The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ron Orlando - One of the best experts on this subject based on the ideXlab platform.
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on target endoglycosidase Digestion matrix assisted laser desorption ionization mass spectrometry of glycopeptides
Rapid Communications in Mass Spectrometry, 2001Co-Authors: Jennifer Colangelo, Ron OrlandoAbstract:The Digestion of glycopeptides with endoglycosidases can be used in the process of their structural characterization, and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) is often used to analyze the products of these Digestions. In the currently accepted protocol for the endoglycosidase Digestion of glycopeptides on the MALDI target, the target must be incubated at 37 °C, and an hour or more is needed for Digestion. We have modified the procedure so that the process can be performed at room temperature in 5 to 15 min, and Digestions are performed in the presence of a MALDI matrix. The endoglycosidases used for Digestion were endoglycosidase H and peptide-N-glycosidase F. Glycopeptides from asialofetuin and endopolygalacturonase (EPG) II were used as standards because their glycan structures have been previously characterized. Glycopeptides with unknown glycan structures were also digested, including glycopeptides from pectate lyase, EPG I, and pectin methylesterase from Aspergillus niger. Copyright © 2001 John Wiley & Sons, Ltd.
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on target exoglycosidase Digestions maldi ms for determining the primary structures of carbohydrate chains
Analytical Chemistry, 1999Co-Authors: Jennifer Colangelo, Ron OrlandoAbstract:One method used to determine the primary sequence of oligosaccharides is to digest them with exoglycosidases and analyze the resulting Digestion products by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Previous research has demonstrated that these Digestions can be performed on the MALDI target. However, the procedure requires the sample to be incubated at elevated temperatures, and complete Digestion requires a few hours. We demonstrate new conditions that permit exoglycosidase Digestions to be performed on the MALDI target at room temperature within 30 min. Oligosaccharide standards were digested with one or more exoglycosidases to show that the enzymes retain their activity and specificity under these new reaction conditions. Using this method, the primary sequences of carbohydrate chains can be determined in a relatively short amount of time.
Bruce C Anderson - One of the best experts on this subject based on the ideXlab platform.
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enhanced biogas production from anaerobic co Digestion of municipal wastewater treatment sludge and fat oil and grease fog by a modified two stage thermophilic digester system with selected thermo chemical pre treatment
Renewable Energy, 2015Co-Authors: Chenxi Li, Pascale Champagne, Bruce C AndersonAbstract:Anaerobic co-Digestions with fat, oil and grease (FOG) were investigated in two-stage thermophilic (55 °C) semi-continuous flow co-Digestion systems. One two-stage co-Digestion system (System I) was modified to incorporate a thermo-chemical pre-treatment of pH = 10 at 55 °C, which was the best pre-treatment condition for FOG co-Digestion identified during laboratory-scale biochemical methane potential (BMP) testing. The other two-stage co-Digestion system (System II) was operated without a pre-treatment process. The anaerobic digester of each Digestion system had a hydraulic retention time (HRT) of 24 days. An organic loading rate (OLR) of 1.83 ± 0.09 g TVS/L·d was applied to each Digestion system. It was found that System I effectively enhanced biogas production as the thermo-chemical pre-treatment improved the substrate hydrolysis including increased COD solubilization and VFA concentrations. Overall, the modified System I yielded a 25.14 ± 2.14 L/d biogas production rate, which was substantially higher than the 18.73 ± 1.11 L/d obtained in the System II.
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biogas production performance of mesophilic and thermophilic anaerobic co Digestion with fat oil and grease in semi continuous flow digesters effects of temperature hydraulic retention time and organic loading rate
Environmental Technology, 2013Co-Authors: Chenxi Li, Pascale Champagne, Bruce C AndersonAbstract:Anaerobic co-Digestions with fat, oil, and grease (FOG) were investigated in semi-continuous flow digesters under various operating conditions. The effects of hydraulic retention times (HRTs) of 12 and 24 days, organic loading rates (OLRs) between 1.19 and 8.97 g TVS/Ld, and Digestion temperatures of 37°C and 55°C on biogas production were evaluated. It was proposed that, compared to anaerobic Digestion with wastewater treatment plant sludge (primary raw sludge), semi-continuous flow anaerobic co-Digestion with FOG could effectively enhance biogas and methane production. Thermophilic (55°C) co-Digestions exhibited higher biogas production and degradation of organics than mesophilic co-Digestions. The best biogas production rate of 17.4±0.86 L/d and methane content 67.9±1.46% was obtained with a thermophilic co-Digestion at HRT=24 days and OLR=2.43±0.15 g TVS/Ld. These were 32.8% and 7.10% higher than the respective values from the mesophilic co-Digestion under similar operating conditions.
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effects of ultrasonic and thermo chemical pre treatments on methane production from fat oil and grease fog and synthetic kitchen waste kw in anaerobic co Digestion
Bioresource Technology, 2013Co-Authors: Chenxi Li, Pascale Champagne, Bruce C AndersonAbstract:Abstract The effects of ultrasonic and thermo-chemical pre-treatments on the methane production potential of anaerobic co-Digestion with synthetic kitchen waste (KW) or fat, oil and grease (FOG) were investigated. Non-linear regressions were fitted to accurately assess and compare the methane production from co-Digestion under the various pre-treatment conditions and to achieve representative simulations and predictions. Ultrasonic pre-treatment was not found to improve methane production effectively from either FOG co-Digestion or KW co-Digestions. Thermo-chemical pre-treatment could increase methane production yields from both FOG and KW co-Digestions. COD solubilization was found to effectively represent the effects of pre-treatment. A comprehensive evaluation indicated that the thermo-chemical pre-treatments of pH = 10, 55 °C and pH = 8, 55 °C provided the best conditions to increase methane production from FOG and KW co-Digestions, respectively. The most effective enhancement of biogas production (288 ± 0.85 mL CH4/g TVS) was achieved from thermo-chemically pre-treated FOG co-Digestion, which was 9.9 ± 1.5% higher than FOG co-Digestion without thermo-chemical pre-treatment.
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evaluating and modeling biogas production from municipal fat oil and grease and synthetic kitchen waste in anaerobic co Digestions
Bioresource Technology, 2011Co-Authors: Chenxi Li, Pascale Champagne, Bruce C AndersonAbstract:The feasibility of using synthetic kitchen waste (KW) and fat, oil, and grease (FOG) as co-substrates in the anaerobic Digestion of waste activated sludge (WAS) was investigated using two series of biochemical methane potential (BMP) tests. Ranges of ideal substrate to inoculum (S/I) ratio were determined for the FOG (0.25–0.75) and KW (0.80–1.26) as single substrates in the first experiment. The second experiment, which estimated the methane production performances of FOG and KW as co-substrates for WAS co-Digestion, was conducted based on the optimal parameters selected from the results of the first experiment. Results indicated that co-Digestions with FOG and KW enhanced methane production from 117 ± 2.02 mL/gTVS (with only WAS) to 418 ± 13.7 mL/gTVS and 324 ± 4.11 mL/gTVS, respectively. FOG exhibited more biogas production than KW as co-substrate. Non-linear regression results showed that co-substrate addition shortened the lag phases of organic biodegradation from 81.8 (with only WAS) to 28.3 h with FOG and 3.90 h with KW.
David J. Harvey - One of the best experts on this subject based on the ideXlab platform.
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Rapid Approach for Sequencing Neutral Oligosaccharides by Exoglycosidase Digestion and MGrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
2016Co-Authors: Bernhard Kiister, Thomas J. P. Naven, David J. HarveyAbstract:A new way of combining exoglycosidase Digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS) is described which permits the structural characterization of underivatized oligosaccharides on low picomole amounts of starting material. The key feature of the new approach is that an oligosaccharide sample can be recovered after a MALDI experiment and a series of sequential exoglycosidase Digestions can be carried out on that sample within a single working day. The following steps are involved: (i) recording a molecular mass profile of the starting material by MALDI/TOF-MS using a mixture of 2, s dihydroxybenzoic acid and 1-hydroxyisoquinoline as the matrix; (ii) recovery of the sample from the target and removal of the matrix by droplet dialysis (molecular mass cut-off 500 Da); (iii) exoglycosidase Digestion in a volume of 1 PI; (iv) removal of the incubation buffer by droplet dialysis; (v) removal of the enzyme by absorption on a Nafion membrane and (vi) start of the next cycle from (i). The method exhibits the following advantages over traditional oligosaccharide sequencing techniques: (i) analysis of Digestion products by MALDI/TOF-MS is much faster than by chromatographic techniques; (ii) no derivatization of the analyte is required; (iii) exoglycosidase Digestions work faster in small reaction volumes because substrate concentrations are closer to the K, of the enzyme; (iv) advanced sample handling techniques ensure reduced losses and (v) no sample splitting is needed for analysis and therefore the sensitivity of the overall method is increased. The method is illustrated by the analysis of isolated glycans and complex mixtures derived from chicken ovalbumin and human immunoglobulin G. KEY WORDS: oligosaccharides; exoglycosidases; matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; ovalbumin; immunoglobulin
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rapid approach for sequencing neutral oligosaccharides by exoglycosidase Digestion and matrix assisted laser desorption ionization time of flight mass spectrometry
Journal of Mass Spectrometry, 1996Co-Authors: Bernhard Kuster, Thomas J. P. Naven, David J. HarveyAbstract:A new way of combining exoglycosidase Digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS) is described which permits the structural characterization of underivatized oligosaccharides on low picomole amounts of starting material. The key feature of the new approach is that an oligosaccharide sample can be recovered after a MALDI experiment and a series of sequential exoglycosidase Digestions can be carried out on that sample within a single working day. The following steps are involved : (i) recording a molecular mass profile of the starting material by MALDI/TOF-MS using a mixture of 2,5-dihydroxybenzoic acid and 1-hydroxyisoquinoline as the matrix ; (ii) recovery of the sample from the target and removal of the matrix by droplet dialysis (molecular mass cut-off 500 Da) ; (iii) exoglycosidase Digestion in a volume of 1 μl ; (iv) removal of the incubation buffer by droplet dialysis ; (v) removal of the enzyme by absorption on a Nafion membrane and (vi) start of the next cycle from (i). The method exhibits the following advantages over traditional oligosaccharide sequencing techniques : (i) analysis of Digestion products by MALDI/TOF-MS is much faster than by chromatographic techniques ; (ii) no derivatization of the analyte is required ; (iii) exoglycosidase Digestions work faster in small reaction volumes because substrate concentrations are closer to the K m of the enzyme ; (iv) advanced sample handling techniques ensure reduced losses and (v) no sample splitting is needed for analysis and therefore the sensitivity of the overall method is increased. The method is illustrated by the analysis of isolated glycans and complex mixtures derived from chicken ovalbumin and human immunoglobulin G.
Jennifer Colangelo - One of the best experts on this subject based on the ideXlab platform.
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on target endoglycosidase Digestion matrix assisted laser desorption ionization mass spectrometry of glycopeptides
Rapid Communications in Mass Spectrometry, 2001Co-Authors: Jennifer Colangelo, Ron OrlandoAbstract:The Digestion of glycopeptides with endoglycosidases can be used in the process of their structural characterization, and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) is often used to analyze the products of these Digestions. In the currently accepted protocol for the endoglycosidase Digestion of glycopeptides on the MALDI target, the target must be incubated at 37 °C, and an hour or more is needed for Digestion. We have modified the procedure so that the process can be performed at room temperature in 5 to 15 min, and Digestions are performed in the presence of a MALDI matrix. The endoglycosidases used for Digestion were endoglycosidase H and peptide-N-glycosidase F. Glycopeptides from asialofetuin and endopolygalacturonase (EPG) II were used as standards because their glycan structures have been previously characterized. Glycopeptides with unknown glycan structures were also digested, including glycopeptides from pectate lyase, EPG I, and pectin methylesterase from Aspergillus niger. Copyright © 2001 John Wiley & Sons, Ltd.
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on target exoglycosidase Digestions maldi ms for determining the primary structures of carbohydrate chains
Analytical Chemistry, 1999Co-Authors: Jennifer Colangelo, Ron OrlandoAbstract:One method used to determine the primary sequence of oligosaccharides is to digest them with exoglycosidases and analyze the resulting Digestion products by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Previous research has demonstrated that these Digestions can be performed on the MALDI target. However, the procedure requires the sample to be incubated at elevated temperatures, and complete Digestion requires a few hours. We demonstrate new conditions that permit exoglycosidase Digestions to be performed on the MALDI target at room temperature within 30 min. Oligosaccharide standards were digested with one or more exoglycosidases to show that the enzymes retain their activity and specificity under these new reaction conditions. Using this method, the primary sequences of carbohydrate chains can be determined in a relatively short amount of time.
Chenxi Li - One of the best experts on this subject based on the ideXlab platform.
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enhanced biogas production from anaerobic co Digestion of municipal wastewater treatment sludge and fat oil and grease fog by a modified two stage thermophilic digester system with selected thermo chemical pre treatment
Renewable Energy, 2015Co-Authors: Chenxi Li, Pascale Champagne, Bruce C AndersonAbstract:Anaerobic co-Digestions with fat, oil and grease (FOG) were investigated in two-stage thermophilic (55 °C) semi-continuous flow co-Digestion systems. One two-stage co-Digestion system (System I) was modified to incorporate a thermo-chemical pre-treatment of pH = 10 at 55 °C, which was the best pre-treatment condition for FOG co-Digestion identified during laboratory-scale biochemical methane potential (BMP) testing. The other two-stage co-Digestion system (System II) was operated without a pre-treatment process. The anaerobic digester of each Digestion system had a hydraulic retention time (HRT) of 24 days. An organic loading rate (OLR) of 1.83 ± 0.09 g TVS/L·d was applied to each Digestion system. It was found that System I effectively enhanced biogas production as the thermo-chemical pre-treatment improved the substrate hydrolysis including increased COD solubilization and VFA concentrations. Overall, the modified System I yielded a 25.14 ± 2.14 L/d biogas production rate, which was substantially higher than the 18.73 ± 1.11 L/d obtained in the System II.
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biogas production performance of mesophilic and thermophilic anaerobic co Digestion with fat oil and grease in semi continuous flow digesters effects of temperature hydraulic retention time and organic loading rate
Environmental Technology, 2013Co-Authors: Chenxi Li, Pascale Champagne, Bruce C AndersonAbstract:Anaerobic co-Digestions with fat, oil, and grease (FOG) were investigated in semi-continuous flow digesters under various operating conditions. The effects of hydraulic retention times (HRTs) of 12 and 24 days, organic loading rates (OLRs) between 1.19 and 8.97 g TVS/Ld, and Digestion temperatures of 37°C and 55°C on biogas production were evaluated. It was proposed that, compared to anaerobic Digestion with wastewater treatment plant sludge (primary raw sludge), semi-continuous flow anaerobic co-Digestion with FOG could effectively enhance biogas and methane production. Thermophilic (55°C) co-Digestions exhibited higher biogas production and degradation of organics than mesophilic co-Digestions. The best biogas production rate of 17.4±0.86 L/d and methane content 67.9±1.46% was obtained with a thermophilic co-Digestion at HRT=24 days and OLR=2.43±0.15 g TVS/Ld. These were 32.8% and 7.10% higher than the respective values from the mesophilic co-Digestion under similar operating conditions.
-
effects of ultrasonic and thermo chemical pre treatments on methane production from fat oil and grease fog and synthetic kitchen waste kw in anaerobic co Digestion
Bioresource Technology, 2013Co-Authors: Chenxi Li, Pascale Champagne, Bruce C AndersonAbstract:Abstract The effects of ultrasonic and thermo-chemical pre-treatments on the methane production potential of anaerobic co-Digestion with synthetic kitchen waste (KW) or fat, oil and grease (FOG) were investigated. Non-linear regressions were fitted to accurately assess and compare the methane production from co-Digestion under the various pre-treatment conditions and to achieve representative simulations and predictions. Ultrasonic pre-treatment was not found to improve methane production effectively from either FOG co-Digestion or KW co-Digestions. Thermo-chemical pre-treatment could increase methane production yields from both FOG and KW co-Digestions. COD solubilization was found to effectively represent the effects of pre-treatment. A comprehensive evaluation indicated that the thermo-chemical pre-treatments of pH = 10, 55 °C and pH = 8, 55 °C provided the best conditions to increase methane production from FOG and KW co-Digestions, respectively. The most effective enhancement of biogas production (288 ± 0.85 mL CH4/g TVS) was achieved from thermo-chemically pre-treated FOG co-Digestion, which was 9.9 ± 1.5% higher than FOG co-Digestion without thermo-chemical pre-treatment.
-
evaluating and modeling biogas production from municipal fat oil and grease and synthetic kitchen waste in anaerobic co Digestions
Bioresource Technology, 2011Co-Authors: Chenxi Li, Pascale Champagne, Bruce C AndersonAbstract:The feasibility of using synthetic kitchen waste (KW) and fat, oil, and grease (FOG) as co-substrates in the anaerobic Digestion of waste activated sludge (WAS) was investigated using two series of biochemical methane potential (BMP) tests. Ranges of ideal substrate to inoculum (S/I) ratio were determined for the FOG (0.25–0.75) and KW (0.80–1.26) as single substrates in the first experiment. The second experiment, which estimated the methane production performances of FOG and KW as co-substrates for WAS co-Digestion, was conducted based on the optimal parameters selected from the results of the first experiment. Results indicated that co-Digestions with FOG and KW enhanced methane production from 117 ± 2.02 mL/gTVS (with only WAS) to 418 ± 13.7 mL/gTVS and 324 ± 4.11 mL/gTVS, respectively. FOG exhibited more biogas production than KW as co-substrate. Non-linear regression results showed that co-substrate addition shortened the lag phases of organic biodegradation from 81.8 (with only WAS) to 28.3 h with FOG and 3.90 h with KW.
