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Filip Claes - One of the best experts on this subject based on the ideXlab platform.

  • Genome-Wide SNP Analysis Reveals Distinct Origins of Trypanosoma evansi and Trypanosoma equiperdum.
    Genome Biology and Evolution, 2017
    Co-Authors: Bart Cuypers, Nick Van Reet, Julien Cauchard, Filip Claes, Bruno Goddeeris, Frederik Van Den Broeck, Conor J. Meehan, Jonathan M. Wilkes, Hadush Birhanu, Jean-claude Dujardin
    Abstract:

    Trypanosomes cause a variety of diseases in man and domestic animals in Africa, Latin America, and Asia. In the Trypanozoon subgenus, Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense cause human African trypanosomiasis, whereas Trypanosoma brucei brucei, Trypanosoma evansi, and Trypanosoma equiperdum are responsible for nagana, surra, and Dourine in domestic animals, respectively. The genetic relationships between T. evansi and T. equiperdum and other Trypanozoon species remain unclear because the majority of phylogenetic analyses has been based on only a few genes. In this study, we have conducted a phylogenetic analysis based on genome-wide SNP analysis comprising 56 genomes from the Trypanozoon subgenus. Our data reveal that T. equiperdum has emerged at least once in Eastern Africa and T. evansi at two independent occasions in Western Africa. The genomes within the T. equiperdum and T. evansi monophyletic clusters show extremely little variation, probably due to the clonal spread linked to the independence from tsetse flies for their transmission.

  • Comparative diagnosis of parasitological, serological, and molecular tests in Dourine-suspected horses
    Tropical Animal Health and Production, 2010
    Co-Authors: Fikru Regassa Gari, Hagos Ashenafi, Bruno Goddeeris, Alemu Tola, Filip Claes
    Abstract:

    Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two Dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/ Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from Dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that Dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference ( P  > 0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference ( P  

  • efficacy of cymelarsan and diminasan against trypanosoma equiperdum infections in mice and horses
    Veterinary Parasitology, 2010
    Co-Authors: A Hagos, Bruno Goddeeris, K Yilkal, T Alemu, Regassa Fikru, H Yacob, G Feseha, Filip Claes
    Abstract:

    Trypanocidal sensitivity studies were conducted to assess the efficacy of Diminazene diaceturate (Diminasan®) and Bis (aminoethylthio) 4-melaminophenylarsine dihydrochloride (Cymelarsan®) against Trypanosoma equiperdum (isolated from two mares with chronic cases of Dourine) 713/943 and 834/940 Dodola strains in experimentally infected mice and horses. Diminasan® at doses from 3.5 mg/kg to 28 mg/kg and Cymelarsan® at doses of 0.25 mg/kg and 0.5 mg/kg body weight failed to cure any of the mice, indicating a clear dose dependent relationship in the mean time of relapse observed in mice. Indeed, mice treated with lower doses relapsed after a shorter time than mice treated with higher doses. However, mice treated with Cymelarsan® at doses of 1.0 mg/kg and 2.0 mg/kg body weight were cured and no parasitemia was observed for 60 days. The efficacy of Cymelarsan® was also tested in horses. Two groups of horses containing two animals each were infected with T. equiperdum 834/940 Dodola strain and treated with Cymelarsan® at a dose rate of 0.25 mg/kg and 0.5 mg/kg, respectively. Cymelarsan® at 0.25 mg/kg and 0.5 mg/kg body weight cleared parasitemia within 24 h post treatment and none of the animals were found to show relapse throughout the 320 days of observation. The sensitivity of the particular trypanosome strain to Cymelarsan® was also supported by the relative improvement in the mean PCV levels of horses following treatment. A statistically significant difference (p < 0.01) in the mean PCV levels of horses treated with Cymelarsan® was observed between day 20 at peak parasitemia and days 40 as well as 60 of observation. The mean PCV levels of horses in the control group progressively decreased within the first 60 days of post infection. Two of the horses in the control group developed chronic form of Dourine manifested by genital as well as nervous signs with progressive loss of body condition within 320 days post infection. The efficacy of Cymelarsan® against the chronic form of Dourine was confirmed after treatment of one of the control horses with Cymelarsan® at a dose rate of 0.25 mg/kg body weight at day 282 post infection. It was noted that the treated horse improved overall body condition and clinical signs such as incoordination of hind legs, weakness and ventral oedema disappeared within 10 days of treatment. Thus, Cymelarsan® was found to be quite effective in curing horses in acute as well as chronic form of Dourine. The results obtained from the present study will be important for designing effective control measures against Dourine.

  • Serological and parasitological survey of Dourine in the Arsi-Bale highlands of Ethiopia.
    Tropical animal health and production, 2009
    Co-Authors: Ashenafi Hagos, Bruno Goddeeris, Getachew Abebe, Philip Büscher, Filip Claes
    Abstract:

    This study was conducted from August 2005 to January 2007 to determine prevalence and distribution of Dourine in horses and to investigate the occurrence of clinical and carrier cases in donkeys and mules in the Arsi-Bale highlands. Study methodology was based on questionnaire, serological, clinical and parasitological survey. The questionnaire indicated that Dourine is a major health problem of equines in the Arsi-Bale highlands. Though Dourine is commonly observed throughout the year, it has a seasonal character and occurs mostly during the breeding season from June to late September. Serological screening of 646 horses showed a seroprevalence of 184 (28%), 161 (25%) and 125 (19%) for card agglutination test for trypanosomosis, LATEX and enzyme-linked immunosorbent assay, respectively. Risk factors were parity number, previous history of abortion and body condition score. No trypanosomes could be detected by Giemsa staining or by haematocrit centrifugation technique. Ten puppies inoculated with blood samples, genital washes and oedematous fluids remained parasitologically negative. Different characteristic signs of Dourine were observed. During the genital stage, mares showed vaginal oedema, discharge and presence of depigmented scars over the external genitalia. In stallions, oedema of the scrotum and prepuce, prepucial and urethral discharge, and ulceration of the genital mucosae mainly of the penile were observed. In both sexes, lameness in one or both legs, partial dragging and stiffness of the hind legs and incoordination were the dominant signs observed as nervous form of the disease.

  • Trypanosoma equiperdum: master of disguise or historical mistake?
    Trends in Parasitology, 2005
    Co-Authors: Filip Claes, Philippe Büscher, Louis Touratier, Bruno Goddeeris
    Abstract:

    After 100 years of research, only a small number of laboratory strains of Trypanosoma equiperdum exists, and the history of most of the strains is unknown. No definitive diagnosis of Dourine can be made at the serological or molecular level. Only clinical signs are pathognomonic and international screening relies on an outdated cross-reactive serological test (the complement-fixation test) from 1915, resulting in serious consequences at the practical level. Despite many characterization attempts, no clear picture has emerged of the position of T. equiperdum within the Trypanozoon group. In this article, we highlight the controversies that exist regarding T. equiperdum, and the overlap that occurs with Trypanosoma evansi and Trypanosoma brucei brucei. By revisiting the published data, from the early decades of discovery to the recent serological- and molecular-characterization studies, a new hypothesis arises in which T. equiperdum no longer exists as a separate species and in which current strains can be divided into T. evansi (the historical mistake) and Trypanosoma brucei equiperdum (the master of disguise). Hence, Dourine is a disease caused by specific host immune responses to a T. b. equiperdum or T. evansi infection.

Bruno Goddeeris - One of the best experts on this subject based on the ideXlab platform.

  • Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum
    BMC Veterinary Research, 2019
    Co-Authors: Ahmed Yasine, Hagos Ashenafi, Peter Geldhof, Merga Bekana, Alemu Tola, Griet Vercauteren, Leen Van Brantegem, Ann Van Soom, Luc Duchateau, Bruno Goddeeris
    Abstract:

    Background Dourine, a venereal transmitted trypanosomosis caused by Trypanosoma equiperdum , has different clinical signs related to the reproductive and nervous system. Pathologic tissue changes associated with the disease are poorly described. The present study describes the histopathological lesions in naturally T. equiperdum -infected horses in the chronical stage of Dourine. Results Four chronically Dourine diseased horses underwent a post-mortem examination. They were Woo test negative, but CATT /T. evansi positive, had a low packed cell volume (PCV) and exhibited obvious clinical signs of Dourine. Post-mortem examination did not reveal gross lesions in the organs assumed to be responsible for the symptomatology. On histopathology, genital organs were affected, with mononuclear cell infiltration and erosions and degeneration of seminiferous tubules and perivascular lymphoplasmacytic cuffing in the uterus. In the nervous system, mononuclear cell infiltration was located in peripheral nerves, ganglia and in the spinal cord, leading to axonal degeneration. Real-time PCR using ITS primer revealed the presence of trypanosomes in these organs and conventional PCRs using maxicircle and RoTat1.2 primers further confirmed the involvement of T. equiperdum since the DNAs from the vagina, testicle, distal spinal cord, sciatic and obturator nerves found to be positive for maxicircle and negative for RoTat 1.2. Conclusions The histopathological lesions in the spinal cord and peripheral nerves explain the incoordination of the hind legs in T. equiperdum -infected horses, whilst its presence in the genital tract exemplifies the venereal transmission.

  • Genome-Wide SNP Analysis Reveals Distinct Origins of Trypanosoma evansi and Trypanosoma equiperdum.
    Genome Biology and Evolution, 2017
    Co-Authors: Bart Cuypers, Nick Van Reet, Julien Cauchard, Filip Claes, Bruno Goddeeris, Frederik Van Den Broeck, Conor J. Meehan, Jonathan M. Wilkes, Hadush Birhanu, Jean-claude Dujardin
    Abstract:

    Trypanosomes cause a variety of diseases in man and domestic animals in Africa, Latin America, and Asia. In the Trypanozoon subgenus, Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense cause human African trypanosomiasis, whereas Trypanosoma brucei brucei, Trypanosoma evansi, and Trypanosoma equiperdum are responsible for nagana, surra, and Dourine in domestic animals, respectively. The genetic relationships between T. evansi and T. equiperdum and other Trypanozoon species remain unclear because the majority of phylogenetic analyses has been based on only a few genes. In this study, we have conducted a phylogenetic analysis based on genome-wide SNP analysis comprising 56 genomes from the Trypanozoon subgenus. Our data reveal that T. equiperdum has emerged at least once in Eastern Africa and T. evansi at two independent occasions in Western Africa. The genomes within the T. equiperdum and T. evansi monophyletic clusters show extremely little variation, probably due to the clonal spread linked to the independence from tsetse flies for their transmission.

  • Comparative diagnosis of parasitological, serological, and molecular tests in Dourine-suspected horses
    Tropical Animal Health and Production, 2010
    Co-Authors: Fikru Regassa Gari, Hagos Ashenafi, Bruno Goddeeris, Alemu Tola, Filip Claes
    Abstract:

    Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two Dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/ Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from Dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that Dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference ( P  > 0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference ( P  

  • Serological and parasitological survey of Dourine in the Arsi-Bale highlands of Ethiopia.
    Tropical animal health and production, 2009
    Co-Authors: Ashenafi Hagos, Bruno Goddeeris, Getachew Abebe, Philip Büscher, Filip Claes
    Abstract:

    This study was conducted from August 2005 to January 2007 to determine prevalence and distribution of Dourine in horses and to investigate the occurrence of clinical and carrier cases in donkeys and mules in the Arsi-Bale highlands. Study methodology was based on questionnaire, serological, clinical and parasitological survey. The questionnaire indicated that Dourine is a major health problem of equines in the Arsi-Bale highlands. Though Dourine is commonly observed throughout the year, it has a seasonal character and occurs mostly during the breeding season from June to late September. Serological screening of 646 horses showed a seroprevalence of 184 (28%), 161 (25%) and 125 (19%) for card agglutination test for trypanosomosis, LATEX and enzyme-linked immunosorbent assay, respectively. Risk factors were parity number, previous history of abortion and body condition score. No trypanosomes could be detected by Giemsa staining or by haematocrit centrifugation technique. Ten puppies inoculated with blood samples, genital washes and oedematous fluids remained parasitologically negative. Different characteristic signs of Dourine were observed. During the genital stage, mares showed vaginal oedema, discharge and presence of depigmented scars over the external genitalia. In stallions, oedema of the scrotum and prepuce, prepucial and urethral discharge, and ulceration of the genital mucosae mainly of the penile were observed. In both sexes, lameness in one or both legs, partial dragging and stiffness of the hind legs and incoordination were the dominant signs observed as nervous form of the disease.

  • Trypanosoma equiperdum: master of disguise or historical mistake?
    Trends in Parasitology, 2005
    Co-Authors: Filip Claes, Philippe Büscher, Louis Touratier, Bruno Goddeeris
    Abstract:

    After 100 years of research, only a small number of laboratory strains of Trypanosoma equiperdum exists, and the history of most of the strains is unknown. No definitive diagnosis of Dourine can be made at the serological or molecular level. Only clinical signs are pathognomonic and international screening relies on an outdated cross-reactive serological test (the complement-fixation test) from 1915, resulting in serious consequences at the practical level. Despite many characterization attempts, no clear picture has emerged of the position of T. equiperdum within the Trypanozoon group. In this article, we highlight the controversies that exist regarding T. equiperdum, and the overlap that occurs with Trypanosoma evansi and Trypanosoma brucei brucei. By revisiting the published data, from the early decades of discovery to the recent serological- and molecular-characterization studies, a new hypothesis arises in which T. equiperdum no longer exists as a separate species and in which current strains can be divided into T. evansi (the historical mistake) and Trypanosoma brucei equiperdum (the master of disguise). Hence, Dourine is a disease caused by specific host immune responses to a T. b. equiperdum or T. evansi infection.

Dosseh E.d. - One of the best experts on this subject based on the ideXlab platform.

  • Prise en charge de la retention aigue d’urine au CHU Sylvanus Olympio de Lome
    'African Journals Online (AJOL)', 2017
    Co-Authors: Botcho G, Tengue K, Kpatcha T.m., Sewa E.v., Leloua E, Sikpa H.k., Anoukoum T, Dosseh E.d.
    Abstract:

    Objectif: Décrire les aspects épidémiologiques, diagnostiques et thérapeutiques des rétentions aiguës d’urines au CHU Sylvanus Olympio de Lomé.Patients et Méthodes: Il s’est agi d’une étude prospective, descriptive et analytique réalisée aux urgences chirurgicales du CHU Sylvanus Olympio portant sur les patients présentant une rétention aiguë d’urine du 1er Juin 2010 au 31 Mai 2011 soit une période de 01 an.Résultats: L’étude a porté sur 100 patients dont l’âge moyen était de 58,08 ± 18,6 ans avec des extrêmes de 15 ans et 100 ans. La sex-ratio était de 24 hommes pour une femme. Avant la survenue de la rétention, 55 % ont eu une dysurie, 12 % un antécédent d’urétrite traitée et 4 % un antécédent de traumatisme de l’urètre. Les motifs d’admission étaient la douleur hypogastrique avec impossibilité d’uriner chez 90% des patients, le traumatisme du bassin chez 4% et le traumatisme du rachis chez 6% des patients. L’on notait chez 12% des patients, une issue de quelques gouttes d’urines. Les causes les plus fréquentes de la rétention aiguë d’urine étaient les affections prostatiques qui représentaient 75%. Pour le traitement initial, 62% des patients avaient bénéficié d’un cathétérisme suspubien et 38% d’un sondage transurétral puis 12 % ont présenté une hémorragie à vacuo après le drainage des urines. Le traitement définitif variait en fonction des différentes étiologies de la rétention aiguë d’urine.Conclusion: La rétention aiguë d’urine est la plus fréquente des urgences urologiques, mais il n’existe aucune donnée actuelle et réelle sur cette affection au CHU Sylvanus Olympio de Lomé. Le sexe masculin était le plus affecté, avec l’hypertrophie bénigne de la prostate comme la principale étiologie. Chez les femmes, la RAU était plus causée par le cancer de la vessie. Le traitement initial et définitif variait en fonction de l’étiologie, l’âge et le sexe.Mots clés: rétention aiguë d’urine, épidémiologie ; diagnostic ; traitement ; TogoEnglish Title: Management of acute urinary retention at the teaching hospital of Sylvanus Olympio in LomeEnglish AbstractPurpose: To describe the epidemiological, diagnostic and management aspects of acute urinary retention at the teaching hospital of Sylvanus Olympio in Lome.Patients and Methods: This is a prospective, descriptive, analytical study of the patients with acute urinary retention conducted in the surgicals emergencies of Sylvanus Olympio Teaching Hospital of Lome between 1st June 2010 and 31st May 2011.Results: There were 100 patients aged from 15 to 100 years old with an average of 58.08 ± 18.6 years. The sex ratio was 24. Before acute urinary retention, 55% of patients had  dysuria, 12% and 04% had respestively antecedent of urethritis and traumatism of urethra. The lower abdominal pain with inability to pass urine (90%), pelvic trauma (06%) and rachis trauma (04%) were the main reasons for consultation. Only 12% of patients could pass few drop of urine. The etiologies of acute urinary retention were mainly prostatic diseases (75%). The initial management of acute urinary retention was achieved via supra-pubic catheterisation (62%) and urethral catheter (38%) with 12% cases of hemorrhage post catheterisation. The definitive treatment for the underlying causes of acute urinary retention was varied.Conclusion: Acute urinary retention is the commonest urologic emergency, but there are no credible baseline data on this condition at the teaching hospital of Sylvanus Olympio, Lome. Men were affected more frequently, with benign prostatic enlargement being the leading cause. In the female patients, acute urinary retention was mainly caused by bladder cancer. Initial and definitive management varied, depending on the causes, age and gender.Keywords: Acute urinary retention ; epidemiology ; diagnosis ; management; Tog

  • Prise en charge de la retention aigue d’urine au Chu Sylvanus Olympio de Lome
    'African Journals Online (AJOL)', 2016
    Co-Authors: Botcho G, Tengue K, Kpatcha T.m., Sewa E.v., Leloua E, Sikpa H.k., Anoukoum T, Dosseh E.d.
    Abstract:

    Objectif: Décrire les aspects épidémiologiques, diagnostiques et thérapeutiques des rétentions aiguës d’urines au CHU Sylvanus Olympio de Lomé.Patients et Méthodes: Il s’est agi d’une étude prospective, descriptive et analytique réalisée aux urgences chirurgicales du CHU Sylvanus Olympio portant sur les patients présentant une rétention aiguë d’urine du 1er Juin 2010 au 31 Mai 2011 soit une période de 01 an.Résultats: L’étude a porté sur 100 patients dont l’âge moyen était de 58,08 ± 18,6 ans avec des extrêmes de 15 ans et 100 ans. La sex-ratio était de 24 hommes pour une femme. Avant la survenue de la rétention, 55 % ont eu une dysurie, 12% un antécédent d’urétrite traitée et 4% un antécédent de traumatisme de l’urètre. Les motifs d’admission étaient la douleur hypogastrique avec impossibilité d’uriner chez 90% des patients, le traumatisme du bassin chez 4% et le traumatisme du rachis chez 6% des patients. L’on notait chez 12 % des patients, une issue de quelques gouttes d’urines. Les causes les plus fréquentes de la rétention aiguë d’urine étaient les affections prostatiques qui représentaient 75%. Pour le traitement initial, 62% des patients avaient bénéficié d’un cathétérisme suspubien et 38% d’un sondage transurétral puis 12% ont présenté une hémorragie à vacuo après le drainage des urines. Le traitement définitif variait en fonction des différentes étiologies de la rétention aiguë d’urine.Conclusion: La rétention aiguë d’urine est la plus fréquente des urgences urologiques, mais il n’existe aucune donnée actuelle et réelle sur cette affection au CHU Sylvanus Olympio de Lomé. Le sexe masculin était le plus affecté, avec l’hypertrophie bénigne de la prostate comme la principale étiologie. Chez les femmes, la RAU était plus causée par le cancer de la vessie. Le traitement initial et définitif variait en fonction de l’étiologie, l’âge et le sexe.Mots clés: rétention aiguë d’urine, épidémiologie, diagnostic, traitement, TogoEnglish AbstractPurpose: To describe the epidemiological, diagnostic and management aspects of acute urinary retention at the teaching hospital of Sylvanus Olympio in Lome.Patients and Methods: This is a prospective, descriptive, analytical study of the patients with acute urinary retention conducted in the surgicals emergencies of Sylvanus Olympio Teaching Hospital of Lome between 1st June 2010 and 31st May 2011.Results: There were 100 patients aged from 15 to 100 years old with an average of 58.08 ± 18.6 years. The sex ratio was 24. Before acute urinary retention, 55% of patients had  dysuria, 12% and 04% had respectively antecedent of urethritis and traumatism of urethra. The lower abdominal pain with inability to pass urine (90%), pelvic trauma (06%) and rachis trauma (04%) were the main reasons for consultation. Only 12% of patients could pass few drop of urine. The etiologies of acute urinary retention were mainly prostatic diseases (75%). The initial management of acute urinary retention was achieved via supra-pubic catheterisation (62%) and urethral catheter (38%) with 12% cases of hemorrhage post catheterisation. The definitive treatment for the underlying causes of acute urinary retention was varied.Conclusion: Acute urinary retention is the commonest urologic emergency, but there are no credible baseline data on this condition at the teaching hospital of Sylvanus Olympio, Lome. Men were affected more frequently, with benign prostatic enlargement being the leading cause. In the female patients, acute urinary retention was mainly caused by bladder cancer. Initial and definitive management varied, depending on the causes, age and gender.Keywords: Acute urinary retention ; epidemiology ; diagnosis ; management; Tog

C Pfister - One of the best experts on this subject based on the ideXlab platform.

  • the orexin type 1 receptor is overexpressed in advanced prostate cancer with a neuroendocrine differentiation and mediates apoptosis
    European Journal of Cancer, 2014
    Co-Authors: David Alexandre, Coralie Hautot, Marwa Mehio, Lydie Jeandel, Maite Courel, Thierry Voisin, Alain Couvineau, Francoise Gobet, Jerome Leprince, C Pfister
    Abstract:

    Aim: In the present study, we have examined the presence of orexins and their receptors in prostate cancer (CaP) and investigated their effects on the apoptosis of prostate cancer cells. Methods: We have localised the orexin type 1 and 2 receptors (OX1R and OX2R) and orexin A (OxA) in CaP sections of various grades and we have quantified tumour cells containing OX1R. Expression of OX1R was evaluated in the androgeno-dependent (AD) LNCaP and the androgeno-independent (AI) DU145 prostate cancer cells submitted or not to a neuroen- docrine differentiation. The effects of orexins on the apoptosis and viability of DU145 cells were also investigated. Results: OX1R is strongly expressed in carcinomatous foci exhibiting a neuroendocrine differ- entiation, and the number of OX1R-stained cancer cells increases with the grade of the CaP. In contrast, OX2R is only detected in scattered malignant cells in high grade CaP. OX1R is

  • interet de l endoprothese uretrale dans la prise en charge d une retention aigue d urine apres curietherapie pour cancer localise de la prostate
    Progres En Urologie, 2014
    Co-Authors: W Kerkeni, C Chahwan, C Lenormand, B Dubray, A Benyoucef, C Pfister
    Abstract:

    Resume La curietherapie fait partie des options therapeutiques devant un cancer de prostate localise de faible risque. Bien qu’il s’agisse d’un traitement mini-invasif, il n’est pas denue d’effets secondaires. Une retention aigue d’urine apres curietherapie a ete rapportee dans 5 a 22 % des cas, elle peut etre prevenue dans la majorite des cas par un traitement alpha-bloquant. Plusieurs alternatives ont ete decrites dans la litterature pour la prise en charge de la retention d’urine apres curietherapie prostatique, parmi lesquelles le drainage prolonge par catheter sus-pubien, la resection trans-uretrale de prostate ou encore les autosondages intermittents. Nous rapportons un nouveau cas de retention d’urine dans les suites d’une curietherapie prostatique, prise en charge de facon originale par mise en place d’une endoprothese uretrale, permettant l’obtention d’un statut mictionnel satisfaisant.

Goddeeris Bruno - One of the best experts on this subject based on the ideXlab platform.

  • Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum
    'Springer Science and Business Media LLC', 2019
    Co-Authors: Yasine Ahmed, Van Soom Ann, Duchateau Luc, Ashenafi Hagos, Bekana Merga, Tola Alemu, Van Brantegem Leen, Geldhof Peter, Vercauteren Griet, Goddeeris Bruno
    Abstract:

    BACKGROUND: Dourine, a venereal transmitted trypanosomosis caused by Trypanosoma equiperdum, has different clinical signs related to the reproductive and nervous system. Pathologic tissue changes associated with the disease are poorly described. The present study describes the histopathological lesions in naturally T. equiperdum-infected horses in the chronical stage of Dourine. RESULTS: Four chronically Dourine diseased horses underwent a post-mortem examination. They were Woo test negative, but CATT/T. evansi positive, had a low packed cell volume (PCV) and exhibited obvious clinical signs of Dourine. Post-mortem examination did not reveal gross lesions in the organs assumed to be responsible for the symptomatology. On histopathology, genital organs were affected, with mononuclear cell infiltration and erosions and degeneration of seminiferous tubules and perivascular lymphoplasmacytic cuffing in the uterus. In the nervous system, mononuclear cell infiltration was located in peripheral nerves, ganglia and in the spinal cord, leading to axonal degeneration. Real-time PCR using ITS primer revealed the presence of trypanosomes in these organs and conventional PCRs using maxicircle and RoTat1.2 primers further confirmed the involvement of T. equiperdum since the DNAs from the vagina, testicle, distal spinal cord, sciatic and obturator nerves found to be positive for maxicircle and negative for RoTat 1.2. CONCLUSIONS: The histopathological lesions in the spinal cord and peripheral nerves explain the incoordination of the hind legs in T. equiperdum-infected horses, whilst its presence in the genital tract exemplifies the venereal transmission.status: publishe

  • Trypanosoma equiperdum in the horse : a neglected threat?
    2018
    Co-Authors: Ahmed Y, Hagos A, Merga B, Van Soom Ann, Duchateau Luc, Goddeeris Bruno, Govaere Jan
    Abstract:

    Dourine is a contagious disease caused by Trypanosoma equiperdum that is transmitted directly from animal to animal during coitus. Dourine is known as an important disease in many countries, and it threatens equidae worldwide. It is reported to be widespread in South America, Eastern Europe, Russia, Mongolia, Namibia and Ethiopia. The disease can be carried to various parts of the world through the transportation of infected animals and semen. Since knowledge of the prepatent infectiousness of a recently infected animal is lacking, introduction of the disease is in principle an ever-present threat. Definitive diagnosis depends on the identification of the parasite by means of direct microscopy. This is rarely possible in practice and therefore, diagnosis in the field is based on the observation of typical clinical signs, together with serological tests. This paper is an endeavour to review briefly and compile information on the appearance and importance of Dourine in terms of its epidemiological and clinical features, as well as on its diagnosis, treatment and prognosis

  • Genome-wide SNP analysis reveals distinct origins of Trypanosoma evansi and Trypanosoma equiperdum
    'Oxford University Press (OUP)', 2017
    Co-Authors: Cuypers Bart, Goddeeris Bruno, Claes Filip, Meehan, Conor J., Cauchard Julien, Van Den Broeck Frederik, Van Reet Nick, Wilkes, Jonathan M., Birhanu Hadush, Dujardin Jean-claude
    Abstract:

    Trypanosomes cause a variety of diseases in man and domestic animals in Africa, Latin America and Asia. In the Trypanozoon subgenus, Trypanosoma brucei gambiense and T. b. rhodesiense cause human African trypanosomiasis, while T. b. brucei, T. evansi and T. equiperdum are responsible for nagana, surra and Dourine in domestic animals, respectively. The genetic relationships between T. evansi and T. equiperdum and other Trypanozoon species remain unclear because the majority of phylogenetic analyses have been based on only a few genes. In this study, we have conducted a phylogenetic analysis based on genome-wide SNP analysis comprising 56 genomes from the Trypanozoon subgenus. Our data reveal that T. equiperdum has emerged at least once in Eastern Africa and T. evansi at two independent occasions in Western Africa. The genomes within the T. equiperdum and T. evansi monophyletic clusters show extremely little variation, probably due to the clonal spread linked to the independence from tsetse flies for their transmission. [Abstract copyright: © The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  • Efficacy of Cymelarsan and Diminasan against Trypanosoma equiperdum infections in mice and horses
    Elsevier Scientific Pub. Co., 2010
    Co-Authors: Hagos A, Goddeeris Bruno, Yilkal K, Alemu T, Fikru R, Yacob H T, Feseha G, Claes Filip
    Abstract:

    Trypanocidal sensitivity studies were conducted to assess the efficacy of Diminazene diaceturate (Diminasan) and Bis (aminoethylthio) 4-melaminophenylarsine dihydrochloride (Cymelarsan) against Trypanosoma equiperdum (isolated from two mares with chronic cases of Dourine) 713/943 and 834/940 Dodola strains in experimentally infected mice and horses. Diminasan at doses from 3.5 mg/kg to 28 mg/kg and Cymelarsan at doses of 0.25 mg/kg and 0.5 mg/kg body weight failed to cure any of the mice, indicating a clear dose dependent relationship in the mean time of relapse observed in mice. Indeed, mice treated with lower doses relapsed after a shorter time than mice treated with higher doses. However, mice treated with Cymelarsan at doses of 1.0 mg/kg and 2.0 mg/kg body weight were cured and no parasitemia was observed for 60 days. The efficacy of Cymelarsan was also tested in horses. Two groups of horses containing two animals each were infected with T. equiperdum 834/940 Dodola strain and treated with Cymelarsan at a dose rate of 0.25 mg/kg and 0.5 mg/kg, respectively. Cymelarsan at 0.25 mg/kg and 0.5 mg/kg body weight cleared parasitemia within 24 h post treatment and none of the animals were found to show relapse throughout the 320 days of observation. The sensitivity of the particular trypanosome strain to Cymelarsan was also supported by the relative improvement in the mean PCV levels of horses following treatment. A statistically significant difference (p

  • Serological and parasitological survey of Dourine in the Arsi-Bale highlands of Ethiopia
    'Springer Science and Business Media LLC', 2010
    Co-Authors: Hagos Ashenafi, Goddeeris Bruno, Abebe Getachew, Buscher Philip, Claes Filip
    Abstract:

    This study was conducted from August 2005 to January 2007 to determine prevalence and distribution of Dourine in horses and to investigate the occurrence of clinical and carrier cases in donkeys and mules in the Arsi-Bale highlands. Study methodology was based on questionnaire, serological, clinical and parasitological survey. The questionnaire indicated that Dourine is a major health problem of equines in the Arsi-Bale highlands. Though Dourine is commonly observed throughout the year, it has a seasonal character and occurs mostly during the breeding season from June to late September. Serological screening of 646 horses showed a seroprevalence of 184 (28%), 161 (25%) and 125 (19%) for card agglutination test for trypanosomosis, LATEX and enzyme-linked immunosorbent assay, respectively. Risk factors were parity number, previous history of abortion and body condition score. No trypanosomes could be detected by Giemsa staining or by haematocrit centrifugation technique. Ten puppies inoculated with blood samples, genital washes and oedematous fluids remained parasitologically negative. Different characteristic signs of Dourine were observed. During the genital stage, mares showed vaginal oedema, discharge and presence of depigmented scars over the external genitalia. In stallions, oedema of the scrotum and prepuce, prepucial and urethral discharge, and ulceration of the genital mucosae mainly of the penile were observed. In both sexes, lameness in one or both legs, partial dragging and stiffness of the hind legs and incoordination were the dominant signs observed as nervous form of the disease.status: publishe