The Experts below are selected from a list of 421218 Experts worldwide ranked by ideXlab platform
Ken-tye Yong - One of the best experts on this subject based on the ideXlab platform.
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Rapid SERS monitoring of lipid-peroxidation-Derived Protein modifications in cells using photonic crystal fiber sensor
Journal of Biophotonics, 2016Co-Authors: Tianxun Gong, Nan Zhang, Kien Voon Kong, Douglas Goh, Ying Cui, Jean-louis Auguste, Perry Ping Shum, Lei Wei, Georges Humbert, Ken-tye YongAbstract:We proposed a side channel photonic crystal fiber (SC-PCF) based Surface enhanced Raman spectroscopy (SERS) platform which is able to accurately monitor lipid peroxidation Derived Protein modifications in cells. This platform incorporates linoleamide alkyne (LAA), which is oxidized and subsequently modifies Proteins in cells with alkyne functional group upon lipid peroxidation. By loading the side channel of SC-PCF with a mixture of gold nanoparticles and LAA treated cells, and subsequently measuring the interference-free alkyne Raman peak from these Proteins in cells, strong SERS signal was obtained. The platform provides a method for the rapid monitoring of lipid peroxidation Derived Protein modification in cells.
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Rapid SERS monitoring of lipid‐peroxidation‐Derived Protein modifications in cells using photonic crystal fiber sensor
Journal of biophotonics, 2015Co-Authors: Tianxun Gong, Nan Zhang, Douglas Goh, Jean-louis Auguste, Lei Wei, Georges Humbert, Kien Voon Kong, Cui Ying, Perry Ping Shum, Ken-tye YongAbstract:We proposed a side channel photonic crystal fiber (SC-PCF) based Surface enhanced Raman spectroscopy (SERS) platform which is able to accurately monitor lipid peroxidation Derived Protein modifications in cells. This platform incorporates linoleamide alkyne (LAA), which is oxidized and subsequently modifies Proteins in cells with alkyne functional group upon lipid peroxidation. By loading the side channel of SC-PCF with a mixture of gold nanoparticles and LAA treated cells, and subsequently measuring the interference-free alkyne Raman peak from these Proteins in cells, strong SERS signal was obtained. The platform provides a method for the rapid monitoring of lipid peroxidation Derived Protein modification in cells.
Jean Benoît Peltier - One of the best experts on this subject based on the ideXlab platform.
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Proteomic characterisation of endoplasmic reticulum-Derived Protein bodies in tobacco leaves
BMC plant biology, 2012Co-Authors: Minu Joseph, M. Dolors Ludevid, Margarita Torrent, Valerie Rofidal, Marc Tauzin, Michel Rossignol, Jean Benoît PeltierAbstract:Background The N-terminal proline-rich domain (Zera) of the maize storage Protein γ-zein, is able to induce the formation of endoplasmic reticulum (ER)-Derived Protein bodies (PBs) when fused to Proteins of interest. This encapsulation enables a recombinant fused Protein to escape from degradation and facilitates its recovery from plant biomass by gradient purification. The aim of the present work was to evaluate if induced PBs encapsulate additional Proteins jointly with the recombinant Protein. The exhaustive analysis of Protein composition of PBs is expected to facilitate a better understanding of PB formation and the optimization of recombinant Protein purification approaches from these organelles.
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Proteomic characterisation of endoplasmic reticulum-Derived Protein bodies in tobacco leaves
BMC Plant Biology, 2012Co-Authors: Minu Joseph, M. Dolors Ludevid, Margarita Torrent, Valerie Rofidal, Marc Tauzin, Michel Rossignol, Jean Benoît PeltierAbstract:Background: The N-terminal proline-rich domain (Zera) of the maize storage Protein gamma-zein, is able to induce the formation of endoplasmic reticulum (ER)-Derived Protein bodies (PBs) when fused to Proteins of interest. This encapsulation enables a recombinant fused Protein to escape from degradation and facilitates its recovery from plant biomass by gradient purification. The aim of the present work was to evaluate if induced PBs encapsulate additional Proteins jointly with the recombinant Protein. The exhaustive analysis of Protein composition of PBs is expected to facilitate a better understanding of PB formation and the optimization of recombinant Protein purification approaches from these organelles. Results: We analysed the proteome of PBs induced in Nicotiana benthamiana leaves by transient transformation with Zera fused to a fluorescent marker Protein (DsRed). Intact PBs with their surrounding ER-membrane were isolated on iodixanol based density gradients and their integrity verified by confocal and electron microscopy. SDS-PAGE analysis of isolated PBs showed that Zera-DsRed accounted for around 85% of PB Proteins in term of abundance. Differential extraction of PBs was performed for in-depth analysis of their proteome and structure. Besides Zera-DsRed, 195 additional Proteins were identified including a broad range of Proteins resident or trafficking through the ER and recruited within the Zera-DsRed polymer. Conclusions: This study indicates that Zera-Protein fusion is still the major Protein component of the new formed organelle in tobacco leaves. The analysis also reveals the presence of an unexpected diversity of Proteins in PBs Derived from both the insoluble Zera-DsRed polymer formation, including ER-resident and secretory Proteins, and a secretory stress response induced most likely by the recombinant Protein overloading. Knowledge of PBs Protein composition is likely to be useful to optimize downstream purification of recombinant Proteins in molecular farming applications.
Roberto Bernabei - One of the best experts on this subject based on the ideXlab platform.
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animal Derived Protein consumption is associated with muscle mass and strength in community dwellers results from the milan expo survey
Journal of Nutrition Health & Aging, 2017Co-Authors: Francesco Landi, Riccardo Calvani, Matteo Tosato, Anna Maria Martone, Anna Picca, Elena Ortolani, Giulia Savera, Sara Salini, M Ramaschi, Roberto BernabeiAbstract:OBJECTIVES: Behavioral factors, including Protein intake, influence the quantity and quality of skeletal muscle. The aim of this study was to explore the relationship between animal-Derived Protein intake and muscle mass and function in a large sample of unselected community-dwellers. MATERIAL AND METHODS: The VIP (Very Important Protein) study, conducted during Expo 2015 in Milan, was a population survey aimed at assessing major health metrics in a population outside of the research setting, with a special focus on the relation between animal-Derived Protein intake and muscle mass and function. A brief questionnaire exploring lifestyle habits, dietary preferences and the consumption of selected foods was administered. Muscle mass was estimated by calf circumference (CC) and mid-arm muscle circumference (MAMC) of the dominant side. Muscle strength of upper and lower extremities was assessed through handgrip strength testing and repeated chair stand test, respectively. RESULTS: The mean age of the 1,853 participants was 50.3 years (standard deviation: 15.7; range: 18-98 years), of whom 959 (51.7%) were women. Participants in the highest tertile of Protein consumption showed better performance at both the handgrip strength (p <0.001) and chair stand tests than those in the lowest tertile (p <0.01). The same results were found for CC (p <0.001) and MAMC (p <0.001). Participants with high Protein intake and engaged in regular physical activity showed the higher scores in all the assessed domains. CONCLUSIONS: The results of the VIP survey suggest an association between animal-Derived Protein intake and muscle mass and strength across ages. Our findings also indicate a synergistic effect of animal-Derived Protein intake and physical activity on muscle-related parameters.
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Animal-Derived Protein consumption is associated with muscle mass and strength in community-dwellers: Results from the Milan Expo survey
The journal of nutrition health & aging, 2017Co-Authors: Francesco Landi, Riccardo Calvani, Matteo Tosato, Anna Maria Martone, Anna Picca, Elena Ortolani, Giulia Savera, Sara Salini, M Ramaschi, Roberto BernabeiAbstract:OBJECTIVES Behavioral factors, including Protein intake, influence the quantity and quality of skeletal muscle. The aim of this study was to explore the relationship between animal-Derived Protein intake and muscle mass and function in a large sample of unselected community-dwellers. MATERIAL AND METHODS The VIP (Very Important Protein) study, conducted during Expo 2015 in Milan, was a population survey aimed at assessing major health metrics in a population outside of the research setting, with a special focus on the relation between animal-Derived Protein intake and muscle mass and function. A brief questionnaire exploring lifestyle habits, dietary preferences and the consumption of selected foods was administered. Muscle mass was estimated by calf circumference (CC) and mid-arm muscle circumference (MAMC) of the dominant side. Muscle strength of upper and lower extremities was assessed through handgrip strength testing and repeated chair stand test, respectively. RESULTS The mean age of the 1,853 participants was 50.3 years (standard deviation: 15.7; range: 18-98 years), of whom 959 (51.7%) were women. Participants in the highest tertile of Protein consumption showed better performance at both the handgrip strength (p
Youssef Rouphael - One of the best experts on this subject based on the ideXlab platform.
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Understanding the Biostimulant Action of Vegetal-Derived Protein Hydrolysates by High-Throughput Plant Phenotyping and Metabolomics: A Case Study on Tomato
Frontiers Media S.A., 2019Co-Authors: Kenny Paul, Youssef Rouphael, Renaud Canaguier, Mariateresa Cardarelli, Mirella Sorrentino, Luigi Lucini, Paolo Bonini, Hélène Reynaud, Martin Trtílek, Klára PanzarováAbstract:Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective in finding new biostimulant substances. A screening protocol based on the use of high-throughput phenotyping platform for screening new vegetal-Derived Protein hydrolysates (PHs) for biostimulant activity followed by a metabolomic analysis to elucidate the mechanism of the most active PHs has been applied on tomato crop. Eight PHs (A–G, I) Derived from enzymatic hydrolysis of seed Proteins of Leguminosae and Brassicaceae species were foliarly sprayed twice during the trial. A non-ionic surfactant Triton X-100 at 0.1% was also added to the solutions before spraying. A control treatment foliarly sprayed with distilled water containing 0.1% Triton X-100 was also included. Untreated and PH-treated tomato plants were monitored regularly using high-throughput non-invasive imaging technologies. The phenotyping approach we used is based on automated integrative analysis of photosynthetic performance, growth analysis, and color index analysis. The digital biomass of the plants sprayed with PH was generally increased. In particular, the relative growth rate and the growth performance were significantly improved by PHs A and I, respectively, compared to the untreated control plants. Kinetic chlorophyll fluorescence imaging did not allow to differentiate the photosynthetic performance of treated and untreated plants. Finally, MS-based untargeted metabolomics analysis was performed in order to characterize the functional mechanisms of selected PHs. The treatment modulated the multi-layer regulation process that involved the ethylene precursor and polyamines and affected the ROS-mediated signaling pathways. Although further investigation is needed to strengthen our findings, metabolomic data suggest that treated plants experienced a metabolic reprogramming following the application of the tested biostimulants. Nonetheless, our experimental data highlight the potential for combined use of high-throughput phenotyping and metabolomics to facilitate the screening of new substances with biostimulant properties and to provide a morpho-physiological and metabolomic gateway to the mechanisms underlying PHs action on plants
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Table_4_Understanding the Biostimulant Action of Vegetal-Derived Protein Hydrolysates by High-Throughput Plant Phenotyping and Metabolomics: A Case Study on Tomato.DOCX
2019Co-Authors: Kenny Paul, Youssef Rouphael, Renaud Canaguier, Mariateresa Cardarelli, Mirella Sorrentino, Luigi Lucini, Paolo Bonini, Hélène Reynaud, Martin Trtílek, Klára PanzarováAbstract:Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective in finding new biostimulant substances. A screening protocol based on the use of high-throughput phenotyping platform for screening new vegetal-Derived Protein hydrolysates (PHs) for biostimulant activity followed by a metabolomic analysis to elucidate the mechanism of the most active PHs has been applied on tomato crop. Eight PHs (A–G, I) Derived from enzymatic hydrolysis of seed Proteins of Leguminosae and Brassicaceae species were foliarly sprayed twice during the trial. A non-ionic surfactant Triton X-100 at 0.1% was also added to the solutions before spraying. A control treatment foliarly sprayed with distilled water containing 0.1% Triton X-100 was also included. Untreated and PH-treated tomato plants were monitored regularly using high-throughput non-invasive imaging technologies. The phenotyping approach we used is based on automated integrative analysis of photosynthetic performance, growth analysis, and color index analysis. The digital biomass of the plants sprayed with PH was generally increased. In particular, the relative growth rate and the growth performance were significantly improved by PHs A and I, respectively, compared to the untreated control plants. Kinetic chlorophyll fluorescence imaging did not allow to differentiate the photosynthetic performance of treated and untreated plants. Finally, MS-based untargeted metabolomics analysis was performed in order to characterize the functional mechanisms of selected PHs. The treatment modulated the multi-layer regulation process that involved the ethylene precursor and polyamines and affected the ROS-mediated signaling pathways. Although further investigation is needed to strengthen our findings, metabolomic data suggest that treated plants experienced a metabolic reprogramming following the application of the tested biostimulants. Nonetheless, our experimental data highlight the potential for combined use of high-throughput phenotyping and metabolomics to facilitate the screening of new substances with biostimulant properties and to provide a morpho-physiological and metabolomic gateway to the mechanisms underlying PHs action on plants.
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Image_2_Understanding the Biostimulant Action of Vegetal-Derived Protein Hydrolysates by High-Throughput Plant Phenotyping and Metabolomics: A Case Study on Tomato.JPEG
2019Co-Authors: Kenny Paul, Youssef Rouphael, Renaud Canaguier, Mariateresa Cardarelli, Mirella Sorrentino, Luigi Lucini, Paolo Bonini, Hélène Reynaud, Martin Trtílek, Klára PanzarováAbstract:Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective in finding new biostimulant substances. A screening protocol based on the use of high-throughput phenotyping platform for screening new vegetal-Derived Protein hydrolysates (PHs) for biostimulant activity followed by a metabolomic analysis to elucidate the mechanism of the most active PHs has been applied on tomato crop. Eight PHs (A–G, I) Derived from enzymatic hydrolysis of seed Proteins of Leguminosae and Brassicaceae species were foliarly sprayed twice during the trial. A non-ionic surfactant Triton X-100 at 0.1% was also added to the solutions before spraying. A control treatment foliarly sprayed with distilled water containing 0.1% Triton X-100 was also included. Untreated and PH-treated tomato plants were monitored regularly using high-throughput non-invasive imaging technologies. The phenotyping approach we used is based on automated integrative analysis of photosynthetic performance, growth analysis, and color index analysis. The digital biomass of the plants sprayed with PH was generally increased. In particular, the relative growth rate and the growth performance were significantly improved by PHs A and I, respectively, compared to the untreated control plants. Kinetic chlorophyll fluorescence imaging did not allow to differentiate the photosynthetic performance of treated and untreated plants. Finally, MS-based untargeted metabolomics analysis was performed in order to characterize the functional mechanisms of selected PHs. The treatment modulated the multi-layer regulation process that involved the ethylene precursor and polyamines and affected the ROS-mediated signaling pathways. Although further investigation is needed to strengthen our findings, metabolomic data suggest that treated plants experienced a metabolic reprogramming following the application of the tested biostimulants. Nonetheless, our experimental data highlight the potential for combined use of high-throughput phenotyping and metabolomics to facilitate the screening of new substances with biostimulant properties and to provide a morpho-physiological and metabolomic gateway to the mechanisms underlying PHs action on plants.
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Foliar applications of a legume-Derived Protein hydrolysate elicit dose-dependent increases of growth, leaf mineral composition, yield and fruit quality in two greenhouse tomato cultivars.
Scientia Horticulturae, 2017Co-Authors: Youssef Rouphael, Giuseppe Colla, Maria Giordano, Christophe El-nakhel, Marios C. Kyriacou, Stefania De PascaleAbstract:Abstract The use of natural plant biostimulants is proposed as a promising and innovative approach to ensure improved and sustainable yields and product quality. A greenhouse experiment was performed to assess the yield performance, leaf net assimilation of CO 2 , mineral composition of leaves and fruits, and fruit physicochemical quality attributes of two tomato cultivars (Akyra and Sir Elyan) in relation to biostimulant treatments (control or two different concentrations of the legume-Derived Protein hydrolysate Trainer ® ). Treated tomato plants were sprayed every 10 days with a solution containing 2.5 and 5.0 ml L −1 of biostimulant. Akyra was found to be richest in K, Ca, Mg, lipophilic and hydrophilic antioxidant activities (LAA and HAA), lycopene, total phenolic and total ascorbic acid. Foliar applications of legume-Derived Protein hydrolysate at 5.0 ml L −1 increased marketable yield of Akyra and Sir Elyan by modulating yield components differently depending on cultivars: higher number of fruits in Akyra and increase of fruit mean weight in Sir Elyan. Improved yield performance with biostimulant foliar applications at the highest rate was related to improved leaf nutritional status (higher K and Mg) and higher net assimilation of CO 2 . The application of legume-Derived Protein hydrolysate at 5.0 ml L −1 , and to a lesser degree at 2.5 ml L −1 , elicited an increase in antioxidant activities, total soluble solids, mineral composition (K and Mg) as well as bioactive molecules such as lycopene and ascorbic acid, thereby increasing the nutritional and functional quality of the fruits. These findings can assist tomato growers in selecting cultivars and application dose for Protein hydrolysate to complement high crop productivity with optimal fruit quality.
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synergistic action of a microbial based biostimulant and a plant Derived Protein hydrolysate enhances lettuce tolerance to alkalinity and salinity
Frontiers in Plant Science, 2017Co-Authors: Youssef Rouphael, Mariateresa Cardarelli, Paolo Onini, Giuseppe CollaAbstract:In the coming years, farmers will have to deal with growing crops under suboptimal conditions dictated by global climate changes. The application of plant biostimulants such as beneficial microorganisms and plant-Derived Protein hydrolysates (PHs) may represent an interesting approach for increasing crop tolerance to alkalinity and salinity. The current research aimed at elucidating the agronomical, physiological, and biochemical effects as well as the changes in mineral composition of greenhouse lettuce (Lactuca sativa L.) either untreated or treated with a microbial-based biostimulant (Tablet) containing Rhizophagus intraradices and Trichoderma atroviride alone or in combination with a PH. Plants were sprayed with PH at weekly intervals with a solution containing 2.5 ml L-1 of PH. Lettuce plants were grown in sand culture and supplied with three nutrient solutions: standard, saline (25 mM NaCl) or alkaline (10 mM NaHCO3 + 0.5 g l-1 CaCO3; pH 8.1). Salt stress triggered a decrease in fresh yield, biomass production, SPAD index, chlorophyll fluorescence, leaf mineral composition and increased leaf proline concentration, without altering antioxidant enzyme activities. The decrease in marketable yield and biomass production under alkali stress was not significant. Irrespective of nutrient solution, the application of Tablet and especially Tablet + PH increased fresh marketable yield, shoot and root dry weight. This was associated with an improvement in SPAD index, Fv/Fm ratio, CAT and GPX activities and a better nutritional status (higher P, K and Fe and lower Na with NaCl and higher P and Fe with NaHCO3) via an increase of total root length and surface. The combination of microbial biostimulant with foliar application of PH synergistically increased the marketable fresh yield by 15.5% and 46.7% compared to the Tablet-treated and untreated plants, respectively. The improved crop performance of Tablet + PH application was attributed to a better root system architecture (higher total root length and surface), an improved chlorophyll synthesis and an increase in proline accumulation. Combined application of Tablet and PH could represent an effective strategy to minimize alkalinity and salinity stress in a sustainable way.
Mariateresa Cardarelli - One of the best experts on this subject based on the ideXlab platform.
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Understanding the Biostimulant Action of Vegetal-Derived Protein Hydrolysates by High-Throughput Plant Phenotyping and Metabolomics: A Case Study on Tomato
Frontiers Media S.A., 2019Co-Authors: Kenny Paul, Youssef Rouphael, Renaud Canaguier, Mariateresa Cardarelli, Mirella Sorrentino, Luigi Lucini, Paolo Bonini, Hélène Reynaud, Martin Trtílek, Klára PanzarováAbstract:Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective in finding new biostimulant substances. A screening protocol based on the use of high-throughput phenotyping platform for screening new vegetal-Derived Protein hydrolysates (PHs) for biostimulant activity followed by a metabolomic analysis to elucidate the mechanism of the most active PHs has been applied on tomato crop. Eight PHs (A–G, I) Derived from enzymatic hydrolysis of seed Proteins of Leguminosae and Brassicaceae species were foliarly sprayed twice during the trial. A non-ionic surfactant Triton X-100 at 0.1% was also added to the solutions before spraying. A control treatment foliarly sprayed with distilled water containing 0.1% Triton X-100 was also included. Untreated and PH-treated tomato plants were monitored regularly using high-throughput non-invasive imaging technologies. The phenotyping approach we used is based on automated integrative analysis of photosynthetic performance, growth analysis, and color index analysis. The digital biomass of the plants sprayed with PH was generally increased. In particular, the relative growth rate and the growth performance were significantly improved by PHs A and I, respectively, compared to the untreated control plants. Kinetic chlorophyll fluorescence imaging did not allow to differentiate the photosynthetic performance of treated and untreated plants. Finally, MS-based untargeted metabolomics analysis was performed in order to characterize the functional mechanisms of selected PHs. The treatment modulated the multi-layer regulation process that involved the ethylene precursor and polyamines and affected the ROS-mediated signaling pathways. Although further investigation is needed to strengthen our findings, metabolomic data suggest that treated plants experienced a metabolic reprogramming following the application of the tested biostimulants. Nonetheless, our experimental data highlight the potential for combined use of high-throughput phenotyping and metabolomics to facilitate the screening of new substances with biostimulant properties and to provide a morpho-physiological and metabolomic gateway to the mechanisms underlying PHs action on plants
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Table_4_Understanding the Biostimulant Action of Vegetal-Derived Protein Hydrolysates by High-Throughput Plant Phenotyping and Metabolomics: A Case Study on Tomato.DOCX
2019Co-Authors: Kenny Paul, Youssef Rouphael, Renaud Canaguier, Mariateresa Cardarelli, Mirella Sorrentino, Luigi Lucini, Paolo Bonini, Hélène Reynaud, Martin Trtílek, Klára PanzarováAbstract:Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective in finding new biostimulant substances. A screening protocol based on the use of high-throughput phenotyping platform for screening new vegetal-Derived Protein hydrolysates (PHs) for biostimulant activity followed by a metabolomic analysis to elucidate the mechanism of the most active PHs has been applied on tomato crop. Eight PHs (A–G, I) Derived from enzymatic hydrolysis of seed Proteins of Leguminosae and Brassicaceae species were foliarly sprayed twice during the trial. A non-ionic surfactant Triton X-100 at 0.1% was also added to the solutions before spraying. A control treatment foliarly sprayed with distilled water containing 0.1% Triton X-100 was also included. Untreated and PH-treated tomato plants were monitored regularly using high-throughput non-invasive imaging technologies. The phenotyping approach we used is based on automated integrative analysis of photosynthetic performance, growth analysis, and color index analysis. The digital biomass of the plants sprayed with PH was generally increased. In particular, the relative growth rate and the growth performance were significantly improved by PHs A and I, respectively, compared to the untreated control plants. Kinetic chlorophyll fluorescence imaging did not allow to differentiate the photosynthetic performance of treated and untreated plants. Finally, MS-based untargeted metabolomics analysis was performed in order to characterize the functional mechanisms of selected PHs. The treatment modulated the multi-layer regulation process that involved the ethylene precursor and polyamines and affected the ROS-mediated signaling pathways. Although further investigation is needed to strengthen our findings, metabolomic data suggest that treated plants experienced a metabolic reprogramming following the application of the tested biostimulants. Nonetheless, our experimental data highlight the potential for combined use of high-throughput phenotyping and metabolomics to facilitate the screening of new substances with biostimulant properties and to provide a morpho-physiological and metabolomic gateway to the mechanisms underlying PHs action on plants.
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Image_2_Understanding the Biostimulant Action of Vegetal-Derived Protein Hydrolysates by High-Throughput Plant Phenotyping and Metabolomics: A Case Study on Tomato.JPEG
2019Co-Authors: Kenny Paul, Youssef Rouphael, Renaud Canaguier, Mariateresa Cardarelli, Mirella Sorrentino, Luigi Lucini, Paolo Bonini, Hélène Reynaud, Martin Trtílek, Klára PanzarováAbstract:Designing and developing new biostimulants is a crucial process which requires an accurate testing of the product effects on the morpho-physiological traits of plants and a deep understanding of the mechanism of action of selected products. Product screening approaches using omics technologies have been found to be more efficient and cost effective in finding new biostimulant substances. A screening protocol based on the use of high-throughput phenotyping platform for screening new vegetal-Derived Protein hydrolysates (PHs) for biostimulant activity followed by a metabolomic analysis to elucidate the mechanism of the most active PHs has been applied on tomato crop. Eight PHs (A–G, I) Derived from enzymatic hydrolysis of seed Proteins of Leguminosae and Brassicaceae species were foliarly sprayed twice during the trial. A non-ionic surfactant Triton X-100 at 0.1% was also added to the solutions before spraying. A control treatment foliarly sprayed with distilled water containing 0.1% Triton X-100 was also included. Untreated and PH-treated tomato plants were monitored regularly using high-throughput non-invasive imaging technologies. The phenotyping approach we used is based on automated integrative analysis of photosynthetic performance, growth analysis, and color index analysis. The digital biomass of the plants sprayed with PH was generally increased. In particular, the relative growth rate and the growth performance were significantly improved by PHs A and I, respectively, compared to the untreated control plants. Kinetic chlorophyll fluorescence imaging did not allow to differentiate the photosynthetic performance of treated and untreated plants. Finally, MS-based untargeted metabolomics analysis was performed in order to characterize the functional mechanisms of selected PHs. The treatment modulated the multi-layer regulation process that involved the ethylene precursor and polyamines and affected the ROS-mediated signaling pathways. Although further investigation is needed to strengthen our findings, metabolomic data suggest that treated plants experienced a metabolic reprogramming following the application of the tested biostimulants. Nonetheless, our experimental data highlight the potential for combined use of high-throughput phenotyping and metabolomics to facilitate the screening of new substances with biostimulant properties and to provide a morpho-physiological and metabolomic gateway to the mechanisms underlying PHs action on plants.
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synergistic action of a microbial based biostimulant and a plant Derived Protein hydrolysate enhances lettuce tolerance to alkalinity and salinity
Frontiers in Plant Science, 2017Co-Authors: Youssef Rouphael, Mariateresa Cardarelli, Paolo Onini, Giuseppe CollaAbstract:In the coming years, farmers will have to deal with growing crops under suboptimal conditions dictated by global climate changes. The application of plant biostimulants such as beneficial microorganisms and plant-Derived Protein hydrolysates (PHs) may represent an interesting approach for increasing crop tolerance to alkalinity and salinity. The current research aimed at elucidating the agronomical, physiological, and biochemical effects as well as the changes in mineral composition of greenhouse lettuce (Lactuca sativa L.) either untreated or treated with a microbial-based biostimulant (Tablet) containing Rhizophagus intraradices and Trichoderma atroviride alone or in combination with a PH. Plants were sprayed with PH at weekly intervals with a solution containing 2.5 ml L-1 of PH. Lettuce plants were grown in sand culture and supplied with three nutrient solutions: standard, saline (25 mM NaCl) or alkaline (10 mM NaHCO3 + 0.5 g l-1 CaCO3; pH 8.1). Salt stress triggered a decrease in fresh yield, biomass production, SPAD index, chlorophyll fluorescence, leaf mineral composition and increased leaf proline concentration, without altering antioxidant enzyme activities. The decrease in marketable yield and biomass production under alkali stress was not significant. Irrespective of nutrient solution, the application of Tablet and especially Tablet + PH increased fresh marketable yield, shoot and root dry weight. This was associated with an improvement in SPAD index, Fv/Fm ratio, CAT and GPX activities and a better nutritional status (higher P, K and Fe and lower Na with NaCl and higher P and Fe with NaHCO3) via an increase of total root length and surface. The combination of microbial biostimulant with foliar application of PH synergistically increased the marketable fresh yield by 15.5% and 46.7% compared to the Tablet-treated and untreated plants, respectively. The improved crop performance of Tablet + PH application was attributed to a better root system architecture (higher total root length and surface), an improved chlorophyll synthesis and an increase in proline accumulation. Combined application of Tablet and PH could represent an effective strategy to minimize alkalinity and salinity stress in a sustainable way.
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the effect of a plant Derived biostimulant on metabolic profiling and crop performance of lettuce grown under saline conditions
Scientia Horticulturae, 2015Co-Authors: Luigi Lucini, Youssef Rouphael, Renaud Canaguier, Mariateresa Cardarelli, Pradeep Kumar, Giuseppe CollaAbstract:Abstract Plant-Derived Protein hydrolysates represent new biostimulant products able to improve crop tolerance to abiotic stresses. The aim of the study was to determine growth, root morphology, SPAD index, chlorophyll fluorescence, leaf mineral composition, and metabolic profiling of greenhouse lettuce either untreated or treated (root or leaf-root application) with a plant-Derived Protein hydrolysate. For foliar application, plants were sprayed with a solution containing 2.5 ml L −1 of biostimulant, whereas for root application, 100 mL of solution with the same concentration was applied to the growing medium at weekly intervals. Lettuce plants were supplied with two nutrient solutions: non-salt control (1 mM NaCl) or 25 mM NaCl. Salt stress decreased shoot and root dry biomass, SPAD index, chlorophyll fluorescence, leaf mineral composition and increased foliar proline concentration. Root and leaf-root application of the biostimulant increased fresh yield, dry biomass and root dry weight of lettuce under salinity conditions. This was associated with an improvement of plant nitrogen metabolism and an increase of the F v / F m -ratio efficiency in biostimulant-treated plants. Oxidative stress mitigation, increase in osmolytes, changes in sterols and terpenes composition, as well as the less expected increase in glucosinolates were also observed in biostimulant-treated plants grown under saline conditions. The present study proves that the application of plant-Derived Protein hydrolysate increases plant performance when plants are grown under salinity conditions. The most favorable metabolic profile was obtained when biostimulant was applied to both roots and leaves.