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C.-c. Wang - One of the best experts on this subject based on the ideXlab platform.
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The clinical differences between Dengue and scrub typhus with acute respiratory failure in southern Taiwan
Infection, 2012Co-Authors: H.-c. Chang, M.-c. Su, W.-f. Fang, Y.-c. Chen, C.-c. Tseng, K.-t. Huang, C.-c. WangAbstract:Background For both Dengue and scrub typhus, acute respiratory failure (ARF) is a serious complication. The present study was carried out in order to investigate the clinical courses and outcomes of adult Dengue and scrub typhus patients with ARF, and to identify the clinical differences between adult Dengue and scrub typhus patients with ARF. Methods We conducted a retrospective study of the serologically confirmed adult Dengue or scrub typhus patients admitted between 1998 and 2008 at Kaohsiung Chang Gung Memorial Hospital. A total of 980 Dengue and 102 scrub typhus adult patients were included in our study. Results Eighteen of the 980 adult Dengue patients and 8 of the 102 adult scrub typhus patients had ARF. There were significant differences that existed for eschar ( P = 0.001; Dengue 0%; scrub 62.5%), cough ( P = 0.016; Dengue 55.6%; scrub typhus 100%), white blood cell (WBC) count [ P = 0.026; Dengue 7.40 ± 5.74; scrub typhus 11.84 ± 4.95 (×10^3/μL)], platelet count [ P = 0.008; Dengue 42.2 ± 33.9; scrub typhus 104.1 ± 93.3 (×10^9/L)], prothrombin time (PT) [ P = 0.007; Dengue 12.82 ± 1.36; scrub typhus 10.74 ± 0.98 (s)], activated partial thromboplastin time (APTT) [ P = 0.002; Dengue 50.81 ± 10.08; scrub typhus 37.44 ± 4.06 (s)], blood urea nitrogen (BUN) [ P
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The clinical differences between Dengue and scrub typhus with acute respiratory failure in southern Taiwan
Infection, 2012Co-Authors: H.-c. Chang, W.-f. Fang, Y.-c. Chen, C.-c. Tseng, K.-t. Huang, M.-c. Lin, S.-f. Liu, C.-h. Lai, C.-c. WangAbstract:Background For both Dengue and scrub typhus, acute respiratory failure (ARF) is a serious complication. The present study was carried out in order to investigate the clinical courses and outcomes of adult Dengue and scrub typhus patients with ARF, and to identify the clinical differences between adult Dengue and scrub typhus patients with ARF. Methods We conducted a retrospective study of the serologically confirmed adult Dengue or scrub typhus patients admitted between 1998 and 2008 at Kaohsiung Chang Gung Memorial Hospital. A total of 980 Dengue and 102 scrub typhus adult patients were included in our study. Results Eighteen of the 980 adult Dengue patients and 8 of the 102 adult scrub typhus patients had ARF. There were significant differences that existed for eschar ( P = 0.001; Dengue 0%; scrub 62.5%), cough ( P = 0.016; Dengue 55.6%; scrub typhus 100%), white blood cell (WBC) count [ P = 0.026; Dengue 7.40 ± 5.74; scrub typhus 11.84 ± 4.95 (×10^3/μL)], platelet count [ P = 0.008; Dengue 42.2 ± 33.9; scrub typhus 104.1 ± 93.3 (×10^9/L)], prothrombin time (PT) [ P = 0.007; Dengue 12.82 ± 1.36; scrub typhus 10.74 ± 0.98 (s)], activated partial thromboplastin time (APTT) [ P = 0.002; Dengue 50.81 ± 10.08; scrub typhus 37.44 ± 4.06 (s)], blood urea nitrogen (BUN) [ P
Annelies Wildersmith - One of the best experts on this subject based on the ideXlab platform.
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severe Dengue in travellers pathogenesis risk and clinical management
Journal of Travel Medicine, 2019Co-Authors: Scott B Halstead, Annelies WildersmithAbstract:RATIONALE FOR REVIEW: Dengue is a frequent cause of febrile illness among travellers and has overtaken malaria as the leading cause of febrile illness for those traveling to Southeast Asia. The purpose is to review the risk of Dengue and severe Dengue in travellers with a particular focus on the pathogenesis and clinical management of severe Dengue. RISK, PATHOGENESIS AND CLINICAL MANAGEMENT: The risk of travel-acquired Dengue depends on destination, season and duration of travel and activities during travel. Seroconversion rates reported in travellers, therefore, vary between 20%. The most common life-threatening clinical response to Dengue infection is the Dengue vascular permeability syndrome, epidemiologically linked to secondary infection, but can also occur in primary infection. Tertiary and quaternary infections are usually associated with mild or no disease. Antibody-dependent enhancement, viral factors, age, host factors and clinical experience of the managing physician modulate the risk of progressing to severe Dengue. The relative risk of severe Dengue in secondary versus primary infection ranges from 2 to 7. The absolute risk of severe Dengue in children in highly endemic areas is ~0.1% per year for primary infections and 0.4% for secondary infections. About 2-4% of secondary infections lead to severe Dengue. Severe Dengue and death are both relatively rare in general travellers but more frequently in those visiting friends and relatives. Clinical management of severe Dengue depends on judicious use of fluid rehydration. CONCLUSIONS: Although Dengue is a frequent cause of travel illness, severe Dengue and deaths are rare. Nevertheless, Dengue infections can interrupt travel and lead to evacuation and major out-of-pocket costs. Dengue is more frequent than many other travel-related vaccine preventable diseases, such as hepatitis A, hepatitis B, rabies, Japanese encephalitis and yellow fever, indicating a need for a Dengue vaccine for travellers.
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severe Dengue in travellers pathogenesis risk and clinical management
Journal of Travel Medicine, 2019Co-Authors: Scott B Halstead, Annelies WildersmithAbstract:RATIONALE FOR REVIEW: Dengue is a frequent cause of febrile illness among travellers and has overtaken malaria as the leading cause of febrile illness for those traveling to Southeast Asia. The purpose is to review the risk of Dengue and severe Dengue in travellers with a particular focus on the pathogenesis and clinical management of severe Dengue. RISK, PATHOGENESIS AND CLINICAL MANAGEMENT: The risk of travel-acquired Dengue depends on destination, season and duration of travel and activities during travel. Seroconversion rates reported in travellers, therefore, vary between 20%. The most common life-threatening clinical response to Dengue infection is the Dengue vascular permeability syndrome, epidemiologically linked to secondary infection, but can also occur in primary infection. Tertiary and quaternary infections are usually associated with mild or no disease. Antibody-dependent enhancement, viral factors, age, host factors and clinical experience of the managing physician modulate the risk of progressing to severe Dengue. The relative risk of severe Dengue in secondary versus primary infection ranges from 2 to 7. The absolute risk of severe Dengue in children in highly endemic areas is ~0.1% per year for primary infections and 0.4% for secondary infections. About 2-4% of secondary infections lead to severe Dengue. Severe Dengue and death are both relatively rare in general travellers but more frequently in those visiting friends and relatives. Clinical management of severe Dengue depends on judicious use of fluid rehydration. CONCLUSIONS: Although Dengue is a frequent cause of travel illness, severe Dengue and deaths are rare. Nevertheless, Dengue infections can interrupt travel and lead to evacuation and major out-of-pocket costs. Dengue is more frequent than many other travel-related vaccine preventable diseases, such as hepatitis A, hepatitis B, rabies, Japanese encephalitis and yellow fever, indicating a need for a Dengue vaccine for travellers.
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chymase level is a predictive biomarker of Dengue hemorrhagic fever in pediatric and adult patients
The Journal of Infectious Diseases, 2017Co-Authors: Hasitha Tissera, Duane J. Gubler, Abhay P S Rathore, Wei Yee Leong, Brian L Pike, Tyler E Warkentien, Farouk S Farouk, Ayesa Syenina, Eng Eong Ooi, Annelies WildersmithAbstract:Background. Most patients with Dengue experience mild disease, Dengue fever (DF), while few develop the life-threatening diseases Dengue hemorrhagic fever (DHF) or Dengue shock syndrome (DSS). No l ...
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reviewing Dengue still a neglected tropical disease
PLOS Neglected Tropical Diseases, 2015Co-Authors: Olaf Horstick, Yesim Tozan, Annelies WildersmithAbstract:Dengue is currently listed as a “neglected tropical disease” (NTD). But is Dengue still an NTD or not? Classifying Dengue as an NTD may carry advantages, but is it justified? This review considers the criteria for the definition of an NTD, the current diverse lists of NTDs by different stakeholders, and the commonalities and differences of Dengue with other NTDs. We also review the current research gaps and research activities and the adequacy of funding for Dengue research and development (R&D) (2003–2013). NTD definitions have been developed to a higher precision since the early 2000s, with the following main features: NTDs are characterised as a) poverty related, b) endemic to the tropics and subtropics, c) lacking public health attention, d) having poor research funding and shortcomings in R&D, e) usually associated with high morbidity but low mortality, and f) often having no specific treatment available. Dengue meets most of these criteria, but not all. Although Dengue predominantly affects resource-limited countries, it does not necessarily only target the poor and marginalised in those countries. Dengue increasingly attracts public health attention, and in some affected countries it is now a high profile disease. Research funding for Dengue has increased exponentially in the past two decades, in particular in the area of Dengue vaccine development. However, despite advances in Dengue research, Dengue epidemics are increasing in frequency and magnitude, and Dengue is expanding to new areas. Specific treatment and a highly effective vaccine remain elusive. Major research gaps exist in the area of integrated surveillance and vector control. Hence, although Dengue differs from many of the NTDs, it still meets important criteria commonly used for NTDs. The current need for increased R&D spending, shared by Dengue and other NTDs, is perhaps the key reason why Dengue should continue to be considered an NTD.
Frederic Bedin - One of the best experts on this subject based on the ideXlab platform.
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Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with Dengue fever or severe Dengue
BMC Infectious Diseases, 2015Co-Authors: Romain Fragnoud, Alexandre Pachot, Marie Flamand, Frederic Reynier, Vasna Duong, Philippe Buchy, Glaucia Paranhos-baccala, Frederic BedinAbstract:Dengue is the most widespread mosquito-borne viral disease of public health concern. In some patients, endothelial cell and platelet dysfunction lead to life-threatening hemorrhagic Dengue fever or Dengue shock syndrome. Prognostication of disease severity is urgently required to improve patient management. The pathogenesis of severe Dengue has not been fully elucidated, and the role of host proteins associated with viral particles has received little exploration. The proteomes of virion-enriched fractions purified from plasma pools of patients with Dengue fever or severe Dengue were compared. Virions were purified by ultracentrifugation combined with a water-insoluble polyelectrolyte-based technique. Following in-gel hydrolysis, peptides were analyzed by nano-liquid chromatography coupled to ion trap mass spectrometry and identified using data libraries. Both Dengue fever and severe Dengue viral-enriched fractions contained identifiable viral envelope proteins and host cellular proteins. Canonical pathway analysis revealed the identified host proteins are mainly involved in the coagulation cascade, complement pathway or acute phase response signaling pathway. Some host proteins were over- or under-represented in plasma from patients with severe Dengue compared to patients with Dengue fever. ELISAs were used to validate differential expression of a selection of identified host proteins in individual plasma samples of patients with Dengue fever compared to patients with severe Dengue. Among 22 host proteins tested, two could differentiate between Dengue fever and severe Dengue in two independent cohorts (olfactomedin-4: area under the curve (AUC), 0.958; and platelet factor-4: AUC, 0.836). A novel technique of virion-enrichment from plasma has allowed to identify two host proteins that have prognostic value for classifying patients with acute Dengue who are more likely to develop a severe Dengue. The impact of these host proteins on pathogenicity and disease outcome are discussed.
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Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with Dengue fever or severe Dengue
BMC Infectious Diseases, 2015Co-Authors: Romain Fragnoud, Alexandre Pachot, Marie Flamand, Frederic Reynier, Vasna Duong, Philippe Buchy, Glaucia Paranhos-baccala, Frederic BedinAbstract:Background: Dengue is the most widespread mosquito-borne viral disease of public health concern. In some patients, endothelial cell and platelet dysfunction lead to life-threatening hemorrhagic Dengue fever or Dengue shock syndrome. Prognostication of disease severity is urgently required to improve patient management. The pathogenesis of severe Dengue has not been fully elucidated, and the role of host proteins associated with viral particles has received little exploration. Methods: The proteomes of virion-enriched fractions purified from plasma pools of patients with Dengue fever or severe Dengue were compared. Virions were purified by ultracentrifugation combined with a water-insoluble polyelectrolyte-based technique. Following in-gel hydrolysis, peptides were analyzed by nano-liquid chromatography coupled to ion trap mass spectrometry and identified using data libraries. Results: Both Dengue fever and severe Dengue viral-enriched fractions contained identifiable viral envelope proteins and host cellular proteins. Canonical pathway analysis revealed the identified host proteins are mainly involved in the coagulation cascade, complement pathway or acute phase response signaling pathway. Some host proteins were over- or under-represented in plasma from patients with severe Dengue compared to patients with Dengue fever. ELISAs were used to validate differential expression of a selection of identified host proteins in individual plasma samples of patients with Dengue fever compared to patients with severe Dengue. Among 22 host proteins tested, two could differentiate between Dengue fever and severe Dengue in two independent cohorts (olfactomedin-4: area under the curve (AUC), 0.958; and platelet factor-4: AUC, 0.836). Conclusion: A novel technique of virion-enrichment from plasma has allowed to identify two host proteins that have prognostic value for classifying patients with acute Dengue who are more likely to develop a severe Dengue. The impact of these host proteins on pathogenicity and disease outcome are discussed.
Marie Flamand - One of the best experts on this subject based on the ideXlab platform.
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Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with Dengue fever or severe Dengue
BMC Infectious Diseases, 2015Co-Authors: Romain Fragnoud, Alexandre Pachot, Marie Flamand, Frederic Reynier, Vasna Duong, Philippe Buchy, Glaucia Paranhos-baccala, Frederic BedinAbstract:Dengue is the most widespread mosquito-borne viral disease of public health concern. In some patients, endothelial cell and platelet dysfunction lead to life-threatening hemorrhagic Dengue fever or Dengue shock syndrome. Prognostication of disease severity is urgently required to improve patient management. The pathogenesis of severe Dengue has not been fully elucidated, and the role of host proteins associated with viral particles has received little exploration. The proteomes of virion-enriched fractions purified from plasma pools of patients with Dengue fever or severe Dengue were compared. Virions were purified by ultracentrifugation combined with a water-insoluble polyelectrolyte-based technique. Following in-gel hydrolysis, peptides were analyzed by nano-liquid chromatography coupled to ion trap mass spectrometry and identified using data libraries. Both Dengue fever and severe Dengue viral-enriched fractions contained identifiable viral envelope proteins and host cellular proteins. Canonical pathway analysis revealed the identified host proteins are mainly involved in the coagulation cascade, complement pathway or acute phase response signaling pathway. Some host proteins were over- or under-represented in plasma from patients with severe Dengue compared to patients with Dengue fever. ELISAs were used to validate differential expression of a selection of identified host proteins in individual plasma samples of patients with Dengue fever compared to patients with severe Dengue. Among 22 host proteins tested, two could differentiate between Dengue fever and severe Dengue in two independent cohorts (olfactomedin-4: area under the curve (AUC), 0.958; and platelet factor-4: AUC, 0.836). A novel technique of virion-enrichment from plasma has allowed to identify two host proteins that have prognostic value for classifying patients with acute Dengue who are more likely to develop a severe Dengue. The impact of these host proteins on pathogenicity and disease outcome are discussed.
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Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with Dengue fever or severe Dengue
BMC Infectious Diseases, 2015Co-Authors: Romain Fragnoud, Alexandre Pachot, Marie Flamand, Frederic Reynier, Vasna Duong, Philippe Buchy, Glaucia Paranhos-baccala, Frederic BedinAbstract:Background: Dengue is the most widespread mosquito-borne viral disease of public health concern. In some patients, endothelial cell and platelet dysfunction lead to life-threatening hemorrhagic Dengue fever or Dengue shock syndrome. Prognostication of disease severity is urgently required to improve patient management. The pathogenesis of severe Dengue has not been fully elucidated, and the role of host proteins associated with viral particles has received little exploration. Methods: The proteomes of virion-enriched fractions purified from plasma pools of patients with Dengue fever or severe Dengue were compared. Virions were purified by ultracentrifugation combined with a water-insoluble polyelectrolyte-based technique. Following in-gel hydrolysis, peptides were analyzed by nano-liquid chromatography coupled to ion trap mass spectrometry and identified using data libraries. Results: Both Dengue fever and severe Dengue viral-enriched fractions contained identifiable viral envelope proteins and host cellular proteins. Canonical pathway analysis revealed the identified host proteins are mainly involved in the coagulation cascade, complement pathway or acute phase response signaling pathway. Some host proteins were over- or under-represented in plasma from patients with severe Dengue compared to patients with Dengue fever. ELISAs were used to validate differential expression of a selection of identified host proteins in individual plasma samples of patients with Dengue fever compared to patients with severe Dengue. Among 22 host proteins tested, two could differentiate between Dengue fever and severe Dengue in two independent cohorts (olfactomedin-4: area under the curve (AUC), 0.958; and platelet factor-4: AUC, 0.836). Conclusion: A novel technique of virion-enrichment from plasma has allowed to identify two host proteins that have prognostic value for classifying patients with acute Dengue who are more likely to develop a severe Dengue. The impact of these host proteins on pathogenicity and disease outcome are discussed.
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evaluation of an enzyme immunoassay for detection of Dengue virus ns1 antigen in human serum
Clinical and Vaccine Immunology, 2006Co-Authors: Philippe Dussart, Bhety Labeau, Gisele Lagathu, Philippe Louis, Marcio Roberto Teixeira Nunes, Sueli Rodrigues, Cecile Storckherrmann, Raymond Cesaire, Jacques Morvan, Marie FlamandAbstract:We evaluated a one-step sandwich-format microplate enzyme immunoassay for detecting Dengue virus NS1 antigen (Ag) in human serum by use of Platelia Dengue NS1 Ag kits (Bio-Rad Laboratories, Marnes La Coquette, France). We collected 299 serum samples from patients with Dengue disease and 50 serum samples from patients not infected with Dengue virus. For the 239 serum samples from patients with acute infections testing positive by reverse transcription-PCR and/or virus isolation for one of the four Dengue virus serotypes, the sensitivity of the Platelia Dengue NS1 Ag kit was 88.7% (95% confidence interval, 84.0% to 92.4%). None of the serum samples from patients not infected with Dengue virus tested positive with the Platelia Dengue NS1 Ag kit. A diagnostic strategy combining the Platelia Dengue NS1 Ag test for acute-phase sera and immunoglobulin M capture enzyme-linked immunosorbent assay for early-convalescent-phase sera increased sensitivity only from 88.7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute Dengue virus infection in clinical diagnostic laboratories.
H.-c. Chang - One of the best experts on this subject based on the ideXlab platform.
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The clinical differences between Dengue and scrub typhus with acute respiratory failure in southern Taiwan
Infection, 2012Co-Authors: H.-c. Chang, M.-c. Su, W.-f. Fang, Y.-c. Chen, C.-c. Tseng, K.-t. Huang, C.-c. WangAbstract:Background For both Dengue and scrub typhus, acute respiratory failure (ARF) is a serious complication. The present study was carried out in order to investigate the clinical courses and outcomes of adult Dengue and scrub typhus patients with ARF, and to identify the clinical differences between adult Dengue and scrub typhus patients with ARF. Methods We conducted a retrospective study of the serologically confirmed adult Dengue or scrub typhus patients admitted between 1998 and 2008 at Kaohsiung Chang Gung Memorial Hospital. A total of 980 Dengue and 102 scrub typhus adult patients were included in our study. Results Eighteen of the 980 adult Dengue patients and 8 of the 102 adult scrub typhus patients had ARF. There were significant differences that existed for eschar ( P = 0.001; Dengue 0%; scrub 62.5%), cough ( P = 0.016; Dengue 55.6%; scrub typhus 100%), white blood cell (WBC) count [ P = 0.026; Dengue 7.40 ± 5.74; scrub typhus 11.84 ± 4.95 (×10^3/μL)], platelet count [ P = 0.008; Dengue 42.2 ± 33.9; scrub typhus 104.1 ± 93.3 (×10^9/L)], prothrombin time (PT) [ P = 0.007; Dengue 12.82 ± 1.36; scrub typhus 10.74 ± 0.98 (s)], activated partial thromboplastin time (APTT) [ P = 0.002; Dengue 50.81 ± 10.08; scrub typhus 37.44 ± 4.06 (s)], blood urea nitrogen (BUN) [ P
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The clinical differences between Dengue and scrub typhus with acute respiratory failure in southern Taiwan
Infection, 2012Co-Authors: H.-c. Chang, W.-f. Fang, Y.-c. Chen, C.-c. Tseng, K.-t. Huang, M.-c. Lin, S.-f. Liu, C.-h. Lai, C.-c. WangAbstract:Background For both Dengue and scrub typhus, acute respiratory failure (ARF) is a serious complication. The present study was carried out in order to investigate the clinical courses and outcomes of adult Dengue and scrub typhus patients with ARF, and to identify the clinical differences between adult Dengue and scrub typhus patients with ARF. Methods We conducted a retrospective study of the serologically confirmed adult Dengue or scrub typhus patients admitted between 1998 and 2008 at Kaohsiung Chang Gung Memorial Hospital. A total of 980 Dengue and 102 scrub typhus adult patients were included in our study. Results Eighteen of the 980 adult Dengue patients and 8 of the 102 adult scrub typhus patients had ARF. There were significant differences that existed for eschar ( P = 0.001; Dengue 0%; scrub 62.5%), cough ( P = 0.016; Dengue 55.6%; scrub typhus 100%), white blood cell (WBC) count [ P = 0.026; Dengue 7.40 ± 5.74; scrub typhus 11.84 ± 4.95 (×10^3/μL)], platelet count [ P = 0.008; Dengue 42.2 ± 33.9; scrub typhus 104.1 ± 93.3 (×10^9/L)], prothrombin time (PT) [ P = 0.007; Dengue 12.82 ± 1.36; scrub typhus 10.74 ± 0.98 (s)], activated partial thromboplastin time (APTT) [ P = 0.002; Dengue 50.81 ± 10.08; scrub typhus 37.44 ± 4.06 (s)], blood urea nitrogen (BUN) [ P
