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P.r. Carnegie - One of the best experts on this subject based on the ideXlab platform.

  • Characterisation of an extracellular serine protease gene (nasp gene) from Dermatophilus congolensis
    FEMS microbiology letters, 2004
    Co-Authors: Alfredo Garcia-sanchez, Rosario Cerrato, J. Larrasa, Nicholas C Ambrose, A. Parra, J.m. Alonso, Miguel Hermoso-de-mendoza, Joaquín Rey, Madisa O Mine, P.r. Carnegie
    Abstract:

    A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases.

  • Characterisation of an extracellular serine protease gene (naspgene) from Dermatophilus congolensis
    2004
    Co-Authors: Alfredo Garcia-sanchez, Rosario Cerrato, J. Larrasa, Nicholas C Ambrose, A. Parra, J.m. Alonso, Miguel Hermoso-de-mendoza, Madisa O Mine, J.m. Rey, P.r. Carnegie
    Abstract:

    A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases. © 2004 Federation of European Microbiological Societies.

J.m. Alonso - One of the best experts on this subject based on the ideXlab platform.

  • Characterisation of an extracellular serine protease gene (nasp gene) from Dermatophilus congolensis
    FEMS microbiology letters, 2004
    Co-Authors: Alfredo Garcia-sanchez, Rosario Cerrato, J. Larrasa, Nicholas C Ambrose, A. Parra, J.m. Alonso, Miguel Hermoso-de-mendoza, Joaquín Rey, Madisa O Mine, P.r. Carnegie
    Abstract:

    A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases.

  • Characterisation of an extracellular serine protease gene (naspgene) from Dermatophilus congolensis
    2004
    Co-Authors: Alfredo Garcia-sanchez, Rosario Cerrato, J. Larrasa, Nicholas C Ambrose, A. Parra, J.m. Alonso, Miguel Hermoso-de-mendoza, Madisa O Mine, J.m. Rey, P.r. Carnegie
    Abstract:

    A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases. © 2004 Federation of European Microbiological Societies.

  • An indirect fluorescent antibody technique for detection of anti-Dermatophilus congolensis antibodies in sheep
    Tropical Animal Health and Production, 1994
    Co-Authors: J. Hermoso De Mendoza, J.m. Alonso, M. C. Gil, C. G. Nieto, A. Arenas, J. Rey, J. Anton, A. Cardenal, M. Hermoso De Mendoza
    Abstract:

    Se desarrolló una prueba indirecta de anticuerpos fluorescentes para detectar anticuerpos anti- Dermatophilus congolensis en ovejas. Se analizaron 25 sueros de ovejas clínicamente afectadas y bacteriológicamente positivas, y sueros de 10 corderos no afectados. Se utilizó un antigeno completo celular de D. congolensis cultivado en infusión de cerebro-corazón. Todos los sueros fueron analizados de la misma manera para reactividad cruzada, contra antígenos obtenidos de cultivos de Actinomyces viscosus, Micrococcus luteus, Nocardia asteroides y Corynebacterium pseudotuberculosis . Los sueros de ovejas infectadas con Dermatophilus , dieron resultados positivos con antígeno de D. congolensis y resultados negativos con antígenos de otras bacterias. Los antígenos completos celulares utilizados fueron simples de preparar y fáciles de reconocer micróscopicamente. La reactividad cruzada fue probada también utilizando el antígeno de D. congolensis y tres sueros provenientes de ovejas infectadas con Corynebacterium pseudotuberculosis, Actinomyces pyogenes y Nocardia asteroides . Ninguno de los sueros reaccionó positivamente. Los autores recomiendan la técnica para estudios serológicos e investigación sobre dermatofilosis. An indirect fluorescent antibody (IFA) technique has been developed for detection of anti- Dermatophilus antibodies in sheep. Sera from 25 bacteriologically confirmed clinically affected sheep and from 10 negative non affected lambs were used. Whole cell antigen from brain heart infusion cultures of D. congolensis was used and all sera were tested in the same way for cross-reactivity against antigens obtained from cultures of Actinomyces viscosus, Micrococcus luteus, Nocardia asteroides , and Corynebacterium pseudotuberculosis . Sera from Dermatophilus -infected sheep gave positive results with D. congolensis antigen and negative results with the antigens from other bacteria. The whole cell antigens employed were simple to prepare and easy to recognise by microscopy. Cross-reactivity was further tested using the D. congolensis culture whole cell antigen and 3 sera from sheep with bacteriologically confirmed natural infections due to Corynebacterium pseudotuberculosis, Actinomyces pyogenes and Nocardia asteroides . None of these sera showed positive reactions. The authors recommend this technique for serological surveys and research on dermatophilosis. Une technique d'immunofluorescence indirecte a été mise au point pour déceler des anticorps anti- Dermatophilus chez le mouton. Des sérums ont été prélevés chez 25 moutons reconnus malades après examen bactériologique et chez 10 agneaux indemnes. Un antigène (de corps bactérien) obtenu par culture de D. congolensis en bouillon cerveau-coeur a été utilisé. Tous les sérums ont été testés de façon identique pour la recherche de réaction croisée contre des antigènes obtenus à partir de cultures de Actinomyces viscosus, Micrococcus luteus, Nocardia asteroides et Corynebacterium pseudotuberculosis . Les sérums de moutons infectés par D. congolensis ont donné des résultats positifs avec l'antigène D. congolensis et négatifs avec les autres antigènes. La préparation des antigènes bactériens était simple et leur reconnaissance au microscope aisée. La recherche des réactions croisées a été poursuivie en utilisant l'antigène bactérien de D. congolensis contre 3 sérums de moutons naturellement infectés par Corynebacterium pseudotuberculosis, Actinomyces pyogenes et Nocardia asteroides . Autun de ces sérums ne s'est rélévé positif. Les auteurs recommandent cette technique pour les enquêtes sérologiques et la recherche sur la dermatophilose.

  • Enzymatic activities of Dermatophilus congolensis measured by API ZYM
    Veterinary microbiology, 1993
    Co-Authors: J. Hermoso De Mendoza, J.m. Alonso, Joaquín Rey, Antonio Arenas, M. C. Gil, J.m. Anton, M. Hermoso De Mendoza
    Abstract:

    API ZYM kit was used to test enzymatic activities on eighteen strains of Dermatophilus congolensis. All strains produced lipase and acid phosphatase, which act on lipids, and leucine arylamidase which act on proteins. Another 10 exoenzymes were present in at least one of the strains.

Alfredo Garcia-sanchez - One of the best experts on this subject based on the ideXlab platform.

  • Characterisation of an extracellular serine protease gene (nasp gene) from Dermatophilus congolensis
    FEMS microbiology letters, 2004
    Co-Authors: Alfredo Garcia-sanchez, Rosario Cerrato, J. Larrasa, Nicholas C Ambrose, A. Parra, J.m. Alonso, Miguel Hermoso-de-mendoza, Joaquín Rey, Madisa O Mine, P.r. Carnegie
    Abstract:

    A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases.

  • Characterisation of an extracellular serine protease gene (naspgene) from Dermatophilus congolensis
    2004
    Co-Authors: Alfredo Garcia-sanchez, Rosario Cerrato, J. Larrasa, Nicholas C Ambrose, A. Parra, J.m. Alonso, Miguel Hermoso-de-mendoza, Madisa O Mine, J.m. Rey, P.r. Carnegie
    Abstract:

    A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases. © 2004 Federation of European Microbiological Societies.

Joaquín Rey - One of the best experts on this subject based on the ideXlab platform.

  • Characterisation of an extracellular serine protease gene (nasp gene) from Dermatophilus congolensis
    FEMS microbiology letters, 2004
    Co-Authors: Alfredo Garcia-sanchez, Rosario Cerrato, J. Larrasa, Nicholas C Ambrose, A. Parra, J.m. Alonso, Miguel Hermoso-de-mendoza, Joaquín Rey, Madisa O Mine, P.r. Carnegie
    Abstract:

    A partial amino acid sequence of a serine protease from Dermatophilus congolensis allowed the design of oligonucleotide primers that were complemented with additional ones from previously published partial sequences of the gene encoding the enzyme. The polymerase chain reaction (PCR), using combinations of specific and degenerate oligonucleotide primers, allowed the amplification of a 1738-bp internal fragment of the gene, which was finally characterised by inverse PCR as the first full-length sequenced serine protease gene (nasp) from Dermatophilus congolensis. The deduced amino acid sequence of this enzyme, probably involved in the pathogenesis of dermatophilosis, links it to the subtilisin family of proteases.

  • Enzymatic activities of Dermatophilus congolensis measured by API ZYM
    Veterinary microbiology, 1993
    Co-Authors: J. Hermoso De Mendoza, J.m. Alonso, Joaquín Rey, Antonio Arenas, M. C. Gil, J.m. Anton, M. Hermoso De Mendoza
    Abstract:

    API ZYM kit was used to test enzymatic activities on eighteen strains of Dermatophilus congolensis. All strains produced lipase and acid phosphatase, which act on lipids, and leucine arylamidase which act on proteins. Another 10 exoenzymes were present in at least one of the strains.

Daniel Martineau - One of the best experts on this subject based on the ideXlab platform.

  • Dermatophilus‐like infection in beluga whales, Delphinapterus leucas, from the St. Lawrence estuary
    Veterinary dermatology, 2001
    Co-Authors: I. Mikaelian, Jean-martin Lapointe, P. Labelle, Robert Higgins, Manon Paradis, Daniel Martineau
    Abstract:

    Six beluga whales (Delphinapterus leucas) found dead on the shores of the St. Lawrence estuary had multiple slightly depressed greyish round areas randomly distributed over the whole body. Histologically, the surface of these areas was covered with a thick layer of Dermatophilus-like organisms which invaded the stratum corneum. The underlying stratum spinosum had marked spongiosis and vacuolar degeneration. Minimal neutrophilic infiltration was present within the underlying dermal papillae. To the authors' knowledge, dermatophilosis in cetaceans has not been reported previously.

  • Dermatophilus like infection in beluga whales delphinapterus leucas from the st lawrence estuary
    Veterinary Dermatology, 2001
    Co-Authors: I. Mikaelian, Jean-martin Lapointe, P. Labelle, Robert Higgins, Manon Paradis, Daniel Martineau
    Abstract:

    Six beluga whales (Delphinapterus leucas) found dead on the shores of the St. Lawrence estuary had multiple slightly depressed greyish round areas randomly distributed over the whole body. Histologically, the surface of these areas was covered with a thick layer of Dermatophilus-like organisms which invaded the stratum corneum. The underlying stratum spinosum had marked spongiosis and vacuolar degeneration. Minimal neutrophilic infiltration was present within the underlying dermal papillae. To the authors' knowledge, dermatophilosis in cetaceans has not been reported previously.