The Experts below are selected from a list of 3858 Experts worldwide ranked by ideXlab platform
Cristina Dehelean - One of the best experts on this subject based on the ideXlab platform.
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Changes in the anti-inflammatory activity of soy isoflavonoid genistein versus genistein incorporated in two types of cyclodextrin derivatives
Chemistry Central Journal, 2012Co-Authors: Corinatiulea Danciu, Codruta Soica, Erzsébet Csányi, Rita Ambrus, Stefana Feflea, Camelia Peev, Cristina DeheleanAbstract:The isoflavonoid genistein represents the major active compound from soybean, the vegetal product from Glycine max (Fabaceae). The aim of this study is to prove that genistein was incorporated in two semisynthetic cyclodextrins, beta-cyclodextrin derivatives: hydroxypropyl-beta-cyclodextrin and randomly-methylated-beta-cyclodextrin as well as to compare the anti-inflammatory activity of genistein with that of genistein incorporated in these two types of semisynthetic cyclodextrins. The animal studies were conducted on 8-week old C57BL/6 J female mice. Inflammation was induced in both Ears of each mouse by topical application of 10 micrograms 12-O-tetradecanoylphorbol-3-acetate dissolved in 0.1 ml solvent (acetone : dimethylsulfoxide in a molar ratio 9:1). Thirty minutes later treatment was applied. The inflammatory reaction was correlated with increased values in Ear Thickness. Treatment with genistein and genistein incorporated in the two cyclodextrins led to decreased values for Ear Thickness. Better anti-inflammatory action was found for the complexes of genistein. Both haematoxylin-eosin analysis and CD45 marker expression are in agreement with these findings. Results allow concluding that genistein is an active anti-inflammatory phytocompound and its complexation with hydrophilic beta-cyclodextrin derivatives leads to a stronger anti-inflammatory activity.
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Changes in the anti-inflammatory activity of soy isoflavonoid genistein versus genistein incorporated in two types of cyclodextrin derivatives
Chemistry Central Journal, 2012Co-Authors: Corinatiulea Danciu, Codruta Soica, Erzsébet Csányi, Rita Ambrus, Stefana Feflea, Camelia Peev, Cristina DeheleanAbstract:Background The isoflavonoid genistein represents the major active compound from soybean, the vegetal product from Glycine max (Fabaceae) . The aim of this study is to prove that genistein was incorporated in two semisynthetic cyclodextrins, beta-cyclodextrin derivatives: hydroxypropyl-beta-cyclodextrin and randomly-methylated-beta-cyclodextrin as well as to compare the anti-inflammatory activity of genistein with that of genistein incorporated in these two types of semisynthetic cyclodextrins. Results The animal studies were conducted on 8-week old C57BL/6 J female mice. Inflammation was induced in both Ears of each mouse by topical application of 10 micrograms 12-O-tetradecanoylphorbol-3-acetate dissolved in 0.1 ml solvent (acetone : dimethylsulfoxide in a molar ratio 9:1). Thirty minutes later treatment was applied. The inflammatory reaction was correlated with increased values in Ear Thickness. Treatment with genistein and genistein incorporated in the two cyclodextrins led to decreased values for Ear Thickness. Better anti-inflammatory action was found for the complexes of genistein. Both haematoxylin-eosin analysis and CD45 marker expression are in agreement with these findings. Conclusions Results allow concluding that genistein is an active anti-inflammatory phytocompound and its complexation with hydrophilic beta-cyclodextrin derivatives leads to a stronger anti-inflammatory activity.
Haruhiko Manabe - One of the best experts on this subject based on the ideXlab platform.
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Effect of orally administered rolipram, a phosphodiesterase 4 inhibitor, on a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application
European Journal of Pharmacology, 2006Co-Authors: Daisuke Harada, Yukihito Tsukumo, Yuko Takashima, Haruhiko ManabeAbstract:The purpose of this study was to evaluate the efficacy of rolipram, a phosphodiesterase (PDE) 4 inhibitor, in a mouse model of dermatitis induced by repeated application of 2,4,6-trinitro-1-chlorobenzene (TNCB). BALB/c mice were sensitized with 0.3% w/v TNCB applied to the Ear on day -7, followed by application three times a week from day 0. Rolipram, prednisolone and cyclosporine A were administered orally once daily from day 0 to 21. Rolipram at a dose of 10 mg/kg/day significantly inhibited the Ear Thickness and the increase in cytokine levels and enzyme activity in the Ear. Interleukin (IL)-4 production was markedly decreased in cervical lymph node cells from animals treated with rolipram at a dose of 10 mg/kg/day. Prednisolone and cyclosporine A significantly reduced Ear Thickness. These compounds significantly decreased the total cell and lymphocyte number of the cervical lymph nodes. Furthermore, prednisolone markedly suppressed body weight gain, and cyclosporine A significantly increased the serum total IgE concentration compared with that in the vehicle-treated control. Rolipram, unlike prednisolone and cyclosporine A, did not influence body weight and the total IgE concentration in the serum. The present results suggest that the PDE4 inhibitor is a promising oral medicine for the treatment of chronic skin inflammatory diseases.
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Analyses of a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application
Journal of dermatological science, 2004Co-Authors: Daisuke Harada, Yukihito Tsukumo, Chie Takada, Katsumi Takaba, Haruhiko ManabeAbstract:Summary Background: The model of chronic dermatitis caused by repeated application of hapten is frequently used as a tool for assessment of the efficacy of a compound or the elucidation of chronic dermatitis. Objective: The purpose of this study is to provide more detailed analysis of the model of chronic dermatitis caused by repeated application of 2,4,6-trinitro-1-chlorobenzene (TNCB). Methods: BALB/c mice were sensitized with TNCB on day −7 to the Ear, and then TNCB was repeatedly applied to the same Ear three times per week, through days 0–21. Results: The repeated application of TNCB induced an increase of Ear Thickness, and a relatively steep increment of Ear Thickness from days 7 to 9 was observed. This increment reached almost a plateau at day 9. The peaks of Ear swelling on days 7, 14 and 21 were approximately two times higher than that on day 0. Regarding cytokines in the Ear, the highest production of IL-1β, 4, 6 and 18 were observed on day 7. The peak production of IL-1β, 4 and 6 on day 7 was found within 24 h after the challenge, while that of IL-18 was found at 0 h. The inflammatory cell infiltration into epidermis and dermis was observed and increased in day 7, then reached almost a plateau on day 9. Conclusion: The indices such as Thickness, swelling and inflammatory cell infiltration in the lesional skin was increased and maintained by repeated application, however, the protein levels of some cytokines were not always consistent with the reactions.
Corinatiulea Danciu - One of the best experts on this subject based on the ideXlab platform.
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Changes in the anti-inflammatory activity of soy isoflavonoid genistein versus genistein incorporated in two types of cyclodextrin derivatives
Chemistry Central Journal, 2012Co-Authors: Corinatiulea Danciu, Codruta Soica, Erzsébet Csányi, Rita Ambrus, Stefana Feflea, Camelia Peev, Cristina DeheleanAbstract:The isoflavonoid genistein represents the major active compound from soybean, the vegetal product from Glycine max (Fabaceae). The aim of this study is to prove that genistein was incorporated in two semisynthetic cyclodextrins, beta-cyclodextrin derivatives: hydroxypropyl-beta-cyclodextrin and randomly-methylated-beta-cyclodextrin as well as to compare the anti-inflammatory activity of genistein with that of genistein incorporated in these two types of semisynthetic cyclodextrins. The animal studies were conducted on 8-week old C57BL/6 J female mice. Inflammation was induced in both Ears of each mouse by topical application of 10 micrograms 12-O-tetradecanoylphorbol-3-acetate dissolved in 0.1 ml solvent (acetone : dimethylsulfoxide in a molar ratio 9:1). Thirty minutes later treatment was applied. The inflammatory reaction was correlated with increased values in Ear Thickness. Treatment with genistein and genistein incorporated in the two cyclodextrins led to decreased values for Ear Thickness. Better anti-inflammatory action was found for the complexes of genistein. Both haematoxylin-eosin analysis and CD45 marker expression are in agreement with these findings. Results allow concluding that genistein is an active anti-inflammatory phytocompound and its complexation with hydrophilic beta-cyclodextrin derivatives leads to a stronger anti-inflammatory activity.
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Changes in the anti-inflammatory activity of soy isoflavonoid genistein versus genistein incorporated in two types of cyclodextrin derivatives
Chemistry Central Journal, 2012Co-Authors: Corinatiulea Danciu, Codruta Soica, Erzsébet Csányi, Rita Ambrus, Stefana Feflea, Camelia Peev, Cristina DeheleanAbstract:Background The isoflavonoid genistein represents the major active compound from soybean, the vegetal product from Glycine max (Fabaceae) . The aim of this study is to prove that genistein was incorporated in two semisynthetic cyclodextrins, beta-cyclodextrin derivatives: hydroxypropyl-beta-cyclodextrin and randomly-methylated-beta-cyclodextrin as well as to compare the anti-inflammatory activity of genistein with that of genistein incorporated in these two types of semisynthetic cyclodextrins. Results The animal studies were conducted on 8-week old C57BL/6 J female mice. Inflammation was induced in both Ears of each mouse by topical application of 10 micrograms 12-O-tetradecanoylphorbol-3-acetate dissolved in 0.1 ml solvent (acetone : dimethylsulfoxide in a molar ratio 9:1). Thirty minutes later treatment was applied. The inflammatory reaction was correlated with increased values in Ear Thickness. Treatment with genistein and genistein incorporated in the two cyclodextrins led to decreased values for Ear Thickness. Better anti-inflammatory action was found for the complexes of genistein. Both haematoxylin-eosin analysis and CD45 marker expression are in agreement with these findings. Conclusions Results allow concluding that genistein is an active anti-inflammatory phytocompound and its complexation with hydrophilic beta-cyclodextrin derivatives leads to a stronger anti-inflammatory activity.
Daisuke Harada - One of the best experts on this subject based on the ideXlab platform.
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Effect of orally administered rolipram, a phosphodiesterase 4 inhibitor, on a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application
European Journal of Pharmacology, 2006Co-Authors: Daisuke Harada, Yukihito Tsukumo, Yuko Takashima, Haruhiko ManabeAbstract:The purpose of this study was to evaluate the efficacy of rolipram, a phosphodiesterase (PDE) 4 inhibitor, in a mouse model of dermatitis induced by repeated application of 2,4,6-trinitro-1-chlorobenzene (TNCB). BALB/c mice were sensitized with 0.3% w/v TNCB applied to the Ear on day -7, followed by application three times a week from day 0. Rolipram, prednisolone and cyclosporine A were administered orally once daily from day 0 to 21. Rolipram at a dose of 10 mg/kg/day significantly inhibited the Ear Thickness and the increase in cytokine levels and enzyme activity in the Ear. Interleukin (IL)-4 production was markedly decreased in cervical lymph node cells from animals treated with rolipram at a dose of 10 mg/kg/day. Prednisolone and cyclosporine A significantly reduced Ear Thickness. These compounds significantly decreased the total cell and lymphocyte number of the cervical lymph nodes. Furthermore, prednisolone markedly suppressed body weight gain, and cyclosporine A significantly increased the serum total IgE concentration compared with that in the vehicle-treated control. Rolipram, unlike prednisolone and cyclosporine A, did not influence body weight and the total IgE concentration in the serum. The present results suggest that the PDE4 inhibitor is a promising oral medicine for the treatment of chronic skin inflammatory diseases.
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Analyses of a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application
Journal of dermatological science, 2004Co-Authors: Daisuke Harada, Yukihito Tsukumo, Chie Takada, Katsumi Takaba, Haruhiko ManabeAbstract:Summary Background: The model of chronic dermatitis caused by repeated application of hapten is frequently used as a tool for assessment of the efficacy of a compound or the elucidation of chronic dermatitis. Objective: The purpose of this study is to provide more detailed analysis of the model of chronic dermatitis caused by repeated application of 2,4,6-trinitro-1-chlorobenzene (TNCB). Methods: BALB/c mice were sensitized with TNCB on day −7 to the Ear, and then TNCB was repeatedly applied to the same Ear three times per week, through days 0–21. Results: The repeated application of TNCB induced an increase of Ear Thickness, and a relatively steep increment of Ear Thickness from days 7 to 9 was observed. This increment reached almost a plateau at day 9. The peaks of Ear swelling on days 7, 14 and 21 were approximately two times higher than that on day 0. Regarding cytokines in the Ear, the highest production of IL-1β, 4, 6 and 18 were observed on day 7. The peak production of IL-1β, 4 and 6 on day 7 was found within 24 h after the challenge, while that of IL-18 was found at 0 h. The inflammatory cell infiltration into epidermis and dermis was observed and increased in day 7, then reached almost a plateau on day 9. Conclusion: The indices such as Thickness, swelling and inflammatory cell infiltration in the lesional skin was increased and maintained by repeated application, however, the protein levels of some cytokines were not always consistent with the reactions.
Camelia Peev - One of the best experts on this subject based on the ideXlab platform.
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Changes in the anti-inflammatory activity of soy isoflavonoid genistein versus genistein incorporated in two types of cyclodextrin derivatives
Chemistry Central Journal, 2012Co-Authors: Corinatiulea Danciu, Codruta Soica, Erzsébet Csányi, Rita Ambrus, Stefana Feflea, Camelia Peev, Cristina DeheleanAbstract:The isoflavonoid genistein represents the major active compound from soybean, the vegetal product from Glycine max (Fabaceae). The aim of this study is to prove that genistein was incorporated in two semisynthetic cyclodextrins, beta-cyclodextrin derivatives: hydroxypropyl-beta-cyclodextrin and randomly-methylated-beta-cyclodextrin as well as to compare the anti-inflammatory activity of genistein with that of genistein incorporated in these two types of semisynthetic cyclodextrins. The animal studies were conducted on 8-week old C57BL/6 J female mice. Inflammation was induced in both Ears of each mouse by topical application of 10 micrograms 12-O-tetradecanoylphorbol-3-acetate dissolved in 0.1 ml solvent (acetone : dimethylsulfoxide in a molar ratio 9:1). Thirty minutes later treatment was applied. The inflammatory reaction was correlated with increased values in Ear Thickness. Treatment with genistein and genistein incorporated in the two cyclodextrins led to decreased values for Ear Thickness. Better anti-inflammatory action was found for the complexes of genistein. Both haematoxylin-eosin analysis and CD45 marker expression are in agreement with these findings. Results allow concluding that genistein is an active anti-inflammatory phytocompound and its complexation with hydrophilic beta-cyclodextrin derivatives leads to a stronger anti-inflammatory activity.
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Changes in the anti-inflammatory activity of soy isoflavonoid genistein versus genistein incorporated in two types of cyclodextrin derivatives
Chemistry Central Journal, 2012Co-Authors: Corinatiulea Danciu, Codruta Soica, Erzsébet Csányi, Rita Ambrus, Stefana Feflea, Camelia Peev, Cristina DeheleanAbstract:Background The isoflavonoid genistein represents the major active compound from soybean, the vegetal product from Glycine max (Fabaceae) . The aim of this study is to prove that genistein was incorporated in two semisynthetic cyclodextrins, beta-cyclodextrin derivatives: hydroxypropyl-beta-cyclodextrin and randomly-methylated-beta-cyclodextrin as well as to compare the anti-inflammatory activity of genistein with that of genistein incorporated in these two types of semisynthetic cyclodextrins. Results The animal studies were conducted on 8-week old C57BL/6 J female mice. Inflammation was induced in both Ears of each mouse by topical application of 10 micrograms 12-O-tetradecanoylphorbol-3-acetate dissolved in 0.1 ml solvent (acetone : dimethylsulfoxide in a molar ratio 9:1). Thirty minutes later treatment was applied. The inflammatory reaction was correlated with increased values in Ear Thickness. Treatment with genistein and genistein incorporated in the two cyclodextrins led to decreased values for Ear Thickness. Better anti-inflammatory action was found for the complexes of genistein. Both haematoxylin-eosin analysis and CD45 marker expression are in agreement with these findings. Conclusions Results allow concluding that genistein is an active anti-inflammatory phytocompound and its complexation with hydrophilic beta-cyclodextrin derivatives leads to a stronger anti-inflammatory activity.
