The Experts below are selected from a list of 126 Experts worldwide ranked by ideXlab platform
Catherine E. Ovitt - One of the best experts on this subject based on the ideXlab platform.
-
Ascl3 expression marks a progenitor population of both acinar and Ductal Cells in mouse salivary glands
Developmental biology, 2008Co-Authors: Tara A. Bullard, Laurie L. Koek, Elisa Roztocil, Paul D. Kingsley, Lily Mirels, Catherine E. OvittAbstract:Ascl3, also know as Sgn1, is a member of the mammalian achaete scute (Mash) gene family of transcription factors, which have been implicated in Cell fate specification and differentiation. In the mouse salivary gland, expression of Ascl3 is restricted to a subset of duct Cells. Salivary gland function depends on the secretory acinar Cells, which are responsible for saliva formation, and duct Cells, which modify the saliva and conduct it to the oral cavity. The salivary gland ducts are also the putative site of progenitor Cells in the adult gland. Using a Cre recombinase-mediated reporter system, we followed the fate of Ascl3-expressing Cells after the introduction of an EGFP-Cre expression cassette into the Ascl3 locus by homologous recombination. Lineage tracing shows that these Cells are progenitors of both acinar and Ductal Cell types in all three major salivary glands. In the differentiated progeny, expression of Ascl3 is down-regulated. These data directly demonstrate a progenitor-progeny relationship between duct Cells and the acinar Cell compartment, and identify a population of multipotent progenitor Cells, marked by expression of Ascl3, which is capable of generating both gland Cell types. We conclude that Ascl3-expressing Cells contribute to the maintenance of the adult salivary glands.
Patricia A Denny - One of the best experts on this subject based on the ideXlab platform.
-
Evidence of a phenotypically determined Ductal Cell lineage in mouse salivary glands.
The Anatomical record, 1999Co-Authors: Paul C Denny, Peixin Liu, Patricia A DennyAbstract:The submandibular salivary gland of mice contains a parenchymal element, the granular duct, which matures peripubertally from the striated ducts. Granular duct Cells also differentiate from intercalated ducts in the adult mouse submandibular gland. Using preproNGF-A as a signature protein of mature granular duct Cells, this study inquired if phenotypic determination might have occurred earlier than the first signs of Cellular differentiation. Results from RT-PCR indicate the presence of preproNGF-A transcripts at all postnatal stages of development of the submandibular glands, as well as in adult sublingual glands. The preproNGF-A transcript was also detected prenatally as early as embryonic day 17 in the submandibular/sublingual complex. Using an antibody directed specifically against the “pre” peptide, immunocytochemistry showed preproNGF-A localized in the granular ducts and striated ducts of the adult submandibular gland. In addition preproNGF-A was detected throughout the first order branches of the intercalated duct system. In the neonatal gland, preproNGF-A was found in the large tubules that differentiate to the striated ducts. The early appearance of preproNGF-A in the histological lineage that sequentially gives rise to striated ducts and then to granular ducts suggests that this lineage is phenotypically determined as early as birth. An undifferentiated stage of the phenotypically determined lineage also appears to be retained in the intercalated duct system to provide progenitors for subsequent differentiation in the adult gland. Throughout development of the sublingual gland, preproNGF-A was detectable in the striated ducts or in their predecessors, suggesting that they may also represent a phenotypically determined Cell lineage similar to that of the submandibular gland. Anat Rec 256:84–90, 1999. © 1999 Wiley-Liss, Inc.
Tara A. Bullard - One of the best experts on this subject based on the ideXlab platform.
-
Ascl3 expression marks a progenitor population of both acinar and Ductal Cells in mouse salivary glands
Developmental biology, 2008Co-Authors: Tara A. Bullard, Laurie L. Koek, Elisa Roztocil, Paul D. Kingsley, Lily Mirels, Catherine E. OvittAbstract:Ascl3, also know as Sgn1, is a member of the mammalian achaete scute (Mash) gene family of transcription factors, which have been implicated in Cell fate specification and differentiation. In the mouse salivary gland, expression of Ascl3 is restricted to a subset of duct Cells. Salivary gland function depends on the secretory acinar Cells, which are responsible for saliva formation, and duct Cells, which modify the saliva and conduct it to the oral cavity. The salivary gland ducts are also the putative site of progenitor Cells in the adult gland. Using a Cre recombinase-mediated reporter system, we followed the fate of Ascl3-expressing Cells after the introduction of an EGFP-Cre expression cassette into the Ascl3 locus by homologous recombination. Lineage tracing shows that these Cells are progenitors of both acinar and Ductal Cell types in all three major salivary glands. In the differentiated progeny, expression of Ascl3 is down-regulated. These data directly demonstrate a progenitor-progeny relationship between duct Cells and the acinar Cell compartment, and identify a population of multipotent progenitor Cells, marked by expression of Ascl3, which is capable of generating both gland Cell types. We conclude that Ascl3-expressing Cells contribute to the maintenance of the adult salivary glands.
Anil K. Rustgi - One of the best experts on this subject based on the ideXlab platform.
-
Culturing primary mouse pancreatic Ductal Cells.
Cold Spring Harbor protocols, 2015Co-Authors: Maximilian Reichert, Andrew D. Rhim, Anil K. RustgiAbstract:The most common subtype of pancreatic cancer is pancreatic Ductal adenocarcinoma (PDAC). PDAC resembles Ductal Cells morphologically. To study pancreatic Ductal Cell (PDC) and pancreatic intraepithelial neoplasia (PanIN)/PDAC biology, it is essential to have reliable in vitro culture conditions. Here we describe a methodology to isolate, culture, and passage PDCs and duct-like Cells from the mouse pancreas. It can be used to isolate Cells from genetically engineered mouse models (GEMMs), providing a valuable tool to study disease models in vitro to complement in vivo findings. The culture conditions allow epithelial Cells to outgrow fibroblast and other "contaminating" Cell types within a few passages. However, the resulting cultures, although mostly epithelial, are not completely devoid of fibroblasts. Regardless, this protocol provides guidelines for a robust in vitro culture system to isolate, maintain, and expand primary pancreatic Ductal epithelial Cells. It can be applied to virtually all GEMMs of pancreatic disease and other diseases and cancers that arise from Ductal structures. Because most carcinomas resemble Ductal structures, this protocol has utility in the study of other cancers in addition to PDAC, such as breast and prostate cancers.
Thomas Magnuson - One of the best experts on this subject based on the ideXlab platform.
-
Effect of reg protein on rat pancreatic Ductal Cells.
Pancreas, 1998Co-Authors: Michael E. Zenilman, Jian Chen, Thomas MagnusonAbstract:The pancreatic regenerating gene (reg I) is expressed in the exocrine pancreas and is involved in islet regeneration. Reg I protein has been shown to be mitogenic to beta- and Ductal Cell lines, but not mature islets. In this study, we tested the effect of two isolates of reg I on primary cultures of Ductal Cells. Rat pancreatic Ductal Cells were isolated by collagenase digestion and isolated colonies were maintained in culture. The Cells were proven to be Ductal in origin by their morphology and by immunofluorescent staining with epithelial markers. Reg I was isolated from human pancreatic extracts or from the rat acinar Cell line AR42J by sequential ammonium sulfate precipitation and acid precipitation. Cells were cultured with doses of reg I for 72 h, pulsed with 10 microM bromodeoxyuridine (BrdU) for 2 h. After fixation, nuclei were double-stained with propidium iodide and BrdU monoclonal antibody. The percentages of nuclei positive for BrdU were calculated from at least five colonies per group. A 10-nM concentration of human reg I increased BrdU incorporation by 2.3-fold over controls, rat reg I increased it by 1.4-fold (p < 0.05). When compared to their effects on the Ductal Cell line ARIP, both human and rat reg I were 100 times more potent on the primary cultures of Ductal Cells. We conclude that human and rat reg I proteins are mitogenic to primary cultures of Ductal Cells. Although principally a product of the acinar Cell, reg I appears to be a stimulus of Ductal Cell growth and, in this fashion, may modulate the expansion of the pancreatic Ductal population during islet regeneration.
