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Maria Lodovica Gullino - One of the best experts on this subject based on the ideXlab platform.

  • occurence of fusarium equiseti as a contaminant of Diplotaxis tenuifolia seeds
    Journal of Plant Pathology, 2017
    Co-Authors: G Gilardi, Maria Lodovica Gullino, I Pintore, A Garibaldi
    Abstract:

    Fusarium equiseti, the causal agent of leaf spot of both wild [Diplotaxis tenuifolia (L.) DC] and cultivated [Eruca vesicaria (L.) Cav.] rocket, is a new pathogen on these crops, which has increasingly been found on several commercial farms in Italy. In order to investigate the possible transmission of this pathogen through infested seeds, both wild and cultivated rocket seeds, from eight commercially available seed lots, have been collected from four farms suffering from severe field losses. F. equiseti was identified through morphological observations and molecular analysis based on the elongation factor 1 alpha gene (EF-1α). Four out of six seed lot samples of wild rocket were found to be contaminated, while the pathogen was not isolated from the two tested seed lots of E. vesicaria. The highest level of detected infestation was 5 out of 800 non-disinfected Diplotaxis sp. seeds. F. equiseti was not isolated from disinfected seeds. Nine of eleven isolates of F. equiseti obtained from seeds were pathogenic on wild rocket. This work demonstrates that F. equiseti can be transmitted by Diplotaxis tenuifolia seeds. The external nature of seed contamination of Diplotaxis sp. by F. equiseti has been proved. More extensive essays on seed lots of cultivated rocket are needed in order to have a better understanding of the seed transmissibility of the pathogen on such a host.

  • first report of leaf spot of wild rocket Diplotaxis tenuifolia caused by myrothecium verrucaria in italy
    Journal of Plant Pathology, 2016
    Co-Authors: Angelo Garibaldi, S Francoortega, Giovanna Gilardi, Maria Lodovica Gullino
    Abstract:

    During the spring of 2015, plants of wild rocket (Diplotaxis tenuifolia) cv. Frastagliata grown under tunnel (1 ha) in the Campania region near Salerno (Southern Italy) showed symptoms of a previously unknown foliar disease. First symptoms developed at temperatures of 16 to 18°C on 14- day-old plants and consisted of small circular, sunken grey brown spots (1 mm in diameter) with a well-defined border. As the lesions expanded (up to 30 mm in diameter) they developed concentric rings, coalesced, and the affected tissues became water-soaked. Lesions appeared on both foliar sides on 4 to 7% of plants. Roots were asymptomatic. Isolations were carried out from symptomatic leaf tissues on potato dextrose agar (PDA) amended with 25 mg/l of streptomycin sulphate. A white fungus, that developed black sporodochia 7 to 10 days after plating, was obtained. Cylindrical, non- septate conidia, measuring 4.4 to 9.5 (6.9) μm and 1.1 to 2.9 (1.9) μm, showed the morphological characteristics of Myrothecium sp. (Domsch et al., 1980). The Internal Transcribed Spacer (ITS) region of rDNA was amplified using the primers ITS1/ITS4. The 510 bp product was sequenced (GenBank accession No. KX298062) and showed a 100% similarity with Myrothecium verrucaria (KT354922). Pathogenicity tests were carried out in a growth chamber (20-24°C and 12 h photoperiod), on 15-day-old wild rocket plants cv. Frastagliata transplanted in 2-liter pots. Fifteen plants per pot (5 pots/treatment) were sprayed with 1x106 conidia/ml of one representative isolate of the pathogen or with distilled water, covered with transparent plastic bags for 5 days. Spots similar to those observed on the original plants developed 5 days after inoculation. All the non-inoculated plants remained asymptomatic. M. verrucaria was consistently isolated from all the symptomatic plants. The pathogenicity test was carried out twice, showing the same results. Though M. verrucaria has a wide host range (Farr and Rossman, 2016), this is the first report on D. tenuifolia in Italy as well as worldwide.

  • real time pcr for rapid in planta detection of plectosphaerella cucumerina on wild rocket Diplotaxis tenuifolia
    Phytopathologia Mediterranea, 2016
    Co-Authors: Giovanna Gilardi, Maria Lodovica Gullino, Kathryn Webb, G Ortu, Angelo Garibaldi
    Abstract:

    Plectosphaerella cucumerina that has recently been described as causing a leaf spot on wild rocket ( Diplotaxis tenuifolia ) in Italy, but has not yet been recorded on this host in other countries. This fungus has the potential to cause severe economic losses to the salad-producing industry, especially in Italy. This paper describes the development of a real-time PCR assay based on internal transcribed spacer (ITS) sequence, to aid diagnosis of this pathogen in culture and in plant material. The assay showed no cross reaction with closely related Plectosphaerella species or with 18 other commonly found fungi, and was able to detect 10 fg of Pa. cucumerina DNA per reaction. The assay was more specific than previously available molecular methods. A comparison of molecular and isolation methods indicated that the real-time PCR method is suitable for detection of the pathogen on plant material.

  • seed transmission of plectosphaerella cucumerina causal agent of leaf spot of Diplotaxis tenuifolia in italy
    Phytoparasitica, 2013
    Co-Authors: Giovanna Gilardi, Angelo Garibaldi, Maria Lodovica Gullino
    Abstract:

    Plectosphaerella cucumerina has been described recently as the causal agent of a leaf spot on wild rocket (Diplotaxis tenuifolia). Eight seed samples of wild rocket obtained from commercial seed lots used for sowing by farms severely affected by P. cucumerina, were assayed for the presence of the pathogen. Isolations were carried out on subsamples of seeds (400) unwashed or disinfected in 1% sodium hypochloride. The pathogenicity of the isolates of P. cucumerina obtained was tested in two trials carried out on wild rocket; four out of eight samples of rocket seeds were contaminated by P. cucumerina. Eleven isolates of P. cucumerina were obtained from 7,200 not disinfected seeds tested, while none was isolated from an equal number of disinfected seeds. All isolates were pathogenic on wild rocket. The results obtained indicate that rocket seeds are a potential source of inoculum for P. cucumerina. The possibility of isolating the pathogen from seeds, albeit from a low percentage of them, supports the hypothesis that the rapid spread of this new disease of rocket recently observed in Italy is due to the use of infected propagation material. Measures for prevention and control of the disease are discussed.

  • first report of plectosphaerella cucumerina on greenhouse cultured wild rocket Diplotaxis tenuifolia in italy
    Plant Disease, 2012
    Co-Authors: A Garibaldi, Giovanna Gilardi, G Ortu, Maria Lodovica Gullino
    Abstract:

    During spring 2012, symptoms of an unusual leaf spot disease were observed in several commercial greenhouses near Salerno (southern Italy) on plants of Diplotaxis tenuifolia (cv Selvatica). The first symptoms on leaves of affected plants consisted of small (1 mm) black-brown spots of irregular shape, later coalescing into larger spots, 1 cm in diameter. Spots were surrounded by a yellow halo, and were mostly located on the foliar limb, rib, and petiole. Affected leaves were often distorted, appearing hook-like. The disease was severe under 75 to 90% RH, at air temperature of 20 to 26°C, and caused severe production losses on about 50 ha. Particularly, affected tissues rotted quickly after packaging and during transit and commercialization of processed rocket. Diseased tissue was excised, immersed in a solution containing 1% sodium hypochlorite for 60 s, rinsed in water, then placed on potato dextrose agar (PDA) medium, containing 25 mg/l of streptomycin sulphate. After 5 days, a fungus developed producing a whitish-orange mycelium when incubated under 12 h/day of fluorescent light at 22°C. The isolates obtained were purified on PDA. On this medium, they produced hyaline elliptical and ovoid conidia, sometimes one-septate, measuring 4.5 to 9.2 × 1.7 to 3.5 (average 6.8 × 2.6) μm. Conidia were born on phialides, measuring 6.8 to 20.2 × 1.3 to 3.1 (average 16.5 × 2.1) μm. Such characteristics are typical of Plectosphaerella sp. (2). The internal transcribed spacer (ITS) region of rDNA was amplified using the primers ITS1/ITS4 (3) and sequenced. BLAST analysis of the 519-bp segment showed a 98% similarity with the sequence of Plectosphaerella cucumerina (GenBank Accession No. AB469880). The nucleotide sequence has been assigned the GenBank Accession JX185769. To confirm pathogenicity, tests were conducted on 45-day-old D. tenuifolia plants. Plants (21/treatment), grown in 15 liter pots (7 plants/pot) were inoculated by spraying a 1 × 106 CFU/ml conidial suspension of one isolate of P. cucumerina, prepared from 10-day-old cultures, grown on PDA. Inoculated plants were maintained in a growth chamber at 23 ± 1°C, at 90% RH for 4 days. Non-inoculated plants served as control. Inoculated plants showed the typical first leaf spots 6 days after the artificial inoculation. Four days later, spots enlarged and leaves became distorted, showing chlorosis. Non-inoculated plants remained healthy. P. cucumerina was reisolated from inoculated plants. The pathogenicity test was conducted twice with identical results. This is, to our knowledge, the first report of P. cucumerina on D. tenuifolia in Italy as well as worldwide. P. cucumerina has been described as associated with root and collar rots of other horticultural crops in southern Italy (1). Due to the importance of the crop in Italy, this disease can cause serious economic losses. References: (1) A. Carlucci et al., Persoonia, 28:34, 2012. (2) M. E. Palm et al. Mycologia, 87:397, 1995. (3) T. J. White et al. PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, San Diego, 1990.

Hilary J Rogers - One of the best experts on this subject based on the ideXlab platform.

  • gene expression analysis of rocket salad under pre harvest and postharvest stresses a transcriptomic resource for Diplotaxis tenuifolia
    PLOS ONE, 2017
    Co-Authors: Marina Cavaiuolo, Natasha D Spadafora, Carsten Theodor Muller, Hilary J Rogers, G Cocetta, A Ferrante
    Abstract:

    Diplotaxis tenuifolia L. is of important economic value in the fresh-cut industry for its nutraceutical and sensorial properties. However, information on the molecular mechanisms conferring tolerance of harvested leaves to pre- and postharvest stresses during processing and shelf-life have never been investigated. Here, we provide the first transcriptomic resource of rocket by de novo RNA sequencing assembly, functional annotation and stress-induced expression analysis of 33874 transcripts. Transcriptomic changes in leaves subjected to commercially-relevant pre-harvest (salinity, heat and nitrogen starvation) and postharvest stresses (cold, dehydration, dark, wounding) known to affect quality and shelf-life were analysed 24h after stress treatment, a timing relevant to subsequent processing of salad leaves. Transcription factors and genes involved in plant growth regulator signaling, autophagy, senescence and glucosinolate metabolism were the most affected by the stresses. Hundreds of genes with unknown function but uniquely expressed under stress were identified, providing candidates to investigate stress responses in rocket. Dehydration and wounding had the greatest effect on the transcriptome and different stresses elicited changes in the expression of genes related to overlapping groups of hormones. These data will allow development of approaches targeted at improving stress tolerance, quality and shelf-life of rocket with direct applications in the fresh-cut industries.

  • multi trait analysis of post harvest storage in rocket salad Diplotaxis tenuifolia links sensorial volatile and nutritional data
    Food Chemistry, 2016
    Co-Authors: Natasha D Spadafora, A L Amaro, Maria Joao Pereira, Carsten Theodor Muller, Manuela Pintado, Hilary J Rogers
    Abstract:

    Rocket salad (Diplotaxis tenuifolia; wild rocket) is an important component of ready to eat salads providing a distinct peppery flavour and containing nutritionally relevant compounds. Quality deteriorates during post-harvest, in relation to time and storage temperature amongst other factors. Volatile organic compounds (VOCs) are easily measurable from rocket leaves and may provide useful quality indicators for e.g. changes in isothiocyanates derived from nutritionally important glucosinolates. VOC profiles discriminated storage temperatures (0, 5 and 10°C) and times (over 14days). More specifically, concentrations of aldehydes and isothiocyanates decreased with time paralleling a fall in vitamin C and a reduction in sensorial quality at the two higher temperatures. Sulphur containing compounds rise at later time-points and at higher temperatures coincident with an increase in microbial titre, mirroring a further drop in sensorial quality thus indicating their contribution to off-odours.

Verloove F. - One of the best experts on this subject based on the ideXlab platform.

  • Nuevos xenófitos de La Palma (Islas Canarias, España), con énfasis en las especies naturalizadas y (potencialmente) invasoras. Parte 3
    'Editorial CSIC', 2020
    Co-Authors: Otto R., Verloove F.
    Abstract:

    Several months of field work in La Palma (western Canary Islands) yielded a number of interesting new records of non-native vascular plants. Alstroemeria aurea, A. ligtu, Anacyclus radiatus subsp. radiatus, Chenopodium album subsp. borbasii, Cotyledon orbiculata, Cucurbita ficifolia, Cynodon nlemfuensis, Datura stramonium subsp. Tatula, Digitaria ciliaris var. rhachiseta, D. ischaemum, Diplotaxis tenuifolia, Egeria densa, Eugenia uniflora, Galinsoga quadriradiata, Glebionis segetum, Kalanchoe laetivirens, Lemna minuta, Ligustrum lucidum, Lotus broussonetii, Oenothera fallax, Paspalum notatum, Passiflora caerulea, P. manicata × tarminiana, P. tarminiana, Pelargonium capitatum, Phaseolus lunatus, Portulaca trituberculata, Pyracantha angustifolia, Sedum mexicanum, Trifolium lappaceum, Urochloa mutica, U. subquadripara and Volutaria tubuliflora are naturalized or (potentially) invasive xenophytes or of special floristic interest, reported for the first time from either the Canary Islands or La Palma. Three additional, presumably ephemeral taxa are reported for the first time from the Canary Islands, whereas seven ephemeral taxa are new for La Palma.Varios meses de trabajos de campo en La Palma (Islas Canarias occidentales) han posibilitado el descubrimiento de nuevas plantas vasculares no nativas. Alstroemeria aurea, A. ligtu, Anacyclus radiatus subsp. Radiatus, Chenopodium album subsp. borbasii, Cotyledon orbiculata, Cucurbita ficifolia, Cynodon nlemfuensis, Datura stramonium subsp. tatula, Digitaria ciliaris var. rhachiseta, D. ischaemum, Diplotaxis tenuifolia, Egeria densa, Eugenia uniflora, Galinsoga quadriradiata, Glebionis segetum, Kalanchoe laetivirens, Lemna minuta, Ligustrum lucidum, Lotus broussonetii, Oenothera fallax, Paspalum notatum, Passiflora caerulea, P. manicata × tarminiana, P. tarminiana, Pelargonium capitatum, Phaseolus lunatus, Portulaca trituberculata, Pyracantha angustifolia, Sedum mexicanum, Trifolium lappaceum, Urochloa mutica, U. subquadripara y Volutaria tubuliflora son xenófitos naturalizados o (potencialmente) invasores o de especial interés florístico, que se citan por primera vez para las Islas Canarias o para la isla de La Palma. Tres táxones adicionales, probablemente casuales, se dan a conocer por primera vez de las Islas Canarias, y siete táxones de la isla de La Palma

  • Nuevos xenófitos de Gran Canaria (Islas Canarias, España), con énfasis en las especies naturalizadas y (potencialmente) invasoras
    'Editorial CSIC', 2013
    Co-Authors: Verloove F.
    Abstract:

    Trabajos recientes de campo en Gran Canaria han facilitado el descubrimiento de nuevas localidades para plantas vasculares no nativas. Agave attenuata, Antigonon leptopus, Atriplex nummularia, Cascabela thevetia, Cenchrus echinatus, Cuscuta campestris, Diplachne fusca subsp. uninervia, Diplotaxis tenuifolia, Dysphania anthelmintica (hasta ahora confundida con D. ambrosioides), Eclipta prostrata, Euphorbia pulcherrima, Fagopyrum esculentum, Gossypium barbadense, Lablab purpureus, Lemna minuta, Opuntia leucotricha, Passiflora edulis, Pennisetum glaucum, Phaseolus acutifolius, Pluchea carolinensis, Prosopis juliflora, Salvia microphylla, Schinus terebinthifolius, Senna spectabilis, Solanum chrysotrichum, Tecoma stans, Tipuana tipu, Urochloa mutica, U. plantaginea y Washingtonia se citan por primera vez para las Islas Canarias, mientras que Alopecurus myosuroides, Amaranthus blitoides, Bothriochloa ischaemum var. songarica, Cardamine flexuosa subsp. debilis, Heliotropium curassavicum, Leonotis nepetifolia, Medicago lupulina, Parkinsonia aculeata, Physalis peruviana, Phytolacca americana y Turnera ulmifolia son nuevas para la flora de la isla de Gran Canaria. Finalmente, se confirma la presencia de Paspalum vaginatum, P. distichum y Cortaderia selloana en Gran Canaria.Trabajos recientes de campo en Gran Canaria han facilitado el descubrimiento de nuevas localidades para plantas vasculares no nativas. Agave attenuata, Antigonon leptopus, Atriplex nummularia, Cascabela thevetia, Cenchrus echinatus, Cuscuta campestris, Diplachne fusca subsp. uninervia, Diplotaxis tenuifolia, Dysphania anthelmintica (hasta ahora confundida con D. ambrosioides), Eclipta prostrata, Euphorbia pulcherrima, Fagopyrum esculentum, Gossypium barbadense, Lablab purpureus, Lemna minuta, Opuntia leucotricha, Passiflora edulis, Pennisetum glaucum, Phaseolus acutifolius, Pluchea carolinensis, Prosopis juliflora, Salvia microphylla, Schinus terebinthifolius, Senna spectabilis, Solanum chrysotrichum, Tecoma stans, Tipuana tipu, Urochloa mutica, U. plantaginea y Washingtonia se citan por primera vez para las Islas Canarias, mientras que Alopecurus myosuroides, Amaranthus blitoides, Bothriochloa ischaemum var. songarica, Cardamine flexuosa subsp. debilis, Heliotropium curassavicum, Leonotis nepetifolia, Medicago lupulina, Parkinsonia aculeata, Physalis peruviana, Phytolacca americana y Turnera ulmifolia son nuevas para la flora de la isla de Gran Canaria. Finalmente, se confirma la presencia de Paspalum vaginatum, P. distichum y Cortaderia selloana en Gran Canaria

  • New xenophytes from Gran Canaria (Canary Islands, Spain), with emphasis on naturalized and (potentially) invasive species
    Consejo Superior de Investigaciones Científicas, 2013
    Co-Authors: Verloove F.
    Abstract:

    Trabajos recientes de campo en Gran Canaria han facilitado el descubrimiento de nuevas localidades para plantas vasculares no nativas. Agave attenuata, Antigonon leptopus, Atriplex nummularia, Cascabela thevetia, Cenchrus echinatus, Cuscuta campestris, Diplachne fusca subsp. uninervia, Diplotaxis tenuifolia, Dysphania anthelmintica (hasta ahora confundida con D. ambrosioides), Eclipta prostrata, Euphorbia pulcherrima, Fagopyrum esculentum, Gossypium barbadense, Lablab purpureus, Lemna minuta, Opuntia leucotricha, Passiflora edulis, Pennisetum glaucum, Phaseolus acutifolius, Pluchea carolinensis, Prosopis juliflora, Salvia microphylla, Schinus terebinthifolius, Senna spectabilis, Solanum chrysotrichum, Tecoma stans, Tipuana tipu, Urochloa mutica, U. plantaginea y Washingtonia se citan por primera vez para las Islas Canarias, mientras que Alopecurus myosuroides, Amaranthus blitoides, Bothriochloa ischaemum var. songarica, Cardamine flexuosa subsp. debilis, Heliotropium curassavicum, Leonotis nepetifolia, Medicago lupulina, Parkinsonia aculeata, Physalis peruviana, Phytolacca americana y Turnera ulmifolia son nuevas para la flora de la isla de Gran Canaria. Finalmente, se confirma la presencia de Paspalum vaginatum, P. distichum y Cortaderia selloana en Gran Canaria.Trabajos recientes de campo en Gran Canaria han facilitado el descubrimiento de nuevas localidades para plantas vasculares no nativas. Agave attenuata, Antigonon leptopus, Atriplex nummularia, Cascabela thevetia, Cenchrus echinatus, Cuscuta campestris, Diplachne fusca subsp. uninervia, Diplotaxis tenuifolia, Dysphania anthelmintica (hasta ahora confundida con D. ambrosioides), Eclipta prostrata, Euphorbia pulcherrima, Fagopyrum esculentum, Gossypium barbadense, Lablab purpureus, Lemna minuta, Opuntia leucotricha, Passiflora edulis, Pennisetum glaucum, Phaseolus acutifolius, Pluchea carolinensis, Prosopis juliflora, Salvia microphylla, Schinus terebinthifolius, Senna spectabilis, Solanum chrysotrichum, Tecoma stans, Tipuana tipu, Urochloa mutica, U. plantaginea y Washingtonia se citan por primera vez para las Islas Canarias, mientras que Alopecurus myosuroides, Amaranthus blitoides, Bothriochloa ischaemum var. songarica, Cardamine flexuosa subsp. debilis, Heliotropium curassavicum, Leonotis nepetifolia, Medicago lupulina, Parkinsonia aculeata, Physalis peruviana, Phytolacca americana y Turnera ulmifolia son nuevas para la flora de la isla de Gran Canaria. Finalmente, se confirma la presencia de Paspalum vaginatum, P. distichum y Cortaderia selloana en Gran Canaria

Fernández Acevedo Victoria - One of the best experts on this subject based on the ideXlab platform.

  • Estudio de especies hortícolas aptas para su uso en la biofumigación
    2016
    Co-Authors: Fernández Acevedo Victoria
    Abstract:

    La biofumigación es un método de remplazo a las fumigaciones químicas de suelos. Se basa en la acción de sustancias volátiles producidas por la degradación de la materia orgánica para controlar los agentes patógenos de suelo. Las especies más utilizadas son las Brassicaceae, por su alta concentración de glucosinolatos. En este trabajo se analizaron las concentraciones de glucosinolatos en mostaza blanca (S. alba), brócoli (B. oleracea var. Itálica) y rúcula selvática(Diplotaxis tenuifolia) con un método indirecto, el cual presenta fácil realización y acceso. Además, se realizó un ensayo bajo invernadero con dos variedades de brócoli para analizar el rastrojo que queda en suelo.Área: Ciencias Agrícolas, Produccion y Salud Anima

  • Estudio de especies hortícolas aptas para su uso en la biofumigación
    2016
    Co-Authors: Fernández Acevedo Victoria
    Abstract:

    La biofumigación es un método de remplazo a las fumigaciones químicas de suelos. Se basa en la acción de sustancias volátiles producidas por la degradación de la materia orgánica para controlar los agentes patógenos de suelo. Las especies más utilizadas son las Brassicaceae, por su alta concentración de glucosinolatos. En este trabajo se analizaron las concentraciones de glucosinolatos en mostaza blanca (S. alba), brócoli (B. oleracea var. Itálica) y rúcula selvática (Diplotaxis tenuifolia) con un método indirecto, el cual presenta fácil realización y acceso. Además, se realizó un ensayo bajo invernadero con dos variedades de brócoli para analizar el rastrojo que queda en suelo.Área: Ciencias Agrícolas, Produccion y Salud AnimalFacultad de Ciencias Agrarias y Forestale

Natasha D Spadafora - One of the best experts on this subject based on the ideXlab platform.

  • gene expression analysis of rocket salad under pre harvest and postharvest stresses a transcriptomic resource for Diplotaxis tenuifolia
    PLOS ONE, 2017
    Co-Authors: Marina Cavaiuolo, Natasha D Spadafora, Carsten Theodor Muller, Hilary J Rogers, G Cocetta, A Ferrante
    Abstract:

    Diplotaxis tenuifolia L. is of important economic value in the fresh-cut industry for its nutraceutical and sensorial properties. However, information on the molecular mechanisms conferring tolerance of harvested leaves to pre- and postharvest stresses during processing and shelf-life have never been investigated. Here, we provide the first transcriptomic resource of rocket by de novo RNA sequencing assembly, functional annotation and stress-induced expression analysis of 33874 transcripts. Transcriptomic changes in leaves subjected to commercially-relevant pre-harvest (salinity, heat and nitrogen starvation) and postharvest stresses (cold, dehydration, dark, wounding) known to affect quality and shelf-life were analysed 24h after stress treatment, a timing relevant to subsequent processing of salad leaves. Transcription factors and genes involved in plant growth regulator signaling, autophagy, senescence and glucosinolate metabolism were the most affected by the stresses. Hundreds of genes with unknown function but uniquely expressed under stress were identified, providing candidates to investigate stress responses in rocket. Dehydration and wounding had the greatest effect on the transcriptome and different stresses elicited changes in the expression of genes related to overlapping groups of hormones. These data will allow development of approaches targeted at improving stress tolerance, quality and shelf-life of rocket with direct applications in the fresh-cut industries.

  • multi trait analysis of post harvest storage in rocket salad Diplotaxis tenuifolia links sensorial volatile and nutritional data
    Food Chemistry, 2016
    Co-Authors: Natasha D Spadafora, A L Amaro, Maria Joao Pereira, Carsten Theodor Muller, Manuela Pintado, Hilary J Rogers
    Abstract:

    Rocket salad (Diplotaxis tenuifolia; wild rocket) is an important component of ready to eat salads providing a distinct peppery flavour and containing nutritionally relevant compounds. Quality deteriorates during post-harvest, in relation to time and storage temperature amongst other factors. Volatile organic compounds (VOCs) are easily measurable from rocket leaves and may provide useful quality indicators for e.g. changes in isothiocyanates derived from nutritionally important glucosinolates. VOC profiles discriminated storage temperatures (0, 5 and 10°C) and times (over 14days). More specifically, concentrations of aldehydes and isothiocyanates decreased with time paralleling a fall in vitamin C and a reduction in sensorial quality at the two higher temperatures. Sulphur containing compounds rise at later time-points and at higher temperatures coincident with an increase in microbial titre, mirroring a further drop in sensorial quality thus indicating their contribution to off-odours.