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Luiz Guilherme Brentegani - One of the best experts on this subject based on the ideXlab platform.
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Bone integration behavior of hydroxyapatite/β-tricalcium phosphate graft implanted in Dental alveoli: a histomorphometric and scanning electron microscopy study.
Implant dentistry, 2014Co-Authors: Rander Moreira Macedo, Suzie Aparecida De Lacerda, José Antônio Thomazini, Luiz Guilherme BrenteganiAbstract:PURPOSE This study investigated the bone integration ability of a biphasic calcium phosphate bioceramic implanted in Dental Alveolus of rats. MATERIALS AND METHODS A total of 21 male rats were submitted to upper right incisor extraction and implanted with a synthetic bioceramic (Straumann Bone Ceramic). The animals were killed on 7th, 21st, and 42nd day after surgery for light and scanning electron microscopy (SEM) analysis of bone, bioceramic, and soft tissue volume as well as the quality of graft in its interface. RESULTS Light histology results showed no persistent inflammatory and foreign body reactions, a newly formed bone adhered on the ceramic surface without interposition of soft tissue, which was confirmed by SEM analysis. Histometrically, reduction/resorption, between 7 and 42 days, in the percentage of bioceramic implanted (α = 1%) left gaps for a gradual increase in vital bone formation (α = 1%) around the particles. CONCLUSIONS The bioceramic in question is biocompatible, has good bone integration, being gradually resorbed and replaced by it, featuring a viable bone substitute for grafting procedures.
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Biocompatibility of natural latex implanted into Dental Alveolus of rats.
Journal of oral science, 2006Co-Authors: Cláudia Andréa C. Albuquerque Balabanian, Joaquim Coutinho-netto, Teresa Lúcia Lamano-carvalho, Suzie Aparecida De Lacerda, Luiz Guilherme BrenteganiAbstract:The present study investigated the biocompatibility of a biopolymer based on vegetable latex extracted from the Hevea brasiliensis rubber tree, implanted into the bony alveolar cavity after Dental extraction in rats. A granule of latex (area = 0.25 +/- 0.04 mm(2)) was implanted inside the Alveolus immediately after extraction of the upper right incisor, and the animals were sacrificed 7, 21 and 42 days after the procedure. The hemi-maxillas were decalcified and processed for embedding in paraffin to obtain semi-serial longitudinal sections 5 mum thick, and then stained with hematoxylin-eosin. The latex granule was observed in the cervical third of the Alveolus without any foreign body reaction, or persistence of the initial acute inflammatory reaction. Bone repair in the areas adjacent to the material was quantified, and a decrease was noted in the thickness of the fibrous capsule surrounding the implants from 92.8 +/- 9.3 microm on day 7 to 9.4 +/- 1.8 microm on day 42 (ANOVA, P = 0.01). The quantitative data confirmed acceleration of bone formation (statistically significant at 5%) in parallel with a decrease of connective tissue in the areas around the implants. These results show that the tested material is biologically compatible, and progressively integrated into the alveolar bone, simultaneously accelerating bone formation and playing an important role in the healing process.
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Implante de um floculado de resina de mamona em alvéolo Dental de rato
Pesquisa odontologica brasileira = Brazilian oral research, 2001Co-Authors: Romeu Felipe Elias Calixto, Luiz Guilherme Brentegani, Juliana Mazzonetto Teófilo, Teresa Lúcia Lamano CarvalhoAbstract:The purposes of the present study were: 1) to investigate the biocompatibility of a natural resin (made of fatty acids extracted from Ricinus communis) implanted in the Dental Alveolus of rats and 2) to verify any possible interference of that material in the osseous healing following tooth extraction. The resin (AUG-EX, Poliquil Araraquara Polimeros Quimicos LTDA, Araraquara - SP) was placed inside de alveoli immediately after extraction of the upper right incisors. The animals were sacrificed 1, 2, 3 and 6 weeks after extraction or extraction + implantation. The hemi-maxillae were decalcified and processed for paraffin embedding. Longitudinal 6-micrometer-thick semi-serial sections stained with hematoxylin and eosin were obtained. Histologic examination showed particles of irregular shape and variable size (700-1200 mm) localized in the medium/cervical alveolar thirds, with a scanty but persistent foreign body reaction. From the second week on, as the relative volume of bone trabeculae increased, it was seen in close contact with the surface of the implanted material in some regions. Histometric analysis (differential point counting method), used to quantify the healing process in the apical third, showed a small but significant decrease (13%-20%) in new bone formation in the implanted rats. In conclusion, the results show that, in spite of its biocompatible nature, the studied resin hinders the post-extration healing process.
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implante de um floculado de resina de mamona em alvoolo Dental de rato implantation of flakes of castor oil resin in rat Dental Alveolus
2001Co-Authors: Romeu Felipe, Luiz Guilherme Brentegani, Elias Calixto, Juliana Mazzonetto, Teresa Lœcia, Lamano CarvalhoAbstract:, v. 15, n. 3, p. 257-262, jul./set. 2001.Osobjetivosdopresentetrabalhoforam:1)testarabiocompatibilidadedeumaresinanatural,derivadadooleodema-mona, implantada na cavidade de extracao Dental de ratos, e 2) estudar a possivel interferŒncia do material na crono-logia do reparo alveolar. O material (AUG-EX, Poliquil Araraquara Polimeros Quimicos Ltda., Araraquara - SP) foi im-plantado no alvOolo imediatamente apos a extracao do incisivo superior direito e os ratos foram sacrificados de1a6semanas apos a extracao ou extracao + implante. As hemimaxilas foram descalcificadas e processadas para inclusaoem parafina e obtencao de cortes semi-seriados, corados com hematoxilina-eosina. Os flocos da resina, de forma irre-gularetamanhovariAEvel,localizaram-seentreostercosalveolaresmOdioecervical,inicialmentecircundadosporteci-dodegranulacaoeaseguirporquantidadeprogressivamentemaiordetecidoosseo,nogeralcomapresencadeumte-cido conjuntivo interposto, mas em algumas AEreas estabelecendo aparente osseointegracao direta. Nao houvepersistŒnciadareacaoinflamatoria,masobservou-sepequenaquantidadedecOlulasgigantesaderidasasuperficiedomaterial, em todos os periodos. A anAElise histomOtrica (contagem diferencial de pontos) do terco apical mostrou umatrasode13%a20%noreparoalveolardosratosimplantados,commenorneoformacaoosseaassociadaamaioresvo-lumes percentuais de tecido conjuntivo e de remanescentes do coAEgulo sanguineo.UNITERMOS: Biocompatibilidade;
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Histologic and histometric evaluation of rat alveolar wound healing around polyurethane resin implants
International journal of oral and maxillofacial surgery, 1997Co-Authors: Teresa Lúcia Lamano Carvalho, C A C A Araujo, Juliana Mazzonetto Teófilo, Luiz Guilherme BrenteganiAbstract:Abstract The biocompatibility of polyurethane resin implants derived from castor bean ( Ricinus communis ) was analyzed in the rat Dental Alveolus. Histometric evaluation of trial areas adjacent to the implants showed, by week 1, the polymer granules encircled by a conspicuous capsule and surrounded by immature connective tissue. By weeks 2 and 3, the implants were surrounded by less prominent fibrous capsules, and most of the tested area was occupied by mature trabecular bone. By week 6, the fibrous capsule was thinner, and the tested area was almost totally covered with bone, which in several places was in close contact with the implants. The results suggest that the material is compatible, as it was progressively integrated into alveolar bone in the wound-healing process.
Pablo Galindo-moreno - One of the best experts on this subject based on the ideXlab platform.
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Biofunctionalization with a TGFβ-1 Inhibitor Peptide in the Osseointegration of Synthetic Bone Grafts: An In Vivo Study in Beagle Dogs
Materials, 2019Co-Authors: Andrea Cirera, Maria Cristina Manzanares, Monica Ortiz-hernandez, Pablo Sevilla, Pablo Galindo-morenoAbstract:Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial—one untreated and another submitted to biofunctionalization with a TGF-β1 inhibitor peptide, P144, on Dental Alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial’s granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial’s granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant’s osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of Dental biomaterials.
Teresa Lúcia Lamano Carvalho - One of the best experts on this subject based on the ideXlab platform.
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Implante de um floculado de resina de mamona em alvéolo Dental de rato
Pesquisa odontologica brasileira = Brazilian oral research, 2001Co-Authors: Romeu Felipe Elias Calixto, Luiz Guilherme Brentegani, Juliana Mazzonetto Teófilo, Teresa Lúcia Lamano CarvalhoAbstract:The purposes of the present study were: 1) to investigate the biocompatibility of a natural resin (made of fatty acids extracted from Ricinus communis) implanted in the Dental Alveolus of rats and 2) to verify any possible interference of that material in the osseous healing following tooth extraction. The resin (AUG-EX, Poliquil Araraquara Polimeros Quimicos LTDA, Araraquara - SP) was placed inside de alveoli immediately after extraction of the upper right incisors. The animals were sacrificed 1, 2, 3 and 6 weeks after extraction or extraction + implantation. The hemi-maxillae were decalcified and processed for paraffin embedding. Longitudinal 6-micrometer-thick semi-serial sections stained with hematoxylin and eosin were obtained. Histologic examination showed particles of irregular shape and variable size (700-1200 mm) localized in the medium/cervical alveolar thirds, with a scanty but persistent foreign body reaction. From the second week on, as the relative volume of bone trabeculae increased, it was seen in close contact with the surface of the implanted material in some regions. Histometric analysis (differential point counting method), used to quantify the healing process in the apical third, showed a small but significant decrease (13%-20%) in new bone formation in the implanted rats. In conclusion, the results show that, in spite of its biocompatible nature, the studied resin hinders the post-extration healing process.
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Histologic and histometric evaluation of rat alveolar wound healing around polyurethane resin implants
International journal of oral and maxillofacial surgery, 1997Co-Authors: Teresa Lúcia Lamano Carvalho, C A C A Araujo, Juliana Mazzonetto Teófilo, Luiz Guilherme BrenteganiAbstract:Abstract The biocompatibility of polyurethane resin implants derived from castor bean ( Ricinus communis ) was analyzed in the rat Dental Alveolus. Histometric evaluation of trial areas adjacent to the implants showed, by week 1, the polymer granules encircled by a conspicuous capsule and surrounded by immature connective tissue. By weeks 2 and 3, the implants were surrounded by less prominent fibrous capsules, and most of the tested area was occupied by mature trabecular bone. By week 6, the fibrous capsule was thinner, and the tested area was almost totally covered with bone, which in several places was in close contact with the implants. The results suggest that the material is compatible, as it was progressively integrated into alveolar bone in the wound-healing process.
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Histological evaluation of the biocompatibility of a glass-ionomer cement in rat Alveolus
Biomaterials, 1997Co-Authors: Luiz Guilherme Brentegani, K F Bombonato, Teresa Lúcia Lamano CarvalhoAbstract:A type III glass-ionomer cement (Vidrion F), currently used as fast-setting lining material and fissure sealant, was implanted into rat Dental Alveolus immediately after tooth extraction and its biocompatibility was analysed in terms of incorporation into alveolar bone in the wound healing process. Histological and histometric evaluation of trial areas adjacent to the implants showed that by week 1 the glass-ionomer granules were encircled by a conspicuous capsule surrounded by immature connective tissue. By week 3 the implants were surrounded by a less prominent fibrous capsule and most of the tested area was occupied by mature trabecular bone. By week 6 the fibrous capsule was thinner and the tested area was almost totally covered by bone, which was in close contact with the implanted material in several places. Quantitative data confirmed progressive new bone formation in parallel with a decrease in the percentage fraction of connective tissue in the trial areas around the implants. The results revealed that the tested material is biologically compatible, being progressively incorporated into alveolar bone in the wound healing process. The quantitative evaluation of alveolar wound healing around a glass-ionomer implant may provide an experimental model for future comparative studies carried out with other biomaterials.
Gennadi I. Mikhasev - One of the best experts on this subject based on the ideXlab platform.
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Viscoelastic Behavior of Periodontal Ligament: Stresses Relaxation at Translational Displacement of a Tooth Root
Trends in Mathematics, 2018Co-Authors: Sergei Bosiakov, Gennadi I. Mikhasev, Sergei V RogosinAbstract:Understanding of viscoelastic response of a periodontal membrane under the action of short-term and long-term loadings is important for many orthodontic problems. A new analytic model describing behavior of the viscoelastic periodontal ligament after the tooth root translational displacement based on Maxwell approach is suggested. In the model, a tooth root and alveolar bone are assumed to be a rigid bodies. The system of differential equations for the plane-strain state of the viscoelastic periodontal ligament is used as the governing one. The boundary conditions corresponding to the initial small displacement of the root and fixed outer surface of the periodontal ligament in the Dental Alveolus are utilized. A solution is found numerically for fractional viscoelasticity model assuming that the stress relaxation in the periodontal ligament after the continuing displacement of the tooth root occurs approximately within five hours. The character of stress distribution in the ligament over time caused by the tooth root translational displacement is evaluated. Effect of Poisson’s ratio on the stresses in the viscoelastic periodontal ligament is considered. The obtained results can be used for simulation of the bone remodelling process during orthodontic treatment and for assessment of optimal conditions of the orthodontic load application.
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Mathematical Model for Analysis of Translational Displacements of Tooth Root
Mathematical Modelling and Analysis, 2015Co-Authors: Sergei Bosiakov, Gennadi I. MikhasevAbstract:AbstractAnalytical modeling of stress-strain state of a periodontal ligament in the case of the translational displacement of a tooth root was carried out. The tooth root was assumed as a rigid body. The boundary conditions corresponding to the translational displacement of the root and fixed external surface of the periodontal ligament in the Dental Alveolus were considered. The system of differential equations describing the periodontal ligament's plane-strain state induced by the translational motion of the tooth were used as the governing equations. An analytical solution was found for the governing equations in the explicit form. Comparative analysis of the concentrated force generated by the prescribed translational motion of the tooth root was performed using the obtained analytical solution and the model of an incompressible periodontal ligament in the form of a circular paraboloid and hyperboloid. The mathematical model developed in this paper can be used to analyze stresses and strains in the pe...
Andrea Cirera - One of the best experts on this subject based on the ideXlab platform.
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Biofunctionalization with a TGFβ-1 Inhibitor Peptide in the Osseointegration of Synthetic Bone Grafts: An In Vivo Study in Beagle Dogs
Materials, 2019Co-Authors: Andrea Cirera, Maria Cristina Manzanares, Monica Ortiz-hernandez, Pablo Sevilla, Pablo Galindo-morenoAbstract:Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial—one untreated and another submitted to biofunctionalization with a TGF-β1 inhibitor peptide, P144, on Dental Alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial’s granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial’s granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant’s osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of Dental biomaterials.