The Experts below are selected from a list of 135681 Experts worldwide ranked by ideXlab platform
Hugo J. Bellen - One of the best experts on this subject based on the ideXlab platform.
-
Emerging technologies for gene manipulation in Drosophila Melanogaster.
Nature reviews. Genetics, 2005Co-Authors: Koen J. T. Venken, Hugo J. BellenAbstract:The popularity of Drosophila Melanogaster as a model for understanding eukaryotic biology over the past 100 years has been accompanied by the development of numerous tools for manipulating the fruitfly genome. Here we review some recent technologies that will allow Drosophila Melanogaster to be manipulated more easily than any other multicellular organism. These developments include the ability to create molecularly designed deletions, improved genetic mapping technologies, strategies for creating targeted mutations, new transgenic approaches and the means to clone and modify large fragments of DNA.
Uwe Karst - One of the best experts on this subject based on the ideXlab platform.
-
Quantitative Bioimaging to Investigate the Uptake of Mercury Species in Drosophila Melanogaster.
Analytical chemistry, 2015Co-Authors: Ann-christin Niehoff, Oliver Bolle Bauer, Sabrina Kröger, Stefanie Fingerhut, Jacqueline Schulz, Sören Meyer, Michael R. Sperling, Astrid Jeibmann, Tanja Schwerdtle, Uwe KarstAbstract:The uptake of mercury species in the model organism Drosophila Melanogaster was investigated by elemental bioimaging using laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS). The mercury distribution in Drosophila Melanogaster was analyzed for the three species mercury(II) chloride, methylmercury chloride, and thimerosal after intoxication. A respective analytical method was developed and applied to the analysis of the entire Drosophila Melanogaster first, before a particular focus was directed to the cerebral areas of larvae and adult flies. For quantification of mercury, matrix-matched standards based on gelatin were prepared. Challenges of spatially dissolved mercury determination, namely, strong evaporation issues of the analytes and an inhomogeneous distribution of mercury in the standards due to interactions with cysteine containing proteins of the gelatin were successfully addressed by complexation with meso-2,3-dimercaptosuccinic acid (DMSA). No mercury was detected in the cere...
Koen J. T. Venken - One of the best experts on this subject based on the ideXlab platform.
-
Emerging technologies for gene manipulation in Drosophila Melanogaster.
Nature reviews. Genetics, 2005Co-Authors: Koen J. T. Venken, Hugo J. BellenAbstract:The popularity of Drosophila Melanogaster as a model for understanding eukaryotic biology over the past 100 years has been accompanied by the development of numerous tools for manipulating the fruitfly genome. Here we review some recent technologies that will allow Drosophila Melanogaster to be manipulated more easily than any other multicellular organism. These developments include the ability to create molecularly designed deletions, improved genetic mapping technologies, strategies for creating targeted mutations, new transgenic approaches and the means to clone and modify large fragments of DNA.
Ann-christin Niehoff - One of the best experts on this subject based on the ideXlab platform.
-
Quantitative Bioimaging to Investigate the Uptake of Mercury Species in Drosophila Melanogaster.
Analytical chemistry, 2015Co-Authors: Ann-christin Niehoff, Oliver Bolle Bauer, Sabrina Kröger, Stefanie Fingerhut, Jacqueline Schulz, Sören Meyer, Michael R. Sperling, Astrid Jeibmann, Tanja Schwerdtle, Uwe KarstAbstract:The uptake of mercury species in the model organism Drosophila Melanogaster was investigated by elemental bioimaging using laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS). The mercury distribution in Drosophila Melanogaster was analyzed for the three species mercury(II) chloride, methylmercury chloride, and thimerosal after intoxication. A respective analytical method was developed and applied to the analysis of the entire Drosophila Melanogaster first, before a particular focus was directed to the cerebral areas of larvae and adult flies. For quantification of mercury, matrix-matched standards based on gelatin were prepared. Challenges of spatially dissolved mercury determination, namely, strong evaporation issues of the analytes and an inhomogeneous distribution of mercury in the standards due to interactions with cysteine containing proteins of the gelatin were successfully addressed by complexation with meso-2,3-dimercaptosuccinic acid (DMSA). No mercury was detected in the cere...
Frederick P. Roth - One of the best experts on this subject based on the ideXlab platform.
-
A genome-wide gene function prediction resource for Drosophila Melanogaster.
PLoS ONE, 2010Co-Authors: Han Yan, John E. Beaver, Kavitha Venkatesan, Niels Klitgord, Muhammed A. Yildirim, Tong Hao, David E. Hill, Michael E. Cusick, Norbert Perrimon, Frederick P. RothAbstract:Predicting gene functions by integrating large-scale biological data remains a challenge for systems biology. Here we present a resource for Drosophila Melanogaster gene function predictions. We trained function-specific classifiers to optimize the influence of different biological datasets for each functional category. Our model predicted GO terms and KEGG pathway memberships for Drosophila Melanogaster genes with high accuracy, as affirmed by cross-validation, supporting literature evidence, and large-scale RNAi screens. The resulting resource of prioritized associations between Drosophila genes and their potential functions offers a guide for experimental investigations.
