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Š. Vaverková - One of the best experts on this subject based on the ideXlab platform.
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Gas chromatographic method for determination of uracil herbicides in roots of Echinacea angustifolia Moench (Asteraceae).
Journal of pharmaceutical and biomedical analysis, 1998Co-Authors: Jozef Tekel, Son̄a Tahotná, Š. VaverkováAbstract:Abstract A GC/NPD method and a rapid screening TLC method were developed for the simultaneous determination of uracil herbicide residues (bromacil, lenacil, terbacil) in the roots of Echinacea angustifolia Moench (Asteraceae). The uracil herbicide residues were extracted into acetone. After evaporation of acetone from the acetone-water extract the residue was dissolved in water-methanol (5:1 v/v). Cyclohexane was used for removal of the non-polar co-extractives in the sample matrix. After separation of the cyclohexane phase the uracil herbicide residues were extracted into chloroform. This extract was purified on a Florisil® column, and residues were eluted with dichloromethane-acetone (9:1, v/v). The cleaned up extract was analysed by the GC/NPD method on a capillary column DB-1 using atrazine as internal standard. A minimum recovery of 70% was attained for contamination levels of 0.02–0.40 mg kg −1 .
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Immunomodulating activity of ethanol-water extracts of the roots of Echinacea gloriosa L., Echinacea angustifolia DC. and Rudbeckia speciosa Wenderoth tested on the immune system in C57BL6 inbred mice
Ceskoslovenska farmacie, 1993Co-Authors: M Bukovský, Š. Vaverková, D Kost'álová, Magnusová RAbstract:The ethanolic extract from the roots Echinacea gloriosa L. (Moench), Echinacea angustifolia DC. and Rudbeckia speciosa Wenderoth shows immunomodulating activity. It was seen on the seventh day after five days of in vivo treatment of mice. The most marked immunostimulatory effect was observed on the lysosomal and peroxidal activity of peritoneal macrophages, and splenic cells after in vivo treatment with the ethanolic extract of the roots of R. speciosa Wenderoth.
Rudolf Bauer - One of the best experts on this subject based on the ideXlab platform.
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Alkamides from Echinacea angustifolia Interact with P-glycoprotein of primary brain capillary endothelial cells isolated from porcine brain blood vessels.
Planta medica, 2013Co-Authors: Anne Mahringer, Rudolf Bauer, Karin Ardjomand-woelkart, Gert Fricker, Thomas EfferthAbstract:The blood-brain barrier prevents the passage of toxic compounds from blood circulation into brain tissue. Unfortunately, drugs for the treatment of neurodegenerative diseases, brain tumors, and other diseases also do not cross the blood-brain barrier. In the present investigation, we used isolated porcine brain capillary endothelial cells and a flow cytometric calcein-AM assay to analyze inhibition of P-glycoprotein, a major constituent of the blood-brain barrier. We tested 8 alkamides isolated from Echinacea angustifolia and found that four of them inhibited P-glycoprotein-mediated calcein transport in porcine brain capillary endothelial cells.
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an evaluation of Echinacea angustifolia in experimental rhinovirus infections
The New England Journal of Medicine, 2005Co-Authors: Ronald B. Turner, Rudolf Bauer, Karin Woelkart, Thomas C Hulsey, David J GangemiAbstract:background Echinacea has been widely used as an herbal remedy for the common cold, but efficacy studies have produced conflicting results, and there are a variety of Echinacea products on the market with different phytochemical compositions. We evaluated the effect of chemically defined extracts from Echinacea angustifolia roots on rhinovirus infection. methods Three preparations of Echinacea, with distinct phytochemical profiles, were produced by extraction from E. angustifolia roots with supercritical carbon dioxide, 60 percent ethanol, or 20 percent ethanol. A total of 437 volunteers were randomly assigned to receive either prophylaxis (beginning seven days before the virus challenge) or treatment (beginning at the time of the challenge) either with one of these preparations or with placebo. The results for 399 volunteers who were challenged with rhinovirus type 39 and observed in a sequestered setting for five days were included in the data analysis. results There were no statistically significant effects of the three Echinacea extracts on rates of infection or severity of symptoms. Similarly, there were no significant effects of treatment on the volume of nasal secretions, on polymorphonuclear leukocyte or interleukin-8 concentrations in nasal-lavage specimens, or on quantitative-virus titer. conclusions The results of this study indicate that extracts of E. angustifolia root, either alone or in combination, do not have clinically significant effects on infection with a rhinovirus or on the clinical illness that results from it.
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The endocannabinoid system as a target for alkamides from Echinacea angustifolia roots.
Planta medica, 2005Co-Authors: Karin Woelkart, Ying Pei, Alexandros Makriyannis, Robert P. Picone, Rudolf BauerAbstract:Alkamides are the major lipophilic constituents of Echinacea angustifolia roots. Due to their structural similarity with anandamide, we have evaluated their ability to bind to rodent cannabinoid receptors CB1 and CB2 by a standard receptor binding assay using [(3)H]CP-55,940 as a radioligand. The alkamides exhibited selective affinity especially to CB2 receptors and can therefore be considered as CB ligands. Most of the alkamides showed good metabolic stability as indicated by the similarity between affinity to CB1 determined in the presence/absence of the protease inhibitor PMSF. It is suggested that CB2 interactions may be the molecular mode of action of Echinacea alkamides as immunomodulators.
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Bioavailability and pharmacokinetics of alkamides from the roots of Echinacea angustifolia in humans.
Journal of clinical pharmacology, 2005Co-Authors: Karin Woelkart, J. David Gangemi, Ronald B. Turner, Christoph Koidl, Andrea J. Grisold, Egon Marth, Rudolf BauerAbstract:Alkamides are suspected to contribute to the activity of Echinacea preparations. They are mainly derived from undeca- and dodecanoic acid and differ in the degree of unsaturation and the configuration of the double bonds. In total, 6 alkamides have been isolated from the roots of Echinacea angustifolia as major lipophilic constituents and have been investigated regarding their pharmacokinetics. A sensitive and specific method has been developed for the identification and quantification of these alkamides in human plasma using liquid chromatography electrospray ionization ion-trap mass spectrometry. The method was applied to analyze plasma samples obtained from a randomized, open, single-dose, crossover study after oral administration of a 60% ethanolic extract from the roots of E. angustifolia to 11 healthy subjects. The maximum concentration of dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides, the main alkamides in the roots of E. angustifolia, appeared already after 30 minutes and was 10.88 ng/mL for the 2.5-mL dose.
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Enzymatic degradation of echinacoside and cynarine in Echinacea angustifolia root preparations
Pharmaceutical Biology, 2004Co-Authors: Karin Wölkart, J. David Gangemi, Ronald B. Turner, Rudolf BauerAbstract:Echinacea preparations are among the bestselling herbal medicinal products in North America and Europe. Echinacoside and cynarine are the major polar constituents in the roots of Echinacea angustifolia DC and therefore are often used as marker compounds for the standardization of corresponding Echinacea preparations. This study demonstrates that echinacoside and cynarine are highly susceptible to enzymatic degradation and oxidation in hydroalcoholic solutions during the extraction process. This makes their use as analytical marker compounds still possible, although care must be taken to avoid error due to this enzymatic degradation. Batch-to-batch consistency and standardization of preparations from E. angustifolia roots require in particular a control of this enzymatic activity. The polyphenol oxidase (PPO), which is responsible for the oxidative degradation of echinacoside and cynarine, was isolated from the roots of E. angustifolia. An Electrospray Ionization-Liquid Chromotography-Mass Spectometry (ESI...
Paolo Morazzoni - One of the best experts on this subject based on the ideXlab platform.
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The effect and safety of highly standardized Ginger (Zingiber officinale) and Echinacea (Echinacea angustifolia) extract supplementation on inflammation and chronic pain in NSAIDs poor responders. A pilot study in subjects with knee arthrosis
Natural product research, 2016Co-Authors: Mariangela Rondanelli, Antonella Riva, Paolo Morazzoni, Pietro Allegrini, Milena Anna Faliva, Maurizio Naso, Alessandra Miccono, Gabriella Peroni, Irene Degli Agosti, Simone PernaAbstract:The study aimed to evaluate the effect of Zingiber officinale and Echinacea angustifolia extract supplementation (25 mg of ginger and 5 mg of Echinacea) for 30 days on inflammation and chronic pain...
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The effect and safety of highly standardized Ginger (Zingiber officinale) and Echinacea (Echinacea angustifolia) extract supplementation on inflammation and chronic pain in NSAIDs poor responders. A pilot study in subjects with knee arthrosis
2016Co-Authors: Mariangela Rondanelli, Antonella Riva, Paolo Morazzoni, Pietro Allegrini, Milena Anna Faliva, Maurizio Naso, Alessandra Miccono, Gabriella Peroni, Irene Degli Agosti, Simone PernaAbstract:The study aimed to evaluate the effect of Zingiber officinale and Echinacea angustifolia extract supplementation (25 mg of ginger and 5 mg of Echinacea) for 30 days on inflammation and chronic pain in knee osteoarthritis (OA). Consecutive nonsteroidal anti-inflammatory-drugs (NSAIDs) poor responders with chronic inflammation and pain due to knee arthrosis were assessed (15 subjects, age: 67.2 ± 7.9, body mass index: 30.6 ± 7.1, men/women:2/13). The primary endpoint was to determine pain improvement from baseline to Day 30 by Tegner Lysholm Knee Scoring. The secondary endpoints were the assessment of Visual Analog Scale for Pain, health-related quality of life, by the ShortForm36 (SF-36), anthropometric parameters, hydration. After supplementation, a significant improvement of 12.27 points was observed for Lysholm scale score (p
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In vitro and in vivo immune stimulating effects of a new standardized Echinacea angustifolia root extract (Polinacea).
Fitoterapia, 2005Co-Authors: Paolo Morazzoni, A. Cristoni, F. Di Pierro, C. Avanzini, Daniela Ravarino, Sarah Stornello, Mario Zucca, Tiziana MussoAbstract:Polinaceak is a new standardized hydroethanolic extract obtained from Echinacea angustifolia roots containing echinacoside (N4%), the high molecular weight polysaccharide IDN 5405 (N 5%) and a isobutylamide fraction (b0.1%). For in vitro tests, a bacterial lipopolysaccharide-free (LPSfree) Polinaceak has been prepared in order to avoid non-specific responses of immunocompetent cells. LPS-free Polinaceak enhanced the immune functions as highlighted by the proliferation rate and g-interferon production in murine T-lymphocyte cell cultures stimulated by anti-CD3. LPS-free Polinaceak did not have a direct role on macrophage response as measured in the nitric oxide production test using the J774 macrophage cells line. In vivo, Polinaceak showed an immune stimulating activity by reducing the Candida albicans induced mortality both in normal and in cyclosporin A-treated mice. D 2005 Elsevier B.V. All rights reserved.
Simone Perna - One of the best experts on this subject based on the ideXlab platform.
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The effect and safety of highly standardized Ginger (Zingiber officinale) and Echinacea (Echinacea angustifolia) extract supplementation on inflammation and chronic pain in NSAIDs poor responders. A pilot study in subjects with knee arthrosis
Natural product research, 2016Co-Authors: Mariangela Rondanelli, Antonella Riva, Paolo Morazzoni, Pietro Allegrini, Milena Anna Faliva, Maurizio Naso, Alessandra Miccono, Gabriella Peroni, Irene Degli Agosti, Simone PernaAbstract:The study aimed to evaluate the effect of Zingiber officinale and Echinacea angustifolia extract supplementation (25 mg of ginger and 5 mg of Echinacea) for 30 days on inflammation and chronic pain...
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The effect and safety of highly standardized Ginger (Zingiber officinale) and Echinacea (Echinacea angustifolia) extract supplementation on inflammation and chronic pain in NSAIDs poor responders. A pilot study in subjects with knee arthrosis
2016Co-Authors: Mariangela Rondanelli, Antonella Riva, Paolo Morazzoni, Pietro Allegrini, Milena Anna Faliva, Maurizio Naso, Alessandra Miccono, Gabriella Peroni, Irene Degli Agosti, Simone PernaAbstract:The study aimed to evaluate the effect of Zingiber officinale and Echinacea angustifolia extract supplementation (25 mg of ginger and 5 mg of Echinacea) for 30 days on inflammation and chronic pain in knee osteoarthritis (OA). Consecutive nonsteroidal anti-inflammatory-drugs (NSAIDs) poor responders with chronic inflammation and pain due to knee arthrosis were assessed (15 subjects, age: 67.2 ± 7.9, body mass index: 30.6 ± 7.1, men/women:2/13). The primary endpoint was to determine pain improvement from baseline to Day 30 by Tegner Lysholm Knee Scoring. The secondary endpoints were the assessment of Visual Analog Scale for Pain, health-related quality of life, by the ShortForm36 (SF-36), anthropometric parameters, hydration. After supplementation, a significant improvement of 12.27 points was observed for Lysholm scale score (p
M Bukovský - One of the best experts on this subject based on the ideXlab platform.
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Immunomodulating activity of Echinacea gloriosa L., Echinacea angustifolia DC. and Rudbeckia speciosa Wenderoth ethanol-water extracts.
Polish Journal of Pharmacology, 1995Co-Authors: M Bukovský, Vaverková S, Kostálová DAbstract:The effect of the ethanol-water extracts of Echinacea gloriosa L., Echinacea angustifolia DC. and Rudbeckia speciosa Wenderoth on immunological system of inbred mice was investigated. The extract of the root of Rudbeckia speciosa had the highest immunostimulatory activity.
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Immunomodulating activity of ethanol-water extracts of the roots of Echinacea gloriosa L., Echinacea angustifolia DC. and Rudbeckia speciosa Wenderoth tested on the immune system in C57BL6 inbred mice
Ceskoslovenska farmacie, 1993Co-Authors: M Bukovský, Š. Vaverková, D Kost'álová, Magnusová RAbstract:The ethanolic extract from the roots Echinacea gloriosa L. (Moench), Echinacea angustifolia DC. and Rudbeckia speciosa Wenderoth shows immunomodulating activity. It was seen on the seventh day after five days of in vivo treatment of mice. The most marked immunostimulatory effect was observed on the lysosomal and peroxidal activity of peritoneal macrophages, and splenic cells after in vivo treatment with the ethanolic extract of the roots of R. speciosa Wenderoth.
