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Bernard Ollivier - One of the best experts on this subject based on the ideXlab platform.

  • Desulfotomaculum aquiferis sp nov and Desulfotomaculum profundi sp nov., isolated from a deep natural gas storage aquifer
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: Sabrina Berlendis, Marielaure Fardeau, Bernard Ollivier, Manon Joseph, Michel Magot, Jean-francois Lascourreges, David Dequidt, Magali Ranchou-peyruse, Thomas Auello, Anthony Ranchou-peyruse
    Abstract:

    Two novel strictly anaerobic bacteria, strains Bs105(T) and Bs107(T), were isolated from a deep aquifer-derived hydrocarbonoclastic community. The cells were rod-shaped, not motile and had terminal spores. Phylogenetic affiliation and physiological properties revealed that these isolates belong to two novel species of the genus Desulfotomaculum. Optimal growth temperatures for strains Bs105(T) and Bs107(T) were 42 and 45 degrees C, respectively. The estimated G+C content of the genomic DNA was 42.9 and 48.7 mol%. For both strains, the major cellular fatty acid was palmitate (C-16 : 0). Specific carbon fatty acid signatures of Gram-positive bacteria (iso-C-17 : 0) and sulfate-reducing bacteria (C(17 : 0)cyc) were also detected. An insertion was revealed in one of the two 16S rRNA gene copies harboured by strain Bs107(T). Similar insertions have previously been highlighted among moderately thermophilic species of the genus Desulfotomaculum. Both strains shared the ability to oxidize aromatic acids (Bs105(T) : hydroquinone, acetophenone, paratoluic acid, 2-phenylethanol, trans-cinnamic acid, 4-hydroxybenzaldehyde, benzyl alcohol, benzoic acid 4-hydroxybutyl ester; Bs107(T) : ortho-toluic acid, benzoic acid 4-hydroxybutyl ester). The names Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov. are proposed for the type strains Bs105(T) (= DSM 24088(T) = JCM 31386(T)) and Bs107(T) (= DSM 24093(T) = JCM 31387(T)).

  • Desulfotomaculum aquiferis sp nov and Desulfotomaculum profundi sp nov isolated from a deep natural gas storage aquifer
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: Marielaure Fardeau, Bernard Ollivier, Manon Joseph, Thomas Aüllo, Sabrina Berlendis, Jean-francois Lascourreges, Magali Ranchoupeyruse, David Dequidt, Michel Magot
    Abstract:

    Two novel strictly anaerobic bacteria, strains Bs105T and Bs107T, were isolated from a deep aquifer-derived hydrocarbonoclastic community. The cells were rod-shaped, not motile and had terminal spores. Phylogenetic affiliation and physiological properties revealed that these isolates belong to two novel species of the genus Desulfotomaculum . Optimal growth temperatures for strains Bs105T and Bs107T were 42 and 45 °C, respectively. The estimated G+C content of the genomic DNA was 42.9 and 48.7 mol%. For both strains, the major cellular fatty acid was palmitate (C16 : 0). Specific carbon fatty acid signatures of Gram-positive bacteria (iso-C17 : 0) and sulfate-reducing bacteria (C17 : 0 cyc) were also detected. An insertion was revealed in one of the two 16S rRNA gene copies harboured by strain Bs107T. Similar insertions have previously been highlighted among moderately thermophilic species of the genus Desulfotomaculum . Both strains shared the ability to oxidize aromatic acids (Bs105T: hydroquinone, acetophenone, para-toluic acid, 2-phenylethanol, trans-cinnamic acid, 4-hydroxybenzaldehyde, benzyl alcohol, benzoic acid 4-hydroxybutyl ester; Bs107T: ortho-toluic acid, benzoic acid 4-hydroxybutyl ester). The names Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov. are proposed for the type strains Bs105T (=DSM 24088T=JCM 31386T) and Bs107T (=DSM 24093T=JCM 31387T).

  • Desulfotomaculum spp. and related gram-positive sulfate-reducing bacteria in deep subsurface environments.
    Frontiers in microbiology, 2013
    Co-Authors: Thomas Aüllo, Bernard Ollivier, Anthony Ranchou-peyruse, Michel Magot
    Abstract:

    Gram-positive spore-forming sulfate reducers and particularly members of the genus Desulfotomaculum are commonly found in the subsurface biosphere by culture based and molecular approaches. Due to their metabolic versatility and their ability to persist as endospores. Desulfotomaculum spp. are well-adapted for colonizing environments through a slow sedimentation process. Because of their ability to grow autotrophically (H2/CO2) and produce sulfide or acetate, these microorganisms may play key roles in deep lithoautotrophic microbial communities. Available data about Desulfotomaculum spp. and related species from studies carried out from deep freshwater lakes, marine sediments, oligotrophic and organic rich deep geological settings are discussed in this review.

  • Desulfotomaculum peckii sp. nov., a moderately thermophilic member of the genus Desulfotomaculum , isolated from an upflow anaerobic filter treating abattoir wastewaters
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Linda Jabari, Guy Fauque, Bernard Ollivier, Jean-luc Cayol, Moktar Hamdi, Hana Gannoun, Marielaure Fardeau
    Abstract:

    A novel anaerobic thermophilic sulfate-reducing bacterium designated strain LINDBHT1T was isolated from an anaerobic digester treating abattoir wastewaters in Tunisia. Strain LINDBHT1T grew at temperatures between 50 and 65 °C (optimum 55–60 °C), and at pH between 5.9 and 9.2 (optimum pH 6.0–6.8). Strain LINDBHT1T required salt for growth (1–40 g NaCl l−1), with an optimum of 20–30 g l−1. In the presence of sulfate as terminal electron acceptor, strain LINDBHT1T used H2/CO2, propanol, butanol and ethanol as carbon and energy sources but fumarate, formate, lactate and pyruvate were not utilized. Butanol was converted to butyrate, while propanol and ethanol were oxidized to propionate and acetate, respectively. Sulfate, sulfite and thiosulfate were utilized as terminal electron acceptors but elemental sulfur, iron (III), fumarate, nitrate and nitrite were not used. The G+C content of the genomic DNA was 44.4 mol%. Phylogenetic analysis of the small-subunit rRNA gene sequence indicated that strain LINDBHT1T was affiliated to the genus Desulfotomaculum with the type strains of Desulfotomaculum halophilum and Desulfotomaculum alkaliphilum as its closest phylogenetic relatives (about 89 % similarity). This strain represents a novel species of the genus Desulfotomaculum , Desulfotomaculum peckii sp. nov.; the type strain is LINDBHT1T ( = DSM 23769T = JCM 17209T).

  • Desulfotomaculum peckii sp. nov., a moderately thermophilic member of the genus Desulfotomaculum, isolated from an upflow anaerobic filter treating abattoir wastewaters.
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Linda Jabari, Guy Fauque, Bernard Ollivier, Jean-luc Cayol, Moktar Hamdi, Hana Gannoun, Marielaure Fardeau
    Abstract:

    A novel anaerobic thermophilic sulfate-reducing bacterium designated strain LINDBHT1(T) was isolated from an anaerobic digester treating abattoir wastewaters in Tunisia. Strain LINDBHT1(T) grew at temperatures between 50 and 65 °C (optimum 55-60 °C), and at pH between 5.9 and 9.2 (optimum pH 6.0-6.8). Strain LINDBHT1(T) required salt for growth (1-40 g NaCl l(-1)), with an optimum of 20-30 g l(-1). In the presence of sulfate as terminal electron acceptor, strain LINDBHT1(T) used H2/CO2, propanol, butanol and ethanol as carbon and energy sources but fumarate, formate, lactate and pyruvate were not utilized. Butanol was converted to butyrate, while propanol and ethanol were oxidized to propionate and acetate, respectively. Sulfate, sulfite and thiosulfate were utilized as terminal electron acceptors but elemental sulfur, iron (III), fumarate, nitrate and nitrite were not used. The G+C content of the genomic DNA was 44.4 mol%. Phylogenetic analysis of the small-subunit rRNA gene sequence indicated that strain LINDBHT1(T) was affiliated to the genus Desulfotomaculum with the type strains of Desulfotomaculum halophilum and Desulfotomaculum alkaliphilum as its closest phylogenetic relatives (about 89 % similarity). This strain represents a novel species of the genus Desulfotomaculum, Desulfotomaculum peckii sp. nov.; the type strain is LINDBHT1(T) ( = DSM 23769(T) = JCM 17209(T)).

Marielaure Fardeau - One of the best experts on this subject based on the ideXlab platform.

  • Desulfotomaculum aquiferis sp nov and Desulfotomaculum profundi sp nov isolated from a deep natural gas storage aquifer
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: Marielaure Fardeau, Bernard Ollivier, Manon Joseph, Thomas Aüllo, Sabrina Berlendis, Jean-francois Lascourreges, Magali Ranchoupeyruse, David Dequidt, Michel Magot
    Abstract:

    Two novel strictly anaerobic bacteria, strains Bs105T and Bs107T, were isolated from a deep aquifer-derived hydrocarbonoclastic community. The cells were rod-shaped, not motile and had terminal spores. Phylogenetic affiliation and physiological properties revealed that these isolates belong to two novel species of the genus Desulfotomaculum . Optimal growth temperatures for strains Bs105T and Bs107T were 42 and 45 °C, respectively. The estimated G+C content of the genomic DNA was 42.9 and 48.7 mol%. For both strains, the major cellular fatty acid was palmitate (C16 : 0). Specific carbon fatty acid signatures of Gram-positive bacteria (iso-C17 : 0) and sulfate-reducing bacteria (C17 : 0 cyc) were also detected. An insertion was revealed in one of the two 16S rRNA gene copies harboured by strain Bs107T. Similar insertions have previously been highlighted among moderately thermophilic species of the genus Desulfotomaculum . Both strains shared the ability to oxidize aromatic acids (Bs105T: hydroquinone, acetophenone, para-toluic acid, 2-phenylethanol, trans-cinnamic acid, 4-hydroxybenzaldehyde, benzyl alcohol, benzoic acid 4-hydroxybutyl ester; Bs107T: ortho-toluic acid, benzoic acid 4-hydroxybutyl ester). The names Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov. are proposed for the type strains Bs105T (=DSM 24088T=JCM 31386T) and Bs107T (=DSM 24093T=JCM 31387T).

  • Desulfotomaculum aquiferis sp nov and Desulfotomaculum profundi sp nov., isolated from a deep natural gas storage aquifer
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: Sabrina Berlendis, Marielaure Fardeau, Bernard Ollivier, Manon Joseph, Michel Magot, Jean-francois Lascourreges, David Dequidt, Magali Ranchou-peyruse, Thomas Auello, Anthony Ranchou-peyruse
    Abstract:

    Two novel strictly anaerobic bacteria, strains Bs105(T) and Bs107(T), were isolated from a deep aquifer-derived hydrocarbonoclastic community. The cells were rod-shaped, not motile and had terminal spores. Phylogenetic affiliation and physiological properties revealed that these isolates belong to two novel species of the genus Desulfotomaculum. Optimal growth temperatures for strains Bs105(T) and Bs107(T) were 42 and 45 degrees C, respectively. The estimated G+C content of the genomic DNA was 42.9 and 48.7 mol%. For both strains, the major cellular fatty acid was palmitate (C-16 : 0). Specific carbon fatty acid signatures of Gram-positive bacteria (iso-C-17 : 0) and sulfate-reducing bacteria (C(17 : 0)cyc) were also detected. An insertion was revealed in one of the two 16S rRNA gene copies harboured by strain Bs107(T). Similar insertions have previously been highlighted among moderately thermophilic species of the genus Desulfotomaculum. Both strains shared the ability to oxidize aromatic acids (Bs105(T) : hydroquinone, acetophenone, paratoluic acid, 2-phenylethanol, trans-cinnamic acid, 4-hydroxybenzaldehyde, benzyl alcohol, benzoic acid 4-hydroxybutyl ester; Bs107(T) : ortho-toluic acid, benzoic acid 4-hydroxybutyl ester). The names Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov. are proposed for the type strains Bs105(T) (= DSM 24088(T) = JCM 31386(T)) and Bs107(T) (= DSM 24093(T) = JCM 31387(T)).

  • Desulfotomaculum peckii sp. nov., a moderately thermophilic member of the genus Desulfotomaculum , isolated from an upflow anaerobic filter treating abattoir wastewaters
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Linda Jabari, Guy Fauque, Bernard Ollivier, Jean-luc Cayol, Moktar Hamdi, Hana Gannoun, Marielaure Fardeau
    Abstract:

    A novel anaerobic thermophilic sulfate-reducing bacterium designated strain LINDBHT1T was isolated from an anaerobic digester treating abattoir wastewaters in Tunisia. Strain LINDBHT1T grew at temperatures between 50 and 65 °C (optimum 55–60 °C), and at pH between 5.9 and 9.2 (optimum pH 6.0–6.8). Strain LINDBHT1T required salt for growth (1–40 g NaCl l−1), with an optimum of 20–30 g l−1. In the presence of sulfate as terminal electron acceptor, strain LINDBHT1T used H2/CO2, propanol, butanol and ethanol as carbon and energy sources but fumarate, formate, lactate and pyruvate were not utilized. Butanol was converted to butyrate, while propanol and ethanol were oxidized to propionate and acetate, respectively. Sulfate, sulfite and thiosulfate were utilized as terminal electron acceptors but elemental sulfur, iron (III), fumarate, nitrate and nitrite were not used. The G+C content of the genomic DNA was 44.4 mol%. Phylogenetic analysis of the small-subunit rRNA gene sequence indicated that strain LINDBHT1T was affiliated to the genus Desulfotomaculum with the type strains of Desulfotomaculum halophilum and Desulfotomaculum alkaliphilum as its closest phylogenetic relatives (about 89 % similarity). This strain represents a novel species of the genus Desulfotomaculum , Desulfotomaculum peckii sp. nov.; the type strain is LINDBHT1T ( = DSM 23769T = JCM 17209T).

  • Desulfotomaculum peckii sp. nov., a moderately thermophilic member of the genus Desulfotomaculum, isolated from an upflow anaerobic filter treating abattoir wastewaters.
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Linda Jabari, Guy Fauque, Bernard Ollivier, Jean-luc Cayol, Moktar Hamdi, Hana Gannoun, Marielaure Fardeau
    Abstract:

    A novel anaerobic thermophilic sulfate-reducing bacterium designated strain LINDBHT1(T) was isolated from an anaerobic digester treating abattoir wastewaters in Tunisia. Strain LINDBHT1(T) grew at temperatures between 50 and 65 °C (optimum 55-60 °C), and at pH between 5.9 and 9.2 (optimum pH 6.0-6.8). Strain LINDBHT1(T) required salt for growth (1-40 g NaCl l(-1)), with an optimum of 20-30 g l(-1). In the presence of sulfate as terminal electron acceptor, strain LINDBHT1(T) used H2/CO2, propanol, butanol and ethanol as carbon and energy sources but fumarate, formate, lactate and pyruvate were not utilized. Butanol was converted to butyrate, while propanol and ethanol were oxidized to propionate and acetate, respectively. Sulfate, sulfite and thiosulfate were utilized as terminal electron acceptors but elemental sulfur, iron (III), fumarate, nitrate and nitrite were not used. The G+C content of the genomic DNA was 44.4 mol%. Phylogenetic analysis of the small-subunit rRNA gene sequence indicated that strain LINDBHT1(T) was affiliated to the genus Desulfotomaculum with the type strains of Desulfotomaculum halophilum and Desulfotomaculum alkaliphilum as its closest phylogenetic relatives (about 89 % similarity). This strain represents a novel species of the genus Desulfotomaculum, Desulfotomaculum peckii sp. nov.; the type strain is LINDBHT1(T) ( = DSM 23769(T) = JCM 17209(T)).

  • Genome Sequence of the Sulfate-Reducing Bacterium Desulfotomaculum hydrothermale Lam5(T).
    Genome announcements, 2013
    Co-Authors: Oulfat Amin, Marielaure Fardeau, Bernard Ollivier, Odile Valette, Agnès Hirschler-réa, Valérie Barbe, Claudine Médigue, Benoit Vacherie, Philippe N. Bertin, Alain Dolla
    Abstract:

    Here, we report the draft genome sequence of Desulfotomaculum hydrothermale, a sulfate-reducing, spore-forming bacterium isolated from a Tunisian hot spring. The genome is composed of 2.7 Mb, with a G+C content of 49.48%, and it contains 2,643 protein-coding sequences.

Jaakko A Puhakka - One of the best experts on this subject based on the ideXlab platform.

  • Desulfotomaculum alcoholivorax sp nov a moderately thermophilic spore forming sulfate reducer isolated from a fluidized bed reactor treating acidic metal and sulfate containing wastewater
    International Journal of Systematic and Evolutionary Microbiology, 2008
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33–51 °C (optimum 44–46 °C) and the pH range was 6.0–7.5 (optimum pH 6.4–7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).

  • Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater.
    International journal of systematic and evolutionary microbiology, 2008
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33-51 degrees C (optimum 44-46 degrees C) and the pH range was 6.0-7.5 (optimum pH 6.4-7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 omega 10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).

  • Desulfotomaculum thermosubterraneum sp nov a thermophilic sulfate reducer isolated from an underground mine located in a geothermally active area
    International Journal of Systematic and Evolutionary Microbiology, 2006
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50–72 °C (optimum growth at 61–66 °C) and the pH range was 6.4–7.8 (optimum at pH 7.2–7.4). Strain RL50JIIIT tolerated up to 1.5 % NaCl, but optimum growth occurred in the presence of 0–1 % NaCl. Electron acceptors utilized were sulfate, sulfite, thiosulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 0 DMA (dimethyl acetal), iso-C15 : 0 DMA and iso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7 % similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA–DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).

  • novel thermophilic sulfate reducing bacteria from a geothermally active underground mine in japan
    Applied and Environmental Microbiology, 2006
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Jason J Plumb, W J Robertson, Peter D Franzmann, Jaakko A Puhakka
    Abstract:

    Thermophilic sulfate-reducing bacteria were enriched from samples obtained from a geothermal underground mine in Japan. The enrichment cultures contained bacteria affiliated with the genera Desulfotomaculum, Thermanaeromonas, Thermincola, Thermovenabulum, Moorella, “Natronoanaerobium,” and Clostridium. Two novel thermophilic sulfate-reducing strains, RL50JIII and RL80JIV, affiliated with the genera Desulfotomaculum and Thermanaeromonas, respectively, were isolated.

  • Desulfotomaculum thermosubterraneum sp. nov., a thermophilic sulfate-reducer isolated from an underground mine located in a geothermally active area.
    International journal of systematic and evolutionary microbiology, 2006
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50-72 degrees C (optimum growth at 61-66 degrees C) and the pH range was 6.4-7.8 (optimum at pH 7.2-7.4). Strain RL50JIIIT tolerated up to 1.5% NaCl, but optimum growth occurred in the presence of 0-1% NaCl. Electron acceptors utilized were sulfate, sulfite, thiosulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15:0, iso-C17:0 DMA (dimethyl acetal), iso-C15:0 DMA and iso-C17:0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7% similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA-DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).

Stefan Spring - One of the best experts on this subject based on the ideXlab platform.

  • Standards in Genomic Sciences (2012) 7:304-319 DOI:10.4056/sigs.3226659 The Genomic Standards Consortium
    2016
    Co-Authors: Stefan Spring, Michael Visser, Alla Lapidus, Susan Lucas, Jan Fang Cheng, Cliff Han, Roxanne Tapia, Alex Copel, Lynne A. Goodwin
    Abstract:

    Complete genome sequence of the sulfate-reducing firmicute Desulfotomaculum ruminis type strain (DLT

  • Desulfotomaculum defluvii sp. nov., a sulfate-reducing bacterium isolated from the subsurface environment of a landfill
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Srinivasan Krishnamurthi, Stefan Spring, Pinnaka Anil Kumar, Shanmugam Mayilraj, Hans-peter Klenk, Korpole Suresh
    Abstract:

    A novel sulfate-reducing, strictly anaerobic and endospore-forming bacterium, designated strain A5LFS102T, was isolated from a subsurface landfill sample. The strain was characterized using a polyphasic approach. Optimal growth was observed at 37 °C and pH 7.5 with sulfate as an electron acceptor. Sulfite and thiosulfate were utilized as electron acceptors. The respiratory isoprenoid quinone was menaquinone MK-7. 16S rRNA gene sequence analysis assigned strain A5LFS102T to the genus Desulfotomaculum . Both 16S rRNA and dissimilatory sulfate reductase (dsr) genes were compared with those of representative members of the genus Desulfotomaculum . Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A5LFS102T was closely related to Desulfotomaculum aeronauticum DSM 10349T (94.6 % sequence similarity). The G+C content of the DNA was 45.4 mol%. The total cellular fatty acid profile was dominated by C16 fatty acids. These phenotypic and genotypic data showed that strain A5LFS102T should be recognized as representative of a novel species of the genus Desulfotomaculum , for which the name Desulfotomaculum defluvii sp. nov. is proposed. The type strain is A5LFS102T ( = DSM 23699T = JCM 14036T = MTCC 7767T).

  • Complete genome sequence of the sulfate-reducing firmicute Desulfotomaculum ruminis type strain (DLT)
    Standards in genomic sciences, 2012
    Co-Authors: Stefan Spring, Michael Visser, Alex Copeland, Alla Lapidus, Susan Lucas, Jan Fang Cheng, Cliff Han, Roxanne Tapia, Lynne Goodwin
    Abstract:

    Desulfotomaculum ruminis Campbell and Postgate 1965 is a member of the large genus Desulfotomaculum which contains 30 species and is contained in the family Peptococcaceae. This species is of interest because it represents one of the few sulfate-reducing bacteria that have been isolated from the rumen. Here we describe the features of D. ruminis together with the complete genome sequence and annotation. The 3,969,014 bp long chromosome with a total of 3,901 protein-coding and 85 RNA genes is the second completed genome sequence of a type strain of the genus Desulfotomaculum to be published, and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2009.

  • Complete genome sequence of Desulfotomaculum acetoxidans type strain (5575^T)
    Standards in Genomic Sciences, 2009
    Co-Authors: Stefan Spring, Alex Copeland, Alla Lapidus, Maren Schröder, Dorothea Gleim, David Sims, Linda Meincke, Tijana Glavina Del Rio, Hope Tice, Jan Fang Cheng
    Abstract:

    Desulfotomaculum acetoxidans Widdel and Pfennig 1977 was one of the first sulfate-reducing bacteria known to grow with acetate as sole energy and carbon source. It is able to oxidize substrates completely to carbon dioxide with sulfate as the electron acceptor, which is reduced to hydrogen sulfide. All available data about this species are based on strain 5575^T, isolated from piggery waste in Germany. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of a Desulfotomaculum species with validly published name. The 4,545,624 bp long single replicon genome with its 4370 protein-coding and 100 RNA genes is a part of the G enomic E ncyclopedia of B acteria and A rchaea project.

  • Desulfotomaculum alcoholivorax sp nov a moderately thermophilic spore forming sulfate reducer isolated from a fluidized bed reactor treating acidic metal and sulfate containing wastewater
    International Journal of Systematic and Evolutionary Microbiology, 2008
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33–51 °C (optimum 44–46 °C) and the pH range was 6.0–7.5 (optimum pH 6.4–7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).

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  • Desulfotomaculum alcoholivorax sp nov a moderately thermophilic spore forming sulfate reducer isolated from a fluidized bed reactor treating acidic metal and sulfate containing wastewater
    International Journal of Systematic and Evolutionary Microbiology, 2008
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33–51 °C (optimum 44–46 °C) and the pH range was 6.0–7.5 (optimum pH 6.4–7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).

  • Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater.
    International journal of systematic and evolutionary microbiology, 2008
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33-51 degrees C (optimum 44-46 degrees C) and the pH range was 6.0-7.5 (optimum pH 6.4-7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 omega 10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).

  • Desulfotomaculum thermosubterraneum sp nov a thermophilic sulfate reducer isolated from an underground mine located in a geothermally active area
    International Journal of Systematic and Evolutionary Microbiology, 2006
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50–72 °C (optimum growth at 61–66 °C) and the pH range was 6.4–7.8 (optimum at pH 7.2–7.4). Strain RL50JIIIT tolerated up to 1.5 % NaCl, but optimum growth occurred in the presence of 0–1 % NaCl. Electron acceptors utilized were sulfate, sulfite, thiosulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 0 DMA (dimethyl acetal), iso-C15 : 0 DMA and iso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7 % similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA–DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).

  • novel thermophilic sulfate reducing bacteria from a geothermally active underground mine in japan
    Applied and Environmental Microbiology, 2006
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Jason J Plumb, W J Robertson, Peter D Franzmann, Jaakko A Puhakka
    Abstract:

    Thermophilic sulfate-reducing bacteria were enriched from samples obtained from a geothermal underground mine in Japan. The enrichment cultures contained bacteria affiliated with the genera Desulfotomaculum, Thermanaeromonas, Thermincola, Thermovenabulum, Moorella, “Natronoanaerobium,” and Clostridium. Two novel thermophilic sulfate-reducing strains, RL50JIII and RL80JIV, affiliated with the genera Desulfotomaculum and Thermanaeromonas, respectively, were isolated.

  • Desulfotomaculum thermosubterraneum sp. nov., a thermophilic sulfate-reducer isolated from an underground mine located in a geothermally active area.
    International journal of systematic and evolutionary microbiology, 2006
    Co-Authors: Anna H Kaksonen, Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, Jaakko A Puhakka
    Abstract:

    A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50-72 degrees C (optimum growth at 61-66 degrees C) and the pH range was 6.4-7.8 (optimum at pH 7.2-7.4). Strain RL50JIIIT tolerated up to 1.5% NaCl, but optimum growth occurred in the presence of 0-1% NaCl. Electron acceptors utilized were sulfate, sulfite, thiosulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15:0, iso-C17:0 DMA (dimethyl acetal), iso-C15:0 DMA and iso-C17:0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7% similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA-DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).