The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Tokuzo Fujimoto - One of the best experts on this subject based on the ideXlab platform.
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successful treatment of chronic disseminated intravascular coagulation using recombinant human soluble thrombomodulin in a dialysis patient with Dissecting Aortic Aneurysm
The Japanese journal of clinical hematology, 2014Co-Authors: Kana Hayakawa, Shinobu Tamura, Hiroya Gima, Takahiro Hayakawa, Toshio Kurihara, Maki Ooura, Yoshio Nakano, Masayoshi Souri, Akitada Ichinose, Tokuzo FujimotoAbstract:Abstract A 62-year-old man had a history of acute Aortic dissection (Stanford type A) and had been diagnosed with polycystic kidney disease three years earlier, and then developed end-stage renal failure. He was referred with chief complaints of difficult hemostasis and consecutive hemorrhagic episodes at the puncture site of the shunt soon after dialysis introduction. We suspected chronic disseminated intravascular coagulation (DIC) due to mild thrombocytopenia and a fibrinolytic system abnormality. Plasma factor XIII activity was decreased, but no inhibitor was detected. In addition, contrast-enhanced computed tomography showed exacerbation of a Dissecting Aortic Aneurysm. We finally diagnosed chronic DIC and secondary factor XIII deficiency associated with the Aortic Aneurysm. We selected treatment involving recombinant human soluble thrombomodulin (rTM) because he was on maintenance dialysis and required long-term follow-up bofore the operation. Hemostatic function improved with regular administration of rTM, and was well-controlled preoperatively.
Paul J Boor - One of the best experts on this subject based on the ideXlab platform.
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n 2 aminoethyl ethanolamine induced morphological biochemical and biophysical alterations in vascular matrix associated with Dissecting Aortic Aneurysm
Toxicological Sciences, 2015Co-Authors: Zhenping Chen, Ya Xu, Paul J Bujalowski, Andres F Oberhauser, Paul J BoorAbstract:Dissecting Aortic Aneurysm (DAA) is an extended tear in the wall of the aorta along the plane of the vascular media. Our previous studies indicated in a developmental animal model, that DAA was related to pathological alteration in collagen, especially collagen type III. Accordingly, in the present studies, neonatal Aortic vascular smooth muscle cells (VSMC) and timed pregnant Sprague-Dawley rat dams were treated with N-(2-aminoethyl) ethanolamine (AEEA), which, as shown previously, causes DAA in offspring. Morphological changes in extracellular matrix (ECM) produced by VSMC in vitro were detailed with scanning electron microscopy (SEM), and biochemical changes in cells and ECM produced by VSMCs were defined by Western blotting. Biophysical changes of the collagen extracted from both the ECM produced by VSMC and extracted from fetal rat aortas were studied with atomic force microscopy (AFM). ECM disruption and irregularities were observed in VSMCs treated with AEEA by SEM. Western blotting showed that collagen type I was much more extractable, accompanied by a decrease of the pellet size after urea buffer extraction in the AEEA-treated VSMC when compared with the control. AFM found that collagen samples extracted from the fetal rat aortas of the AEEA-treated dam, and in the in vitro formed ECM prepared by decellularization, became stiffer, or more brittle, indicating that the 3D organization associated with elasticity was altered by AEEA exposure. Our results show that AEEA causes significant morphological, biochemical, and biomechanical alterations in the ECM. These in vitro and in vivo strategies are advantageous in elucidating the underlying mechanisms of DAA.
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Dissecting Aortic Aneurysm induced by n 2 aminoethyl ethanolamine in rat role of defective collagen during development
Birth Defects Research Part A-clinical and Molecular Teratology, 2014Co-Authors: Ya Xu, Silke Treumann, Roland Rossbacher, Steffen Schneider, Paul J BoorAbstract:Background Dissecting Aortic Aneurysm (DAA) is a tear in the wall of the aorta that causes blood to flow, or “dissect,” between the medial layers of the media. Methods Pregnant rats (dams) were treated with the industrial chemical n-(2-aminoethyl) ethanolamine (AEEA) by intraperitoneal injection or gavage. The histology and pathology of aorta in the thorax from newborn pups were examined. Aortas of fetuses of gestational day 20 from dams exposed to AEEA were harvested for immunohistochemical staining and native Western blot to study the changes of collagen type 1 and type 3 in aorta. Results Dissecting Aortic Aneurysm of newborn rats was induced by treating with AEEA through intraperitoneal injection or gavage. The incidence of DAA reached 100% in live pups at the high dose by means of gavage of AEEA, but without lethality compared with intraperitoneal injection. A grading system for the dose-response of DAA lesions associated with AEEA by gavage was established. Gestational day 20 fetuses from treated dams showed a decreased content and altered distribution of medial and adventitial collagen type 1 and 3 in aorta by immunohistochemistry; this decrease was confirmed by native Western blot. Conclusion This in vivo model of spontaneous Aortic dissection bears striking similarities histologically to human Aortic dissection. As such, the model conceivably could contribute to elucidating the mechanisms of DAA formation and to exploring diagnostic and therapeutic strategies. The pathogenesis of AEEA-induced DAA may be related to defects in the normal developmental progression of collagen types 1 and 3 in the vascular wall. Birth Defects Research (Part A), 2014. © 2014 Wiley Periodicals, Inc. Birth Defects Research (Part A) 100:924–933, 2014. © 2014 Wiley Periodicals, Inc.
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nonlinear imaging study of extracellular matrix in chemical induced developmental Dissecting Aortic Aneurysm evidence for defective collagen type iii
Birth Defects Research Part A-clinical and Molecular Teratology, 2008Co-Authors: Bin Gong, Ya Xu, Deepak Srivastava, Gracie Vargas, Qing Chang, Paul J BoorAbstract:BACKGROUND: Using a recent model of Dissecting Aortic Aneurysm (DAA) caused by in utero exposure to semicarbazide, we examined the elastin and collagen using standard methods and two nonlinear imaging techniques, multiphoton fluorescence (MPF) and second harmonic generation (SHG) microscopy. METHODS: Sprague-Dawley rat dams were given semicarbazide (6.13 mg/kg/day i.p.) from gestational days 14 to 20 (GD14–20). Fetuses were harvested on GD20 and pups on postnatal day 1 (PND1), PND7, and PND28; matched controls were from dams treated with saline. Aortic immunohistopathology and collagen/elastin signal intensity via MPF and SHG microscopy at an excitation wavelength of 800 nm were studied. RESULTS: Massive DAA of the Aortic arch occurred in nearly 100% of pups at birth (i.e., no GD20 fetuses showed lesions). MPF and SHG demonstrated that collagen was significantly degraded at GD20 and in newborns, but normalized by PND28. GD20 fetuses and newborn pups showed a decreased content of medial and adventitial collagen type III in pooled aortas by Western blot and immunohistochemistry. In 7- and 28-day-old pups resolution of DAA blood in vascular media and a recovery of stainable collagen type III was found. Elastin in healed DAA (PND28 pups) was focally disorganized. CONCLUSION: MPF and SHG microscopy provide sensitive and high-resolution information on Aortic elastin and collagen. In this model of DAA, collagen displays aberrant imaging quality likely linked to a marked decrease in collagen type III in the developing extracellular matrix. Birth Defects Research (Part A) 2008. © 2007 Wiley-Liss, Inc.
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chemical induced nonlethal developmental model of Dissecting Aortic Aneurysm
Birth Defects Research Part A-clinical and Molecular Teratology, 2006Co-Authors: Bin Gong, M B Trent, Deepak Srivastava, Paul J BoorAbstract:BACKGROUND: A chemical-induced, nonlethal, Dissecting Aortic Aneurysm (DAA) is described following in utero exposure to semicarbazide, an inhibitor of the vascular enzyme semicarbazide sensitive amine oxidase (SSAO). METHODS: Sprague-Dawley rat dams were given semicarbazide (0.096 – 49.000 mg/kg/day) by IP injection on gestation days (GDs) 14 –20, a period of rapid Aortic development. Newborn rats (day 1) were killed and their thoracic organs were removed en bloc for near-serial cross sections and routine histopathology, Movat stain for elastin, and immunohistochemistry to differentiate cells involved in the evolution of the DAA. In subsequent experiments, pups from treated dams (0.096 – 6.125 mg/kg/day) were allowed to survive for 7 or 28 days. RESULTS: DAA occurred in nearly 100% of the rats at all doses except the lowest tested (1.530, 0.096 mg/kg/day). Dissections frequently extended to the carotids and, less frequently, to the abdominal aorta. Remodeling of vascular lesions proceeded by organization of collections of blood in vascular media (the “false lumen”), proliferation of vascular smooth muscle cells, fibrosis, and formation of irregular frayed elastic lamellae in healed vascular media. Biochemical quantitation and Western blot analysis of main extracellular matrix proteins on GD 20 showed no overt difference in expression of collagen type I, fibrillin-1, or elastin. CONCLUSION: This developmental model provides investigators an opportunity to explore the pathologic mechanisms of DAA and to examine the potential long-term effects of vascular remodeling of DAA. Birth Defects Research (Part A) 76:29 –38, 2006. © 2006 Wiley-Liss, Inc.
Kana Hayakawa - One of the best experts on this subject based on the ideXlab platform.
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successful treatment of chronic disseminated intravascular coagulation using recombinant human soluble thrombomodulin in a dialysis patient with Dissecting Aortic Aneurysm
The Japanese journal of clinical hematology, 2014Co-Authors: Kana Hayakawa, Shinobu Tamura, Hiroya Gima, Takahiro Hayakawa, Toshio Kurihara, Maki Ooura, Yoshio Nakano, Masayoshi Souri, Akitada Ichinose, Tokuzo FujimotoAbstract:Abstract A 62-year-old man had a history of acute Aortic dissection (Stanford type A) and had been diagnosed with polycystic kidney disease three years earlier, and then developed end-stage renal failure. He was referred with chief complaints of difficult hemostasis and consecutive hemorrhagic episodes at the puncture site of the shunt soon after dialysis introduction. We suspected chronic disseminated intravascular coagulation (DIC) due to mild thrombocytopenia and a fibrinolytic system abnormality. Plasma factor XIII activity was decreased, but no inhibitor was detected. In addition, contrast-enhanced computed tomography showed exacerbation of a Dissecting Aortic Aneurysm. We finally diagnosed chronic DIC and secondary factor XIII deficiency associated with the Aortic Aneurysm. We selected treatment involving recombinant human soluble thrombomodulin (rTM) because he was on maintenance dialysis and required long-term follow-up bofore the operation. Hemostatic function improved with regular administration of rTM, and was well-controlled preoperatively.
Shuichiro Takanashi - One of the best experts on this subject based on the ideXlab platform.
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superior vena cava syndrome secondary to chronic Dissecting Aortic Aneurysm after Aortic valve replacement
Interactive Cardiovascular and Thoracic Surgery, 2010Co-Authors: Toshihiro Fukui, Daijun Ro, Shuichiro TakanashiAbstract:Ascending Aortic Aneurysm is a rare cause of superior vena cava syndrome. Herein, we describe a case of superior vena cava syndrome caused by a chronic Dissecting Aortic Aneurysm after Aortic valve replacement. A successful replacement of the Aortic root and ascending aorta led to an improvement of edema of the face and bilateral upper limbs caused by superior vena cava syndrome.
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Superior vena cava syndrome secondary to chronic Dissecting Aortic Aneurysm after Aortic valve replacement
Interactive Cardiovascular and Thoracic Surgery, 2010Co-Authors: Toshihiro Fukui, Daijun Ro, Shuichiro TakanashiAbstract:Ascending Aortic Aneurysm is a rare cause of superior vena cava syndrome. Herein, we describe a case of superior vena cava syndrome caused by a chronic Dissecting Aortic Aneurysm after Aortic valve replacement. A successful replacement of the Aortic root and ascending aorta led to an improvement of edema of the face and bilateral upper limbs caused by superior vena cava syndrome. 2010 Published by European Association for Cardio-Thoracic Surgery. All rights reserved.
Hiroya Gima - One of the best experts on this subject based on the ideXlab platform.
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successful treatment of chronic disseminated intravascular coagulation using recombinant human soluble thrombomodulin in a dialysis patient with Dissecting Aortic Aneurysm
The Japanese journal of clinical hematology, 2014Co-Authors: Kana Hayakawa, Shinobu Tamura, Hiroya Gima, Takahiro Hayakawa, Toshio Kurihara, Maki Ooura, Yoshio Nakano, Masayoshi Souri, Akitada Ichinose, Tokuzo FujimotoAbstract:Abstract A 62-year-old man had a history of acute Aortic dissection (Stanford type A) and had been diagnosed with polycystic kidney disease three years earlier, and then developed end-stage renal failure. He was referred with chief complaints of difficult hemostasis and consecutive hemorrhagic episodes at the puncture site of the shunt soon after dialysis introduction. We suspected chronic disseminated intravascular coagulation (DIC) due to mild thrombocytopenia and a fibrinolytic system abnormality. Plasma factor XIII activity was decreased, but no inhibitor was detected. In addition, contrast-enhanced computed tomography showed exacerbation of a Dissecting Aortic Aneurysm. We finally diagnosed chronic DIC and secondary factor XIII deficiency associated with the Aortic Aneurysm. We selected treatment involving recombinant human soluble thrombomodulin (rTM) because he was on maintenance dialysis and required long-term follow-up bofore the operation. Hemostatic function improved with regular administration of rTM, and was well-controlled preoperatively.