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Koji Koyama - One of the best experts on this subject based on the ideXlab platform.

  • Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in vitro fertilization.
    Fertility and sterility, 2006
    Co-Authors: Akiko Hasegawa, Nahoko Mochida, Toshitada Ogasawara, Koji Koyama
    Abstract:

    To determine an optimum condition for vitrification of various animal species and to examine the developing capacity into pups of mouse oocytes in preantral follicles originating from cryopreserved ovaries. Experimental animal study. Laboratory environment. Normal (C57BL/6 x DBA2) F1 mice. Vitrification of animal ovaries using polyester sheets as a storage Device; Collection of oocyte-granulosa cell complexes by enzymatic treatment; and in vitro growth (IVG), in vitro maturation (IVM), and IVF, with embryo transplantation to pseudopregnant mice. Histological analysis of vitrified and warmed ovaries from several animal species; measurement of successful rates in IVG, IVM, and IVF of mouse oocytes in oocyte-granulosa cell complexes collected from the vitrified and warmed ovaries; and achievement of pup birth. The vitrification method used was effective for storage of various animal ovaries. The oocytes enclosed in preantral follicles that were yielded from the vitrified-warmed ovaries preserved the capacity for developing into pups after IVG, IVM, and IVF. The findings indicated that ovarian cryopreservation by vitrification and subsequent IVG, IVM, and IVF are promising methods for restoring young cancer patients' fertility. The practical information presented here is applicable to human ovarian tissues.

  • Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in vitro fertilization.
    Fertility and Sterility, 2006
    Co-Authors: Akiko Hasegawa, Nahoko Mochida, Toshitada Ogasawara, Koji Koyama
    Abstract:

    Objective To determine an optimum condition for vitrification of various animal species and to examine the developing capacity into pups of mouse oocytes in preantral follicles originating from cryopreserved ovaries. Design Experimental animal study. Setting Laboratory environment. Animal(s) Normal (C57BL/6 × DBA2) F1 mice. Intervention(s) Vitrification of animal ovaries using polyester sheets as a storage Device; Collection of oocyte–granulosa cell complexes by enzymatic treatment; and in vitro growth (IVG), in vitro maturation (IVM), and IVF, with embryo transplantation to pseudopregnant mice. Main Outcome Measure(s) Histological analysis of vitrified and warmed ovaries from several animal species; measurement of successful rates in IVG, IVM, and IVF of mouse oocytes in oocyte–granulosa cell complexes collected from the vitrified and warmed ovaries; and achievement of pup birth. Result(s) The vitrification method used was effective for storage of various animal ovaries. The oocytes enclosed in preantral follicles that were yielded from the vitrified–warmed ovaries preserved the capacity for developing into pups after IVG, IVM, and IVF. Conclusion(s) The findings indicated that ovarian cryopreservation by vitrification and subsequent IVG, IVM, and IVF are promising methods for restoring young cancer patients' fertility. The practical information presented here is applicable to human ovarian tissues.

  • In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries.
    Fertility and sterility, 2004
    Co-Authors: Akiko Hasegawa, Yukari Hamada, Tzvetozar Mehandjiev, Koji Koyama
    Abstract:

    To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries. Experimental animal study. University-based research laboratory. Normal (C57BL/6xDBA2) F1 mice in a laboratory environment. Vitrification of mouse ovaries using polyester sheets as a storage Device; Collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF). We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries. The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries. The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos.

  • In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries.
    Fertility and Sterility, 2004
    Co-Authors: Akiko Hasegawa, Yukari Hamada, Tzvetozar Mehandjiev, Koji Koyama
    Abstract:

    Abstract Objective To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries. Design Experimental animal study. Setting University-based research laboratory. Animal(s) Normal (C57BL/6xDBA2) F1 mice in a laboratory environment. Intervention(s) Vitrification of mouse ovaries using polyester sheets as a storage Device; Collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF). Main outcome measure(s) We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries. Result(s) The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries. Conclusion(s) The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos.

Akiko Hasegawa - One of the best experts on this subject based on the ideXlab platform.

  • Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in vitro fertilization.
    Fertility and sterility, 2006
    Co-Authors: Akiko Hasegawa, Nahoko Mochida, Toshitada Ogasawara, Koji Koyama
    Abstract:

    To determine an optimum condition for vitrification of various animal species and to examine the developing capacity into pups of mouse oocytes in preantral follicles originating from cryopreserved ovaries. Experimental animal study. Laboratory environment. Normal (C57BL/6 x DBA2) F1 mice. Vitrification of animal ovaries using polyester sheets as a storage Device; Collection of oocyte-granulosa cell complexes by enzymatic treatment; and in vitro growth (IVG), in vitro maturation (IVM), and IVF, with embryo transplantation to pseudopregnant mice. Histological analysis of vitrified and warmed ovaries from several animal species; measurement of successful rates in IVG, IVM, and IVF of mouse oocytes in oocyte-granulosa cell complexes collected from the vitrified and warmed ovaries; and achievement of pup birth. The vitrification method used was effective for storage of various animal ovaries. The oocytes enclosed in preantral follicles that were yielded from the vitrified-warmed ovaries preserved the capacity for developing into pups after IVG, IVM, and IVF. The findings indicated that ovarian cryopreservation by vitrification and subsequent IVG, IVM, and IVF are promising methods for restoring young cancer patients' fertility. The practical information presented here is applicable to human ovarian tissues.

  • Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in vitro fertilization.
    Fertility and Sterility, 2006
    Co-Authors: Akiko Hasegawa, Nahoko Mochida, Toshitada Ogasawara, Koji Koyama
    Abstract:

    Objective To determine an optimum condition for vitrification of various animal species and to examine the developing capacity into pups of mouse oocytes in preantral follicles originating from cryopreserved ovaries. Design Experimental animal study. Setting Laboratory environment. Animal(s) Normal (C57BL/6 × DBA2) F1 mice. Intervention(s) Vitrification of animal ovaries using polyester sheets as a storage Device; Collection of oocyte–granulosa cell complexes by enzymatic treatment; and in vitro growth (IVG), in vitro maturation (IVM), and IVF, with embryo transplantation to pseudopregnant mice. Main Outcome Measure(s) Histological analysis of vitrified and warmed ovaries from several animal species; measurement of successful rates in IVG, IVM, and IVF of mouse oocytes in oocyte–granulosa cell complexes collected from the vitrified and warmed ovaries; and achievement of pup birth. Result(s) The vitrification method used was effective for storage of various animal ovaries. The oocytes enclosed in preantral follicles that were yielded from the vitrified–warmed ovaries preserved the capacity for developing into pups after IVG, IVM, and IVF. Conclusion(s) The findings indicated that ovarian cryopreservation by vitrification and subsequent IVG, IVM, and IVF are promising methods for restoring young cancer patients' fertility. The practical information presented here is applicable to human ovarian tissues.

  • In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries.
    Fertility and sterility, 2004
    Co-Authors: Akiko Hasegawa, Yukari Hamada, Tzvetozar Mehandjiev, Koji Koyama
    Abstract:

    To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries. Experimental animal study. University-based research laboratory. Normal (C57BL/6xDBA2) F1 mice in a laboratory environment. Vitrification of mouse ovaries using polyester sheets as a storage Device; Collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF). We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries. The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries. The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos.

  • In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries.
    Fertility and Sterility, 2004
    Co-Authors: Akiko Hasegawa, Yukari Hamada, Tzvetozar Mehandjiev, Koji Koyama
    Abstract:

    Abstract Objective To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries. Design Experimental animal study. Setting University-based research laboratory. Animal(s) Normal (C57BL/6xDBA2) F1 mice in a laboratory environment. Intervention(s) Vitrification of mouse ovaries using polyester sheets as a storage Device; Collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF). Main outcome measure(s) We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries. Result(s) The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries. Conclusion(s) The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos.

Alessandro Puzziello - One of the best experts on this subject based on the ideXlab platform.

  • Post-thyroidectomy complications. The role of the Device: bipolar vs ultrasonic Device: Collection of data from 1,846 consecutive patients undergoing thyroidectomy
    American Journal of Surgery, 2015
    Co-Authors: Maurizio De Palma, Ludovico Rosato, Fabiana Zingone, G. Orlando, Antonio Antonino, Mario Vitale, Alessandro Puzziello
    Abstract:

    Abstract Background Specific complications after thyroid surgery, such as recurrent laryngeal nerve injury (RLN) or hypoparathyroidism, are feared because they may give rise to a lifelong disability for the patient. The aim of this study was to evaluate the possible association between the types of Device used (bipolar vs ultrasound-based harmonic scalpel defined Harmonic Focus) and major postoperative complications. Methods During a 1-year period, between October 2010 and October 2011, Italian Endocrine Surgery Units affiliated with the Italian Endocrine Surgery Units Association collected data on all consecutive patients older than 18 years who had undergone primary total thyroidectomy, near total thyroidectomy, and completion thyroidectomy. The data were included in a dataset, listing demographic variables, details on the surgical procedure, and 2 major complications of the thyroid surgery: postoperative RLN palsy/hypomobility and hypocalcemia. Results Our population comprised 1,846 subjects (78.6% women, median age 52 years). Six hundred four (32.7%) subjects underwent thyroidectomy by bipolar forceps and 1,242 (67.3%) by ultrasonic Device. The risk of hypocalcemia in subjects undergoing thyroidectomy by ultrasonic Device was similar to those undergoing thyroidectomy by bipolar after adjusting for sex, type of thyroidectomy, and central lymphadenectomy (odds ratio .94, 95% confidence interval .76 to 1.17). Subjects who underwent thyroidectomy by ultrasonic Device had a lower risk of RLN paralysis compared with those undergoing thyroidectomy by bipolar forceps also after adjusting for central lymphadenectomy (odds ratio .39, 95% confidence interval .2 to .7). Conclusion This multicenter study acknowledges the value of the ultrasonic Device as a protective factor only for RLN palsy, confirming nodal dissection as a risk factor for postoperative hypocalcemia and vocal folds disorders.

Tzvetozar Mehandjiev - One of the best experts on this subject based on the ideXlab platform.

  • In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries.
    Fertility and sterility, 2004
    Co-Authors: Akiko Hasegawa, Yukari Hamada, Tzvetozar Mehandjiev, Koji Koyama
    Abstract:

    To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries. Experimental animal study. University-based research laboratory. Normal (C57BL/6xDBA2) F1 mice in a laboratory environment. Vitrification of mouse ovaries using polyester sheets as a storage Device; Collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF). We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries. The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries. The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos.

  • In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries.
    Fertility and Sterility, 2004
    Co-Authors: Akiko Hasegawa, Yukari Hamada, Tzvetozar Mehandjiev, Koji Koyama
    Abstract:

    Abstract Objective To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries. Design Experimental animal study. Setting University-based research laboratory. Animal(s) Normal (C57BL/6xDBA2) F1 mice in a laboratory environment. Intervention(s) Vitrification of mouse ovaries using polyester sheets as a storage Device; Collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF). Main outcome measure(s) We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries. Result(s) The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries. Conclusion(s) The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos.

Yukari Hamada - One of the best experts on this subject based on the ideXlab platform.

  • In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries.
    Fertility and sterility, 2004
    Co-Authors: Akiko Hasegawa, Yukari Hamada, Tzvetozar Mehandjiev, Koji Koyama
    Abstract:

    To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries. Experimental animal study. University-based research laboratory. Normal (C57BL/6xDBA2) F1 mice in a laboratory environment. Vitrification of mouse ovaries using polyester sheets as a storage Device; Collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF). We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries. The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries. The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos.

  • In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries.
    Fertility and Sterility, 2004
    Co-Authors: Akiko Hasegawa, Yukari Hamada, Tzvetozar Mehandjiev, Koji Koyama
    Abstract:

    Abstract Objective To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries. Design Experimental animal study. Setting University-based research laboratory. Animal(s) Normal (C57BL/6xDBA2) F1 mice in a laboratory environment. Intervention(s) Vitrification of mouse ovaries using polyester sheets as a storage Device; Collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF). Main outcome measure(s) We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries. Result(s) The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries. Conclusion(s) The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos.