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Amir Fazeli - One of the best experts on this subject based on the ideXlab platform.
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Carotenoids accumulation by Dunaliella tertiolecta (Lake Urmia isolate) and Dunaliella Salina (CCAP 19/18 & WT) under stress conditions
DARU Journal of Pharmaceutical Sciences, 2006Co-Authors: Mohammad Reza Fazeli, Hossein Tofighi, Nasrin Samadi, Hossein Jamalifar, Amir FazeliAbstract:Carotenoids are widely used in nutraceutical, additives and cosmetics as colorants and antioxidants. Dunaliella is the main natural source which accumulates massive amount of carotenoids. This study examines the effect of different concentrations of NaCl (0.3M to 2M) and light intensities of 50 and 150 μmol/m2s on total carotenoids accumulated by Dunaliella tertiolecta (DCCBC26), from Urmia lake (North West of Iran) compared to those obtained from Dunaliella Salina CCAP 19/18 and also the wild type Dunaliella Salina WT strains. In all microalgaes production of carotenoids were triggered by increase in light intensities. Changes of intensity from 50 μmol/m2s to 150 μmol/m2s led to 2.4, 2.1 and 1.4 folds of carotenoids production by D. Salina CCAP, D. Salina WT and D. tertiolecta respectively. In both Salina strains carotenoids production improved with higher salinity picking at salt concentration of 2M, while D. tertiolecta showed optimum carotenoids production at 0.7M.
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Carotenoids accumulation by Dunaliella tertiolecta (lake Urmia isolate) and Dunaliella Salina (ccap 19/18 & WT) under stree conditions
DARU, 2006Co-Authors: Mohammad Reza Fazeli, Hossein Tofighi, Nasrin Samadi, Hossein Jamalifar, Amir FazeliAbstract:Carotenoids are widely used in nutraceutical, additives and cosmetics as colorants and antioxidants. Dunaliella is the main natural source which accumulates massive amount of carotenoids. This study examines the effect of different concentrations of NaCl (0.3M to 2M) and light intensities of 50 and 150 μmol/m2s on total carotenoids accumulated by Dunaliella tertiolecta (DCCBC26), from Urmia lake (North West of Iran) compared to those obtained from Dunaliella Salina CCAP 19/18 and also the wild type Dunaliella Salina WT strains. In all microalgaes production of carotenoids were triggered by increase in light intensities. Changes of intensity from 50 μmol/m2s to 150 μmol/m2s led to 2.4, 2.1 and 1.4 folds of carotenoids production by D. Salina CCAP, D. Salina WT and D. tertiolecta respectively. In both Salina strains carotenoids production improved with higher salinity picking at salt concentration of 2M, while D. tertiolecta showed optimum carotenoids production at 0.7M.
Rene H Wijffels - One of the best experts on this subject based on the ideXlab platform.
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carotenoid fluorescence in Dunaliella Salina
Journal of Applied Phycology, 2010Co-Authors: Dorinde M M Kleinegris, Marcel Janssen, Willem A Brandenburg, Rene H WijffelsAbstract:Dunaliella Salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid globules in vivo. In this study, we show that the carotenoid-rich globules emit clear green fluorescence, which can be used in, for example, fluorescence microscopy (e.g. CLSM) to obtain pictures of the cells and their carotenoid content.
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the selectivity of milking of Dunaliella Salina
Marine Biotechnology, 2010Co-Authors: Dorinde M M Kleinegris, Marcel Janssen, Willem A Brandenburg, Rene H WijffelsAbstract:The process of the simultaneous production and extraction of carotenoids, milking, of Dunaliella Salina was studied. We would like to know the selectivity of this process. Could all the carotenoids produced be extracted? And would it be possible to vary the profile of the produced carotenoids and, consequently, influence the type of carotenoids extracted? By using three different D. Salina strains and three different stress conditions, we varied the profiles of the carotenoids produced. Between Dunaliella bardawil and D. Salina 19/18, no remarkable differences were seen in the extraction profiles, although D. Salina 19/18 seemed to be better extractable. D. Salina 19/25 was not “milkable” at all. The milking process could only be called selective for secondary carotenoids in case gentle mixing was used. In aerated flat-panel photobioreactors, extraction was much better, but selectiveness decreased and also chlorophyll and primary carotenoids were extracted. This was possibly related to cell damage due to shear stress.
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selective extraction of carotenoids from the microalga Dunaliella Salina with retention of viability
Biotechnology and Bioengineering, 2002Co-Authors: Mohammad Amin Hejazi, C De Lamarliere, Jorge Rocha, M H Vermue, J Tramper, Rene H WijffelsAbstract:Simultaneous production and selective extraction of -carotene from living cells of Dunaliella Salina in a two-phase system of aqueous and organic phases has been investigated. Solvents with values of log Poctanol, which denotes hydrophobicity of a compound, ranging from 3 to 9 were used as organic phase. Viability and activity of Dunaliella Salina in the presence of organic solvents were checked by microscopic observation and photosynthetic oxygen-production-rate measurements, respectively. Extraction ability of different solvents for both -carotene and chlorophyll was determined spectrophotometrically. In addition, -carotene contents of the cells growing in the aqueous phase and extracted -carotene by the different organic phases were quantified by the same method. Results showed that solvents having log Poctanol > 6 can be considered biocompatible for this alga. Moreover, pigment extraction ability of a solvent is inversely dependent on its log Poctanol value. By increasing the degenerative hydrophobicity the extraction ability for both chlorophyll and -carotene, decreases. However, this decrease is more profound for chlorophyll. Therefore, selective extraction of -carotene becomes feasible. Comparison of the total -carotene produced in the presence and in the absence of solvents shows that the presence of a second phase of biocompatible solvents in the culture media may induce the -carotene production pathway. The -carotene productivity per cell in a two-phase system with dodecane was the highest observed. Extraction ability of the biocompatible solvents dodecane, tetradecan, and hexadecane was similar
Yang Xiao-ling - One of the best experts on this subject based on the ideXlab platform.
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Movement Characteristic of Dunaliella Salina Cell and Its Affecting Factors
Journal of Hydroecology, 2011Co-Authors: Yang Xiao-lingAbstract:In order to reveal movement characteristics of Dunaliella Salina cell under different conditions,the density change of Dunaliella Salina cells at ends U bend was studied.The results showed that the movement of Dunaliella Salina cells was influenced by light,ATP,sodium malonate and viscosity.Dunaliella Salina cells had the movement characteristic to light gather.Cell movement can be speeded up by adding ATP made,and can be slowed by adding sodium malonate,Dissipation energy took place in Dunaliella Salina cells movement;when viscosity of medium was increased,cell movement would be slowed.
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Study on Regulatory Effect of 2,4-D on Growth of Dunaliella Salina
Journal of Anhui Agricultural Sciences, 2010Co-Authors: Yang Xiao-lingAbstract:[Objective]The aim was to study regulatory effect of 2,4-D on growth of Dunaliella Salina.[Method]Effect of different concentrations 2,4-D on growth of Dunaliella Salina was studied.[Result]The growth and material accumulation of Dunaliella Salina could be promoted in 2,4-D concentration of 1.0-6.0 mg/L.When the 2,4-D was in a concentration of 4.0 mg/L in culture medium,the effects were most advantageous to grow,which made cellular density,protein and β-carotene accumulation of Dunaliella Salina reach high limit.[Conclusion]2,4-D could be used for regulate growth of Dunaliella Salina during culture.
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Research of treatment conditions for purification superoxide dismutase in Dunaliella Salina
Marine Science Bulletin, 2010Co-Authors: Yang Xiao-lingAbstract:In order to research and utilize SOD in Dunaliella Salina,it is necessary for us to establish the method of purification the SOD in Dunaliella Salina. Experiment studied treatment conditions for Purification the SOD in Dunaliella Salina. Results show that under the following conditions: the SOD crude extracts heated for 20 min at 70℃ ; the supernatant liquid added acetone of 0.4 times and -35℃ was precipitated; the precipitated SOD dissolved in the 20 mM, pH 7.6 PBS buffer and separated by DEAE-52 column chromatography; adopting a method of gradient elution SOD washed with combination ddH2O+1.00mol/L NaCI-0.02 mol/L PBS (pH 7.6)from column, the SOD in Dunaliella Salina can be efficiently separated and purified from its protein. The recovery rate was about 60%, and purification multiplier was as high as 413.
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Effects on Growth and Accumulation of Protein and β-carotene in Dunaliella Salina Influenced by Combination of Zn and Mn
Journal of Hydroecology, 2010Co-Authors: Yang Xiao-lingAbstract:In order to research the microelement concentration to Dunaliella Salina cell cultured,the experiment study on that effects on growth and material accumulation of Dunaliella Salina influenced by different concentration combination of Zn and Mn.The results showed that adequate concentration of Zn and MnCl2·4H2O had improving to Dunaliella Salina cell growth and material accumulation,it was disadvantageous when concentration was too high or too low.when the combination was in a ZnSO4·7H2O concentration of 6 mg/L and Mn concentration of 4 mg/L in culture medium,the effects were most advantageous to growth,accumulation of protein and β-carotene in Dunaliella Salina in 9 different patterns.Made cellular density,protein and β-carotene accumulation of Dunaliella Salina to reach high limit among.When the combination was in a ZnSO4·7H2O concentration of 8 mg/L and MnCl2 ·4H2O concentration of6 mg/L,they made protein and β-carotene accumulation in single cell of Dunaliella Salina to reach high limit among.The latter may be a response adaptation of Dunaliella Salina cell to adversity of too much Zn and Mn.
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Extraction and separation of Dunaliella Salina protein
Food Science and Technology International, 2010Co-Authors: Yang Xiao-lingAbstract:Using ultrasonication method Dunaliella protein was extracted,yield is 40% in Dunaliella cells.Extracting Dunaliella protein was separated through Sephacryl S-300 gel column chromatography,and form 3-eluting peaks.The result indicated that Dunaliella cells proteins can be divided into 3 kinds big,the total yield of protein chromatography is 92.0%.Eluting peaks-I continues polyacrylamide gel electrophoresis(SDS-PAGE) and there are at least nine kinds protein of different quality by SDS-PAGE.This may show Dunaliella Salina protein diversity.
Mohammad Reza Fazeli - One of the best experts on this subject based on the ideXlab platform.
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Carotenoids accumulation by Dunaliella tertiolecta (Lake Urmia isolate) and Dunaliella Salina (CCAP 19/18 & WT) under stress conditions
DARU Journal of Pharmaceutical Sciences, 2006Co-Authors: Mohammad Reza Fazeli, Hossein Tofighi, Nasrin Samadi, Hossein Jamalifar, Amir FazeliAbstract:Carotenoids are widely used in nutraceutical, additives and cosmetics as colorants and antioxidants. Dunaliella is the main natural source which accumulates massive amount of carotenoids. This study examines the effect of different concentrations of NaCl (0.3M to 2M) and light intensities of 50 and 150 μmol/m2s on total carotenoids accumulated by Dunaliella tertiolecta (DCCBC26), from Urmia lake (North West of Iran) compared to those obtained from Dunaliella Salina CCAP 19/18 and also the wild type Dunaliella Salina WT strains. In all microalgaes production of carotenoids were triggered by increase in light intensities. Changes of intensity from 50 μmol/m2s to 150 μmol/m2s led to 2.4, 2.1 and 1.4 folds of carotenoids production by D. Salina CCAP, D. Salina WT and D. tertiolecta respectively. In both Salina strains carotenoids production improved with higher salinity picking at salt concentration of 2M, while D. tertiolecta showed optimum carotenoids production at 0.7M.
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Carotenoids accumulation by Dunaliella tertiolecta (lake Urmia isolate) and Dunaliella Salina (ccap 19/18 & WT) under stree conditions
DARU, 2006Co-Authors: Mohammad Reza Fazeli, Hossein Tofighi, Nasrin Samadi, Hossein Jamalifar, Amir FazeliAbstract:Carotenoids are widely used in nutraceutical, additives and cosmetics as colorants and antioxidants. Dunaliella is the main natural source which accumulates massive amount of carotenoids. This study examines the effect of different concentrations of NaCl (0.3M to 2M) and light intensities of 50 and 150 μmol/m2s on total carotenoids accumulated by Dunaliella tertiolecta (DCCBC26), from Urmia lake (North West of Iran) compared to those obtained from Dunaliella Salina CCAP 19/18 and also the wild type Dunaliella Salina WT strains. In all microalgaes production of carotenoids were triggered by increase in light intensities. Changes of intensity from 50 μmol/m2s to 150 μmol/m2s led to 2.4, 2.1 and 1.4 folds of carotenoids production by D. Salina CCAP, D. Salina WT and D. tertiolecta respectively. In both Salina strains carotenoids production improved with higher salinity picking at salt concentration of 2M, while D. tertiolecta showed optimum carotenoids production at 0.7M.
Hossein Jamalifar - One of the best experts on this subject based on the ideXlab platform.
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Carotenoids accumulation by Dunaliella tertiolecta (Lake Urmia isolate) and Dunaliella Salina (CCAP 19/18 & WT) under stress conditions
DARU Journal of Pharmaceutical Sciences, 2006Co-Authors: Mohammad Reza Fazeli, Hossein Tofighi, Nasrin Samadi, Hossein Jamalifar, Amir FazeliAbstract:Carotenoids are widely used in nutraceutical, additives and cosmetics as colorants and antioxidants. Dunaliella is the main natural source which accumulates massive amount of carotenoids. This study examines the effect of different concentrations of NaCl (0.3M to 2M) and light intensities of 50 and 150 μmol/m2s on total carotenoids accumulated by Dunaliella tertiolecta (DCCBC26), from Urmia lake (North West of Iran) compared to those obtained from Dunaliella Salina CCAP 19/18 and also the wild type Dunaliella Salina WT strains. In all microalgaes production of carotenoids were triggered by increase in light intensities. Changes of intensity from 50 μmol/m2s to 150 μmol/m2s led to 2.4, 2.1 and 1.4 folds of carotenoids production by D. Salina CCAP, D. Salina WT and D. tertiolecta respectively. In both Salina strains carotenoids production improved with higher salinity picking at salt concentration of 2M, while D. tertiolecta showed optimum carotenoids production at 0.7M.
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Carotenoids accumulation by Dunaliella tertiolecta (lake Urmia isolate) and Dunaliella Salina (ccap 19/18 & WT) under stree conditions
DARU, 2006Co-Authors: Mohammad Reza Fazeli, Hossein Tofighi, Nasrin Samadi, Hossein Jamalifar, Amir FazeliAbstract:Carotenoids are widely used in nutraceutical, additives and cosmetics as colorants and antioxidants. Dunaliella is the main natural source which accumulates massive amount of carotenoids. This study examines the effect of different concentrations of NaCl (0.3M to 2M) and light intensities of 50 and 150 μmol/m2s on total carotenoids accumulated by Dunaliella tertiolecta (DCCBC26), from Urmia lake (North West of Iran) compared to those obtained from Dunaliella Salina CCAP 19/18 and also the wild type Dunaliella Salina WT strains. In all microalgaes production of carotenoids were triggered by increase in light intensities. Changes of intensity from 50 μmol/m2s to 150 μmol/m2s led to 2.4, 2.1 and 1.4 folds of carotenoids production by D. Salina CCAP, D. Salina WT and D. tertiolecta respectively. In both Salina strains carotenoids production improved with higher salinity picking at salt concentration of 2M, while D. tertiolecta showed optimum carotenoids production at 0.7M.
