The Experts below are selected from a list of 498816 Experts worldwide ranked by ideXlab platform
Dietmar W. Hutmacher - One of the best experts on this subject based on the ideXlab platform.
-
In vitro Disease Models 4.0 via automation and high-throughput processing.
Biofabrication, 2019Co-Authors: Sebastian Eggert, Dietmar W. HutmacherAbstract:While much progress has been accomplished in the development of physiologically relevant in vitro Disease Models, current manufacturing and characterisation workflows still rely on manual, time-consuming, and low-throughput processes, which are not efficient and prone to human errors. For these reasons adoption and, more importantly, reproducibility and validation of 3D in vitro Disease Models is rather low for fundamental and applied research concepts. This article argues in form of a perspective view that automation and high-throughput methodologies will play a vital role to act as a catalyst to accelerate the development and characterisation process for generations to come. Innovative engineering concepts are required to overcome current limitations of in vitro Disease Models and to foster the scientific rigour as well as the applied research potential.
Luke A Macqueen - One of the best experts on this subject based on the ideXlab platform.
-
engineered in vitro Disease Models
Annual Review of Pathology-mechanisms of Disease, 2015Co-Authors: Kambez H Benam, Stephanie Dauth, Bryan Hassell, Anna Herland, Abhishek Jain, Kyungjin Jang, Katia Karalis, Hyun Jung Kim, Luke A MacqueenAbstract:The ultimate goal of most biomedical research is to gain greater insight into mechanisms of human Disease or to develop new and improved therapies or diagnostics. Although great advances have been made in terms of developing Disease Models in animals, such as transgenic mice, many of these Models fail to faithfully recapitulate the human condition. In addition, it is difficult to identify critical cellular and molecular contributors to Disease or to vary them independently in whole-animal Models. This challenge has attracted the interest of engineers, who have begun to collaborate with biologists to leverage recent advances in tissue engineering and microfabrication to develop novel in vitro Models of Disease. As these Models are synthetic systems, specific molecular factors and individual cell types, including parenchymal cells, vascular cells, and immune cells, can be varied independently while simultaneously measuring system-level responses in real time. In this article, we provide some examples of these efforts, including engineered Models of Diseases of the heart, lung, intestine, liver, kidney, cartilage, skin and vascular, endocrine, musculoskeletal, and nervous systems, as well as Models of infectious Diseases and cancer. We also describe how engineered in vitro Models can be combined with human inducible pluripotent stem cells to enable new insights into a broad variety of Disease mechanisms, as well as provide a test bed for screening new therapies.
Katia Karalis - One of the best experts on this subject based on the ideXlab platform.
-
engineered in vitro Disease Models
Annual Review of Pathology-mechanisms of Disease, 2015Co-Authors: Kambez H Benam, Stephanie Dauth, Bryan Hassell, Anna Herland, Abhishek Jain, Kyungjin Jang, Katia Karalis, Hyun Jung Kim, Luke A MacqueenAbstract:The ultimate goal of most biomedical research is to gain greater insight into mechanisms of human Disease or to develop new and improved therapies or diagnostics. Although great advances have been made in terms of developing Disease Models in animals, such as transgenic mice, many of these Models fail to faithfully recapitulate the human condition. In addition, it is difficult to identify critical cellular and molecular contributors to Disease or to vary them independently in whole-animal Models. This challenge has attracted the interest of engineers, who have begun to collaborate with biologists to leverage recent advances in tissue engineering and microfabrication to develop novel in vitro Models of Disease. As these Models are synthetic systems, specific molecular factors and individual cell types, including parenchymal cells, vascular cells, and immune cells, can be varied independently while simultaneously measuring system-level responses in real time. In this article, we provide some examples of these efforts, including engineered Models of Diseases of the heart, lung, intestine, liver, kidney, cartilage, skin and vascular, endocrine, musculoskeletal, and nervous systems, as well as Models of infectious Diseases and cancer. We also describe how engineered in vitro Models can be combined with human inducible pluripotent stem cells to enable new insights into a broad variety of Disease mechanisms, as well as provide a test bed for screening new therapies.
Sebastian Eggert - One of the best experts on this subject based on the ideXlab platform.
-
In vitro Disease Models 4.0 via automation and high-throughput processing.
Biofabrication, 2019Co-Authors: Sebastian Eggert, Dietmar W. HutmacherAbstract:While much progress has been accomplished in the development of physiologically relevant in vitro Disease Models, current manufacturing and characterisation workflows still rely on manual, time-consuming, and low-throughput processes, which are not efficient and prone to human errors. For these reasons adoption and, more importantly, reproducibility and validation of 3D in vitro Disease Models is rather low for fundamental and applied research concepts. This article argues in form of a perspective view that automation and high-throughput methodologies will play a vital role to act as a catalyst to accelerate the development and characterisation process for generations to come. Innovative engineering concepts are required to overcome current limitations of in vitro Disease Models and to foster the scientific rigour as well as the applied research potential.
Heikki Tanila - One of the best experts on this subject based on the ideXlab platform.
-
Testing cognitive functions in rodent Disease Models: Present pitfalls and future perspectives.
Behavioural brain research, 2017Co-Authors: Heikki TanilaAbstract:Testing of cognitive functions in rodent Disease Models constitutes a substantial sector of behavioral neuroscience. It is most often needed in phenotyping genetically modified new rodent (usually mouse) lines or in preclinical testing of cognitive effects of new CNS drugs. This review concerns present pitfalls and future perspectives in this large field, with an emphasis on memory testing in CNS Disease Models and their preclinical drug testing. It is important to realize that no behavioral test is specific for a single cognitive domain. There are numerous noncognitive factors that may lead to impaired performance in most widely applied memory tasks. It is important to rule these out by applying a battery of test that should include at least tests for motor functions, spontaneous activity and anxiety besides cognitive aspects. In addition, considering and reporting all task-relavant details will help to resolve the common problem that certain behavioral findings cannot be reproduced by other laboratories. More collaboration between molecular and behavioral neuroscience laboratories and systematic training of young neuroscientist on behavioral techniques will help ensure quality of behavioral studies in the future.