The Experts below are selected from a list of 174 Experts worldwide ranked by ideXlab platform
Hao Chen - One of the best experts on this subject based on the ideXlab platform.
-
cytotoxicity and biocompatibility evaluation of n o carboxymethyl chitosan oxidized alginate hydrogel for Drug Delivery Application
International Journal of Biological Macromolecules, 2012Co-Authors: Xiangye Kong, Zhaoliang Zhang, Kaihui Nan, Xianhou Wang, Hao ChenAbstract:In this paper, covalently cross-linked hydrogel composed of N,O-carboxymethyl chitosan and oxidized alginate was developed intending for Drug Delivery Application. In vitro/vivo cytocompatibility and biocompatibility of the developed hydrogel were preliminary evaluated. In vitro cytocompatibility test showed that the developed hydrogel exhibited good cytocompatibility against NH3T3 cells after 3-day incubation. According to the results of acute toxicity test, there was no obvious cytotoxicity for major organs during the period of 21-day intraperitoneal administration. Meanwhile, the developed hydrogel did not induce any cutaneous reaction within 72 h of subcutaneous injection followed by slow degradation and adsorption with the time evolution. Moreover, the extraction of developed hydrogel had nearly 0% of hemolysis ratio, which indicated the good hemocompatibility of hydrogel. Based on the above results, it may be concluded that the developed N,O-carboxymethyl chitosan/oxidized alginate hydrogel with non-cytotoxicity and good biocompatibility might suitable for the various Drug Delivery Applications.
-
Cytotoxicity and biocompatibility evaluation of N,O-carboxymethyl chitosan/oxidized alginate hydrogel for Drug Delivery Application.
International journal of biological macromolecules, 2012Co-Authors: Xiangye Kong, Zhaoliang Zhang, Kaihui Nan, Xianhou Wang, Hao ChenAbstract:In this paper, covalently cross-linked hydrogel composed of N,O-carboxymethyl chitosan and oxidized alginate was developed intending for Drug Delivery Application. In vitro/vivo cytocompatibility and biocompatibility of the developed hydrogel were preliminary evaluated. In vitro cytocompatibility test showed that the developed hydrogel exhibited good cytocompatibility against NH3T3 cells after 3-day incubation. According to the results of acute toxicity test, there was no obvious cytotoxicity for major organs during the period of 21-day intraperitoneal administration. Meanwhile, the developed hydrogel did not induce any cutaneous reaction within 72 h of subcutaneous injection followed by slow degradation and adsorption with the time evolution. Moreover, the extraction of developed hydrogel had nearly 0% of hemolysis ratio, which indicated the good hemocompatibility of hydrogel. Based on the above results, it may be concluded that the developed N,O-carboxymethyl chitosan/oxidized alginate hydrogel with non-cytotoxicity and good biocompatibility might suitable for the various Drug Delivery Applications.
Rakesh Gupta - One of the best experts on this subject based on the ideXlab platform.
-
Computer-Aided Design of Nanoparticles for Transdermal Drug Delivery
Drug Delivery Systems, 2020Co-Authors: Rakesh Gupta, Beena RaiAbstract:Human skin provides an excellent opportunity for Drug Delivery Application. However, the Delivery of hydrophilic Drug and big protein molecules is challenging due to barrier provided by the top layer of skin known as stratum corneum (SC). The chemical permeation enhancers or specialized carriers such as nanoparticles (NPs) are needed which can deliver Drug molecules into the deeper layer. Here, we describe the in silico design of nanoparticle carriers using molecular dynamics (MD) simulations for the transdermal Drug Delivery Application. At first, setup of a skin lipid bilayer model is demonstrated. Further, nanoparticles are designed based on the Monte Carlo simulation technique. These nanoparticles are then tested on skin model using various MD simulation techniques.
-
In-silico design of nanoparticles for transdermal Drug Delivery Application.
Nanoscale, 2018Co-Authors: Rakesh GuptaAbstract:Nanoparticles are used in the medical field for various Applications like cell imaging, Drug Delivery, gene and si-RNA Delivery, to name a few. Designing nanoparticles for a given Application, purely based on the trial and error experimentation, requires a lot of time and effort. In this study we show that computer simulations could help in designing nanoparticles for Drug Delivery thus reducing the time and cost associated with their design, development and deployment. The permeation of nanoparticles, having various surface chemistries and patterns, through the skin lipid bilayer was studied using constrained and unconstrained molecular dynamics simulations. Interestingly, the permeation mechanism of nanoparticles having the same surface chemistry but different patterns was found to be completely different. Nanoparticles (NPs) were screened based on the free energy of permeation through the skin lipid bilayer. The behavior of the screened NPs was further validated with unconstrained simulations using the skin lipid bilayer. Nanoparticles thus screened through both of the techniques were further used for the co-Delivery of a model protein into the skin lipid bilayer. It was observed that the nanoparticles having a 2 : 1 homogeneous ratio of hydrophobic to hydrophilic regions were the most promising in transdermal Delivery of proteins. The obtained results are in line with the results of recent permeation experiments on cell and plasma membrane. Our study could help in in-silico design of nanoparticles for Delivery of actives through skin. These in-silico experiments thus could help speed up the development process by guiding formulation chemists.
-
effect of size and surface charge of gold nanoparticles on their skin permeability a molecular dynamics study
Scientific Reports, 2017Co-Authors: Rakesh GuptaAbstract:Molecular level understanding of permeation of nanoparticles through human skin establishes the basis for development of novel transdermal Drug Delivery systems and design and formulation of cosmetics. Recent experiments suggest that surface coated nano-sized gold nanoparticles (AuNPs) can penetrate the rat and human skin. However, the mechanisms by which these AuNPs penetrate are not well understood. In this study, we have carried out coarse grained molecular dynamics simulations to explore the permeation of dodecanethiol coated neutral hydrophobic AuNPs of different sizes (2–5 nm) and surface charges (cationic and anionic) through the model skin lipid membrane. The results indicate that the neutral hydrophobic AuNPs disrupted the bilayer and entered in it with in ~200 ns, while charged AuNPs were adsorbed on the bilayer headgroup. The permeation free energy calculation revealed that at the head group of the bilayer, a very small barrier existed for neutral hydrophobic AuNP while a free energy minimum was observed for charged AuNPs. The permeability was maximum for neutral 2 nm gold nanoparticle (AuNP) and minimum for 3 nm cationic AuNP. The obtained results are aligned with recent experimental findings. This study would be helpful in designing customized nanoparticles for cosmetic and transdermal Drug Delivery Application.
Xiangye Kong - One of the best experts on this subject based on the ideXlab platform.
-
cytotoxicity and biocompatibility evaluation of n o carboxymethyl chitosan oxidized alginate hydrogel for Drug Delivery Application
International Journal of Biological Macromolecules, 2012Co-Authors: Xiangye Kong, Zhaoliang Zhang, Kaihui Nan, Xianhou Wang, Hao ChenAbstract:In this paper, covalently cross-linked hydrogel composed of N,O-carboxymethyl chitosan and oxidized alginate was developed intending for Drug Delivery Application. In vitro/vivo cytocompatibility and biocompatibility of the developed hydrogel were preliminary evaluated. In vitro cytocompatibility test showed that the developed hydrogel exhibited good cytocompatibility against NH3T3 cells after 3-day incubation. According to the results of acute toxicity test, there was no obvious cytotoxicity for major organs during the period of 21-day intraperitoneal administration. Meanwhile, the developed hydrogel did not induce any cutaneous reaction within 72 h of subcutaneous injection followed by slow degradation and adsorption with the time evolution. Moreover, the extraction of developed hydrogel had nearly 0% of hemolysis ratio, which indicated the good hemocompatibility of hydrogel. Based on the above results, it may be concluded that the developed N,O-carboxymethyl chitosan/oxidized alginate hydrogel with non-cytotoxicity and good biocompatibility might suitable for the various Drug Delivery Applications.
-
Cytotoxicity and biocompatibility evaluation of N,O-carboxymethyl chitosan/oxidized alginate hydrogel for Drug Delivery Application.
International journal of biological macromolecules, 2012Co-Authors: Xiangye Kong, Zhaoliang Zhang, Kaihui Nan, Xianhou Wang, Hao ChenAbstract:In this paper, covalently cross-linked hydrogel composed of N,O-carboxymethyl chitosan and oxidized alginate was developed intending for Drug Delivery Application. In vitro/vivo cytocompatibility and biocompatibility of the developed hydrogel were preliminary evaluated. In vitro cytocompatibility test showed that the developed hydrogel exhibited good cytocompatibility against NH3T3 cells after 3-day incubation. According to the results of acute toxicity test, there was no obvious cytotoxicity for major organs during the period of 21-day intraperitoneal administration. Meanwhile, the developed hydrogel did not induce any cutaneous reaction within 72 h of subcutaneous injection followed by slow degradation and adsorption with the time evolution. Moreover, the extraction of developed hydrogel had nearly 0% of hemolysis ratio, which indicated the good hemocompatibility of hydrogel. Based on the above results, it may be concluded that the developed N,O-carboxymethyl chitosan/oxidized alginate hydrogel with non-cytotoxicity and good biocompatibility might suitable for the various Drug Delivery Applications.
Nadia Rasool - One of the best experts on this subject based on the ideXlab platform.
-
synthesis and characterization of novel ph ionic strength and temperature sensitive hydrogel for insulin Delivery
Polymer, 2010Co-Authors: Nadia Rasool, Tariq Yasin, Jerry Y Y Heng, Zareen AkhterAbstract:Abstract A series of novel silane crosslinked hydrogel was prepared from kappa carrageenan (KC), acrylic acid (AA) using vinyltriethoxysilane (VTESi). Potassium persulphate initiated the grafting and copolymerization reactions between reactants. In addition, the condensation of the hydroxyl groups of KC and VTESi resulted into crosslinking. Novelty of this work is the use of VTESi as crosslinker for such a composition of hydrogel. The structure of prepared hydrogels was characterized by Fourier transform infrared spectroscopy. The analysis of spectra confirmed the presence of feed components in the prepared hydrogels. Thermogravimetric analysis showed an increase in the stability of the hydrogels either having high AA contents or crosslinker amount. The effect of feed components, pH (buffer, non-buffer), electrolytic media and temperature on the swelling behaviour of the hydrogels is reported here. Most promising results with high swelling ratio were observed in hydrogel having low monomeric ratio (KC:AA = 1:7). pH response of this hydrogel in acidic and neutral pH makes it suitable for Drug Delivery Application. Insulin, a protein based Drug was selected as a model Drug. It requires its Delivery in small intestine for proper action; therefore its release behaviour was studied in-vitro in simulated stomach and intestinal fluids. The release profile of insulin showed negligible release in simulated gastric fluid (SGF) and sustained release in simulated intestinal fluid (SIF). The obtained results are in good agreement with the swelling response of this hydrogel. The weak structure of this hydrogel makes it preferable for Drug Delivery, as it is able to get crumbled after releasing the Drug for 6 h at neutral pH.
Jun Lin - One of the best experts on this subject based on the ideXlab platform.
-
stimuli responsive Drug Delivery Application of polymer and silica in biomedicine
Journal of Materials Chemistry B, 2015Co-Authors: Arif Gulzar, Shili Gai, Piaoping Yang, Mohd Bismillah Ansari, Jun LinAbstract:In the last decade, using polymer and mesoporous silica materials as efficient Drug Delivery carriers has attracted great attention. Although the development and Application of them involves some inevitable barriers, such as chronic toxicities, long-term stability, understanding of the biological fate and physiochemical properties, biodistribution, effect in the biological environment, circulation properties and targeting efficacy in vivo. The construction of stimuli responsive Drug carriers using biologically safe materials, followed by hydrophilic modification, bioconjugation, targeting functionalization, and detailed safety analysis in small/large animal models may be the best way to overcome these barriers. Huge progress has been made in stimuli responsive Drug Delivery systems based on polymer and mesoporous silica materials, mainly including pH-, thermo-, light-, enzyme-, redox-, magnetic field- and ultrasound-responsive Drug Delivery systems, all of which are highlighted in this review.
