the supplementary N-acetylcysteine in the incubation. Conclusion: The increase of intracellular ROS and CAT activity was one common effect of NPs, even at the non-Cytotoxic Concentration, and the degree was dependent on the composition, surface chemistry, and size distribution of the NP. The effect led to the gap formation between the cells, while could be rescued by the antioxidant. Therefore, the variation of adherens junction between endothelial cells was suggested to evaluate for NPs when used as therapeutics and diagnostics.
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evaluation of Cytotoxic Concentration time response in a549 cells exposed to respirable α quartz
Journal of Applied Toxicology, 2009Co-Authors: Carla Fanizza, Anna Maria Fresegna, Raffaele Maiello, Emilia Paba, Delia CavalloAbstract:A causal pathway between quartz, silicosis and lung cancer has been postulated. The aim of our study was to assess Cytotoxic effects induced in a human lung epithelial cell line (A549) by exposure to α-quartz. Cells were exposed to respirable α-quartz (SRM1878a, NIST) at 25, 50 or 100 µg ml−1 for 24 h and at 50 or 100 µg ml−1 for 48 h. Cytotoxic effects were analyzed by scanning electron microscopy (SEM), apoptotic morphology analysis with Hoechst staining and lactate dehydrogenase (LDH) release assay. In cells exposed to α-quartz for 24 h, a Concentration-dependent bleb development and in particular the localization of blebs at the cell edge at higher Concentrations were observed. The blebbing phenomenon was more evident after 48 h of exposure to 50 or to 100 µg ml−1 of α-quartz and large blebs were localized at the cell edge. At the same Concentrations surface smoothing was also observed. Moreover the presence of holes and tears was detected at the highest Concentration both at 24 and 48 h. Results of morphological analysis with Hoechst stain evidenced an increase Concentration–time dependent of apoptotic cell percentage that was more marked after 48 h exposure to 100 µg ml−1 and a prevalence of late apoptosis stage with the increase of exposure time and Concentration. Cells exposed to 50 or 100 µg ml−1 of α-quartz for 24 and 48 h produced a significant increase in LDH release. The Concentration–time-dependent bleb induction evidenced by SEM correlates with the increase of apoptotic cells and LDH activity release, demonstrating the onset of Cytotoxic effects in human lung cells exposed to α-quartz. Copyright © 2009 John Wiley & Sons, Ltd.
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Evaluation of Cytotoxic Concentration–time response in A549 cells exposed to respirable α‐quartz
Journal of Applied Toxicology, 2009Co-Authors: Carla Fanizza, Anna Maria Fresegna, Raffaele Maiello, Emilia Paba, Delia CavalloAbstract:A causal pathway between quartz, silicosis and lung cancer has been postulated. The aim of our study was to assess Cytotoxic effects induced in a human lung epithelial cell line (A549) by exposure to α-quartz. Cells were exposed to respirable α-quartz (SRM1878a, NIST) at 25, 50 or 100 µg ml−1 for 24 h and at 50 or 100 µg ml−1 for 48 h. Cytotoxic effects were analyzed by scanning electron microscopy (SEM), apoptotic morphology analysis with Hoechst staining and lactate dehydrogenase (LDH) release assay. In cells exposed to α-quartz for 24 h, a Concentration-dependent bleb development and in particular the localization of blebs at the cell edge at higher Concentrations were observed. The blebbing phenomenon was more evident after 48 h of exposure to 50 or to 100 µg ml−1 of α-quartz and large blebs were localized at the cell edge. At the same Concentrations surface smoothing was also observed. Moreover the presence of holes and tears was detected at the highest Concentration both at 24 and 48 h. Results of morphological analysis with Hoechst stain evidenced an increase Concentration–time dependent of apoptotic cell percentage that was more marked after 48 h exposure to 100 µg ml−1 and a prevalence of late apoptosis stage with the increase of exposure time and Concentration. Cells exposed to 50 or 100 µg ml−1 of α-quartz for 24 and 48 h produced a significant increase in LDH release. The Concentration–time-dependent bleb induction evidenced by SEM correlates with the increase of apoptotic cells and LDH activity release, demonstrating the onset of Cytotoxic effects in human lung cells exposed to α-quartz. Copyright © 2009 John Wiley & Sons, Ltd.
