The Experts below are selected from a list of 588 Experts worldwide ranked by ideXlab platform

H U Shouwang - One of the best experts on this subject based on the ideXlab platform.

  • the preparation and application evaluation of DNA Microarray genechip Kit for hiv detection
    Journal of Molecular Diagnostics and Therapy, 2010
    Co-Authors: H U Shouwang
    Abstract:

    Objective To develop a DNA Microarray Kit for HIV detection,and to evaluate the application value in clinical diagnosis and entry-exit inspection and quarantine.Methods The specific oligonucleotide probes for HIV-1 gags region and interior label or comparison probes were designed and spotted on glass slides using a Microarray spotter.DNA sample were by amplified asymmetric PCR for HIV gags gene and the products of PCR were labeled with the fluorescent dye Cy3.The asymmetric PCR primer concentration ratio was 1:20.Following hybridization between the Cy3-labeled PCR products and the oligonucleotide probes of each Microarray on the slides and wash, the amount of probe hybridizing to each DNA sample was measured with a Microarray scanner and the image was analyzed with a computer software, which was used to identify the positive probes and to determine the HIV on each sample.Results There was a good concordance between the genotyping results by oligonucleotide Microarray and those of SBT.70 samples were tested and the coincidence rate was 98.57% .Conclusion The procedure described here has the potential of providing a rapid, simple, economical and accurate way for HIV detection, which has high application value in clinical diagnosis and entry-exit inspection and quarantine.

Albert Eisenbarth - One of the best experts on this subject based on the ideXlab platform.

  • Development of a Low-Density DNA Microarray for Detecting Tick-Borne Bacterial and Piroplasmid Pathogens in African Cattle
    'MDPI AG', 2019
    Co-Authors: Babette Abanda, Archile Paguem, Mbunkah Daniel Achukwi, Alfons Renz, Albert Eisenbarth
    Abstract:

    In Africa, pathogens transmitted by ticks are of major concern in livestock production and human health. Despite noticeable improvements particularly of molecular screening methods, their widespread availability and the detection of multiple infections remain challenging. Hence, we developed a universally accessible and robust tool for the detection of bacterial pathogens and piroplasmid parasites of cattle. A low-cost and low-density chip DNA Microarray Kit (LCD-Array) was designed and tested towards its specificity and sensitivity for five genera causing tick-borne diseases. The blood samples used for this study were collected from cattle in Northern Cameroon. Altogether, 12 species of the genera Anaplasma, Ehrlichia, Rickettsia and Theileria, and their corresponding genus-wide probes including Babesia were tested on a single LCD-Array. The detection limit of plasmid controls by PCR ranged from 1 to 75 copies per µL depending on the species. All sequenced species hybridized on the LCD-Array. As expected, PCR, agarose gel electrophoresis and Sanger sequencing found significantly less pathogens than the LCD-Array (p < 0.001). Theileria and Rickettsia had lower detection limits than Anaplasma and Ehrlichia. The parallel identification of some of the most detrimental tick-borne pathogens of livestock, and the possible implementation in small molecular-diagnostic laboratories with limited capacities makes the LCD-Array an appealing asset

Babette Abanda - One of the best experts on this subject based on the ideXlab platform.

  • Development of a Low-Density DNA Microarray for Detecting Tick-Borne Bacterial and Piroplasmid Pathogens in African Cattle
    'MDPI AG', 2019
    Co-Authors: Babette Abanda, Archile Paguem, Mbunkah Daniel Achukwi, Alfons Renz, Albert Eisenbarth
    Abstract:

    In Africa, pathogens transmitted by ticks are of major concern in livestock production and human health. Despite noticeable improvements particularly of molecular screening methods, their widespread availability and the detection of multiple infections remain challenging. Hence, we developed a universally accessible and robust tool for the detection of bacterial pathogens and piroplasmid parasites of cattle. A low-cost and low-density chip DNA Microarray Kit (LCD-Array) was designed and tested towards its specificity and sensitivity for five genera causing tick-borne diseases. The blood samples used for this study were collected from cattle in Northern Cameroon. Altogether, 12 species of the genera Anaplasma, Ehrlichia, Rickettsia and Theileria, and their corresponding genus-wide probes including Babesia were tested on a single LCD-Array. The detection limit of plasmid controls by PCR ranged from 1 to 75 copies per µL depending on the species. All sequenced species hybridized on the LCD-Array. As expected, PCR, agarose gel electrophoresis and Sanger sequencing found significantly less pathogens than the LCD-Array (p < 0.001). Theileria and Rickettsia had lower detection limits than Anaplasma and Ehrlichia. The parallel identification of some of the most detrimental tick-borne pathogens of livestock, and the possible implementation in small molecular-diagnostic laboratories with limited capacities makes the LCD-Array an appealing asset

Archile Paguem - One of the best experts on this subject based on the ideXlab platform.

  • Development of a Low-Density DNA Microarray for Detecting Tick-Borne Bacterial and Piroplasmid Pathogens in African Cattle
    'MDPI AG', 2019
    Co-Authors: Babette Abanda, Archile Paguem, Mbunkah Daniel Achukwi, Alfons Renz, Albert Eisenbarth
    Abstract:

    In Africa, pathogens transmitted by ticks are of major concern in livestock production and human health. Despite noticeable improvements particularly of molecular screening methods, their widespread availability and the detection of multiple infections remain challenging. Hence, we developed a universally accessible and robust tool for the detection of bacterial pathogens and piroplasmid parasites of cattle. A low-cost and low-density chip DNA Microarray Kit (LCD-Array) was designed and tested towards its specificity and sensitivity for five genera causing tick-borne diseases. The blood samples used for this study were collected from cattle in Northern Cameroon. Altogether, 12 species of the genera Anaplasma, Ehrlichia, Rickettsia and Theileria, and their corresponding genus-wide probes including Babesia were tested on a single LCD-Array. The detection limit of plasmid controls by PCR ranged from 1 to 75 copies per µL depending on the species. All sequenced species hybridized on the LCD-Array. As expected, PCR, agarose gel electrophoresis and Sanger sequencing found significantly less pathogens than the LCD-Array (p < 0.001). Theileria and Rickettsia had lower detection limits than Anaplasma and Ehrlichia. The parallel identification of some of the most detrimental tick-borne pathogens of livestock, and the possible implementation in small molecular-diagnostic laboratories with limited capacities makes the LCD-Array an appealing asset

Mbunkah Daniel Achukwi - One of the best experts on this subject based on the ideXlab platform.

  • Development of a Low-Density DNA Microarray for Detecting Tick-Borne Bacterial and Piroplasmid Pathogens in African Cattle
    'MDPI AG', 2019
    Co-Authors: Babette Abanda, Archile Paguem, Mbunkah Daniel Achukwi, Alfons Renz, Albert Eisenbarth
    Abstract:

    In Africa, pathogens transmitted by ticks are of major concern in livestock production and human health. Despite noticeable improvements particularly of molecular screening methods, their widespread availability and the detection of multiple infections remain challenging. Hence, we developed a universally accessible and robust tool for the detection of bacterial pathogens and piroplasmid parasites of cattle. A low-cost and low-density chip DNA Microarray Kit (LCD-Array) was designed and tested towards its specificity and sensitivity for five genera causing tick-borne diseases. The blood samples used for this study were collected from cattle in Northern Cameroon. Altogether, 12 species of the genera Anaplasma, Ehrlichia, Rickettsia and Theileria, and their corresponding genus-wide probes including Babesia were tested on a single LCD-Array. The detection limit of plasmid controls by PCR ranged from 1 to 75 copies per µL depending on the species. All sequenced species hybridized on the LCD-Array. As expected, PCR, agarose gel electrophoresis and Sanger sequencing found significantly less pathogens than the LCD-Array (p < 0.001). Theileria and Rickettsia had lower detection limits than Anaplasma and Ehrlichia. The parallel identification of some of the most detrimental tick-borne pathogens of livestock, and the possible implementation in small molecular-diagnostic laboratories with limited capacities makes the LCD-Array an appealing asset