The Experts below are selected from a list of 170496 Experts worldwide ranked by ideXlab platform
Ryu Miura - One of the best experts on this subject based on the ideXlab platform.
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an optimal Data Collection Technique for improved utility in uas aided networks
International Conference on Computer Communications, 2014Co-Authors: Ahmed E A A Abdulla, Zubair Md Fadlullah, Hiroki Nishiyama, Nei Kato, Fumie Ono, Ryu MiuraAbstract:Recent technological advances in electronics, sen- sors, and communications devices have facilitated the prolifer- ation of Unmanned Aircraft System (UAS)-aided applications. However, the UAS-aided communications networks are yet to receive sufficient research endeavor. In this paper, we address one of the most important research challenges pertaining to UAS-aided networks comprising adaptive modulation-capable nodes, namely how to fairly maximize the energy efficiency (throughput per energy). For the mobility pattern innate to the UAS, we demonstrate how the adaptive modulation behaves. Furthermore, we formulate the problem as a potential game that is played between the UAS and the network-nodes, and prove its stability, optimality, and convergence. Based upon the potential game, a Data Collection method is envisioned to maximize the energy efficiency with the fairness constraint. Additionally, we analyze the Price of Anarchy (PoA) of our proposed game. Extensive simulations exhibit the effectiveness of our proposal under varying environments. sensor nodes require only capabilities to communicate with the CHs. The mobility pattern of the UAS causes the distance between a CH and the UAS to vary. The distance between the CH and the UAS affects the Signal-to-Noise Ratio (SNR), which in turn affects the Bit Error Rate (BER) of the CH transmissions. Both SNR and BER affect the modulation scheme. This is because modulation schemes that transmit more bits per symbol require higher values of SNR for a given BER requirement (9). Moreover, if high levels of BER are acceptable, the achievable number of bits per symbol that a modulation scheme transmits can be increased.
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INFOCOM - An Optimal Data Collection Technique for Improved Utility in UAS-aided Networks
IEEE INFOCOM 2014 - IEEE Conference on Computer Communications, 2014Co-Authors: Ahmed E A A Abdulla, Zubair Md Fadlullah, Hiroki Nishiyama, Nei Kato, Fumie Ono, Ryu MiuraAbstract:Recent technological advances in electronics, sen- sors, and communications devices have facilitated the prolifer- ation of Unmanned Aircraft System (UAS)-aided applications. However, the UAS-aided communications networks are yet to receive sufficient research endeavor. In this paper, we address one of the most important research challenges pertaining to UAS-aided networks comprising adaptive modulation-capable nodes, namely how to fairly maximize the energy efficiency (throughput per energy). For the mobility pattern innate to the UAS, we demonstrate how the adaptive modulation behaves. Furthermore, we formulate the problem as a potential game that is played between the UAS and the network-nodes, and prove its stability, optimality, and convergence. Based upon the potential game, a Data Collection method is envisioned to maximize the energy efficiency with the fairness constraint. Additionally, we analyze the Price of Anarchy (PoA) of our proposed game. Extensive simulations exhibit the effectiveness of our proposal under varying environments. sensor nodes require only capabilities to communicate with the CHs. The mobility pattern of the UAS causes the distance between a CH and the UAS to vary. The distance between the CH and the UAS affects the Signal-to-Noise Ratio (SNR), which in turn affects the Bit Error Rate (BER) of the CH transmissions. Both SNR and BER affect the modulation scheme. This is because modulation schemes that transmit more bits per symbol require higher values of SNR for a given BER requirement (9). Moreover, if high levels of BER are acceptable, the achievable number of bits per symbol that a modulation scheme transmits can be increased.
Ying-yong Zhao - One of the best experts on this subject based on the ideXlab platform.
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renal metabolic profiling of early renal injury and renoprotective effects of poria cocos epidermis using uplc q tof hsms mse
Journal of Pharmaceutical and Biomedical Analysis, 2013Co-Authors: Ying-yong Zhao, Yalong Feng, Ping Lei, Danqian Chen, Xu BaiAbstract:Poria cocos epidermis is one of ancient traditional Chinese medicines (TCMs), which is usually used for the treatment of chronic kidney disease (CKD) for thousands of years in China. A metabonomic approach based on ultra performance liquid chromatography coupled with quadrupole time-of-flight high-sensitivity mass spectrometry (UPLC Q-TOF/HSMS) and a mass spectrometry(Elevated Energy) (MS(E)) Data Collection Technique was developed to obtained a systematic view of the development and progression of CKD and biochemistry mechanism of therapeutic effects of P. cocos epidermis (Fu-Ling-Pi, FLP). By partial least squares-discriminate analysis, 19 metabolites were identified as potential biomarkers of CKD. Among the 19 biomarkers, 10 biomarkers including eicosapentaenoic acid, docosahexaenoic acid, lysoPC(20:4), lysoPC(18:2), lysoPC(15:0), lysoPE(20:0/0:0), indoxyl sulfate, hippuric acid, p-cresol sulfate and allantoin were reversed to the control level in FLP-treated groups. The study indicates that FLP treatment can ameliorate CKD by intervening in some dominating metabolic pathways, such as fatty acid metabolism, phospholipid metabolism, purine metabolism and tryptophan metabolism. This work was for the first time to investigate the FLP therapeutic effect based on metabonomics technology, which is a potentially powerful tool to study the TCMs.
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ultra performance liquid chromatography based metabonomic study of therapeutic effect of the surface layer of poria cocos on adenine induced chronic kidney disease provides new insight into anti fibrosis mechanism
PLOS ONE, 2013Co-Authors: Ying-yong Zhao, Xu Bai, Rui-chao Lin, Yalong Feng, Xiaojie Tan, Qibing MeiAbstract:The surface layer of Poria cocos (Fu-Ling-Pi, FLP) is commonly used in traditional Chinese medicine and its diuretic effect was confirmed in rat. Ultra performance liquid chromatography/quadrupole time-of-flight high-sensitivity mass spectrometry and a novel mass spectrometryElevated Energy Data Collection Technique was employed to investigate metabonomic characteristics of chronic kidney disease (CKD) induced from adenine excess and the protective effects of FLP. Multiple metabolites are detected in the CKD and are correlated with progressive renal injury. Among these biomarkers, lysoPC(18∶0), tetracosahexaenoic acid, lysoPC(18∶2), creatinine, lysoPC (16∶0) and lysoPE(22∶0/0∶0) in the FLP-treated group were completely reversed to levels in the control group which lacked CKD. Combined with biochemistry and histopathology results, the changes in serum metabolites indicate that the perturbations of phospholipids metabolism, energy metabolism and amino acid metabolism are related to adenine-induced CKD and to the interventions of FLP on all the three metabolic pathways. FLP may regulate the metabolism of these biomarkers, especially their efficient utilization within the context of CKD. Furthermore, these biomarkers might serve as characteristics to explain the mechanisms of FLP.
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urinary metabonomics study on the protective effects of ergosta 4 6 8 14 22 tetraen 3 one on chronic renal failure in rats using uplc q tof ms and a novel mse Data Collection Technique
Process Biochemistry, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Xuefeng Shen, Rui-chao LinAbstract:Abstract Ergosta-4,6,8(14),22-tetraen-3-one (ergone), isolated from the medicinal fungus Polyporus umbellatus, has been proven to prevent the progression of renal injury and the subsequent renal fibrosis. UPLC Q-TOF/MS was employed to investigate the metabonomic characteristics of adenine-induced chronic renal failure (CRF) and the proactive effects of ergone. The significant difference of the metabolic profiling was observed from ergone-treated group compared with the CRF model group during the 10-day and 20-day study periods by using the principal components analysis (PCA). The significant difference of the ergone-treated group in metabolic profiling was also observed between 10-day and 20-day study periods. The time-dependent tendency in ergone-treated group from day 10 to 20 was obtained, indicating the time-dependent recovery effect of ergone on CRF rats. Some significantly changed metabolites like creatinine, proline, adrenosterone, taurine, creatine, phenylalanine, ornithine, dopamine, kynurenine, kynurenic acid and 3-O-methyldopa have been identified during the 20-day study period. These biochemical changes are related to the disturbance in energy metabolism and amino acid metabolism, which are helpful to further understand the CRF and the therapeutic mechanism of ergone. This work suggests that this metabonomic approach could be used as a potentially powerful tool to investigate the biochemical changes of certain physiopathological conditions, such as metabolic syndrome, as an early diagnostic measure.
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application of faecal metabonomics on an experimental model of tubulointerstitial fibrosis by ultra performance liquid chromatography high sensitivity mass spectrometry with ms e Data Collection Technique
Biomarkers, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Rui-chao LinAbstract:Chronic renal failure (CRF) is a major challenge for the public healthcare problem. A novel UPLC Q-TOF/MS method with MSE Data Collection mode was developed as a very effective biochemical analytical tool for precise identification of important biomarkers in the adenine-induced CRF rats. Nine endogenous metabolites were identified by using metabonomic method combined with multivariate Data analysis, the accurate mass, isotopic pattern, MSE fragments information and MassLynx i-FIT algorithm. The identified metabolites indicated the perturbations of bile acid and phospholipid metabolism are related to CRF rats. This work shows that metabonomics method is a valuable tool in CRF mechanism study.
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Ultra performance liquid chromatography coupled with electrospray and atmospheric pressure chemical ionization (ESCi)-quadrupole time-of-flight mass spectrometry with novel mass spectrometry(Elevated Energy) (MS(E)) Data Collection Technique: determi
Journal of separation science, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Rui-chao LinAbstract:Ergosta-4,6,8(14),22-tetraen-3-one (ergone) has been proved to have novel antitumor effects on HepG2 cells. The aim of this study was to investigate the pharmacokinetics, tissue distribution, and biliary excretion of ergone in rats following a single oral administration (5, 10, and 20 mg/kg). The levels of ergone in plasma, tissues, and bile were measured by ultra performance liquid chromatography coupled with electrospray and atmospheric pressure chemical ionization (ESCi)-quadrupole time-of-flight mass spectrometry with novel mass spectrometryElevated Energy (MSE) Data Collection Technique method. The results show ergone was distributed and eliminated from rat plasma and in non-linear pharmacokinetics from a dose range of 5–20 mg/kg. The ergone was found to distribute widely in the internal organs, with tissue concentrations in order of lungs, spleen, liver, intestine, kidneys, heart, stomach, parorchis, teasticles, and brain. At 12 h after dosing, the tissue concentrations in the organs were markedly decreased. The lungs, spleen, and liver were the dominant organs with high tissue concentrations that might be the primary sites for metabolism and elimination of ergone. Total recoveries of ergone within 24 h in bile were 34.14%.
Michael G Crooks - One of the best experts on this subject based on the ideXlab platform.
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Post-COVID-19 Symptom Burden: What is Long-COVID and How Should We Manage It?
Lung, 2021Co-Authors: Dominic Lee Sykes, Luke Holdsworth, Nadia Jawad, Pumali Gunasekera, Alyn H Morice, Michael G CrooksAbstract:The enduring impact of COVID-19 on patients has been examined in recent studies, leading to the description of Long-COVID. We report the lasting symptom burden of COVID-19 patients from the first wave of the pandemic. All patients with COVID-19 pneumonia discharged from a large teaching hospital trust were offered follow-up. We assessed symptom burden at follow-up using a standardised Data Collection Technique during virtual outpatient clinic appointments. Eighty-six percent of patients reported at least one residual symptom at follow-up. No patients had persistent radiographic abnormalities. The presence of symptoms at follow-up was not associated with the severity of the acute COVID-19 illness. Females were significantly more likely to report residual symptoms including anxiety (p = 0.001), fatigue (p = 0.004), and myalgia (p = 0.022). The presence of long-lasting symptoms is common in COVID-19 patients. We suggest that the phenomenon of Long-COVID may not be directly attributable to the effect of SARS-CoV-2, and believe the biopsychosocial effects of COVID-19 may play a greater role in its aetiology.
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post covid 19 symptom burden what is long covid and how should we manage it
Lung, 2021Co-Authors: Dominic Lee Sykes, Luke Holdsworth, Nadia Jawad, Pumali Gunasekera, Alyn H Morice, Michael G CrooksAbstract:The enduring impact of COVID-19 on patients has been examined in recent studies, leading to the description of Long-COVID. We report the lasting symptom burden of COVID-19 patients from the first wave of the pandemic. All patients with COVID-19 pneumonia discharged from a large teaching hospital trust were offered follow-up. We assessed symptom burden at follow-up using a standardised Data Collection Technique during virtual outpatient clinic appointments. Eighty-six percent of patients reported at least one residual symptom at follow-up. No patients had persistent radiographic abnormalities. The presence of symptoms at follow-up was not associated with the severity of the acute COVID-19 illness. Females were significantly more likely to report residual symptoms including anxiety (p = 0.001), fatigue (p = 0.004), and myalgia (p = 0.022). The presence of long-lasting symptoms is common in COVID-19 patients. We suggest that the phenomenon of Long-COVID may not be directly attributable to the effect of SARS-CoV-2, and believe the biopsychosocial effects of COVID-19 may play a greater role in its aetiology.
Xu Bai - One of the best experts on this subject based on the ideXlab platform.
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renal metabolic profiling of early renal injury and renoprotective effects of poria cocos epidermis using uplc q tof hsms mse
Journal of Pharmaceutical and Biomedical Analysis, 2013Co-Authors: Ying-yong Zhao, Yalong Feng, Ping Lei, Danqian Chen, Xu BaiAbstract:Poria cocos epidermis is one of ancient traditional Chinese medicines (TCMs), which is usually used for the treatment of chronic kidney disease (CKD) for thousands of years in China. A metabonomic approach based on ultra performance liquid chromatography coupled with quadrupole time-of-flight high-sensitivity mass spectrometry (UPLC Q-TOF/HSMS) and a mass spectrometry(Elevated Energy) (MS(E)) Data Collection Technique was developed to obtained a systematic view of the development and progression of CKD and biochemistry mechanism of therapeutic effects of P. cocos epidermis (Fu-Ling-Pi, FLP). By partial least squares-discriminate analysis, 19 metabolites were identified as potential biomarkers of CKD. Among the 19 biomarkers, 10 biomarkers including eicosapentaenoic acid, docosahexaenoic acid, lysoPC(20:4), lysoPC(18:2), lysoPC(15:0), lysoPE(20:0/0:0), indoxyl sulfate, hippuric acid, p-cresol sulfate and allantoin were reversed to the control level in FLP-treated groups. The study indicates that FLP treatment can ameliorate CKD by intervening in some dominating metabolic pathways, such as fatty acid metabolism, phospholipid metabolism, purine metabolism and tryptophan metabolism. This work was for the first time to investigate the FLP therapeutic effect based on metabonomics technology, which is a potentially powerful tool to study the TCMs.
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ultra performance liquid chromatography based metabonomic study of therapeutic effect of the surface layer of poria cocos on adenine induced chronic kidney disease provides new insight into anti fibrosis mechanism
PLOS ONE, 2013Co-Authors: Ying-yong Zhao, Xu Bai, Rui-chao Lin, Yalong Feng, Xiaojie Tan, Qibing MeiAbstract:The surface layer of Poria cocos (Fu-Ling-Pi, FLP) is commonly used in traditional Chinese medicine and its diuretic effect was confirmed in rat. Ultra performance liquid chromatography/quadrupole time-of-flight high-sensitivity mass spectrometry and a novel mass spectrometryElevated Energy Data Collection Technique was employed to investigate metabonomic characteristics of chronic kidney disease (CKD) induced from adenine excess and the protective effects of FLP. Multiple metabolites are detected in the CKD and are correlated with progressive renal injury. Among these biomarkers, lysoPC(18∶0), tetracosahexaenoic acid, lysoPC(18∶2), creatinine, lysoPC (16∶0) and lysoPE(22∶0/0∶0) in the FLP-treated group were completely reversed to levels in the control group which lacked CKD. Combined with biochemistry and histopathology results, the changes in serum metabolites indicate that the perturbations of phospholipids metabolism, energy metabolism and amino acid metabolism are related to adenine-induced CKD and to the interventions of FLP on all the three metabolic pathways. FLP may regulate the metabolism of these biomarkers, especially their efficient utilization within the context of CKD. Furthermore, these biomarkers might serve as characteristics to explain the mechanisms of FLP.
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urinary metabonomics study on the protective effects of ergosta 4 6 8 14 22 tetraen 3 one on chronic renal failure in rats using uplc q tof ms and a novel mse Data Collection Technique
Process Biochemistry, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Xuefeng Shen, Rui-chao LinAbstract:Abstract Ergosta-4,6,8(14),22-tetraen-3-one (ergone), isolated from the medicinal fungus Polyporus umbellatus, has been proven to prevent the progression of renal injury and the subsequent renal fibrosis. UPLC Q-TOF/MS was employed to investigate the metabonomic characteristics of adenine-induced chronic renal failure (CRF) and the proactive effects of ergone. The significant difference of the metabolic profiling was observed from ergone-treated group compared with the CRF model group during the 10-day and 20-day study periods by using the principal components analysis (PCA). The significant difference of the ergone-treated group in metabolic profiling was also observed between 10-day and 20-day study periods. The time-dependent tendency in ergone-treated group from day 10 to 20 was obtained, indicating the time-dependent recovery effect of ergone on CRF rats. Some significantly changed metabolites like creatinine, proline, adrenosterone, taurine, creatine, phenylalanine, ornithine, dopamine, kynurenine, kynurenic acid and 3-O-methyldopa have been identified during the 20-day study period. These biochemical changes are related to the disturbance in energy metabolism and amino acid metabolism, which are helpful to further understand the CRF and the therapeutic mechanism of ergone. This work suggests that this metabonomic approach could be used as a potentially powerful tool to investigate the biochemical changes of certain physiopathological conditions, such as metabolic syndrome, as an early diagnostic measure.
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application of faecal metabonomics on an experimental model of tubulointerstitial fibrosis by ultra performance liquid chromatography high sensitivity mass spectrometry with ms e Data Collection Technique
Biomarkers, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Rui-chao LinAbstract:Chronic renal failure (CRF) is a major challenge for the public healthcare problem. A novel UPLC Q-TOF/MS method with MSE Data Collection mode was developed as a very effective biochemical analytical tool for precise identification of important biomarkers in the adenine-induced CRF rats. Nine endogenous metabolites were identified by using metabonomic method combined with multivariate Data analysis, the accurate mass, isotopic pattern, MSE fragments information and MassLynx i-FIT algorithm. The identified metabolites indicated the perturbations of bile acid and phospholipid metabolism are related to CRF rats. This work shows that metabonomics method is a valuable tool in CRF mechanism study.
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Ultra performance liquid chromatography coupled with electrospray and atmospheric pressure chemical ionization (ESCi)-quadrupole time-of-flight mass spectrometry with novel mass spectrometry(Elevated Energy) (MS(E)) Data Collection Technique: determi
Journal of separation science, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Rui-chao LinAbstract:Ergosta-4,6,8(14),22-tetraen-3-one (ergone) has been proved to have novel antitumor effects on HepG2 cells. The aim of this study was to investigate the pharmacokinetics, tissue distribution, and biliary excretion of ergone in rats following a single oral administration (5, 10, and 20 mg/kg). The levels of ergone in plasma, tissues, and bile were measured by ultra performance liquid chromatography coupled with electrospray and atmospheric pressure chemical ionization (ESCi)-quadrupole time-of-flight mass spectrometry with novel mass spectrometryElevated Energy (MSE) Data Collection Technique method. The results show ergone was distributed and eliminated from rat plasma and in non-linear pharmacokinetics from a dose range of 5–20 mg/kg. The ergone was found to distribute widely in the internal organs, with tissue concentrations in order of lungs, spleen, liver, intestine, kidneys, heart, stomach, parorchis, teasticles, and brain. At 12 h after dosing, the tissue concentrations in the organs were markedly decreased. The lungs, spleen, and liver were the dominant organs with high tissue concentrations that might be the primary sites for metabolism and elimination of ergone. Total recoveries of ergone within 24 h in bile were 34.14%.
Rui-chao Lin - One of the best experts on this subject based on the ideXlab platform.
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ultra performance liquid chromatography based metabonomic study of therapeutic effect of the surface layer of poria cocos on adenine induced chronic kidney disease provides new insight into anti fibrosis mechanism
PLOS ONE, 2013Co-Authors: Ying-yong Zhao, Xu Bai, Rui-chao Lin, Yalong Feng, Xiaojie Tan, Qibing MeiAbstract:The surface layer of Poria cocos (Fu-Ling-Pi, FLP) is commonly used in traditional Chinese medicine and its diuretic effect was confirmed in rat. Ultra performance liquid chromatography/quadrupole time-of-flight high-sensitivity mass spectrometry and a novel mass spectrometryElevated Energy Data Collection Technique was employed to investigate metabonomic characteristics of chronic kidney disease (CKD) induced from adenine excess and the protective effects of FLP. Multiple metabolites are detected in the CKD and are correlated with progressive renal injury. Among these biomarkers, lysoPC(18∶0), tetracosahexaenoic acid, lysoPC(18∶2), creatinine, lysoPC (16∶0) and lysoPE(22∶0/0∶0) in the FLP-treated group were completely reversed to levels in the control group which lacked CKD. Combined with biochemistry and histopathology results, the changes in serum metabolites indicate that the perturbations of phospholipids metabolism, energy metabolism and amino acid metabolism are related to adenine-induced CKD and to the interventions of FLP on all the three metabolic pathways. FLP may regulate the metabolism of these biomarkers, especially their efficient utilization within the context of CKD. Furthermore, these biomarkers might serve as characteristics to explain the mechanisms of FLP.
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urinary metabonomics study on the protective effects of ergosta 4 6 8 14 22 tetraen 3 one on chronic renal failure in rats using uplc q tof ms and a novel mse Data Collection Technique
Process Biochemistry, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Xuefeng Shen, Rui-chao LinAbstract:Abstract Ergosta-4,6,8(14),22-tetraen-3-one (ergone), isolated from the medicinal fungus Polyporus umbellatus, has been proven to prevent the progression of renal injury and the subsequent renal fibrosis. UPLC Q-TOF/MS was employed to investigate the metabonomic characteristics of adenine-induced chronic renal failure (CRF) and the proactive effects of ergone. The significant difference of the metabolic profiling was observed from ergone-treated group compared with the CRF model group during the 10-day and 20-day study periods by using the principal components analysis (PCA). The significant difference of the ergone-treated group in metabolic profiling was also observed between 10-day and 20-day study periods. The time-dependent tendency in ergone-treated group from day 10 to 20 was obtained, indicating the time-dependent recovery effect of ergone on CRF rats. Some significantly changed metabolites like creatinine, proline, adrenosterone, taurine, creatine, phenylalanine, ornithine, dopamine, kynurenine, kynurenic acid and 3-O-methyldopa have been identified during the 20-day study period. These biochemical changes are related to the disturbance in energy metabolism and amino acid metabolism, which are helpful to further understand the CRF and the therapeutic mechanism of ergone. This work suggests that this metabonomic approach could be used as a potentially powerful tool to investigate the biochemical changes of certain physiopathological conditions, such as metabolic syndrome, as an early diagnostic measure.
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application of faecal metabonomics on an experimental model of tubulointerstitial fibrosis by ultra performance liquid chromatography high sensitivity mass spectrometry with ms e Data Collection Technique
Biomarkers, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Rui-chao LinAbstract:Chronic renal failure (CRF) is a major challenge for the public healthcare problem. A novel UPLC Q-TOF/MS method with MSE Data Collection mode was developed as a very effective biochemical analytical tool for precise identification of important biomarkers in the adenine-induced CRF rats. Nine endogenous metabolites were identified by using metabonomic method combined with multivariate Data analysis, the accurate mass, isotopic pattern, MSE fragments information and MassLynx i-FIT algorithm. The identified metabolites indicated the perturbations of bile acid and phospholipid metabolism are related to CRF rats. This work shows that metabonomics method is a valuable tool in CRF mechanism study.
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Ultra performance liquid chromatography coupled with electrospray and atmospheric pressure chemical ionization (ESCi)-quadrupole time-of-flight mass spectrometry with novel mass spectrometry(Elevated Energy) (MS(E)) Data Collection Technique: determi
Journal of separation science, 2012Co-Authors: Ying-yong Zhao, Xian-long Cheng, Feng Wei, Xu Bai, Rui-chao LinAbstract:Ergosta-4,6,8(14),22-tetraen-3-one (ergone) has been proved to have novel antitumor effects on HepG2 cells. The aim of this study was to investigate the pharmacokinetics, tissue distribution, and biliary excretion of ergone in rats following a single oral administration (5, 10, and 20 mg/kg). The levels of ergone in plasma, tissues, and bile were measured by ultra performance liquid chromatography coupled with electrospray and atmospheric pressure chemical ionization (ESCi)-quadrupole time-of-flight mass spectrometry with novel mass spectrometryElevated Energy (MSE) Data Collection Technique method. The results show ergone was distributed and eliminated from rat plasma and in non-linear pharmacokinetics from a dose range of 5–20 mg/kg. The ergone was found to distribute widely in the internal organs, with tissue concentrations in order of lungs, spleen, liver, intestine, kidneys, heart, stomach, parorchis, teasticles, and brain. At 12 h after dosing, the tissue concentrations in the organs were markedly decreased. The lungs, spleen, and liver were the dominant organs with high tissue concentrations that might be the primary sites for metabolism and elimination of ergone. Total recoveries of ergone within 24 h in bile were 34.14%.
