The Experts below are selected from a list of 459 Experts worldwide ranked by ideXlab platform

Manuel Bernabé - One of the best experts on this subject based on the ideXlab platform.

Oussama Ahrazem - One of the best experts on this subject based on the ideXlab platform.

Jozef Nosek - One of the best experts on this subject based on the ideXlab platform.

  • Isolation of genes coding for Ade2 and Ura3 homologues from the multinuclear yeast Dipodascus magnusii.
    Current genetics, 2002
    Co-Authors: Martin Kucej, Peter Kosa, L'ubica Adamíková, Jozef Nosek
    Abstract:

    Dipodascus magnusii is a nonconventional yeast species with giant multinuclear cells. We constructed two genomic DNA libraries in plasmid vectors and isolated the first two D. magnusii protein-encoding genes, DmADE2 and DmURA3, coding for phosphoribosylaminoimidazole carboxylase and orotidine-5′-phosphate decarboxylase, respectively. Both genes represent functional orthologues, since they complement ade2 and ura3 mutations in Saccharomyces cerevisiae and their putative products possess conserved sequences important for enzymatic activities. Moreover, the results of Southern blot analysis indicate that the genome of D. magnusii contains additional, paralogous sequences of the DmADE2 and DmURA3 genes.

  • Development of a transformation system for the multinuclear yeast Dipodascus (Endomyces) magnusii.
    Yeast (Chichester England), 1998
    Co-Authors: L'ubica Adamíková, Peter Griač, Lubomir Tomaska, Jozef Nosek
    Abstract:

    We have developed the first system for genetic transformation of the multinuclear yeast Dipodascus magnusii. The system is based on a dominant selectable marker and an autonomously replicating sequence. We have constructed a plasmid vector which contains a marker conferring resistance to zeocin and the segment of non-transcribed spacer of D. magnusii ribosomal DNA which supports the autonomous replication of plasmid DNA in yeast cells. Plasmid DNA has been transferred into D. magnusii cells by electroporation. The DNA sequence which is described in this article has been deposited in the EMBL data library under Accession Number Y14587. © 1998 John Wiley & Sons, Ltd.

  • Isolation of a dsRNA virus from Dipodascus (Endomyces) magnusii
    Current Genetics, 1993
    Co-Authors: Jozef Nosek, Dominik Filipp, Klára Bederkova, Peter Griač
    Abstract:

    Virus-like particles (VLPs) of 40 nm diameter were isolated from the yeast-like fungus Dipodascus magnusii . These VLPs copurify with several linear double-stranded RNA molecules of different size. We have found some polymorphism in both the length and the number of these dsRNAs among six D. magnusii strains. Analysis of CsCl gradient-purified VLPs on PAGE/SDS electrophoresis showed one major protein component with an apparent molecular weight of 75 kDa.

Renata Zvyagilskaya - One of the best experts on this subject based on the ideXlab platform.

  • Effect of prooxidants on yeast mitochondria
    Journal of Bioenergetics and Biomembranes, 2011
    Co-Authors: Tat’yana Trendeleva, Evgeniya Sukhanova, Ludmila Ural’skaya, Nils-erik Saris, Renata Zvyagilskaya
    Abstract:

    Tightly coupled mitochondria from Yarrowia lipolytica and Dipodascus ( Endomyces ) magnusii yeasts were used in this study. The two yeasts are aerobes containing the fully competent respiratory chain with three energy conservation sites. Interaction of the yeast mitochondria with prooxidants (diamide, menadione, oxaloacetate, phenylarsine oxide, hydrogen peroxide, t -butyl peroxide, and ascorbate plus Fe^2+) was studied. The prooxidants, depending on their chemical nature, either caused uncoupling (e.g., activated state 4 respiration) or inhibited oxidation of respiratory substrates. All of the agents dissipated the membrane potential without megachannel formation (no large-scale swelling of mitochondria was observed). Except for combined application of ascorbate and Fe^2+, the prooxidant-induced decrease in the membrane potential was specifically prevented by ATP, even in the cases when classic antioxidants, e.g., N-acetylcysteine, were ineffective. No permeabilization of yeast mitochondria was observed under concerted action of prooxidants and Ca^2+, suggesting that an mPTP-like pore, if it ever occurs in yeast mitochondria, is not coupled with Ca^2+ uptake.

Eric Record - One of the best experts on this subject based on the ideXlab platform.

  • Fungal diversity in anoxic-sulfidic sediments in a mangrove soil
    Fungal Ecology, 2012
    Co-Authors: Yonathan Arfi, Cyril Marchand, Morgane Wartel, Eric Record
    Abstract:

    Fungi are important actors in the ecological processes occurring in mangroves and are thought to play a role in organic matter decomposition pathways, despite the fact that a large fraction of the carbon processing in mangroves happens in the anoxic bulk sediment. The fungal diversity in these strata is, however, poorly known. Here, using microscopic observations of calcofluor white stained samples, we detected the presence of fungal hyphae indicating that fungal growth was occurring under these anoxic and sulfidic conditions. To assess the fungal diversity we used 454 pyrosequencing of the nuclear ribosomal internal transcribed spacer land 2 (ITS-1 and ITS-2). We analysed approximately 5 000 reads for each ITS, corresponding to around 115 molecular operational taxonomic units. The taxonomic analyses identified the Agaricomycetes as the dominant fungal class. The most abundant operational taxonomic units were affiliated to: a taxon with affinity to the genus Sistotremastrum (Trechisporales), Dipodascus australiensis (Saccharomycetales), an Alternaria species (Pleosporales) and an unknown Lecanoromycete. This study indicates the presence of rich fungal communities in anoxic mangroves sediments. It also raises the question of the adaptation of these organisms to the environmental conditions and of the roles they play in organic matter decomposition processes. (C) 2011 Elsevier Ltd and The British Mycological Society. All rights reserved.