The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Beata Hasiowjaroszewska - One of the best experts on this subject based on the ideXlab platform.
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molecular evolution of pepino mosaic virus during long term passaging in different hosts and its impact on virus virulence
Annals of Applied Biology, 2015Co-Authors: Julia Minicka, N Rymelska, Santiago F Elena, Anna Czerwoniec, Beata HasiowjaroszewskaAbstract:In this study the effect of host changes and multiple passages on Pepino mosaic virus (PepMV) evolution was analysed. A population of a mild isolate of PepMV was used to generate five independent evolution lineages on three tomato cultivars, which differ in rate of appearance of symptoms and their severity during viral infection (Beta Lux, Moneymaker and Malinowy O zarowski) and on Datura Inoxia. Twenty serial passages were performed over a period of 217–220 days. Symptom severity was monitored along the entire experiment. After the last series of passages total RNAs from each lineage and host were isolated and the triple gene block 3 (TGB3) and coat protein (CP) were amplified, cloned and 10 clones for each gene sequenced. Among the 400 clones for both genes, 143 individual mutations (61 synonymous and 82 nonsynonymous) were identified, with the largest number of nonsynonymous mutations being observed for the tomato cultivars Malinowy O zarowski and Beta Lux. In two of the lineages evolving in the most susceptible variety of tomato (Beta Lux) necrotic changes in leaf blades appeared after 17 passages, leading to death of the plants. In these two lineages the mutation responsible for necrotic symptoms was K67E in TGB3. The appearance of this convergent mutation in independently evolving lineages may suggest that selection in this experimental set up favours more aggressive PepMV variants. We found a positive association between the severity of symptoms and the amount of genetic variability contained on viral populations. Indeed, the severity of symptoms turned out to be a good predictor for several indices of molecular variability. In addition, mapping all observed mutations in CP and TGB3 protein structures revealed that most were located on the surface, indicating a possible implication in viral–viral or viral–host interactions.
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single mutation converts mild pathotype of the pepino mosaic virus into necrotic one
Virus Research, 2011Co-Authors: Beata Hasiowjaroszewska, Paulina Jackowiak, Marek Figlerowicz, Natasza Borodynko, Henryk PospiesznyAbstract:Abstract Pepino mosaic virus (PepMV) is a member of the Flexiviridae family, genus Potexvirus . PepMV isolates from the same genotype differ in the symptoms they induce in Datura Inoxia and Solanum lycopersicum . Necrotic isolates cause necrosis on these plants whereas mild isolates are very often asymptomatic or induce mild mosaic. Sequence analysis of the genomes of mild (PepMV-P22) and necrotic (PepMV-P19) strains revealed that they differ by several nonsynonymous mutations. Eleven mutations are located in the RdRp coding region and one mutation is placed in the TGB3 coding region. To investigate whether these mutations affect the symptom induction, both PepMV-P22 and PepMV-P19 were mutagenized. As a result we found that the genetic determinant responsible for necrosis induction was amino acid 67 of TGB3. This showed that one point mutation is sufficient to alter the virus from mild to aggressive in tomato and D. Inoxia .
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new necrotic isolates of pepino mosaic virus representing the ch2 genotype
Journal of Phytopathology, 2009Co-Authors: Beata Hasiowjaroszewska, Henryk Pospieszny, Natasza BorodynkoAbstract:New necrotic isolates of Pepino mosaic virus (PepMV) were found in 2007 infecting greenhouse tomato plants in Poland. The isolates differ from previously identified PepMV isolates in host range and symptomatology. They induce severe necrosis on tomato plants (Solanum lycopersicum) and local necrotic lesions on Datura Inoxia. Phylogenetic analysis, based on three distinct regions, triple gene block 1, the coat protein gene and a part of polymerase gene, revealed that the new necrotic isolates share high nucleotide sequence identity with isolates of the Ch2 genotype. This is the first report describing a necrotic type of PepMV of the Ch2 genotype.
Babu Gajendran - One of the best experts on this subject based on the ideXlab platform.
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a novel phytosterol isolated from Datura Inoxia rInoxiab is a potential cure colon cancer agent by targeting bax bcl2 pathway
Bioorganic & Medicinal Chemistry, 2020Co-Authors: Prabhu Durai, Krishnapriya Madhu Varier, Babu Gajendran, Arulvasu ChinnasamyAbstract:Abstract Plant sterols have been widely used as chemotherapeutic agents for colorectal cancer for years together. In this study, a novel phytosterol was isolated and characterized from the leaf extract of a medicinal plant, Datura Inoxia and was coined as RInoxiaB (RB). This phytosterol was observed to have antiproliferative activity against human colon adenocarcinoma cells, HCT 15. The cell viability assay revealed the IC50 value of the RB as 4 µM. Moreover, RB treated cells showed prominent morphological changes dose dependently and progressively increased the number of dead cells. Additionally, results of the comet, flow cytometry, and cell cycle analysis revealed that the majority of cells were arrested in their S and G2/M phase by blocking the mitotic spindle formation. The western blot analysis (Bcl-2, BAX, Cytochrome C, Caspases 9 & 3) clearly indicated that RB has the ability to induce apoptosis by significantly upregulating (P
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green synthesis of silver nanoparticle from Datura Inoxia flower extract and its cytotoxic activity
Journal of Bionanoscience, 2019Co-Authors: Radhakrishnan Nagarathnam, Prabhu Durai, Saravanan Rajendran, Krishnapriya Madhu Varier, Yanmei Li, Babu Gajendran, Arulvasu ChinnasamyAbstract:The silver nanoparticles (AgNPs) were synthesized biologically through a green synthesis method using the flower extract of Datura Inoxia, a well-known medicinal plant for its antispasmodic, pacifying, pain relief, and for respiratory ailments. The synthesized AgNPs were structurally characterized and the cytotoxic efficiency of the AgNPs on human breast cancer cell line (MCF7) was appraised by cell viability assay, followed by cytomorphological analysis. However, apoptosis initiated by the AgNPs administration was assessed through nuclear fragmentation assay, cell cycle arrest, and comet assay. The synthesized AgNPs inhibited the propagation of cells at an IC50 concentration of 20 μg/mL after 24 h incubation. The AgNPs promoted apoptosis through DNA damage. Thus, this study reveals that the green synthesis is an ecofriendly method for production of AgNPs from Datura Inoxia flower extract which provided a powerful anti-proliferative action on MCF7 cell line, suggesting them as a novel chemotherapeutic agent against human breast cancer.
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biosynthesis and characterization of silver nanoparticles from Datura Inoxia and its apoptotic effect on human breast cancer cell line mcf7
Materials Letters, 2014Co-Authors: Babu Gajendran, Prabhu Durai, Arulvasu Chinnasamy, Jegadeesh Raman, Manikandan RamarAbstract:Abstract The present study demonstrates the efficacy of Datura Inoxia for biosynthesis of silver nanoparticles (AgNPs). The aqueous extract obtained from the leaves of D. Inoxia was mixed with AgNO 3 and incubated for the synthesis of AgNPs. The synthesized nanoparticles were characterized by UV–visible, Field Emission Scanning Electron Microscope (FESEM), Energy-Dispersive X-ray Spectroscopy (EDX), Transmission Electron Microscope (TEM), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The toxicity of AgNPs was evaluated using cell viability, nuclear fragmentation and cell cycle. Our results showed that biosynthesized AgNPs inhibited proliferation of human breast cancer cell line MCF7 with an IC 50 of 20 µg/ml at 24 h incubation. These results suggest that AgNPs may exert its antiproliferative effect on MCF7 cell line by suppressing its growth, arresting the cell cycle phases, reducing DNA synthesis and inducing apoptosis.
Henryk Pospieszny - One of the best experts on this subject based on the ideXlab platform.
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single mutation converts mild pathotype of the pepino mosaic virus into necrotic one
Virus Research, 2011Co-Authors: Beata Hasiowjaroszewska, Paulina Jackowiak, Marek Figlerowicz, Natasza Borodynko, Henryk PospiesznyAbstract:Abstract Pepino mosaic virus (PepMV) is a member of the Flexiviridae family, genus Potexvirus . PepMV isolates from the same genotype differ in the symptoms they induce in Datura Inoxia and Solanum lycopersicum . Necrotic isolates cause necrosis on these plants whereas mild isolates are very often asymptomatic or induce mild mosaic. Sequence analysis of the genomes of mild (PepMV-P22) and necrotic (PepMV-P19) strains revealed that they differ by several nonsynonymous mutations. Eleven mutations are located in the RdRp coding region and one mutation is placed in the TGB3 coding region. To investigate whether these mutations affect the symptom induction, both PepMV-P22 and PepMV-P19 were mutagenized. As a result we found that the genetic determinant responsible for necrosis induction was amino acid 67 of TGB3. This showed that one point mutation is sufficient to alter the virus from mild to aggressive in tomato and D. Inoxia .
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new necrotic isolates of pepino mosaic virus representing the ch2 genotype
Journal of Phytopathology, 2009Co-Authors: Beata Hasiowjaroszewska, Henryk Pospieszny, Natasza BorodynkoAbstract:New necrotic isolates of Pepino mosaic virus (PepMV) were found in 2007 infecting greenhouse tomato plants in Poland. The isolates differ from previously identified PepMV isolates in host range and symptomatology. They induce severe necrosis on tomato plants (Solanum lycopersicum) and local necrotic lesions on Datura Inoxia. Phylogenetic analysis, based on three distinct regions, triple gene block 1, the coat protein gene and a part of polymerase gene, revealed that the new necrotic isolates share high nucleotide sequence identity with isolates of the Ch2 genotype. This is the first report describing a necrotic type of PepMV of the Ch2 genotype.
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new polish isolate of pepino mosaic virus highly distinct from european tomato peruvian and us2 strains
Plant Disease, 2006Co-Authors: Henryk Pospieszny, Natasza BorodynkoAbstract:Pepino mosaic virus (PepMV, genus Potexvirus) was first described on pepino (Solanum muricatum) in Peru during 1980. Since 1999, the virus was reported in several European countries and in North and South America as an agent of viral disease of tomato crops. In Poland in 2002, the PepMV-SW isolate that was genetically similar to European isolates (approximately 99% identity) was identified (3). In November 2005, in the western part of the Wielkopolska Region, a virus with flexuous filamentous particles approximately 500 nm long was isolated from tomato fruits exhibiting symptoms of discoloration. Crude sap from Nicotiana benthamiana leaves was used for mechanical inoculation of indicator plants. The virus caused symptoms on N. benthamiana, N. clevelandii, Datura Inoxia, and Lycopersicon esculentum. Symptomless infection on N. tabacum cv. Xanthi nc, N. tabacum cv. White Burley, and N. debneyi was confirmed by back-inoculation on N. benthamiana. The virus did not infect N. glutinosa, Physalis floridana, Pet...
Natasza Borodynko - One of the best experts on this subject based on the ideXlab platform.
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single mutation converts mild pathotype of the pepino mosaic virus into necrotic one
Virus Research, 2011Co-Authors: Beata Hasiowjaroszewska, Paulina Jackowiak, Marek Figlerowicz, Natasza Borodynko, Henryk PospiesznyAbstract:Abstract Pepino mosaic virus (PepMV) is a member of the Flexiviridae family, genus Potexvirus . PepMV isolates from the same genotype differ in the symptoms they induce in Datura Inoxia and Solanum lycopersicum . Necrotic isolates cause necrosis on these plants whereas mild isolates are very often asymptomatic or induce mild mosaic. Sequence analysis of the genomes of mild (PepMV-P22) and necrotic (PepMV-P19) strains revealed that they differ by several nonsynonymous mutations. Eleven mutations are located in the RdRp coding region and one mutation is placed in the TGB3 coding region. To investigate whether these mutations affect the symptom induction, both PepMV-P22 and PepMV-P19 were mutagenized. As a result we found that the genetic determinant responsible for necrosis induction was amino acid 67 of TGB3. This showed that one point mutation is sufficient to alter the virus from mild to aggressive in tomato and D. Inoxia .
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new necrotic isolates of pepino mosaic virus representing the ch2 genotype
Journal of Phytopathology, 2009Co-Authors: Beata Hasiowjaroszewska, Henryk Pospieszny, Natasza BorodynkoAbstract:New necrotic isolates of Pepino mosaic virus (PepMV) were found in 2007 infecting greenhouse tomato plants in Poland. The isolates differ from previously identified PepMV isolates in host range and symptomatology. They induce severe necrosis on tomato plants (Solanum lycopersicum) and local necrotic lesions on Datura Inoxia. Phylogenetic analysis, based on three distinct regions, triple gene block 1, the coat protein gene and a part of polymerase gene, revealed that the new necrotic isolates share high nucleotide sequence identity with isolates of the Ch2 genotype. This is the first report describing a necrotic type of PepMV of the Ch2 genotype.
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new polish isolate of pepino mosaic virus highly distinct from european tomato peruvian and us2 strains
Plant Disease, 2006Co-Authors: Henryk Pospieszny, Natasza BorodynkoAbstract:Pepino mosaic virus (PepMV, genus Potexvirus) was first described on pepino (Solanum muricatum) in Peru during 1980. Since 1999, the virus was reported in several European countries and in North and South America as an agent of viral disease of tomato crops. In Poland in 2002, the PepMV-SW isolate that was genetically similar to European isolates (approximately 99% identity) was identified (3). In November 2005, in the western part of the Wielkopolska Region, a virus with flexuous filamentous particles approximately 500 nm long was isolated from tomato fruits exhibiting symptoms of discoloration. Crude sap from Nicotiana benthamiana leaves was used for mechanical inoculation of indicator plants. The virus caused symptoms on N. benthamiana, N. clevelandii, Datura Inoxia, and Lycopersicon esculentum. Symptomless infection on N. tabacum cv. Xanthi nc, N. tabacum cv. White Burley, and N. debneyi was confirmed by back-inoculation on N. benthamiana. The virus did not infect N. glutinosa, Physalis floridana, Pet...
Arulvasu Chinnasamy - One of the best experts on this subject based on the ideXlab platform.
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a novel phytosterol isolated from Datura Inoxia rInoxiab is a potential cure colon cancer agent by targeting bax bcl2 pathway
Bioorganic & Medicinal Chemistry, 2020Co-Authors: Prabhu Durai, Krishnapriya Madhu Varier, Babu Gajendran, Arulvasu ChinnasamyAbstract:Abstract Plant sterols have been widely used as chemotherapeutic agents for colorectal cancer for years together. In this study, a novel phytosterol was isolated and characterized from the leaf extract of a medicinal plant, Datura Inoxia and was coined as RInoxiaB (RB). This phytosterol was observed to have antiproliferative activity against human colon adenocarcinoma cells, HCT 15. The cell viability assay revealed the IC50 value of the RB as 4 µM. Moreover, RB treated cells showed prominent morphological changes dose dependently and progressively increased the number of dead cells. Additionally, results of the comet, flow cytometry, and cell cycle analysis revealed that the majority of cells were arrested in their S and G2/M phase by blocking the mitotic spindle formation. The western blot analysis (Bcl-2, BAX, Cytochrome C, Caspases 9 & 3) clearly indicated that RB has the ability to induce apoptosis by significantly upregulating (P
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green synthesis of silver nanoparticle from Datura Inoxia flower extract and its cytotoxic activity
Journal of Bionanoscience, 2019Co-Authors: Radhakrishnan Nagarathnam, Prabhu Durai, Saravanan Rajendran, Krishnapriya Madhu Varier, Yanmei Li, Babu Gajendran, Arulvasu ChinnasamyAbstract:The silver nanoparticles (AgNPs) were synthesized biologically through a green synthesis method using the flower extract of Datura Inoxia, a well-known medicinal plant for its antispasmodic, pacifying, pain relief, and for respiratory ailments. The synthesized AgNPs were structurally characterized and the cytotoxic efficiency of the AgNPs on human breast cancer cell line (MCF7) was appraised by cell viability assay, followed by cytomorphological analysis. However, apoptosis initiated by the AgNPs administration was assessed through nuclear fragmentation assay, cell cycle arrest, and comet assay. The synthesized AgNPs inhibited the propagation of cells at an IC50 concentration of 20 μg/mL after 24 h incubation. The AgNPs promoted apoptosis through DNA damage. Thus, this study reveals that the green synthesis is an ecofriendly method for production of AgNPs from Datura Inoxia flower extract which provided a powerful anti-proliferative action on MCF7 cell line, suggesting them as a novel chemotherapeutic agent against human breast cancer.
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biosynthesis and characterization of silver nanoparticles from Datura Inoxia and its apoptotic effect on human breast cancer cell line mcf7
Materials Letters, 2014Co-Authors: Babu Gajendran, Prabhu Durai, Arulvasu Chinnasamy, Jegadeesh Raman, Manikandan RamarAbstract:Abstract The present study demonstrates the efficacy of Datura Inoxia for biosynthesis of silver nanoparticles (AgNPs). The aqueous extract obtained from the leaves of D. Inoxia was mixed with AgNO 3 and incubated for the synthesis of AgNPs. The synthesized nanoparticles were characterized by UV–visible, Field Emission Scanning Electron Microscope (FESEM), Energy-Dispersive X-ray Spectroscopy (EDX), Transmission Electron Microscope (TEM), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The toxicity of AgNPs was evaluated using cell viability, nuclear fragmentation and cell cycle. Our results showed that biosynthesized AgNPs inhibited proliferation of human breast cancer cell line MCF7 with an IC 50 of 20 µg/ml at 24 h incubation. These results suggest that AgNPs may exert its antiproliferative effect on MCF7 cell line by suppressing its growth, arresting the cell cycle phases, reducing DNA synthesis and inducing apoptosis.
