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Bozena Muszynska - One of the best experts on this subject based on the ideXlab platform.
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determining the amount of potentially bioavailable phenolic compounds and bioelements in edible mushroom mycelia of agaricus bisporus cantharellus cibarius and lentinula edodes
Food Chemistry, 2021Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Beata Ostachowicz, Kornelia Szczurek, Agata Fijalkowska, Bozena MuszynskaAbstract:Abstract Release of bioelements and phenolic compounds from edible mushrooms (Agaricus bisporus, Cantharellus cibarius, and Lentinula edodes) enriched with zinc, selenium, l -phenylalanine, alone and as a mixture was examined using a simulated human gastrointestinal digestion method. Due to the extensive amount of data obtained, in order to interpret them more precisely in the work, the methods of chemometric analysis (Cluster Analysis—CA and Principal Compenent Analysis—PCA) were additionally applied. The results showed mycelium of L. edodes has the best health-promoting properties and addition of mixture to the media increased significantly the synthesis of p-hydroxybenzoic and protocatechuic acid (267 and 16.3 mg/100 g d.w.). After extraction into artificial Digestive Juices, 97.4 mg/100 g d.w. p-hydroxybenzoic acid and 15.6 mg/100 g d.w. of protocatechuic acid were released. The greatest amounts of Se and Zn were extracted from enriched A. bisporus mycelium (32.3 and 342 mg/100 g d.w., respectively). This study confirmed that mycelium might prevent nutritional deficiencies in the diet through use of functional foods.
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correction to lentinula edodes as a source of bioelements released into artificial Digestive Juices and potential anti inflammatory material
Biological Trace Element Research, 2020Co-Authors: Bozena Muszynska, Agata Krakowska, Katarzyna Kala, Beata Ostachowicz, Anna Wlodarczyk, Joanna Gdulaargasinska, Piotr SuchockiAbstract:Lentinula edodes (shiitake), an edible and medicinal mushroom, was chosen for this study with the aim of evaluating the possibility of release of bioelements into artificial Digestive Juices and analyzing the anti-inflammatory properties. The extracts were prepared from fruiting bodies and biomass enriched with copper (Cu), zinc (Zn), and selenium (Se). The content of bioelements was analyzed by total reflection X-ray fluorescence method. Relatively low content of elements was observed in the fruiting bodies: Cu–1.6, Zn–7.6, and Se–0.12 mg/100 g d.w. compared to mycelial cultures. The anti-inflammatory properties were evaluated in RAW 264.7 cells. Based on the levels of cyclooxygenase 2 protein, nuclear factor erythroid 2-related factor 2, and peroxisome proliferator-activated receptor γ determined using Western blot technique, it was found that the addition of bioelements enhanced the anti-inflammatory properties of mycelium. This indicates that L. edodes cultured on a suitable medium may be used as a potential component of anti-inflammatory products.
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kinetics of extracted bioactive components from mushrooms in artificial Digestive Juices
International Journal of Food Properties, 2017Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Wlodzimierz Opoka, Katarzyna Sulkowskaziaja, Witold Reczynski, Bozena MuszynskaAbstract:ABSTRACTThis article presents a study on fruiting bodies of Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Leccinum scabrum, Suillus bovinus, Suillus luteus, Pleurotus ostreatus, Tricholoma equestre, Armillaria mellea, Agaricus bisporus, and Auricularia polytricha. The amounts of their health-promoting bioelements and physiologically significant indole and phenolic compounds were determined. The significance of edible mushrooms in the human diet and in disease prevention were also established. The high-performance liquid chromatography was used to assess the contents of indole compounds and phenolic acids. The atomic absorption spectroscopy was applied to determine bioelements. In all samples, the content of 5-hydroxy-L-tryptophan was studied. The highest extraction amount of serotonin was found for P. ostreatus species. Phenolic compounds were determined in the conditions described in the present study for the first time. Additionally, protocatechuic, p-hydroxybenzoic, syrin...
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chemopreventive and anticancer activities of bacopa monnieri extracted from artificial Digestive Juices
Natural Product Communications, 2017Co-Authors: Paulina Koczurkiewicz, Agnieszka Szewczyk, Maciej łojewski, Kamil Piska, Marta Michalik, Katarzyna Wojcikpszczola, Patrycja Halaszuk, Elzbieta Pekala, Bozena MuszynskaAbstract:Bacopa monnieri (L.) Pennell (water hyssop) from the Scrophulariaceae family represents one of the best known plants of the Ayurveda system, with reported procognitive, antioxidant, anti-inflammato...
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in vitro culture of boletus badius as a source of indole compounds and zinc released in artificial Digestive Juices
Food Science and Biotechnology, 2016Co-Authors: Katarzyna Kala, Wlodzimierz Opoka, Anna Maślanka, Katarzyna Sulkowskaziaja, Jacek Rojowski, Bozena MuszynskaAbstract:The objective of this study was to obtain the in vitro cultures of Boletus badius under controlled conditions and investigate the release of indole compounds and zinc from the mycelium of B. badius to artificial Digestive Juices under conditions similar to those in the human gastrointestinal tract. Biomass was obtained from cultures grown using both only the Oddoux medium as well as the same medium with added zinc hydroaspartate and zinc sulfate. The release of 5-hydroxy-L-tryptophan, L-tryptophan, and serotonin from the B. badius biomass extracts to the artificial Digestive Juices was determined. Differential pulse anodic stripping voltammetry was used to demonstrate that zinc is released from each of the extracted materials. The total amount of zinc in the materials under study was estimated to be between 7.12 and 44.15 mg/100 g dry weight. It was demonstrated that in vitro cultures of B. badius grown using appropriately selected media may supplement zinc and indole compounds.
Katarzyna Kala - One of the best experts on this subject based on the ideXlab platform.
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determining the amount of potentially bioavailable phenolic compounds and bioelements in edible mushroom mycelia of agaricus bisporus cantharellus cibarius and lentinula edodes
Food Chemistry, 2021Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Beata Ostachowicz, Kornelia Szczurek, Agata Fijalkowska, Bozena MuszynskaAbstract:Abstract Release of bioelements and phenolic compounds from edible mushrooms (Agaricus bisporus, Cantharellus cibarius, and Lentinula edodes) enriched with zinc, selenium, l -phenylalanine, alone and as a mixture was examined using a simulated human gastrointestinal digestion method. Due to the extensive amount of data obtained, in order to interpret them more precisely in the work, the methods of chemometric analysis (Cluster Analysis—CA and Principal Compenent Analysis—PCA) were additionally applied. The results showed mycelium of L. edodes has the best health-promoting properties and addition of mixture to the media increased significantly the synthesis of p-hydroxybenzoic and protocatechuic acid (267 and 16.3 mg/100 g d.w.). After extraction into artificial Digestive Juices, 97.4 mg/100 g d.w. p-hydroxybenzoic acid and 15.6 mg/100 g d.w. of protocatechuic acid were released. The greatest amounts of Se and Zn were extracted from enriched A. bisporus mycelium (32.3 and 342 mg/100 g d.w., respectively). This study confirmed that mycelium might prevent nutritional deficiencies in the diet through use of functional foods.
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correction to lentinula edodes as a source of bioelements released into artificial Digestive Juices and potential anti inflammatory material
Biological Trace Element Research, 2020Co-Authors: Bozena Muszynska, Agata Krakowska, Katarzyna Kala, Beata Ostachowicz, Anna Wlodarczyk, Joanna Gdulaargasinska, Piotr SuchockiAbstract:Lentinula edodes (shiitake), an edible and medicinal mushroom, was chosen for this study with the aim of evaluating the possibility of release of bioelements into artificial Digestive Juices and analyzing the anti-inflammatory properties. The extracts were prepared from fruiting bodies and biomass enriched with copper (Cu), zinc (Zn), and selenium (Se). The content of bioelements was analyzed by total reflection X-ray fluorescence method. Relatively low content of elements was observed in the fruiting bodies: Cu–1.6, Zn–7.6, and Se–0.12 mg/100 g d.w. compared to mycelial cultures. The anti-inflammatory properties were evaluated in RAW 264.7 cells. Based on the levels of cyclooxygenase 2 protein, nuclear factor erythroid 2-related factor 2, and peroxisome proliferator-activated receptor γ determined using Western blot technique, it was found that the addition of bioelements enhanced the anti-inflammatory properties of mycelium. This indicates that L. edodes cultured on a suitable medium may be used as a potential component of anti-inflammatory products.
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kinetics of extracted bioactive components from mushrooms in artificial Digestive Juices
International Journal of Food Properties, 2017Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Wlodzimierz Opoka, Katarzyna Sulkowskaziaja, Witold Reczynski, Bozena MuszynskaAbstract:ABSTRACTThis article presents a study on fruiting bodies of Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Leccinum scabrum, Suillus bovinus, Suillus luteus, Pleurotus ostreatus, Tricholoma equestre, Armillaria mellea, Agaricus bisporus, and Auricularia polytricha. The amounts of their health-promoting bioelements and physiologically significant indole and phenolic compounds were determined. The significance of edible mushrooms in the human diet and in disease prevention were also established. The high-performance liquid chromatography was used to assess the contents of indole compounds and phenolic acids. The atomic absorption spectroscopy was applied to determine bioelements. In all samples, the content of 5-hydroxy-L-tryptophan was studied. The highest extraction amount of serotonin was found for P. ostreatus species. Phenolic compounds were determined in the conditions described in the present study for the first time. Additionally, protocatechuic, p-hydroxybenzoic, syrin...
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in vitro culture of boletus badius as a source of indole compounds and zinc released in artificial Digestive Juices
Food Science and Biotechnology, 2016Co-Authors: Katarzyna Kala, Wlodzimierz Opoka, Anna Maślanka, Katarzyna Sulkowskaziaja, Jacek Rojowski, Bozena MuszynskaAbstract:The objective of this study was to obtain the in vitro cultures of Boletus badius under controlled conditions and investigate the release of indole compounds and zinc from the mycelium of B. badius to artificial Digestive Juices under conditions similar to those in the human gastrointestinal tract. Biomass was obtained from cultures grown using both only the Oddoux medium as well as the same medium with added zinc hydroaspartate and zinc sulfate. The release of 5-hydroxy-L-tryptophan, L-tryptophan, and serotonin from the B. badius biomass extracts to the artificial Digestive Juices was determined. Differential pulse anodic stripping voltammetry was used to demonstrate that zinc is released from each of the extracted materials. The total amount of zinc in the materials under study was estimated to be between 7.12 and 44.15 mg/100 g dry weight. It was demonstrated that in vitro cultures of B. badius grown using appropriately selected media may supplement zinc and indole compounds.
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agaricus bisporus and its in vitro culture as a source of indole compounds released into artificial Digestive Juices
Food Chemistry, 2016Co-Authors: Bozena Muszynska, Agata Krakowska, Katarzyna Kala, Katarzyna Sulkowskaziaja, Wlodzimierz OpokaAbstract:The popularity of Agaricus bisporus results not only from the quality of the flavors, but also from the content of many substances of therapeutic properties. This paper presents a study on RP-HPLC determination of the content of indole compounds released from the lyophilized biomass from in vitro cultures of A. bisporus into artificial Digestive Juices at 37°C. A. bisporus in vitro cultures were grown on media enriched with zinc salts. The release of 5-hydroxy-l-tryptophan and l-tryptophan was found in the greatest number of samples. The content of 5-hydroxy-l-tryptophan in the investigated samples ranged from 86.62 to 531 mg/100g d.w. The amount of l-tryptophan was determined within the range of 1.63-4.68 mg/100g d.w. and for melatonin 0.43-0.64 mg/100g d.w. It was demonstrated for the first time that in vitro cultures of A. bisporus release indole compounds in conditions simulating the human Digestive tract.
Agata Krakowska - One of the best experts on this subject based on the ideXlab platform.
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determining the amount of potentially bioavailable phenolic compounds and bioelements in edible mushroom mycelia of agaricus bisporus cantharellus cibarius and lentinula edodes
Food Chemistry, 2021Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Beata Ostachowicz, Kornelia Szczurek, Agata Fijalkowska, Bozena MuszynskaAbstract:Abstract Release of bioelements and phenolic compounds from edible mushrooms (Agaricus bisporus, Cantharellus cibarius, and Lentinula edodes) enriched with zinc, selenium, l -phenylalanine, alone and as a mixture was examined using a simulated human gastrointestinal digestion method. Due to the extensive amount of data obtained, in order to interpret them more precisely in the work, the methods of chemometric analysis (Cluster Analysis—CA and Principal Compenent Analysis—PCA) were additionally applied. The results showed mycelium of L. edodes has the best health-promoting properties and addition of mixture to the media increased significantly the synthesis of p-hydroxybenzoic and protocatechuic acid (267 and 16.3 mg/100 g d.w.). After extraction into artificial Digestive Juices, 97.4 mg/100 g d.w. p-hydroxybenzoic acid and 15.6 mg/100 g d.w. of protocatechuic acid were released. The greatest amounts of Se and Zn were extracted from enriched A. bisporus mycelium (32.3 and 342 mg/100 g d.w., respectively). This study confirmed that mycelium might prevent nutritional deficiencies in the diet through use of functional foods.
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correction to lentinula edodes as a source of bioelements released into artificial Digestive Juices and potential anti inflammatory material
Biological Trace Element Research, 2020Co-Authors: Bozena Muszynska, Agata Krakowska, Katarzyna Kala, Beata Ostachowicz, Anna Wlodarczyk, Joanna Gdulaargasinska, Piotr SuchockiAbstract:Lentinula edodes (shiitake), an edible and medicinal mushroom, was chosen for this study with the aim of evaluating the possibility of release of bioelements into artificial Digestive Juices and analyzing the anti-inflammatory properties. The extracts were prepared from fruiting bodies and biomass enriched with copper (Cu), zinc (Zn), and selenium (Se). The content of bioelements was analyzed by total reflection X-ray fluorescence method. Relatively low content of elements was observed in the fruiting bodies: Cu–1.6, Zn–7.6, and Se–0.12 mg/100 g d.w. compared to mycelial cultures. The anti-inflammatory properties were evaluated in RAW 264.7 cells. Based on the levels of cyclooxygenase 2 protein, nuclear factor erythroid 2-related factor 2, and peroxisome proliferator-activated receptor γ determined using Western blot technique, it was found that the addition of bioelements enhanced the anti-inflammatory properties of mycelium. This indicates that L. edodes cultured on a suitable medium may be used as a potential component of anti-inflammatory products.
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Bioaccessibility of phenolic compounds, lutein, and bioelements of preparations containing Chlorella vulgaris in artificial Digestive Juices
Journal of Applied Phycology, 2017Co-Authors: Bożena Muszyńska, Agata Krakowska, Barbara Jękot, Łukasz Zimmer, Katarzyna Sułkowska-ziaja, Agnieszka Szewczyk, J Lazur, Ewa Poleszak, Włodzimierz OpokaAbstract:Chlorella vulgaris Beijerinck is a spherical, green alga belonging to the genus Chlorella and family Chlorellaceae. It has high nutritional value and shows multiple biological effects. Dietary supplements that contain extracts of C. vulgaris are sold in the form of tablets, capsules, powders, and aqueous solutions. To the best of our knowledge, this is the first study to determine the content of bioelements (zinc, iron, and magnesium), phenolic compounds, and lutein before and after incubation with artificial Digestive Juices from preparations containing C. vulgaris. In this study, we used commercial preparations in the form of powder and tablets. The samples were incubated in artificial gastric juice and then in artificial intestinal juice for 30 and 90 min. The contents of bioelements were determined by using the flame atomic absorption spectrometric method. Lutein and phenolic compounds were analyzed by high-pressure liquid chromatography. We also aimed to evaluate the quality of chlorella-containing formulations by using the methods described in the European Pharmacopoeia 8th edition. According to the results, the preparations containing C. vulgaris demonstrated the presence of phenolic compounds and lutein. Therefore, daily supplementation of preparations containing C. vulgaris substantiates its usefulness for humans. The qualitative composition of the examined organic substances and bioelements was found to be in accordance with the manufacturer’s declarations on the packaging containing C. vulgaris compared with the control samples; however, the contents of bioelements were found to be negligible after incubation with artificial Digestive Juices. This shows that the examined preparations containing C. vulgaris are not good sources of bioelements such as zinc, iron, or magnesium.
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kinetics of extracted bioactive components from mushrooms in artificial Digestive Juices
International Journal of Food Properties, 2017Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Wlodzimierz Opoka, Katarzyna Sulkowskaziaja, Witold Reczynski, Bozena MuszynskaAbstract:ABSTRACTThis article presents a study on fruiting bodies of Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Leccinum scabrum, Suillus bovinus, Suillus luteus, Pleurotus ostreatus, Tricholoma equestre, Armillaria mellea, Agaricus bisporus, and Auricularia polytricha. The amounts of their health-promoting bioelements and physiologically significant indole and phenolic compounds were determined. The significance of edible mushrooms in the human diet and in disease prevention were also established. The high-performance liquid chromatography was used to assess the contents of indole compounds and phenolic acids. The atomic absorption spectroscopy was applied to determine bioelements. In all samples, the content of 5-hydroxy-L-tryptophan was studied. The highest extraction amount of serotonin was found for P. ostreatus species. Phenolic compounds were determined in the conditions described in the present study for the first time. Additionally, protocatechuic, p-hydroxybenzoic, syrin...
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agaricus bisporus and its in vitro culture as a source of indole compounds released into artificial Digestive Juices
Food Chemistry, 2016Co-Authors: Bozena Muszynska, Agata Krakowska, Katarzyna Kala, Katarzyna Sulkowskaziaja, Wlodzimierz OpokaAbstract:The popularity of Agaricus bisporus results not only from the quality of the flavors, but also from the content of many substances of therapeutic properties. This paper presents a study on RP-HPLC determination of the content of indole compounds released from the lyophilized biomass from in vitro cultures of A. bisporus into artificial Digestive Juices at 37°C. A. bisporus in vitro cultures were grown on media enriched with zinc salts. The release of 5-hydroxy-l-tryptophan and l-tryptophan was found in the greatest number of samples. The content of 5-hydroxy-l-tryptophan in the investigated samples ranged from 86.62 to 531 mg/100g d.w. The amount of l-tryptophan was determined within the range of 1.63-4.68 mg/100g d.w. and for melatonin 0.43-0.64 mg/100g d.w. It was demonstrated for the first time that in vitro cultures of A. bisporus release indole compounds in conditions simulating the human Digestive tract.
Wlodzimierz Opoka - One of the best experts on this subject based on the ideXlab platform.
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kinetics of extracted bioactive components from mushrooms in artificial Digestive Juices
International Journal of Food Properties, 2017Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Wlodzimierz Opoka, Katarzyna Sulkowskaziaja, Witold Reczynski, Bozena MuszynskaAbstract:ABSTRACTThis article presents a study on fruiting bodies of Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Leccinum scabrum, Suillus bovinus, Suillus luteus, Pleurotus ostreatus, Tricholoma equestre, Armillaria mellea, Agaricus bisporus, and Auricularia polytricha. The amounts of their health-promoting bioelements and physiologically significant indole and phenolic compounds were determined. The significance of edible mushrooms in the human diet and in disease prevention were also established. The high-performance liquid chromatography was used to assess the contents of indole compounds and phenolic acids. The atomic absorption spectroscopy was applied to determine bioelements. In all samples, the content of 5-hydroxy-L-tryptophan was studied. The highest extraction amount of serotonin was found for P. ostreatus species. Phenolic compounds were determined in the conditions described in the present study for the first time. Additionally, protocatechuic, p-hydroxybenzoic, syrin...
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in vitro culture of boletus badius as a source of indole compounds and zinc released in artificial Digestive Juices
Food Science and Biotechnology, 2016Co-Authors: Katarzyna Kala, Wlodzimierz Opoka, Anna Maślanka, Katarzyna Sulkowskaziaja, Jacek Rojowski, Bozena MuszynskaAbstract:The objective of this study was to obtain the in vitro cultures of Boletus badius under controlled conditions and investigate the release of indole compounds and zinc from the mycelium of B. badius to artificial Digestive Juices under conditions similar to those in the human gastrointestinal tract. Biomass was obtained from cultures grown using both only the Oddoux medium as well as the same medium with added zinc hydroaspartate and zinc sulfate. The release of 5-hydroxy-L-tryptophan, L-tryptophan, and serotonin from the B. badius biomass extracts to the artificial Digestive Juices was determined. Differential pulse anodic stripping voltammetry was used to demonstrate that zinc is released from each of the extracted materials. The total amount of zinc in the materials under study was estimated to be between 7.12 and 44.15 mg/100 g dry weight. It was demonstrated that in vitro cultures of B. badius grown using appropriately selected media may supplement zinc and indole compounds.
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agaricus bisporus and its in vitro culture as a source of indole compounds released into artificial Digestive Juices
Food Chemistry, 2016Co-Authors: Bozena Muszynska, Agata Krakowska, Katarzyna Kala, Katarzyna Sulkowskaziaja, Wlodzimierz OpokaAbstract:The popularity of Agaricus bisporus results not only from the quality of the flavors, but also from the content of many substances of therapeutic properties. This paper presents a study on RP-HPLC determination of the content of indole compounds released from the lyophilized biomass from in vitro cultures of A. bisporus into artificial Digestive Juices at 37°C. A. bisporus in vitro cultures were grown on media enriched with zinc salts. The release of 5-hydroxy-l-tryptophan and l-tryptophan was found in the greatest number of samples. The content of 5-hydroxy-l-tryptophan in the investigated samples ranged from 86.62 to 531 mg/100g d.w. The amount of l-tryptophan was determined within the range of 1.63-4.68 mg/100g d.w. and for melatonin 0.43-0.64 mg/100g d.w. It was demonstrated for the first time that in vitro cultures of A. bisporus release indole compounds in conditions simulating the human Digestive tract.
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determination of zinc ii ions released into artificial Digestive Juices from culinary medicinal button mushroom agaricus bisporus agaricomycetidae biomass of in vitro cultures using an anodic stripping voltammetry method
International Journal of Medicinal Mushrooms, 2016Co-Authors: Katarzyna Kala, Bozena Muszynska, M Zajac, Remigiusz Krezalek, Wlodzimierz OpokaAbstract:Zinc is one of those microelements that are essential for the proper functioning of the human body and must be supplemented in our food at a daily dose of 15 mg. It is well known that mushrooms accumulate elements; thus, in order to determine the extent of accumulation and the level of zinc released from mushrooms, in vitro cultures of Agaricus bisporus were established. The cultures were run on a modified Oddoux medium (a control culture) as well as on the same medium with the addition of zinc hydroaspartate (100 and 200 mg/L) and zinc sulfate (87.23 and 174.47 mg/L). These compounds were chosen to help estimate which form, organic or inorganic, results in a better assimilation of zinc(II) ions by biomass. As the next step, the level of zinc(II) ions released from the lyophilized biomass of in vitro cultures to the Digestive Juices, under thermal conditions of the human body (37°C), was determined. For this purpose, artificial Digestive Juices, imitating the composition of human Digestive Juices, were used. For determination of zinc(II) ions in the Digestive tract, an anodic stripping voltammetry method was employed. The amount of zinc released into artificial saliva over 1 minute varied from 0.15 mg/100 g d.w. in the control culture to 2.35 mg/100 g d.w. in the biomass in the medium to which 200 mg/L zinc hydroaspartate had been added. Values were higher in gastric juice and depended on incubation time (2.66 to 30.63 mg/100 g d.w.). In intestinal juice, the highest value of the released zinc grew to 24.20 mg/100 g d.w. (biomass of A. bisporus in vitro cultures in medium with the addition of 200 mg/L zinc hydroaspartate). Total average amount of zinc released into artificial Digestive Juices was the highest (56.26 mg/100 g d.w.) from A. bisporus biomass of in vitro cultures in the medium to which 200 mg/L zinc hydroaspartate had been added.
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popular species of edible mushrooms as a good source of zinc to be released to artificial Digestive Juices
Journal of Physiology and Pharmacology, 2015Co-Authors: M Zajac, Bozena Muszynska, Katarzyna Kala, A Sikora, Wlodzimierz OpokaAbstract:Because fruiting bodies of edible mushrooms accumulate elements very effectively, in this study for the first time we aimed at determining the degree of the release of zinc(II) ions to artificial Digestive Juices imitating the human gastrointestinal tract from freeze-dried popular edible mushroom fruiting bodies, such as Agaricus bisporus, Boletus badius and Cantharellus cibarius. For the analysis, anodic stripping voltammetry method was used. The amount of zinc released to artificial saliva within 1 minute ranged from 0.03 to 1.14 mg/100 g d.w. In gastric juice, the amounts were higher and ranged from 0.75 to 2.07 mg/100 g d.w. depending on the incubation time. After incubation of the freeze-dried edible mushroom fruiting bodies for 1 minute in artificial saliva, 15 in artificial gastric juice and then 150 minutes in artificial intestinal juice, it was found that the concentration of the released zinc in artificial intestinal juice was the highest and amounted to 6.44 mg/100 g d.w. The total average amount of zinc released from Boletus badius was the highest and this was estimated at 4.13 mg/100 g d.w. For the remaining two investigated species of A. bisporus and C. cibarius, the total amounts of zinc released into artificial Digestive Juices were only slightly lower and were estimated at 2.23 and 3.29 mg/100 g d.w. on average, respectively. It was demonstrated for the first time that mushrooms release zinc to artificial Digestive Juices imitating conditions in the human Digestive tract and are a good source of this element.
Agnieszka Szewczyk - One of the best experts on this subject based on the ideXlab platform.
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determining the amount of potentially bioavailable phenolic compounds and bioelements in edible mushroom mycelia of agaricus bisporus cantharellus cibarius and lentinula edodes
Food Chemistry, 2021Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Beata Ostachowicz, Kornelia Szczurek, Agata Fijalkowska, Bozena MuszynskaAbstract:Abstract Release of bioelements and phenolic compounds from edible mushrooms (Agaricus bisporus, Cantharellus cibarius, and Lentinula edodes) enriched with zinc, selenium, l -phenylalanine, alone and as a mixture was examined using a simulated human gastrointestinal digestion method. Due to the extensive amount of data obtained, in order to interpret them more precisely in the work, the methods of chemometric analysis (Cluster Analysis—CA and Principal Compenent Analysis—PCA) were additionally applied. The results showed mycelium of L. edodes has the best health-promoting properties and addition of mixture to the media increased significantly the synthesis of p-hydroxybenzoic and protocatechuic acid (267 and 16.3 mg/100 g d.w.). After extraction into artificial Digestive Juices, 97.4 mg/100 g d.w. p-hydroxybenzoic acid and 15.6 mg/100 g d.w. of protocatechuic acid were released. The greatest amounts of Se and Zn were extracted from enriched A. bisporus mycelium (32.3 and 342 mg/100 g d.w., respectively). This study confirmed that mycelium might prevent nutritional deficiencies in the diet through use of functional foods.
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Bioaccessibility of phenolic compounds, lutein, and bioelements of preparations containing Chlorella vulgaris in artificial Digestive Juices
Journal of Applied Phycology, 2017Co-Authors: Bożena Muszyńska, Agata Krakowska, Barbara Jękot, Łukasz Zimmer, Katarzyna Sułkowska-ziaja, Agnieszka Szewczyk, J Lazur, Ewa Poleszak, Włodzimierz OpokaAbstract:Chlorella vulgaris Beijerinck is a spherical, green alga belonging to the genus Chlorella and family Chlorellaceae. It has high nutritional value and shows multiple biological effects. Dietary supplements that contain extracts of C. vulgaris are sold in the form of tablets, capsules, powders, and aqueous solutions. To the best of our knowledge, this is the first study to determine the content of bioelements (zinc, iron, and magnesium), phenolic compounds, and lutein before and after incubation with artificial Digestive Juices from preparations containing C. vulgaris. In this study, we used commercial preparations in the form of powder and tablets. The samples were incubated in artificial gastric juice and then in artificial intestinal juice for 30 and 90 min. The contents of bioelements were determined by using the flame atomic absorption spectrometric method. Lutein and phenolic compounds were analyzed by high-pressure liquid chromatography. We also aimed to evaluate the quality of chlorella-containing formulations by using the methods described in the European Pharmacopoeia 8th edition. According to the results, the preparations containing C. vulgaris demonstrated the presence of phenolic compounds and lutein. Therefore, daily supplementation of preparations containing C. vulgaris substantiates its usefulness for humans. The qualitative composition of the examined organic substances and bioelements was found to be in accordance with the manufacturer’s declarations on the packaging containing C. vulgaris compared with the control samples; however, the contents of bioelements were found to be negligible after incubation with artificial Digestive Juices. This shows that the examined preparations containing C. vulgaris are not good sources of bioelements such as zinc, iron, or magnesium.
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kinetics of extracted bioactive components from mushrooms in artificial Digestive Juices
International Journal of Food Properties, 2017Co-Authors: Katarzyna Kala, Agata Krakowska, Agnieszka Szewczyk, Wlodzimierz Opoka, Katarzyna Sulkowskaziaja, Witold Reczynski, Bozena MuszynskaAbstract:ABSTRACTThis article presents a study on fruiting bodies of Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Leccinum scabrum, Suillus bovinus, Suillus luteus, Pleurotus ostreatus, Tricholoma equestre, Armillaria mellea, Agaricus bisporus, and Auricularia polytricha. The amounts of their health-promoting bioelements and physiologically significant indole and phenolic compounds were determined. The significance of edible mushrooms in the human diet and in disease prevention were also established. The high-performance liquid chromatography was used to assess the contents of indole compounds and phenolic acids. The atomic absorption spectroscopy was applied to determine bioelements. In all samples, the content of 5-hydroxy-L-tryptophan was studied. The highest extraction amount of serotonin was found for P. ostreatus species. Phenolic compounds were determined in the conditions described in the present study for the first time. Additionally, protocatechuic, p-hydroxybenzoic, syrin...
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chemopreventive and anticancer activities of bacopa monnieri extracted from artificial Digestive Juices
Natural Product Communications, 2017Co-Authors: Paulina Koczurkiewicz, Agnieszka Szewczyk, Maciej łojewski, Kamil Piska, Marta Michalik, Katarzyna Wojcikpszczola, Patrycja Halaszuk, Elzbieta Pekala, Bozena MuszynskaAbstract:Bacopa monnieri (L.) Pennell (water hyssop) from the Scrophulariaceae family represents one of the best known plants of the Ayurveda system, with reported procognitive, antioxidant, anti-inflammato...