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Contreras Aguilar, Giuliana Giaminna - One of the best experts on this subject based on the ideXlab platform.

  • Prevalencia de toxocara canis en caninos domesticos del distrito de pataz, región la libertad, peru, enero -marzo 2016
    'Universidad Privada Antenor Orrego', 2017
    Co-Authors: Contreras Aguilar, Giuliana Giaminna
    Abstract:

    In order to determine the prevalence of toxocariasis in domestic canines of Pataz’s district, La Libertad region, 164 fecal samples were processed through two methods, direct and concentration by flotation. The proportion of the population with parasitosis was 82,32% (135/164), corresponding to Toxocara canis 14.07% (19/135), Dipylidium caninum 5.2% (7/135) and Ancylostoma spp. 80.74% (109 / 135) of prevalence respectively.Con el propósito de determinar la prevalencia de toxocariasis en caninos domésticos del distrito de Pataz, región La Libertad, se procesaron 164 muestras fecales mediante los métodos directo y concentración por flotación. La proporción de la población con parasitosis fue de 82,32% (135/164), correspondiendo a Toxocara canis 14,07% (19/135), Dipylidium caninum 5.2% (7/135) y Ancylostoma spp. 80.74% (109/135) de prevalencia respectivamente

  • Prevalencia de Toxocara canis en caninos domésticos del distrito de Pataz, región La Libertad, Perú, enero -marzo 2016
    Universidad Privada Antenor Orrego - UPAO, 2017
    Co-Authors: Contreras Aguilar, Giuliana Giaminna
    Abstract:

    Con el propósito de determinar la prevalencia de toxocariasis en caninos domésticos del distrito de Pataz, región La Libertad, se procesaron 164 muestras fecales mediante los métodos directo y concentración por flotación. La proporción de la población con parasitosis fue de 82,32% (135/164), correspondiendo a Toxocara canis 14,07% (19/135), Dipylidium caninum 5.2% (7/135) y Ancylostoma spp. 80.74% (109/135) de prevalencia respectivamente.In order to determine the prevalence of toxocariasis in domestic canines of Pataz’s district, La Libertad region, 164 fecal samples were processed through two methods, direct and concentration by flotation. The proportion of the population with parasitosis was 82,32% (135/164), corresponding to Toxocara canis 14.07% (19/135), Dipylidium caninum 5.2% (7/135) and Ancylostoma spp. 80.74% (109 / 135) of prevalence respectively.Tesi

Josephus J. Fourie - One of the best experts on this subject based on the ideXlab platform.

  • analysis of Dipylidium caninum tapeworms from dogs and cats or their respective fleas part 2 distinct canine and feline host association with two different Dipylidium caninum genotypes
    Parasite, 2018
    Co-Authors: Frederic Beugnet, Michel Labuschagne, Dionne Crafford, Josephus J. Fourie
    Abstract:

    Initial investigations suggested the existence of two distinct genotypes of Dipylidium caninum from infected cat fleas ( Ctenocephalides felis ). One genotype was found almost always (> 95%) in fleas collected from, and proglottids shed by, domestic dogs. The other was found almost always (> 95%) in fleas collected from, and proglottids shed by, domestic cats. Molecular investigations (Part 1, in this journal) confirmed the presence of two distinct genotypes. Due to the apparent host association observed, these were referred to as the “ D. caninum canine genotype” and the “ D. caninum feline genotype”. The current article reports on an in vivo experimental infection study assessing the host-parasite interaction for each genotype. Mixed infections with the two genotypes in both dogs and cats were conducted. The specific genotyping of proglottids allowed us to assess the specific prepatent periods, prolificity, and longevity of each genotype in dogs versus cats. The possible hybridisation was also studied through molecular evaluation of the proglottids expelled by infected dogs and cats. Results demonstrate a clear distinct host interaction. The canine D. caninum genotype occurred at a higher frequency in dogs, with a shorter prepatent period and a longer lifespan; and the feline genotype occurred at a higher frequency in cats, with a shorter prepatent period and a longer lifespan. The absence of any hybrids in the mixed infections of both dogs and cats confirm the hypothesis of two distinct genotypes, suggesting the possibility of two distinct species within Dipylidium caninum .

  • analysis of Dipylidium caninum tapeworms from dogs and cats or their respective fleas part 1 molecular characterization of Dipylidium caninum genetic analysis supporting two distinct species adapted to dogs and cats
    Parasite, 2018
    Co-Authors: Michel Labuschagne, Frederic Beugnet, Josephus J. Fourie, Steffen Rehbein, Jacques Guillot, Dionne Crafford
    Abstract:

    A 28S rDNA PCR detection assay was previously developed to identify Dipylidium caninum DNA inside single fleas collected from both cats and dogs. Sequence analysis of the 28S rDNA fragment indicated two genetically distinct variations of the target region. The two genotypes, so-called “D.  caninum canine genotype” and “D.  caninum feline genotype”, based on host origin, are further investigated and described in this paper. Restriction fragment length polymorphism (RFLP) analysis and hydrolysis probe-based genotyping assays were developed and validated for genotyping D.  caninum DNA. The complete mitochondrial (mt) genome of the “feline genotype” was sequenced and compared to the D.  caninum mt genome available in GenBank. The molecular characterization of D.  caninum isolates collected from infected fleas, and also proglottids collected from dogs and cats, confirmed the existence of two distinct genotypes. These genotypes are related to host origin (dogs or cats), irrespective of their geographical origin, and they present a biological adaptation to their respective host, as confirmed by the comparison of biological development and host preference in another study. The genetic differences (Part 1, present paper) and biological observations (Part 2, in this journal) enabled us to suggest the existence of two distinct species within D.  caninum , which will have to be clarified.

  • preventive efficacy of nexgard spectra against Dipylidium caninum infection in dogs using a natural flea ctenocephalides felis infestation model
    Parasite, 2017
    Co-Authors: Frederic Beugnet, Josephus J. Fourie, Leon Meyer, Diane Larsen
    Abstract:

    The efficacy of a monthly oral endectocide product, NexGard Spectra® (Merial), a combination of afoxolaner and milbemycin oxime, was evaluated in a flea (Ctenocephalides felis ) challenge model for the prevention of Dipylidium caninum tapeworm infection in dogs. The efficacy of treatment with NexGard Spectra® was assessed in 10 dogs following weekly flea infestation with metacestode naturally infected fleas and compared with that in 10 untreated control dogs. The 100 fleas deposited weekly on each dog were not removed until Day 35, allowing enough time for their ingestion. The microscopical analysis of 30 fleas from the flea batches before each weekly challenge demonstrated that 10–33% of the fleas were infected by D. caninum cysticercoid larvae. The arithmetic mean flea count recorded was 47.7 for the 10 untreated dogs and 0 for the 10 treated dogs at Day 35. Based on the daily collection of expelled D. caninum proglottids by dogs during the 70 days of the study, 70% (7/10) of the control dogs and 0% (0/10) of the treated dogs were infected with D. caninum (p treatment provided indirect prevention of D. caninum infestation. No treatment-related adverse events were observed in dogs during this study.

  • Occurrence of Dipylidium caninum in fleas from client-owned cats and dogs in Europe using a new PCR detection assay
    Veterinary Parasitology, 2014
    Co-Authors: Frederic Beugnet, Michel Labuschagne, Josephus J. Fourie, Guillot Jacques, Róbert Farkas, Vasile Cozma, Lénaïg Halos, Klaus Hellmann, Martin Knaus, Steffen Rehbein
    Abstract:

    Ctenocephalides fleas are not only the most prevalent ectoparasites of dogs and cats but also the intermediate host of the cestode Dipylidium caninum. Due to the poor sensitivity of coproscopy to diagnose cat and dog infestation by Dipylidium, few epidemiological data are available on its prevalence among pet populations. A new PCR method was developed to specifically identify D. caninum rDNA inside single fleas. The PCR test was then applied to 5529 fleas of Ctenocephalides genus, 2701 Ctenocephalides felis fleas (1969 collected on 435 cats and 732 on 178 dogs) and 2828 Ctenocephalides canis fleas collected from 396 dogs. Precisely, 4.37% of cats were infested by a flea population infected with D. caninum. Out of the 1969 C. felis from cats, 2.23% were found to be infected with Dipylidium. From the 396 dogs infested with C. canis, 9.1%% were infested with the Dipylidium infected fleas, which is significantly higher than the observation made in cats (p=0.03). Moreover, 3.1% of the C. canis fleas were found to be infected with Dipylidium, which is not significantly different than in C. felis. Looking at the number of infected fleas in the positive samples (at least one PCR positive flea in a sample), the infestation rate in samples was varied from 3 to 100% with an average of 19.7% which is in favour of easy and regular Dipylidium reinfestations of both cats and dogs in households. For the first time, the spread of D. caninum between fleas and dogs and cats is confirmed throughout Europe.

  • prophylactic treatment of flea infested dogs with an imidacloprid flumethrin collar seresto bayer to preempt infection with Dipylidium caninum
    Parasitology Research, 2013
    Co-Authors: Josephus J. Fourie, Dionne Crafford, Ivan Gerard Horak, Dorothee Stanneck
    Abstract:

    : The objective of the study was to determine the sustained effectiveness of 10 % imidacloprid and 4.5 % flumethrin, incorporated in a slow-release matrix collar, in preventing Dipylidium caninum infection in dogs after repeated laboratory infestations with fleas infected with metacestodes of this tapeworm. Efficacy against infection with D. caninum was evaluated by infesting 16 dogs with cat fleas (Ctenocephalides felis) on study days 7, 14, 21, 28, 35 and 42, from batches suitably infected with D. caninum metacestodes. Prior to each post-treatment infestation the D. caninum infection rate for the fleas was determined by microscopically examining 100 fleas for D. caninum metacestodes. The D. caninum prevalence in the fleas used for infestations ranged from 23 % to 52 %. Medicated collars were fitted to 8 of the dogs on study day 0. The weight of the IVP collars varied between 35.48 g and 38.48 g (average 37.16 g), whilst animal weight varied between 12.20 kg and 17.98 kg (treated group, n = 8, average 14.79 kg). Seven days later infestation of each of the 16 dogs with 250 fleas commenced. Infestations continued at weekly intervals until Day 42 with efficacy against fleas evaluated 24 hours after each infestation. From Days 21 to 74, infection of the dogs with D. caninum was verified (daily examination of faeces and cages for the presence of expelled proglottids). Calculation of prophylactic effectiveness of the collars in preventing infection with D. caninum was based on the difference in geometric mean numbers of scoleces between groups at necropsy on Day 75. Effective prevention of infection with D. caninum was found to be 96.6 %. Efficacy of the collars against fleas was ≥ 99.9 % for the duration of the assessment period. Newly acquired infestations of fleas are rapidly eliminated by the insecticidal components of the medicated collars over a period of several months. In the event of fleas being infected with metacestodes, with D. caninum can be prevented in collared dogs, concurrently reducing the likelihood of transmission to humans.

Juliana Milani Araujo - One of the best experts on this subject based on the ideXlab platform.

  • activity of the nematophagous fungi pochonia chlamydosporia duddingtonia flagrans and monacrosporium thaumasium on egg capsules of Dipylidium caninum
    Veterinary Parasitology, 2009
    Co-Authors: Juliana Milani Araujo, Fabio Ribeiro Braga, Jackson Victor De Araujo, Rogerio Oliva Carvalho, Sebastiao Rodrigo Ferreira
    Abstract:

    Nematophagous fungi are potential biological control agents of helminths. The in vitro ovicidal effect of four isolates of the nematophagous fungi Pochonia chlamydosporia (VC1 and VC4), Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) was evaluated on egg capsules of Dipylidium caninum, a cestode parasite of dogs, cats and humans. One thousand egg capsules of D. caninum were plated on 2% water-agar with the grown isolates and control without fungus. The ovicidal activity of these fungi was evaluated 5, 10 and 15 days after incubation. Only P. chlamydosporia showed ovicidal activity (p<0.05) on D. caninum egg capsules, of 19.6% (VC1) and 20% (VC4) on the 5th day; 44.2% (VC1) and 31.5% (VC4) on the 10th day; and 49.2% (VC1) and 41.9% (VC4) on the 15th day. D. flagrans and M. thaumasium caused no morphological damage to egg capsules. The results demonstrated that P. chlamydosporia was in vitro effective against capsules and eggs of D. caninum, and can be considered as a potential biological control agent for this helminth.

  • atividade dos fungos nematofagos pochonia chlamydosporia e paecilomyces lilacinus sobre capsulas de ovos de Dipylidium caninum
    Revista do Instituto Adolfo Lutz (Impresso), 2009
    Co-Authors: Juliana Milani Araujo, Fabio Ribeiro Braga, Jackson Victor De Araujo, Rogerio Oliva Carvalho
    Abstract:

    Nematophagous fungi are potential agents to be employed for biological control of helminthes. The ovicidal activity of the nematophagous fungi Pochonia chlamydosporia (isolates VC1 and VC4) and Paecilomyces lilacinus on egg capsules of Dipylidium caninum, a cestoda parasite of dogs, cats and men, was evaluated on Petri dishes cultures. One thousand of D. caninum egg capsules were placed onto Petri dishes containing 2% water-agar medium and grown fungal isolates, and also onto dishes without fungi, as control. The ovicidal activity of these fungi was evaluated after 5, 10 and 15 days. After the beginning of the interaction and at the end of the experiment, fungi P. chlamydosporia and Paecilomyces lilacinus demonstrated ovicidal activity (p<0.05) when compared to the control. Pochonia. chlamydosporia showed ovicidal activity of 49.0% (isolate VC1) and 41.9% (isolate VC4), while ovicidal activity of Paecilomyces lilacinus was 42.7% after fifteen days of interaction. The fungi Pochonia chlamydosporia and Paecilomyces lilacinus showed ovicidal activity on Dipylidium caninum egg capsules, thus it could be used as potential biological controllers of this cestoda.

  • activity of nematophagous fungi pochonia chlamydosporia and paecilomyces lilacinus on Dipylidium caninum egg capsules atividade dos fungos nematofagos pochonia chlamydosporia e paecilomyces lilacinus sobre capsulas de ovos de Dipylidium caninum
    2009
    Co-Authors: Juliana Milani Araujo, Fabio Ribeiro Braga, Jackson Victor De Araujo, Rogerio Oliva
    Abstract:

    Nematophagous fungi are potential agents to be employed for biological control of helminthes. The ovicidal activity of the nematophagous fungi Pochonia chlamydosporia (isolates VC1 and VC4) and Paecilomyces lilacinus on egg capsules of Dipylidium caninum, a cestoda parasite of dogs, cats and men, was evaluated on Petri dishes cultures. One thousand of D. caninum egg capsules were placed onto Petri dishes containing 2% water-agar medium and grown fungal isolates, and also onto dishes without fungi, as control. The ovicidal activity of these fungi was evaluated after 5, 10 and 15 days. After the beginning of the interaction and at the end of the experiment, fungi P. chlamydosporia and Paecilomyces lilacinus demonstrated ovicidal activity (p<0.05) when compared to the control. Pochonia. chlamydosporia showed ovicidal activity of 49.0% (isolate VC1) and 41.9% (isolate VC4), while ovicidal activity of Paecilomyces lilacinus was 42.7% after fi fteen days of interaction. The fungi Pochonia chlamydosporia and Paecilomyces lilacinus showed ovicidal activity on Dipylidium caninum egg capsules, thus it could be used as potential biological controllers of this cestoda.

Rogerio Oliva Carvalho - One of the best experts on this subject based on the ideXlab platform.

  • activity of the nematophagous fungi pochonia chlamydosporia duddingtonia flagrans and monacrosporium thaumasium on egg capsules of Dipylidium caninum
    Veterinary Parasitology, 2009
    Co-Authors: Juliana Milani Araujo, Fabio Ribeiro Braga, Jackson Victor De Araujo, Rogerio Oliva Carvalho, Sebastiao Rodrigo Ferreira
    Abstract:

    Nematophagous fungi are potential biological control agents of helminths. The in vitro ovicidal effect of four isolates of the nematophagous fungi Pochonia chlamydosporia (VC1 and VC4), Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) was evaluated on egg capsules of Dipylidium caninum, a cestode parasite of dogs, cats and humans. One thousand egg capsules of D. caninum were plated on 2% water-agar with the grown isolates and control without fungus. The ovicidal activity of these fungi was evaluated 5, 10 and 15 days after incubation. Only P. chlamydosporia showed ovicidal activity (p<0.05) on D. caninum egg capsules, of 19.6% (VC1) and 20% (VC4) on the 5th day; 44.2% (VC1) and 31.5% (VC4) on the 10th day; and 49.2% (VC1) and 41.9% (VC4) on the 15th day. D. flagrans and M. thaumasium caused no morphological damage to egg capsules. The results demonstrated that P. chlamydosporia was in vitro effective against capsules and eggs of D. caninum, and can be considered as a potential biological control agent for this helminth.

  • atividade dos fungos nematofagos pochonia chlamydosporia e paecilomyces lilacinus sobre capsulas de ovos de Dipylidium caninum
    Revista do Instituto Adolfo Lutz (Impresso), 2009
    Co-Authors: Juliana Milani Araujo, Fabio Ribeiro Braga, Jackson Victor De Araujo, Rogerio Oliva Carvalho
    Abstract:

    Nematophagous fungi are potential agents to be employed for biological control of helminthes. The ovicidal activity of the nematophagous fungi Pochonia chlamydosporia (isolates VC1 and VC4) and Paecilomyces lilacinus on egg capsules of Dipylidium caninum, a cestoda parasite of dogs, cats and men, was evaluated on Petri dishes cultures. One thousand of D. caninum egg capsules were placed onto Petri dishes containing 2% water-agar medium and grown fungal isolates, and also onto dishes without fungi, as control. The ovicidal activity of these fungi was evaluated after 5, 10 and 15 days. After the beginning of the interaction and at the end of the experiment, fungi P. chlamydosporia and Paecilomyces lilacinus demonstrated ovicidal activity (p<0.05) when compared to the control. Pochonia. chlamydosporia showed ovicidal activity of 49.0% (isolate VC1) and 41.9% (isolate VC4), while ovicidal activity of Paecilomyces lilacinus was 42.7% after fifteen days of interaction. The fungi Pochonia chlamydosporia and Paecilomyces lilacinus showed ovicidal activity on Dipylidium caninum egg capsules, thus it could be used as potential biological controllers of this cestoda.

Fabio Ribeiro Braga - One of the best experts on this subject based on the ideXlab platform.

  • activity of the nematophagous fungi pochonia chlamydosporia duddingtonia flagrans and monacrosporium thaumasium on egg capsules of Dipylidium caninum
    Veterinary Parasitology, 2009
    Co-Authors: Juliana Milani Araujo, Fabio Ribeiro Braga, Jackson Victor De Araujo, Rogerio Oliva Carvalho, Sebastiao Rodrigo Ferreira
    Abstract:

    Nematophagous fungi are potential biological control agents of helminths. The in vitro ovicidal effect of four isolates of the nematophagous fungi Pochonia chlamydosporia (VC1 and VC4), Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) was evaluated on egg capsules of Dipylidium caninum, a cestode parasite of dogs, cats and humans. One thousand egg capsules of D. caninum were plated on 2% water-agar with the grown isolates and control without fungus. The ovicidal activity of these fungi was evaluated 5, 10 and 15 days after incubation. Only P. chlamydosporia showed ovicidal activity (p<0.05) on D. caninum egg capsules, of 19.6% (VC1) and 20% (VC4) on the 5th day; 44.2% (VC1) and 31.5% (VC4) on the 10th day; and 49.2% (VC1) and 41.9% (VC4) on the 15th day. D. flagrans and M. thaumasium caused no morphological damage to egg capsules. The results demonstrated that P. chlamydosporia was in vitro effective against capsules and eggs of D. caninum, and can be considered as a potential biological control agent for this helminth.

  • atividade dos fungos nematofagos pochonia chlamydosporia e paecilomyces lilacinus sobre capsulas de ovos de Dipylidium caninum
    Revista do Instituto Adolfo Lutz (Impresso), 2009
    Co-Authors: Juliana Milani Araujo, Fabio Ribeiro Braga, Jackson Victor De Araujo, Rogerio Oliva Carvalho
    Abstract:

    Nematophagous fungi are potential agents to be employed for biological control of helminthes. The ovicidal activity of the nematophagous fungi Pochonia chlamydosporia (isolates VC1 and VC4) and Paecilomyces lilacinus on egg capsules of Dipylidium caninum, a cestoda parasite of dogs, cats and men, was evaluated on Petri dishes cultures. One thousand of D. caninum egg capsules were placed onto Petri dishes containing 2% water-agar medium and grown fungal isolates, and also onto dishes without fungi, as control. The ovicidal activity of these fungi was evaluated after 5, 10 and 15 days. After the beginning of the interaction and at the end of the experiment, fungi P. chlamydosporia and Paecilomyces lilacinus demonstrated ovicidal activity (p<0.05) when compared to the control. Pochonia. chlamydosporia showed ovicidal activity of 49.0% (isolate VC1) and 41.9% (isolate VC4), while ovicidal activity of Paecilomyces lilacinus was 42.7% after fifteen days of interaction. The fungi Pochonia chlamydosporia and Paecilomyces lilacinus showed ovicidal activity on Dipylidium caninum egg capsules, thus it could be used as potential biological controllers of this cestoda.

  • activity of nematophagous fungi pochonia chlamydosporia and paecilomyces lilacinus on Dipylidium caninum egg capsules atividade dos fungos nematofagos pochonia chlamydosporia e paecilomyces lilacinus sobre capsulas de ovos de Dipylidium caninum
    2009
    Co-Authors: Juliana Milani Araujo, Fabio Ribeiro Braga, Jackson Victor De Araujo, Rogerio Oliva
    Abstract:

    Nematophagous fungi are potential agents to be employed for biological control of helminthes. The ovicidal activity of the nematophagous fungi Pochonia chlamydosporia (isolates VC1 and VC4) and Paecilomyces lilacinus on egg capsules of Dipylidium caninum, a cestoda parasite of dogs, cats and men, was evaluated on Petri dishes cultures. One thousand of D. caninum egg capsules were placed onto Petri dishes containing 2% water-agar medium and grown fungal isolates, and also onto dishes without fungi, as control. The ovicidal activity of these fungi was evaluated after 5, 10 and 15 days. After the beginning of the interaction and at the end of the experiment, fungi P. chlamydosporia and Paecilomyces lilacinus demonstrated ovicidal activity (p<0.05) when compared to the control. Pochonia. chlamydosporia showed ovicidal activity of 49.0% (isolate VC1) and 41.9% (isolate VC4), while ovicidal activity of Paecilomyces lilacinus was 42.7% after fi fteen days of interaction. The fungi Pochonia chlamydosporia and Paecilomyces lilacinus showed ovicidal activity on Dipylidium caninum egg capsules, thus it could be used as potential biological controllers of this cestoda.