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Peter Tschoppe - One of the best experts on this subject based on the ideXlab platform.
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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyer-lueckel, Sebastian Paris, Richard Johannes WierichsAbstract:ObjectiveThe aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany.MethodsIn each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (Δ Z _B). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F^− [pH = 6.04] (NaF-gel_1), 12.500 ppm F^− [pH = 7.34] (NaF-gel_2), 12.500 ppm F^− [pH = 5.82] (AmF-gel), 1450 ppm F^− [pH = 7.35] (KF-gel), and 5000 ppm F^− [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔ Z _E1/ΔΔ Z _E2) were calculated between values before and after pH cycling.ResultsMean (95%CI) Δ Z _B was 3851 (3762;3939) vol% × μm. Except for NaF-gel_2 and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel_2 induced a significant gain in mineral content ( p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔ Z _E1 and for ΔΔ Z _E2 ( p
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effect of naf amf kf gels and naf toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyerlueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p < 0.05, Bonferroni post hoc test). However, only NaF-gel and NaF-TP induced remineralization. CONCLUSION Under the in vitro conditions chosen, all fluoride agents could significantly hamper the adverse effects of a Demineralizing saliva substitute. CLINICAL SIGNIFICANCE In combination with a Demineralizing saliva substitute, slight mineral gain was only observed for neutral NaF-gel and 5000 ppm F toothpaste.
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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro.
Clinical Oral Investigations, 2018Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyer-lueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p
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Effects of regular and highly fluoridated toothpastes in combination with saliva substitutes on artificial enamel caries lesions differing in mineral content.
Archives of Oral Biology, 2012Co-Authors: Peter Tschoppe, Hendrik Meyer-lueckelAbstract:OBJECTIVE: For patients with hyposalivation fluorides are supportive to prevent caries lesions. Remineralization of subsurface lesions might be improved by toothpastes containing 5000 μgF(-)/g compared with those having 1400 μgF(-)/g. This could be influenced by the degree of baseline mineralization. Therefore, this in vitro study evaluated the effects of fluoride toothpastes differing in fluoride concentration in combination with de- and remineralizing saliva substitutes using two lesion types. DESIGN: Specimens with shallow (SL; ΔZ (SD): 1915 (543) vol% × μm) or deep lesions (DL; 5804 (427) vol% × μm) were either stored in mineral water [saturation with respect to octacalcium phosphate (S(OCP)): 0.5], Demineralizing experimental (Exp, S(OCP): 0.3), Demineralizing commercial (Glandosane, S(OCP): 0.3), or remineralizing saliva substitute (modified Saliva natura; S(OCP): 1.9) for five weeks (37 °C). Either one of three brushing procedures was performed additionally three times daily: no brushing, Elmex anticaries toothpaste (E; 1400 μgF(-)/g), Duraphat toothpaste (D; 5000 μgF(-)/g). Mineral parameters before and after storage were evaluated using microradiographs. RESULTS: Storage in Exp as well as Glandosane induced a significant demineralization (p < 0.05; relatively more pronounced in SL than DL). Additional brushing in particular with D reduced these effects. Storage alone in modified Saliva natura remineralized specimens (p < 0.05). CONCLUSIONS: Under the in vitro conditions chosen shallow lesions seem to be more susceptible for demineralization compared with deeper ones when stored in an undersaturated (with respect to OCP) saliva substitute. The highly fluoridated toothpaste seemed to be more beneficial than a regular one.
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Design of a randomized controlled double-blind crossover clinical trial to assess the effects of saliva substitutes on bovine enamel and dentin in situ
BMC Oral Health, 2011Co-Authors: Peter Tschoppe, Olivia Wolf, Peter Martus, Michael Eichhorn, Andrej M KielbassaAbstract:Background: Hyposalivation is caused by various syndromes, diabetes, drugs, inflammation, infection, or radiotherapy of the salivary glands. Patients with hyposalivation often show an increased caries incidence. Moreover, hyposalivation is frequently accompanied by oral discomfort and impaired oral functions, and saliva substitutes are widely used to alleviate oral symptoms. However, preference of saliva substitutes due to taste, handling, and relief of oral symptoms has been discussed controversially. Some of the marketed products have shown Demineralizing effects on dental hard tissues in vitro. This Demineralizing potential is attributed to the undersaturation with respect to calcium phosphates. Therefore, it is important to modify the mineralizing potential of saliva substitutes to prevent carious lesions. Thus, the aim of the present study was to evaluate the effects of a possible remineralizing saliva substitute (SN; modified Saliva natura) compared to a Demineralizing one (G; Glandosane) on mineral parameters of sound bovine dentin and enamel as well as on artificially demineralized enamel specimens in situ. Moreover, oral well-being after use of each saliva substitute was recorded. Methods/Design: Using a randomized, double-blind, crossover, phase II/III in situ trial, volunteers with hyposalivation utilize removable dentures containing bovine specimens during the experimental period. The volunteers are divided into two groups, and are required to apply both saliva substitutes for seven weeks each. After both test periods, differences in mineral loss and lesion depth between values before and after exposure are evaluated based on microradiographs. The oral well-being of the volunteers before and after therapy is determined using questionnaires. With respect to the microradiographic analysis, equal mineral losses and lesion depths of enamel and dentin specimens during treatment with SN and G, and no differences in patients’ experienced oral comfort after SN compared to G usage are expected (H0). Discussion: Up to now, 14 patients have been included in the study, and no reasons for early termination of the trial have been identified. The design seems suitable for determining the effects of saliva substitutes on dental hard tissues in situ, and should provide detailed information on the oral well-being after use of different saliva substitutes in patients with hyposalivation.
Richard Johannes Wierichs - One of the best experts on this subject based on the ideXlab platform.
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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyer-lueckel, Sebastian Paris, Richard Johannes WierichsAbstract:ObjectiveThe aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany.MethodsIn each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (Δ Z _B). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F^− [pH = 6.04] (NaF-gel_1), 12.500 ppm F^− [pH = 7.34] (NaF-gel_2), 12.500 ppm F^− [pH = 5.82] (AmF-gel), 1450 ppm F^− [pH = 7.35] (KF-gel), and 5000 ppm F^− [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔ Z _E1/ΔΔ Z _E2) were calculated between values before and after pH cycling.ResultsMean (95%CI) Δ Z _B was 3851 (3762;3939) vol% × μm. Except for NaF-gel_2 and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel_2 induced a significant gain in mineral content ( p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔ Z _E1 and for ΔΔ Z _E2 ( p
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effect of naf amf kf gels and naf toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyerlueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p < 0.05, Bonferroni post hoc test). However, only NaF-gel and NaF-TP induced remineralization. CONCLUSION Under the in vitro conditions chosen, all fluoride agents could significantly hamper the adverse effects of a Demineralizing saliva substitute. CLINICAL SIGNIFICANCE In combination with a Demineralizing saliva substitute, slight mineral gain was only observed for neutral NaF-gel and 5000 ppm F toothpaste.
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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro.
Clinical Oral Investigations, 2018Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyer-lueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p
Hendrik Meyerlueckel - One of the best experts on this subject based on the ideXlab platform.
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effect of naf amf kf gels and naf toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyerlueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p < 0.05, Bonferroni post hoc test). However, only NaF-gel and NaF-TP induced remineralization. CONCLUSION Under the in vitro conditions chosen, all fluoride agents could significantly hamper the adverse effects of a Demineralizing saliva substitute. CLINICAL SIGNIFICANCE In combination with a Demineralizing saliva substitute, slight mineral gain was only observed for neutral NaF-gel and 5000 ppm F toothpaste.
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in vitro analysis of an new saliva substitute saliva natura on enamel and dentin
Laryngo-rhino-otologie, 2007Co-Authors: Peter Tschoppe, Rod Toll, Hendrik Meyerlueckel, Andrej M KielbassaAbstract:BACKGROUND: Hyposalivation is an important chronic side effect of radiotherapy in the head and neck area, and patients often alleviate their symptoms using saliva substitutes. The aim of this study was to evaluate the effects of two commercially available saliva substitutes (Saliva natura and Glandosane) on the mineral loss of bovine enamel and dentin in vitro. An aqueous remineralization solution served as control. METHODS: Each 45 bovine enamel and dentin specimens were prepared. Prior to (control of sound dentin) and after (control of demineralized dentin) demineralization (37 degrees C; enamel: pH 4.95; 14 d; dentin: pH 5.0; 7 d) one third of each specimens surface was covered with nail varnish. Subsequently, the specimens (n = 15) were exposed to Glandosane and Saliva natura as well as a remineralization solution for 14 days (37 degrees C). Specimens were examined using transversal microradiography. RESULTS: Compared to Saliva natura and the reminerlization solution, Glandosane induced both significantly increased mineral losses as well as lesion depths of the enamel specimens (p < 0.05; ANOVA, Bonferroni). After exposure of the dentin specimens to Saliva natura a significantly increased mineral loss could be observed (p < 0.001), whereas no differences in mineral loss could be observed for the enamel specimens (p = 0.078; t-test). CONCLUSIONS: Within the limitations of an in vitro study it can be concluded that Glandosane revealed a Demineralizing potential on bovine enamel as well as on dentin and should not be recommended for dentate patients. Since Saliva natura has a Demineralizing effect on dentin, a further improvement regarding the remineralizing capacity would be desirable.
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progression of sealed initial bovine enamel lesions under Demineralizing conditions in vitro
Caries Research, 2006Co-Authors: Sebastian Paris, Hendrik Meyerlueckel, Jan Mueller, M Hummel, Andrej M KielbassaAbstract:The use of sealants for the infiltration of proximal enamel lesions could be a promising alternative to the common strategies of remineralization and operative treatment. The aim of the present study was to compare the progression of sealed initial enamel lesions after exposure to a Demineralizing solution in vitro. In each of 54 bovine enamel specimens three subsurface lesions were created. Two of the lesions were etched with phosphoric acid and sealed with either a fissure sealant or with various adhesives (1–5) for 15 s or 30 s, respectively, whereas one lesion remained as the untreated control. Subsequently, half of each specimen was covered with nail varnish (baseline) and the other half was reexposed to a Demineralizing solution for 14 days (experimental). The specimens were cut perpendicularly to the surface, infiltrated with a low-viscosity fluorescent resin and observed with a confocal laser scanning microscope (CLSM). For lesions sealed with the fissure sealant and adhesives 1–3, the progression of lesion depth (0–31 µm) was significantly decreased (p
Sebastian Paris - One of the best experts on this subject based on the ideXlab platform.
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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyer-lueckel, Sebastian Paris, Richard Johannes WierichsAbstract:ObjectiveThe aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany.MethodsIn each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (Δ Z _B). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F^− [pH = 6.04] (NaF-gel_1), 12.500 ppm F^− [pH = 7.34] (NaF-gel_2), 12.500 ppm F^− [pH = 5.82] (AmF-gel), 1450 ppm F^− [pH = 7.35] (KF-gel), and 5000 ppm F^− [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔ Z _E1/ΔΔ Z _E2) were calculated between values before and after pH cycling.ResultsMean (95%CI) Δ Z _B was 3851 (3762;3939) vol% × μm. Except for NaF-gel_2 and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel_2 induced a significant gain in mineral content ( p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔ Z _E1 and for ΔΔ Z _E2 ( p
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effect of naf amf kf gels and naf toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyerlueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p < 0.05, Bonferroni post hoc test). However, only NaF-gel and NaF-TP induced remineralization. CONCLUSION Under the in vitro conditions chosen, all fluoride agents could significantly hamper the adverse effects of a Demineralizing saliva substitute. CLINICAL SIGNIFICANCE In combination with a Demineralizing saliva substitute, slight mineral gain was only observed for neutral NaF-gel and 5000 ppm F toothpaste.
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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro.
Clinical Oral Investigations, 2018Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyer-lueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p
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progression of sealed initial bovine enamel lesions under Demineralizing conditions in vitro
Caries Research, 2006Co-Authors: Sebastian Paris, Hendrik Meyerlueckel, Jan Mueller, M Hummel, Andrej M KielbassaAbstract:The use of sealants for the infiltration of proximal enamel lesions could be a promising alternative to the common strategies of remineralization and operative treatment. The aim of the present study was to compare the progression of sealed initial enamel lesions after exposure to a Demineralizing solution in vitro. In each of 54 bovine enamel specimens three subsurface lesions were created. Two of the lesions were etched with phosphoric acid and sealed with either a fissure sealant or with various adhesives (1–5) for 15 s or 30 s, respectively, whereas one lesion remained as the untreated control. Subsequently, half of each specimen was covered with nail varnish (baseline) and the other half was reexposed to a Demineralizing solution for 14 days (experimental). The specimens were cut perpendicularly to the surface, infiltrated with a low-viscosity fluorescent resin and observed with a confocal laser scanning microscope (CLSM). For lesions sealed with the fissure sealant and adhesives 1–3, the progression of lesion depth (0–31 µm) was significantly decreased (p
Carolin Walther - One of the best experts on this subject based on the ideXlab platform.
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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyer-lueckel, Sebastian Paris, Richard Johannes WierichsAbstract:ObjectiveThe aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany.MethodsIn each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (Δ Z _B). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F^− [pH = 6.04] (NaF-gel_1), 12.500 ppm F^− [pH = 7.34] (NaF-gel_2), 12.500 ppm F^− [pH = 5.82] (AmF-gel), 1450 ppm F^− [pH = 7.35] (KF-gel), and 5000 ppm F^− [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔ Z _E1/ΔΔ Z _E2) were calculated between values before and after pH cycling.ResultsMean (95%CI) Δ Z _B was 3851 (3762;3939) vol% × μm. Except for NaF-gel_2 and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel_2 induced a significant gain in mineral content ( p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔ Z _E1 and for ΔΔ Z _E2 ( p
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effect of naf amf kf gels and naf toothpaste combined with a saliva substitute on dentin lesions in vitro
Clinical Oral Investigations, 2019Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyerlueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p < 0.05, Bonferroni post hoc test). However, only NaF-gel and NaF-TP induced remineralization. CONCLUSION Under the in vitro conditions chosen, all fluoride agents could significantly hamper the adverse effects of a Demineralizing saliva substitute. CLINICAL SIGNIFICANCE In combination with a Demineralizing saliva substitute, slight mineral gain was only observed for neutral NaF-gel and 5000 ppm F toothpaste.
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Effect of NaF, AmF, KF gels and NaF toothpaste combined with a saliva substitute on dentin lesions in vitro.
Clinical Oral Investigations, 2018Co-Authors: Carolin Walther, Manana Kreibohm, Peter Tschoppe, Hendrik Meyer-lueckel, Sebastian Paris, Richard Johannes WierichsAbstract:OBJECTIVE The aim of the present in vitro study was to evaluate the remineralizing effects of NaF, AmF, KF gels and NaF toothpaste in combination with a potentially Demineralizing saliva substitute (Glandosane; pH = 5.1) being widely used in Germany. METHODS In each of 120 dentin specimens, three artificial lesions were created. One lesion was covered for analysis of pre-demineralization (ΔZ). Treatments during pH cycling (3 × 1 h demineralization/day [pH = 5.0] and 3 × 3 h Glandosane/day; 12 h 100%humidity) were as follows: no treatment (NT), application (5 min,2×/day) of 12.500 ppm F [pH = 6.04] (NaF-gel), 12.500 ppm F [pH = 7.34] (NaF-gel), 12.500 ppm F [pH = 5.82] (AmF-gel), 1450 ppm F [pH = 7.35] (KF-gel), and 5000 ppm F [pH = 8.14]; (NaF-TP) for 7 days (E1). Subsequently, from each specimen, one lesion was covered, while the remaining lesion was cycled for another 7 days (E2). Differences in integrated mineral loss (ΔΔZ/ΔΔZ) were calculated between values before and after pH cycling. RESULTS Mean (95%CI) ΔZ was 3851 (3762;3939) vol% × μm. Except for NaF-gel and NaF-TP, specimens of all other groups further demineralized. Only NaF-gel induced a significant gain in mineral content (p ≤ 0.004; paired t test). Significant differences in the change of mineral loss were found between NT and all fluoride groups for both ΔΔZ and for ΔΔZ (p