The Experts below are selected from a list of 1353 Experts worldwide ranked by ideXlab platform
Teruhiko Nitoda - One of the best experts on this subject based on the ideXlab platform.
-
A new asteltoxin analog with insecticidal activity from Pochonia suchlasporia TAMA 87.
Journal of Pesticide Science, 2020Co-Authors: Syaefudin Suminto, Eri Takatsuji, Ayako Iguchi, Hiroshi Kanzaki, Toru Okuda, Teruhiko NitodaAbstract:A new asteltoxin analog, named asteltoxin H (1), was isolated by the solid-state fermentation of the fungus Pochonia suchlasporia var. suchlasporia TAMA 87. The chemical structure of 1 was deduced by spectroscopic methods, including 1D and 2D NMR, HRESIMS, and UV-Vis analyses. Compound 1 showed insecticidal activity against prepupae of the blowfly, Lucilia sericata, with an LD50 value of 0.94 µg/mg prepupal body weight.
-
pochonicine a polyhydroxylated pyrrolizidine alkaloid from fungus Pochonia suchlasporia var suchlasporia tama 87 as a potent β n acetylglucosaminidase inhibitor
Bioorganic & Medicinal Chemistry, 2009Co-Authors: Hirokazu Usuki, Hiroshi Kanzaki, Toru Okuda, Miho Toyooka, Teruhiko NitodaAbstract:A new polyhydroxylated pyrrolizidine alkaloid designated as pochonicine (1) was isolated from a solid fermentation culture of the fungal strain Pochonia suchlasporia var. suchlasporia TAMA 87. The structure of 1 was determined using NMR and MS techniques as (1R*, 3S*, 5S*, 6S*, 7R*, 7a S*)-5-acetamidomethyl-3-hydroxymethyl-1,6,7-trihydroxypyrrolizidine. Pochonicine (1) showed potent inhibition against beta-N-acetylglucosaminidases (GlcNAcases) of various organisms including insects, fungi, mammals, and a plant but no inhibition against beta-glucosidase of almond, alpha-glucosidase of yeast, or chitinase of Bacillus sp. The GlcNAcase inhibitory activity of pochonicine (1) was comparable to nagstatin, a potent GlcNAcase inhibitor of natural origin.
Jackson Victor De Araújo - One of the best experts on this subject based on the ideXlab platform.
-
Arthrobotrys cladodes and Pochonia chlamydosporia: Nematicidal effects of single and combined use after passage through cattle gastrointestinal tract.
Experimental Parasitology, 2020Co-Authors: Ítalo Stoupa Vieira, Isabela De Castro Oliveira, Artur Kanadani Campos, Jackson Victor De AraújoAbstract:Abstract Nematodiosis are responsible for financial losses in cattle production systems due to treatment costs, falling production rates and animal deaths. The incorporation of sodium alginate pellets containing nematophagous fungi in the bovine diet is a method for the control of nematodiosis. The aims of this study were to evaluate the viability of Arthrobotrys cladodes and Pochonia chlamydosporia contained in sodium alginate pellets after passage through the bovine gastrointestinal tract, as well as to evaluate the effects of single and combined use of these fungi against bovine parasitic nematodes. The reduction in parasitic nematode infective larvae of bovines by the combined use of Arthrobotrys cladodes and Pochonia chlamydosporia was more than the reduction in infective larvae by Arthrobotrys cladodes or Pochonia chlamydosporia alone in the collections 12, 24 and 36 h after giving the pellets to the animals. Arthrobotrys cladodes and Pochonia chlamydosporia combined promoted maximum reduction of infective larvae of 86.3%. Therefore, the combination of Arthrobotrys cladodes and Pochonia chlamydosporia may be an effective method to control gastrointestinal nematodiosis affecting grazing cattle.
-
Fungi predatory activity on embryonated Toxocara canis eggs inoculated in domestic chickens (Gallus gallus domesticus) and destruction of second stage larvae
Parasitology Research, 2015Co-Authors: Emy Hiura, Filippe Elias De Freitas Soares, Leandro Abreu Da Fonseca, Aline Carmen Garcia Lopes, Jeanne Saraiva Paz, Maylla Garschagen Gava, Mayra Cunha Flecher, Manuela Colares, Tracy Lacerda, Jackson Victor De AraújoAbstract:The objective of this study was to evaluate the infectivity of Toxocara canis eggs after interacting with isolated nematophagous fungi of the species Duddingtonia flagrans (AC001) and Pochonia chlamydosporia (VC4), and test the predatory activity of the isolated AC001 on T. canis second stage larvae after 7 days of interaction. In assay A, 5000 embryonated T. canis eggs previously in contact with the AC001 and VC4 isolated for 10 days were inoculated into domestic chickens ( Gallus gallus domesticus ), and then these animals were necropsied to collect material (digested liver, intestine, muscles and lungs) at 3-, 7-, 14-, and 21-day intervals after inoculation. In assay A, the results demonstrated that the prior interaction of the eggs with isolated AC001 and VC4 decreases the amount of larvae found in the collected organs. Difference ( p
-
Action of the nematophagous fungus Pochonia chlamydosporia on Dioctophyma renale eggs
Biocontrol Science and Technology, 2014Co-Authors: Filippe Elias De Freitas Soares, Fabio Ribeiro Braga, Juliana Milani Araujo, José Humberto De Queiroz, Alexandre De Oliveira Tavela, Angélica De Souza Gouveia, Leandro Abreu Da Fonseca, Jackson Victor De AraújoAbstract:The ovicidal action of the nematophagous fungus Pochonia chlamydosporia (VC4) was evaluated on Dioctophyma renale eggs under laboratory conditions (Assay A). Next, the enzymatic action of proteases and chitinases produced by P. chlamydosporia (VC4) was evaluated on D. renale eggs, under laboratory conditions (Assay B). At the end of the experiment, there was difference (p 0.01) among the concentrations in the destruction of eggs. However, there was a trend of increasing mortality with increased concentration. Then (Assay B), it was observed that in the 24-hour interval, the proteases and chitinases of P. chlamydosporia (VC4), either individually or together, caused a significant percentage reduction (p < 0.01) on the number of viable eggs of D. renale, compared to control, with the following reduction values: 27.8% (proteases), 29.4%...
-
enzymatic analysis and in vitro ovicidal effect of Pochonia chlamydosporia and paecilomyces lilacinus on oxyuris equi eggs of horses
Biocontrol Science and Technology, 2012Co-Authors: Fabio Ribeiro Braga, Jackson Victor De Araújo, Juliana Milani Araujo, Rogério Oliva Carvalho, Filippe Elias De Freitas Soares, Alexandre De Oliveira Tavela, Fernanda Mara Fernandes, José Humberto De QueirozAbstract:Abstract The aim of this work was to analyse the enzymatic activity and in vitro ovicidal effect of Pochonia chlamydosporia (VC1 and VC4) and Paecilomyces lilacinus (PL1) on Oxyuris equi eggs of horses. The growth of isolates and their enzymatic production were evaluated on agar media supplemented with gelatin (GA), casein (CA), olive oil (OOA) or starch (SSA). The ovicidal effect was evaluated according to morphological alterations. Following, the P. chlamydosporia crude extract production and proteolytic content was evaluated (VC1 and VC4) in liquid medium at the interval of 15, 30, 45 and 60 min during incubation of P. chlamydosporia and P. lilacinus which grew and showed enzymatic activity on agar media (GA, CA, OOA and SSA). At the 15th day, VC1, VC4 and PL1 showed results on GA, CA, OOA and SSA media, for Type 3 effect of (78, 54, 52 and 68%), (72, 50, 58 and 78%) and (62, 57, 65 and 63%). Pochonia chlamydosporia was able to grow in liquid medium (gelatin) and at Day 5 showed proteolytic activity. T...
-
In vitro association of nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Pochonia chlamydosporia (VC1) to control horse cyathostomin (Nematoda: Strongylidae)
Biocontrol Science and Technology, 2012Co-Authors: Alexandre De Oliveira Tavela, Jackson Victor De Araújo, Fabio Ribeiro Braga, Juliana Milani Araujo, L.q. Magalhães, Wendeo Ferreira Da Silveira, Luana Alcântara BorgesAbstract:Abstract In vitro effects of nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Pochonia chlamydosporia (VC1) were evaluated against eggs and third-stage infective larvae (L3) of horse cyathostomin (Nematoda: Strongylidae). The following percentage reductions compared with the control group were observed after a 20-day exposure period: AC001, 61.6%; NF34, 66.1%; VC1, 73.2%; group AC001 + VC1, 86.8%; NF34 + VC1, 77.3%; AC001 + NF34, 92.4%. The results showed that the fungal isolates (VC1, AC001 and NF34), acting alone or in conjunction, were efficient in controlling horse cyathostomin under in vitro conditions.
Akira Kawashima - One of the best experts on this subject based on the ideXlab platform.
-
13 bromomonocillin i a new wnt 5a expression inhibitor produced by Pochonia chlamydosporia var chlamydosporia
Heterocycles, 2009Co-Authors: Hideki Shinonaga, Akiko Ikeda, Mari Aoki, Noriyoshi Sakai, Yuriko Nozawa, Akira KawashimaAbstract:WNT-5A is a secretory glycoprotein related to the proliferation of dermal papilla cells. While searching for an inhibitor of WNT-5A expression, a new compound, 13-bromomonocillin I (1), was isolated from the fermentation broth of the fungus Pochonia chlamydosporia, cultured in NaBr-supplemented medium. 13-Bromomonocillin I (1) exhibited WNT-5A expression inhibitory activity with an IC 50 value of 0.25 μM.
-
pochonins k p new radicicol analogues from Pochonia chlamydosporia var chlamydosporia and their wnt 5a expression inhibitory activities
Tetrahedron, 2009Co-Authors: Hideki Shinonaga, Yoji Kawamura, Akiko Ikeda, Mari Aoki, Noriyoshi Sakai, Natsuko Fujimoto, Akira KawashimaAbstract:Abstract WNT-5A, a secretory glycoprotein, is related to the proliferation of dermal papilla cells. To develop a hair-growth stimulant, we have been searching for an inhibitor of WNT-5A expression, and have identified an active compound, radicicol ( 1 ), and isolated six new radicicol analogues, pochonins K–P ( 2 – 7 ), together with ten known radicicol analogues, including monorden analogue-1 ( 8 ), pochonin E ( 9 ), and pochonin F ( 10 ), from a culture broth of the fungus Pochonia chlamydosporia TF-0480. This report describes the structural elucidation of 2 – 7 , determination of the stereochemistries of 8 – 10 , and their biological activities against WNT-5A.
-
Pochonins K–P: new radicicol analogues from Pochonia chlamydosporia var. chlamydosporia and their WNT-5A expression inhibitory activities
Tetrahedron, 2009Co-Authors: Hideki Shinonaga, Yoji Kawamura, Akiko Ikeda, Mari Aoki, Noriyoshi Sakai, Natsuko Fujimoto, Akira KawashimaAbstract:Abstract WNT-5A, a secretory glycoprotein, is related to the proliferation of dermal papilla cells. To develop a hair-growth stimulant, we have been searching for an inhibitor of WNT-5A expression, and have identified an active compound, radicicol ( 1 ), and isolated six new radicicol analogues, pochonins K–P ( 2 – 7 ), together with ten known radicicol analogues, including monorden analogue-1 ( 8 ), pochonin E ( 9 ), and pochonin F ( 10 ), from a culture broth of the fungus Pochonia chlamydosporia TF-0480. This report describes the structural elucidation of 2 – 7 , determination of the stereochemistries of 8 – 10 , and their biological activities against WNT-5A.
-
The search for a hair-growth stimulant: new radicicol analogues as WNT-5A expression inhibitors from Pochonia chlamydosporia var. chlamydosporia
Tetrahedron Letters, 2009Co-Authors: Hideki Shinonaga, Yoji Kawamura, Akiko Ikeda, Mari Aoki, Noriyoshi Sakai, Natsuko Fujimoto, Akira KawashimaAbstract:Abstract WNT-5A, a secretory glycoprotein, is related to the proliferation of dermal papilla cells. While searching for an inhibitor of WNT-5A expression, we identified an active compound, radicicol ( 1 ), and isolated four unique analogues, pochonins G–J ( 2 – 5 ), from a culture broth of the fungus Pochonia chlamydosporia TF-0480. Structural elucidation of 2 – 5 and their biological activities against WNT-5A are described.
Hideki Shinonaga - One of the best experts on this subject based on the ideXlab platform.
-
13 bromomonocillin i a new wnt 5a expression inhibitor produced by Pochonia chlamydosporia var chlamydosporia
Heterocycles, 2009Co-Authors: Hideki Shinonaga, Akiko Ikeda, Mari Aoki, Noriyoshi Sakai, Yuriko Nozawa, Akira KawashimaAbstract:WNT-5A is a secretory glycoprotein related to the proliferation of dermal papilla cells. While searching for an inhibitor of WNT-5A expression, a new compound, 13-bromomonocillin I (1), was isolated from the fermentation broth of the fungus Pochonia chlamydosporia, cultured in NaBr-supplemented medium. 13-Bromomonocillin I (1) exhibited WNT-5A expression inhibitory activity with an IC 50 value of 0.25 μM.
-
pochonins k p new radicicol analogues from Pochonia chlamydosporia var chlamydosporia and their wnt 5a expression inhibitory activities
Tetrahedron, 2009Co-Authors: Hideki Shinonaga, Yoji Kawamura, Akiko Ikeda, Mari Aoki, Noriyoshi Sakai, Natsuko Fujimoto, Akira KawashimaAbstract:Abstract WNT-5A, a secretory glycoprotein, is related to the proliferation of dermal papilla cells. To develop a hair-growth stimulant, we have been searching for an inhibitor of WNT-5A expression, and have identified an active compound, radicicol ( 1 ), and isolated six new radicicol analogues, pochonins K–P ( 2 – 7 ), together with ten known radicicol analogues, including monorden analogue-1 ( 8 ), pochonin E ( 9 ), and pochonin F ( 10 ), from a culture broth of the fungus Pochonia chlamydosporia TF-0480. This report describes the structural elucidation of 2 – 7 , determination of the stereochemistries of 8 – 10 , and their biological activities against WNT-5A.
-
Pochonins K–P: new radicicol analogues from Pochonia chlamydosporia var. chlamydosporia and their WNT-5A expression inhibitory activities
Tetrahedron, 2009Co-Authors: Hideki Shinonaga, Yoji Kawamura, Akiko Ikeda, Mari Aoki, Noriyoshi Sakai, Natsuko Fujimoto, Akira KawashimaAbstract:Abstract WNT-5A, a secretory glycoprotein, is related to the proliferation of dermal papilla cells. To develop a hair-growth stimulant, we have been searching for an inhibitor of WNT-5A expression, and have identified an active compound, radicicol ( 1 ), and isolated six new radicicol analogues, pochonins K–P ( 2 – 7 ), together with ten known radicicol analogues, including monorden analogue-1 ( 8 ), pochonin E ( 9 ), and pochonin F ( 10 ), from a culture broth of the fungus Pochonia chlamydosporia TF-0480. This report describes the structural elucidation of 2 – 7 , determination of the stereochemistries of 8 – 10 , and their biological activities against WNT-5A.
-
The search for a hair-growth stimulant: new radicicol analogues as WNT-5A expression inhibitors from Pochonia chlamydosporia var. chlamydosporia
Tetrahedron Letters, 2009Co-Authors: Hideki Shinonaga, Yoji Kawamura, Akiko Ikeda, Mari Aoki, Noriyoshi Sakai, Natsuko Fujimoto, Akira KawashimaAbstract:Abstract WNT-5A, a secretory glycoprotein, is related to the proliferation of dermal papilla cells. While searching for an inhibitor of WNT-5A expression, we identified an active compound, radicicol ( 1 ), and isolated four unique analogues, pochonins G–J ( 2 – 5 ), from a culture broth of the fungus Pochonia chlamydosporia TF-0480. Structural elucidation of 2 – 5 and their biological activities against WNT-5A are described.
Fabio Ribeiro Braga - One of the best experts on this subject based on the ideXlab platform.
-
Action of the nematophagous fungus Pochonia chlamydosporia on Dioctophyma renale eggs
Biocontrol Science and Technology, 2014Co-Authors: Filippe Elias De Freitas Soares, Fabio Ribeiro Braga, Juliana Milani Araujo, José Humberto De Queiroz, Alexandre De Oliveira Tavela, Angélica De Souza Gouveia, Leandro Abreu Da Fonseca, Jackson Victor De AraújoAbstract:The ovicidal action of the nematophagous fungus Pochonia chlamydosporia (VC4) was evaluated on Dioctophyma renale eggs under laboratory conditions (Assay A). Next, the enzymatic action of proteases and chitinases produced by P. chlamydosporia (VC4) was evaluated on D. renale eggs, under laboratory conditions (Assay B). At the end of the experiment, there was difference (p 0.01) among the concentrations in the destruction of eggs. However, there was a trend of increasing mortality with increased concentration. Then (Assay B), it was observed that in the 24-hour interval, the proteases and chitinases of P. chlamydosporia (VC4), either individually or together, caused a significant percentage reduction (p < 0.01) on the number of viable eggs of D. renale, compared to control, with the following reduction values: 27.8% (proteases), 29.4%...
-
enzymatic analysis and in vitro ovicidal effect of Pochonia chlamydosporia and paecilomyces lilacinus on oxyuris equi eggs of horses
Biocontrol Science and Technology, 2012Co-Authors: Fabio Ribeiro Braga, Jackson Victor De Araújo, Juliana Milani Araujo, Rogério Oliva Carvalho, Filippe Elias De Freitas Soares, Alexandre De Oliveira Tavela, Fernanda Mara Fernandes, José Humberto De QueirozAbstract:Abstract The aim of this work was to analyse the enzymatic activity and in vitro ovicidal effect of Pochonia chlamydosporia (VC1 and VC4) and Paecilomyces lilacinus (PL1) on Oxyuris equi eggs of horses. The growth of isolates and their enzymatic production were evaluated on agar media supplemented with gelatin (GA), casein (CA), olive oil (OOA) or starch (SSA). The ovicidal effect was evaluated according to morphological alterations. Following, the P. chlamydosporia crude extract production and proteolytic content was evaluated (VC1 and VC4) in liquid medium at the interval of 15, 30, 45 and 60 min during incubation of P. chlamydosporia and P. lilacinus which grew and showed enzymatic activity on agar media (GA, CA, OOA and SSA). At the 15th day, VC1, VC4 and PL1 showed results on GA, CA, OOA and SSA media, for Type 3 effect of (78, 54, 52 and 68%), (72, 50, 58 and 78%) and (62, 57, 65 and 63%). Pochonia chlamydosporia was able to grow in liquid medium (gelatin) and at Day 5 showed proteolytic activity. T...
-
In vitro association of nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Pochonia chlamydosporia (VC1) to control horse cyathostomin (Nematoda: Strongylidae)
Biocontrol Science and Technology, 2012Co-Authors: Alexandre De Oliveira Tavela, Jackson Victor De Araújo, Fabio Ribeiro Braga, Juliana Milani Araujo, L.q. Magalhães, Wendeo Ferreira Da Silveira, Luana Alcântara BorgesAbstract:Abstract In vitro effects of nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Pochonia chlamydosporia (VC1) were evaluated against eggs and third-stage infective larvae (L3) of horse cyathostomin (Nematoda: Strongylidae). The following percentage reductions compared with the control group were observed after a 20-day exposure period: AC001, 61.6%; NF34, 66.1%; VC1, 73.2%; group AC001 + VC1, 86.8%; NF34 + VC1, 77.3%; AC001 + NF34, 92.4%. The results showed that the fungal isolates (VC1, AC001 and NF34), acting alone or in conjunction, were efficient in controlling horse cyathostomin under in vitro conditions.
-
Biological control of Ascaris suum eggs by Pochonia chlamydosporia fungus
Veterinary Research Communications, 2011Co-Authors: Sebastião Rodrigo Ferreira, Jackson Victor De Araújo, Fabio Ribeiro Braga, Juliana Milani Araujo, Luiza Neme Frassy, Aloízio Soares FerreiraAbstract:Ascaris suum is a gastrointestinal nematode parasite of swines. The aim of this study was to observe Pochonia chlamydosporia fungus on biological control of A. suum eggs after fungus passage through swines gastrointestinal tract. Eighteen pigs, previously dewormed, were randomly divided into three groups: group 1, treated with the fungus isolate VC4; group 2, treated with the fungus isolate VC1 and group 3 did not receive fungus (control). In the treated groups, each animal received a 9 g single dose of mycelium mass containing P. chlamydosporia (VC1 or VC4). Thereafter, animal fecal samples were collected at the following intervals: 8, 12, 24, 36, 48, 72 and 96 h after treatment beginning and these were poured in Petri dishes containing 2% water-agar culture medium. Then, 1,000 A. suum eggs were poured into each dish and kept in an incubator at 26°C and in the dark for 30 days. After this period, approximately 100 eggs were removed from each Petri dish and morphologically analyzed under light microscopy following the ovicidal activity parameters. The higher percentage observed for isolated VC4 eggs destruction was 57.5% (36 h) after fungus administration and for isolate VC1 this percentage was 45.8% (24 h and 72 h) (p > 0.01). P. chlamydosporia remained viable after passing through the gastrointestinal tract of swines, maintaining its ability of destroying A. suum eggs.
-
Ovicidal action of a crude enzymatic extract of fungus Pochonia chlamydosporia against Ancylostoma sp eggs
Revista da Sociedade Brasileira de Medicina Tropical, 2011Co-Authors: Fabio Ribeiro Braga, Jackson Victor De Araújo, Juliana Milani Araujo, André R. Silva, Rogério Oliva Carvalho, Filippe Elias De Freitas Soares, José Humberto De Queiroz, Hugo Leonardo André GenierAbstract:INTRODUCTION: Ancylostoma sp is a potentially zoonotic geohelminth. METHODS: This study aimed to evaluate in vitro the action of crude enzyme extract of Pochonia chlamydosporia (VC4) on eggs of Ancylostoma sp in 2% water-agar and in fecal cultures. RESULTS: The percentage reduction in Ancylostoma sp egg eclosion was 76.8% in Petri dishes of the treated group compared to the control group. CONCLUSIONS: The crude enzyme extract of Pochonia chlamydosporia was effective at reducing Ancylostoma sp egg eclosion and can be used as biological control of this nematode.