The Experts below are selected from a list of 156 Experts worldwide ranked by ideXlab platform
Ho-chul Shin - One of the best experts on this subject based on the ideXlab platform.
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a simple extraction method for the simultaneous detection of tetramisole and Diethylcarbamazine in milk eggs and porcine muscle using gradient liquid chromatography tandem mass spectrometry
Food Chemistry, 2016Co-Authors: Dan Zhang, Seong Kwan Kim, Soo Jean Shin, Dong Soon Kim, Jin Suk Kim, Jin-a Park, Jae-han Shim, Sung-chul Shin, A Abd M Elaty, Ho-chul ShinAbstract:Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and Diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, Diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 °C for 1h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R(2) ⩾ 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were <20%. The limit of quantification was 0.2 and 2 ng/g for tetramisole and Diethylcarbamazine, respectively. An analytical survey of samples purchased from large markets showed that none of the samples contained any of the target analytes. To the best of our knowledge, this is the first report on the quantitative determination of tetramisole and Diethylcarbamazine in animal food products.
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A simple extraction method for the simultaneous detection of tetramisole and Diethylcarbamazine in milk, eggs, and porcine muscle using gradient liquid chromatography-tandem mass spectrometry
Food Chemistry, 2016Co-Authors: Dan Zhang, Seong Kwan Kim, Soo Jean Shin, Dong Soon Kim, A. M.; Abd El-aty, Jin Suk Kim, Jin-a Park, Jae-han Shim, Sung-chul Shin, Ho-chul ShinAbstract:Abstract Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and Diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, Diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 ??C for 1 h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R2 ??? 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were
Dan Zhang - One of the best experts on this subject based on the ideXlab platform.
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a simple extraction method for the simultaneous detection of tetramisole and Diethylcarbamazine in milk eggs and porcine muscle using gradient liquid chromatography tandem mass spectrometry
Food Chemistry, 2016Co-Authors: Dan Zhang, Seong Kwan Kim, Soo Jean Shin, Dong Soon Kim, Jin Suk Kim, Jin-a Park, Jae-han Shim, Sung-chul Shin, A Abd M Elaty, Ho-chul ShinAbstract:Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and Diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, Diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 °C for 1h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R(2) ⩾ 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were <20%. The limit of quantification was 0.2 and 2 ng/g for tetramisole and Diethylcarbamazine, respectively. An analytical survey of samples purchased from large markets showed that none of the samples contained any of the target analytes. To the best of our knowledge, this is the first report on the quantitative determination of tetramisole and Diethylcarbamazine in animal food products.
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A simple extraction method for the simultaneous detection of tetramisole and Diethylcarbamazine in milk, eggs, and porcine muscle using gradient liquid chromatography-tandem mass spectrometry
Food Chemistry, 2016Co-Authors: Dan Zhang, Seong Kwan Kim, Soo Jean Shin, Dong Soon Kim, A. M.; Abd El-aty, Jin Suk Kim, Jin-a Park, Jae-han Shim, Sung-chul Shin, Ho-chul ShinAbstract:Abstract Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and Diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, Diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 ??C for 1 h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R2 ??? 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were
Richard J Martin - One of the best experts on this subject based on the ideXlab platform.
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Diethylcarbamazine activates trp channels including trp 2 in filaria brugia malayi
Communications Biology, 2020Co-Authors: Saurabh Verma, Sudhanva S Kashyap, Alan P Robertson, Richard J MartinAbstract:Diethylcarbamazine is an important classic drug used for prevention and treatment of lymphatic filariasis and loiasis, diseases caused by filarial nematodes. Despite many studies, its site of action has not been established. Until now, the consensus has been that Diethylcarbamazine works by activating host immune systems, not by a direct action on the parasites. Here we show that low concentrations of Diethylcarbamazine have direct and rapid (<30 s) temporary spastic paralyzing effects on the parasites that lasts around 4 h, which is produced by Diethylcarbamazine opening TRP channels in muscle of Brugia malayi involving TRP-2 (TRPC-like channel subunits). GON-2 and CED-11, TRPM-like channel subunits, also contributed to Diethylcarbamazine responses. Opening of these TRP channels produces contraction and subsequent activation of calcium-dependent SLO-1K channels. Recovery from the temporary paralysis is consistent with inactivation of TRP channels. Our observations elucidate mechanisms for the rapid onset and short-lasting therapeutic actions of Diethylcarbamazine. Verma et al. describe a new mechanism for Diethylcarbamazine action, a drug classically used to treat lymphatic filariasis and loiasis. They propose that it induces rapid temporary spastic paralysis of parasites by opening TRP channels, including TRP-2 in muscle of Brugia malayi. Inactivation of TRP channels explain its short-lasting therapeutic activity.
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Diethylcarbamazine activates TRP channels including TRP-2 in filaria, Brugia malayi.
Communications biology, 2020Co-Authors: Saurabh Verma, Sudhanva S Kashyap, Alan P Robertson, Richard J MartinAbstract:Diethylcarbamazine is an important classic drug used for prevention and treatment of lymphatic filariasis and loiasis, diseases caused by filarial nematodes. Despite many studies, its site of action has not been established. Until now, the consensus has been that Diethylcarbamazine works by activating host immune systems, not by a direct action on the parasites. Here we show that low concentrations of Diethylcarbamazine have direct and rapid (
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Diethylcarbamazine increases activation of voltage-activated potassium (SLO-1) currents in Ascaris suum and potentiates effects of emodepside.
PLoS neglected tropical diseases, 2014Co-Authors: Samuel K. Buxton, Alan P Robertson, Richard J MartinAbstract:Diethylcarbamazine is a drug that is used for the treatment of filariasis in humans and animals; it also has effects on intestinal nematodes, but its mechanism of action remains unclear. Emodepside is a resistance-busting anthelmintic approved for treating intestinal parasitic nematodes in animals. The novel mode of action and resistance-breaking properties of emodepside has led to its use against intestinal nematodes of animals, and as a candidate drug for treating filarial parasites. We have previously demonstrated effects of emodepside on SLO-1 K+-like currents in Ascaris suum. Here, we demonstrate that Diethylcarbamazine, which has been proposed to work through host mediated effects, has direct effects on a nematode parasite, Ascaris suum. It increases activation of SLO-1 K+ currents and potentiates effects of emodepside. Our results suggest consideration of the combination of emodepside and Diethylcarbamazine for therapy, which is predicted to be synergistic. The mode of action of Diethylcarbamazine may involve effects on parasite signaling pathways (including nitric oxide) as well as effects mediated by host inflammatory mediators.
Seong Kwan Kim - One of the best experts on this subject based on the ideXlab platform.
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a simple extraction method for the simultaneous detection of tetramisole and Diethylcarbamazine in milk eggs and porcine muscle using gradient liquid chromatography tandem mass spectrometry
Food Chemistry, 2016Co-Authors: Dan Zhang, Seong Kwan Kim, Soo Jean Shin, Dong Soon Kim, Jin Suk Kim, Jin-a Park, Jae-han Shim, Sung-chul Shin, A Abd M Elaty, Ho-chul ShinAbstract:Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and Diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, Diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 °C for 1h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R(2) ⩾ 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were <20%. The limit of quantification was 0.2 and 2 ng/g for tetramisole and Diethylcarbamazine, respectively. An analytical survey of samples purchased from large markets showed that none of the samples contained any of the target analytes. To the best of our knowledge, this is the first report on the quantitative determination of tetramisole and Diethylcarbamazine in animal food products.
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A simple extraction method for the simultaneous detection of tetramisole and Diethylcarbamazine in milk, eggs, and porcine muscle using gradient liquid chromatography-tandem mass spectrometry
Food Chemistry, 2016Co-Authors: Dan Zhang, Seong Kwan Kim, Soo Jean Shin, Dong Soon Kim, A. M.; Abd El-aty, Jin Suk Kim, Jin-a Park, Jae-han Shim, Sung-chul Shin, Ho-chul ShinAbstract:Abstract Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and Diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, Diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 ??C for 1 h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R2 ??? 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were
Dong Soon Kim - One of the best experts on this subject based on the ideXlab platform.
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a simple extraction method for the simultaneous detection of tetramisole and Diethylcarbamazine in milk eggs and porcine muscle using gradient liquid chromatography tandem mass spectrometry
Food Chemistry, 2016Co-Authors: Dan Zhang, Seong Kwan Kim, Soo Jean Shin, Dong Soon Kim, Jin Suk Kim, Jin-a Park, Jae-han Shim, Sung-chul Shin, A Abd M Elaty, Ho-chul ShinAbstract:Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and Diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, Diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 °C for 1h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R(2) ⩾ 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were <20%. The limit of quantification was 0.2 and 2 ng/g for tetramisole and Diethylcarbamazine, respectively. An analytical survey of samples purchased from large markets showed that none of the samples contained any of the target analytes. To the best of our knowledge, this is the first report on the quantitative determination of tetramisole and Diethylcarbamazine in animal food products.
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A simple extraction method for the simultaneous detection of tetramisole and Diethylcarbamazine in milk, eggs, and porcine muscle using gradient liquid chromatography-tandem mass spectrometry
Food Chemistry, 2016Co-Authors: Dan Zhang, Seong Kwan Kim, Soo Jean Shin, Dong Soon Kim, A. M.; Abd El-aty, Jin Suk Kim, Jin-a Park, Jae-han Shim, Sung-chul Shin, Ho-chul ShinAbstract:Abstract Analysis of residual quantities of contaminants in foods of animal origin is crucial for quality control of consumer products. This study was aimed to develop a simple and raid analytical method for detection of tetramisole and Diethylcarbamazine using gradient liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Tetramisole, Diethylcarbamazine, and guaifenesin (as an internal standard) were extracted from milk, eggs, and porcine muscle using acetonitrile followed by partitioning at -20 ??C for 1 h. No extract purification was deemed necessary. The analytes were separated on C18 column using ammonium formate both in water and methanol. Good linearity was achieved over the tested concentration range with R2 ??? 0.974. Recovery at two fortification levels ranged between 67.47% and 97.38%. The intra- and inter-day precisions were