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Cedric J Simon - One of the best experts on this subject based on the ideXlab platform.
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Digestive Enzyme response to natural and formulated diets in cultured juvenile spiny lobster jasus edwardsii
Aquaculture, 2009Co-Authors: Cedric J SimonAbstract:A better understanding of the nutritional requirements and Digestive processes are essential for improving the consumption and growth of spiny lobsters on formulated diets used in commercial culture. Differences in the enzymatic Digestive response (i.e., post-prandial changes in Digestive Enzyme activity, Digestive fluid pH and Digestive gland structure) of juvenile Jasus edwardsii fed either a natural (mussel flesh) or a formulated diet were investigated. Digestive Enzyme activities (total protease, trypsin, α-amylase and α-glucosidase) and soluble protein concentration in the foregut after a single feeding event for the two dietary treatments displayed a common peak at 4 h, and a second peak at 18 h post-prandial for the mussel fed lobsters only. In lobsters fed the formulated diet, the lumen size of the Digestive gland tubules was 88% larger, the number of B-cells per tubule at 18 h post-feeding was 269% higher, and the pH of the Digestive gland was lower (6.20 versus 6.34) than in lobsters fed mussel flesh. These results indicated an intensified intracellular digestion in the Digestive gland on the formulated diet that could have played a role in the poor appetite revival (>18 h) exhibited on this diet. Rearing of juvenile lobsters on the formulated diet for 6 months resulted in a marked decrease in the Digestive capacity (i.e., total and specific Enzyme activity of the foregut and Digestive gland) and nutritional condition of lobsters. Overall, these results suggest that difficulty in the Digestive processing of formulated feeds may help to explain the bottlenecks encountered in developing more effective formulated diets for juvenile spiny lobster culture. Improvements in the dissolution of dietary ingredients upon entering the foregut, and in the digestibility of dietary carbohydrate sources, may assist in further improving the performance of formulated diets for lobsters.
Shan Xiao - One of the best experts on this subject based on the ideXlab platform.
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effect of potential probiotic rhodotorula benthica d30 on the growth performance Digestive Enzyme activity and immunity in juvenile sea cucumber apostichopus japonicus
Fish & Shellfish Immunology, 2015Co-Authors: Jihui Wang, Liuqun Zhao, Jinfeng Liu, Han Wang, Shan XiaoAbstract:Abstract The effects of dietary addition of yeast Rhodotorula benthica (R. benthica) D30 which isolated from local sea mud at levels of 0 (control), 105, 106 and 107 CFU/g feed on the growth performance, Digestive Enzyme activity, immunity and disease resistance of juvenile sea cucumber Apostichopus japonicus were investigated. It was shown that dietary addition of R. benthica D30 significantly increased the growth rates of sea cucumbers (p 0.05). It was observed that adding R. benthica D30 could significantly decrease the cumulative mortality of sea cucumbers. The present study demonstrated that dietary addition of R. benthica D30 could increase growth performance and some Digestive Enzyme activities, improve immunity and disease resistance of A. japonicus. And the medium (106 CFU) and high (107 CFU) additional levels showed better effects. It suggests that yeast R. benthica D30 could be a good probiotic for aquaculture.
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effect of potential probiotic rhodotorula benthica d30 on the growth performance Digestive Enzyme activity and immunity in juvenile sea cucumber apostichopus japonicus
Fish & Shellfish Immunology, 2015Co-Authors: Jihui Wang, Liuqun Zhao, Jinfeng Liu, Han Wang, Shan XiaoAbstract:Abstract The effects of dietary addition of yeast Rhodotorula benthica ( R. benthica ) D30 which isolated from local sea mud at levels of 0 (control), 10 5 , 10 6 and 10 7 CFU/g feed on the growth performance, Digestive Enzyme activity, immunity and disease resistance of juvenile sea cucumber Apostichopus japonicus were investigated. It was shown that dietary addition of R. benthica D30 significantly increased the growth rates of sea cucumbers ( p R. benthica D30 at the level of 10 7 CFU significantly increased the lysozyme, phagocytic and total nitric oxide synthase activity of A. japonicus ( p R. benthica D30 at the level of 10 6 CFU. Whereas adding R. benthica D30 to diet had no significant effects on the total coelomocyte counts and acid phosphatase activity of A. japonicus ( p > 0.05). It was observed that adding R. benthica D30 could significantly decrease the cumulative mortality of sea cucumbers. The present study demonstrated that dietary addition of R. benthica D30 could increase growth performance and some Digestive Enzyme activities, improve immunity and disease resistance of A. japonicus . And the medium (10 6 CFU) and high (10 7 CFU) additional levels showed better effects. It suggests that yeast R. benthica D30 could be a good probiotic for aquaculture.
Liuqun Zhao - One of the best experts on this subject based on the ideXlab platform.
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effect of potential probiotic rhodotorula benthica d30 on the growth performance Digestive Enzyme activity and immunity in juvenile sea cucumber apostichopus japonicus
Fish & Shellfish Immunology, 2015Co-Authors: Jihui Wang, Liuqun Zhao, Jinfeng Liu, Han Wang, Shan XiaoAbstract:Abstract The effects of dietary addition of yeast Rhodotorula benthica (R. benthica) D30 which isolated from local sea mud at levels of 0 (control), 105, 106 and 107 CFU/g feed on the growth performance, Digestive Enzyme activity, immunity and disease resistance of juvenile sea cucumber Apostichopus japonicus were investigated. It was shown that dietary addition of R. benthica D30 significantly increased the growth rates of sea cucumbers (p 0.05). It was observed that adding R. benthica D30 could significantly decrease the cumulative mortality of sea cucumbers. The present study demonstrated that dietary addition of R. benthica D30 could increase growth performance and some Digestive Enzyme activities, improve immunity and disease resistance of A. japonicus. And the medium (106 CFU) and high (107 CFU) additional levels showed better effects. It suggests that yeast R. benthica D30 could be a good probiotic for aquaculture.
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effect of potential probiotic rhodotorula benthica d30 on the growth performance Digestive Enzyme activity and immunity in juvenile sea cucumber apostichopus japonicus
Fish & Shellfish Immunology, 2015Co-Authors: Jihui Wang, Liuqun Zhao, Jinfeng Liu, Han Wang, Shan XiaoAbstract:Abstract The effects of dietary addition of yeast Rhodotorula benthica ( R. benthica ) D30 which isolated from local sea mud at levels of 0 (control), 10 5 , 10 6 and 10 7 CFU/g feed on the growth performance, Digestive Enzyme activity, immunity and disease resistance of juvenile sea cucumber Apostichopus japonicus were investigated. It was shown that dietary addition of R. benthica D30 significantly increased the growth rates of sea cucumbers ( p R. benthica D30 at the level of 10 7 CFU significantly increased the lysozyme, phagocytic and total nitric oxide synthase activity of A. japonicus ( p R. benthica D30 at the level of 10 6 CFU. Whereas adding R. benthica D30 to diet had no significant effects on the total coelomocyte counts and acid phosphatase activity of A. japonicus ( p > 0.05). It was observed that adding R. benthica D30 could significantly decrease the cumulative mortality of sea cucumbers. The present study demonstrated that dietary addition of R. benthica D30 could increase growth performance and some Digestive Enzyme activities, improve immunity and disease resistance of A. japonicus . And the medium (10 6 CFU) and high (10 7 CFU) additional levels showed better effects. It suggests that yeast R. benthica D30 could be a good probiotic for aquaculture.
D. J. Johnston - One of the best experts on this subject based on the ideXlab platform.
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Ontogenetic changes in Digestive Enzyme activity of the spiny lobster, Jasus edwardsii (Decapoda; Palinuridae)
Marine Biology, 2003Co-Authors: D. J. JohnstonAbstract:Digestive Enzyme profiles of puerulus, post-puerulus, juvenile and adult stages of the spiny lobster Jasus edwardsii Hutton were determined in order to identify ontogenetic changes in Digestive capabilities and assess capacity to use dietary components and to exploit diets to meet nutritional requirements. Juvenile and adult lobsters exhibited a diverse range of Enzymes, suggesting they can exploit varied diets. Proteolytic activity of trypsin, chymotrypsin and carboxypeptidase A (1.86–3.70 U mg^−1 Digestive gland protein) was significantly higher than carbohydrase activity of amylase, α-glucosidase, cellulase and chitinase (0.0014–0.38 U mg^−1 Digestive gland protein), thus showing that dietary protein was more important than carbohydrate and that the lobster is carnivorous. These conclusions are consistent with other studies that found spiny lobsters to feed predominantly on crabs, bivalves, ophiuroids and sponges. Lipase activity (0.371 U mg^−1 Digestive gland protein) was also relatively high, thus showing the importance of dietary lipid. Total activities (units per Digestive gland) of every Enzyme assayed increased significantly with lobster carapace length. There were few significant ontogenetic trends in specific Enzyme activity (U mg^−1 Digestive gland protein). Amylase and laminarinase specific activity was significantly higher in small lobsters than large lobsters (regression analyses, respectively, F _(1,23)=9.84, P =0.005; F _(1,11)=19.65, P =0.001), suggesting that carbohydrates including laminarin are more important in the diet of small juveniles. Trypsin, amylase and lipase activities were detected in all non-feeding puerulus stages, suggesting that feeding is not a cue for Digestive Enzyme production in J . edwardsii . Significant variations in total and specific activities of amylase ( F _(1,3)=14.2, P =0.00; F _(1,3)=14.2, P =0.00) and trypsin ( F _(1,3)=8.8, P =0.00; F _(1,3)=21.41, P =0.00) and a declining trend in lipase total activity between non-feeding puerulus stages suggests that they may be hydrolysing endogenous energy reserves to sustain their onshore swimming activity prior to settlement. Profiles suggest carbohydrate and lipid are utilised first, followed by protein. Consistently high levels of lipase in all puerulus stages (0.24–0.7 U Digestive gland^−1; P >0.05) confirm the importance of lipid as a major energy substrate.
Mansour Torfi Mozanzadeh - One of the best experts on this subject based on the ideXlab platform.
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Growth Performance, Hemato-Immunological Responses, and Digestive Enzyme Activities in Silvery-Black Porgy (Sparidentex hasta) Fed Dietary Bovine Lactoferrin
Probiotics and Antimicrobial Proteins, 2018Co-Authors: Esmaeil Pagheh, Jasem G. Marammazi, Naser Agh, Farzaneh Nouri, Abolfazl Sepahdari, Enric Gisbert, Mansour Torfi MozanzadehAbstract:An 8-week study was conducted to evaluate three different diets supplemented with bovine lactoferrin (LF) at 0 (control), 800, and 1200 mg LF kg^−1 diet on somatic growth, hemato-immunological parameters, antioxidant status, and Digestive Enzyme activities in silvery-black porgy ( Sparidentex hasta ) juveniles. Fish fed the 800 mg LF kg^−1 diet had higher growth performance and feed utilization parameters than the other groups. Hematological and liver antioxidant parameters were not affected by dietary LF supplementation. Fish fed the 800 mg LF kg^−1 diet had higher plasma lysozyme activity values than the other groups. Total protease activity was higher in fish fed LF-supplemented diets than the control group. Results indicated that diet supplemented with 800 mg kg^−1 for 8 weeks enhanced somatic growth performance, lysozyme activity, and proteolytic Digestive Enzyme activities in S. hasta , as well as improving feed efficiency parameters like the protein efficiency and feed conversion ratios.