The Experts below are selected from a list of 201 Experts worldwide ranked by ideXlab platform
Pooria Gill - One of the best experts on this subject based on the ideXlab platform.
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Circular Dichroism techniques: Biomolecular and nanostructural analyses- A review
Chemical Biology and Drug Design, 2009Co-Authors: Bijan Ranjbar, Pooria GillAbstract:This paper reviews the best known techniques using circular Dichroism spectroscopy such as conventional circular Dichroism (i.e. electronic circular Dichroism), magnetic circular Dichroisms (magnetic vibrational circular Dichroism, x-ray magnetic circular Dichroism), fluorescence detected circular Dichroism, near-infrared circular Dichroism, vibrational circular Dichroism, Fourier transform infrared circular Dichroism, high pressure liquid chromatography circular Dichroism, stopped-flow circular Dichroism, and synchrotron radiation circular Dichroism. Also, we have described here the most important applications of circular Dichroism spectroscopy in structural biochemistry and nanoscience.
Robert W. Woody - One of the best experts on this subject based on the ideXlab platform.
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4 circular Dichroism
Methods in Enzymology, 1995Co-Authors: Robert W. WoodyAbstract:Publisher Summary Circular Dichroism (CD) is a spectroscopic method which depends on the fact that certain molecules interact differently with right and left circularly polarized light. Circularly polarized light is chiral—that is, it occurs in two nonsuperimposable forms that are mirror images of one another. To discriminate between the two chiral forms of light, a molecule must be chiral, including the vast majority of biological molecules. A method that can discern the subtle differences between non superimposable mirror image molecules (enantiomers) must be highly sensitive to the three-dimensional features of molecules—that is, to conformation. Binding of ligands or protein-protein and protein–DNA interactions can also alter the circular Dichroism spectrum of the protein and/or nucleic acid. These changes in CD can be used to determine equilibrium constants, and they can also provide evidence for conformational changes. Thus, CD can provide information about the secondary structure of proteins and nucleic acids and about the binding of ligands to these types of macromolecule.
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circular Dichroism principles and applications
1994Co-Authors: Nina Berova, 香爾 中西, Robert W. WoodyAbstract:PARTIAL TABLE OF CONTENTS: Circular Dichroism: An Introduction (G. Snatzke). From Pasteur to Parity Nonconservation: Theories of the Origin of Molecular Chirality (S. Mason). Theoretical Approach to Electronic Optical Activity (A. Koslowski, et al.). Vibrational Optical Acivity Theory (L. Nafie & T. Freedman). Fast Time-Resolved Circular Dichroism Measurements (D. Kliger & J. Lewis). The Octant Rule (D. Lightner). Circular Dichroism and Chirality of Dienes (J. Gawroski). Exciton Chirality Method: Principles and Applications (N. Berova & K. Nakanishi). A Model for How Polymers Amplify Chirality (M. Green). Induced CD or Polymers (E. Yashima & Y. Okamoto). Application of CD to the Study of Some Cholesteric Mesophase (G. Gottarelli & G. Spada). Circular Dichroism of Inorganic Complexes: Interpretation and Applications (R. Kuroda). Circular Dichroism of Peptides and Proteins (N. Sreerama & R. Woody). Peptide and Protein Conformational Studies with Vibrational Circular Dichroism and Related Spectroscopies (T. Keiderling). HPLC-CD: Stereochemical Analysis at Work (P. Salvadori, et al.). Applications of Chiroptical Spectroscopy in the Characterization of Compounds Having Pharmaceutical Importance (H. Brittain). Index.
Bijan Ranjbar - One of the best experts on this subject based on the ideXlab platform.
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Circular Dichroism techniques: Biomolecular and nanostructural analyses- A review
Chemical Biology and Drug Design, 2009Co-Authors: Bijan Ranjbar, Pooria GillAbstract:This paper reviews the best known techniques using circular Dichroism spectroscopy such as conventional circular Dichroism (i.e. electronic circular Dichroism), magnetic circular Dichroisms (magnetic vibrational circular Dichroism, x-ray magnetic circular Dichroism), fluorescence detected circular Dichroism, near-infrared circular Dichroism, vibrational circular Dichroism, Fourier transform infrared circular Dichroism, high pressure liquid chromatography circular Dichroism, stopped-flow circular Dichroism, and synchrotron radiation circular Dichroism. Also, we have described here the most important applications of circular Dichroism spectroscopy in structural biochemistry and nanoscience.
Valerie Gabelica - One of the best experts on this subject based on the ideXlab platform.
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mass resolved electronic circular Dichroism ion spectroscopy
Science, 2020Co-Authors: Steven Daly, Frederic Rosu, Valerie GabelicaAbstract:DNA and proteins are chiral: Their three-dimensional structures cannot be superimposed with their mirror images. Circular Dichroism spectroscopy is widely used to characterize chiral compounds, but data interpretation is difficult in the case of mixtures. We recorded the electronic circular Dichroism spectra of DNA helices separated in a mass spectrometer. We studied guanine-rich strands having various secondary structures, electrosprayed them as negative ions, irradiated them with an ultraviolet nanosecond optical parametric oscillator laser, and measured the difference in electron photodetachment efficiency between left and right circularly polarized light. The reconstructed circular Dichroism ion spectra resembled those of their solution-phase counterparts, thereby allowing us to assign the DNA helical topology. The ability to measure circular Dichroism directly on biomolecular ions expands the capabilities of mass spectrometry for structural analysis.
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Mass-resolved electronic circular Dichroism ion spectroscopy
Science, 2020Co-Authors: Steven Daly, Frederic Rosu, Valerie GabelicaAbstract:DNA and proteins are chiral: their three-dimensional structure cannot be superimposed with its mirror image. Circular Dichroism spectroscopy is widely used to characterize chiral compounds, but data interpretation is difficult in the case of mixtures. We recorded the electronic circular Dichroism spectra of DNA helices separated in a mass spectrometer. We studied guanine-rich strands having various secondary structures, electrosprayed them as negative ions, irradiated them with a UV-vis nanosecond OPO laser, and measured the difference in electron photodetachment efficiency between left and right circularly polarized light. The reconstructed circular Dichroism ion spectra resemble their solution counterparts, thereby allowing us to assign the DNA helical topology. The ability to measure circular Dichroism directly on biomolecular ions expands the capabilities of mass spectrometry for structural analysis.
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mass resolved electronic circular Dichroism ion spectroscopy
arXiv: Biomolecules, 2020Co-Authors: Steven Daly, Frederic Rosu, Valerie GabelicaAbstract:DNA and proteins are chiral: their three-dimensional structure cannot be superimposed with its mirror image. Circular Dichroism spectroscopy is widely used to characterize chiral compounds, but data interpretation is difficult in the case of mixtures. We recorded for the first time the electronic circular Dichroism spectra of DNA helices separated in a mass spectrometer. We electrosprayed guanine-rich strands having various secondary structures as negative ions, irradiated them with a laser, and measured the difference in electron photodetachment efficiency between left and right circularly polarized light. The reconstructed circular Dichroism ion spectra resemble the solution ones, thereby allowing us to assign the DNA helical topology. The ability to measure circular Dichroism directly on biomolecular ions expands the capabilities of mass spectrometry for structural analysis.
Mikel B Holcomb - One of the best experts on this subject based on the ideXlab platform.
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x ray linear Dichroism dependence on ferroelectric polarization
Journal of Physics: Condensed Matter, 2012Co-Authors: Srinivas Polisetty, Jinling Zhou, J Karthik, Anoop R Damodaran, Disheng Chen, A Scholl, Lane W Martin, Mikel B HolcombAbstract:X-ray absorption spectroscopy and photoemission electron microscopy are techniques commonly used to determine the magnetic properties of thin films, crystals, and heterostructures. Recently, these methods have been used in the study of magnetoelectrics and multiferroics. The analysis of such materials has been compromised by the presence of multiple order parameters and the lack of information on how to separate these coupled properties. In this work, we shed light on the manifestation of Dichroism from ferroelectric polarization and atomic structure using photoemission electron microscopy and x-ray absorption spectroscopy. Linear Dichroism arising from the ferroelectric order in the PbZr0:2Ti0:8O3 thin films was studied as a function of incident x-ray polarization and geometry to unambiguously determine the angular dependence of the ferroelectric contribution to the Dichroism. These measurements allow us to examine the contribution of surface charges and ferroelectric polarization as potential mechanisms for linear Dichroism. The x-ray linear Dichroism from ferroelectric order revealed an angular dependence based on the angle between the ferroelectric polarization direction and the x-ray polarization axis, allowing a formula for linear Dichroism in ferroelectric samples to be defined.
