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Roque Alberto Hours - One of the best experts on this subject based on the ideXlab platform.
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cultivo por lote de wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa
Revista Colombiana de Biotecnología, 2014Co-Authors: Maria Alicia Martos, Ana Paula Butiuk, Natalia Lorena Rojas, Roque Alberto HoursAbstract:Titulo en espanol: Cultivo por lote de Wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa Titulo en ingles: Batch Culture of Wickerhamomyces anomalus in a lab scale bioreactor for poligalacturonase production Titulo corto: Cultivo por lote de Wickerhamomyces anomalus Resumen: Wickerhamomyces anomalus, una levadura aislada de frutas citricas en la provincia de Misiones, Argentina, produce una poligalacturonasa (endo-PG) con capacidad macerante de tejidos vegetales. El objetivo del presente trabajo fue determinar los parametros cineticos y estequiometricos del crecimiento de W. anomalus y la produccion de la enzima poligalacturonasa en un medio de cultivo sintetico, operado en sistema por lote, en un biorreactor a escala laboratorio. Los cultivos se realizaron en un biorreactor de 4 l que contenia 3 l de un medio sintetico compuesto por glucosa, pectina de citrus, vitaminas, aminoacidos, sulfato de amonio y sales, y se incubaron con agitacion y aireacion, a 30 °C durante 12 h. El transcurso del proceso fermentativo se siguio por medidas de biomasa, glucosa residual, actividad poligalacturonasa y contenido de O 2 y CO 2 de los gases a la salida del reactor. La velocidad especifica de crecimiento maxima (μ m ) de W. anomalus fue de 0,337 h -1 y el rendimiento de biomasa producida (Y x/s ) de 0,401 g x /g s . Al finalizar el cultivo, la actividad PG en el sobrenadante fue de PG de ~ 83,7 UE/ml. La actividad especifica y la productividad obtenidas fueron de ~ 1,91. 10 4 UE/g x y ~ 9.301 UE/l.h, respectivamente. El cociente respiratorio fue cercano a 1 durante el proceso fermentativo. No se formo ningun otro producto, ademas de biomasa y CO 2 . El cultivo por lote resulto ser una buena alternativa para la produccion de PG a partir de W. anomalus , obteniendose un extracto con elevada actividad enzimatica, en un medio de cultivo sintetico y de bajo costo. Palabras clave: Wickerhamomyces anomalus , poligalacturonasa, cultivo por lote, parametros estequiometricos, parametros cineticos. Abstract: Wickerhamomyces anomalus , a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic Culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O 2 and CO 2 content of outlet gases from the reactor. The maximum specific growth rate (μ m ) of W. anomalus was 0.337 h -1 and the biomass yield (Y x/s ) was 0.40 g x /g s . At the end of the Culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/g x and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO 2 was detected. Batch Culture could be an adequate alternative for the production of polygalacturonase from W. anomalus and an extract with high enzymatic activity using a synthetic and Economic Culture medium could be obtained. Key words: Wickerhamomyces anomalus , polygalacturonase, batch Culture, kinetic parameters, stoichiometric parameters. Recibido: febrero 5 de 2014 Aprobado: octubre 20 de 2014
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cultivo por lote de wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa batch Culture of wickerhamomyces anomalus in a lab scale bioreactor for poligalacturonase production
2014Co-Authors: Maria Alicia Martos, Ana Paula Butiuk, Natalia Lorena Rojas, Roque Alberto HoursAbstract:Wickerhamomyces anomalus, a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic Culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O2 and CO2 content of outlet gases from the reactor. The maximum specific growth rate (μm) of W. anomalus was 0.337 h -1 and the biomass yield (Yx/s) was 0.40 gx/gs. At the end of the Culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/gx and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO2 was detected. Batch Culture could be an adequate alternative for the production of polygalacturonase from W. anomalus and an extract with high enzymatic activity using a synthetic and Economic Culture medium could be obtained.
Maria Alicia Martos - One of the best experts on this subject based on the ideXlab platform.
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cultivo por lote de wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa
Revista Colombiana de Biotecnología, 2014Co-Authors: Maria Alicia Martos, Ana Paula Butiuk, Natalia Lorena Rojas, Roque Alberto HoursAbstract:Titulo en espanol: Cultivo por lote de Wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa Titulo en ingles: Batch Culture of Wickerhamomyces anomalus in a lab scale bioreactor for poligalacturonase production Titulo corto: Cultivo por lote de Wickerhamomyces anomalus Resumen: Wickerhamomyces anomalus, una levadura aislada de frutas citricas en la provincia de Misiones, Argentina, produce una poligalacturonasa (endo-PG) con capacidad macerante de tejidos vegetales. El objetivo del presente trabajo fue determinar los parametros cineticos y estequiometricos del crecimiento de W. anomalus y la produccion de la enzima poligalacturonasa en un medio de cultivo sintetico, operado en sistema por lote, en un biorreactor a escala laboratorio. Los cultivos se realizaron en un biorreactor de 4 l que contenia 3 l de un medio sintetico compuesto por glucosa, pectina de citrus, vitaminas, aminoacidos, sulfato de amonio y sales, y se incubaron con agitacion y aireacion, a 30 °C durante 12 h. El transcurso del proceso fermentativo se siguio por medidas de biomasa, glucosa residual, actividad poligalacturonasa y contenido de O 2 y CO 2 de los gases a la salida del reactor. La velocidad especifica de crecimiento maxima (μ m ) de W. anomalus fue de 0,337 h -1 y el rendimiento de biomasa producida (Y x/s ) de 0,401 g x /g s . Al finalizar el cultivo, la actividad PG en el sobrenadante fue de PG de ~ 83,7 UE/ml. La actividad especifica y la productividad obtenidas fueron de ~ 1,91. 10 4 UE/g x y ~ 9.301 UE/l.h, respectivamente. El cociente respiratorio fue cercano a 1 durante el proceso fermentativo. No se formo ningun otro producto, ademas de biomasa y CO 2 . El cultivo por lote resulto ser una buena alternativa para la produccion de PG a partir de W. anomalus , obteniendose un extracto con elevada actividad enzimatica, en un medio de cultivo sintetico y de bajo costo. Palabras clave: Wickerhamomyces anomalus , poligalacturonasa, cultivo por lote, parametros estequiometricos, parametros cineticos. Abstract: Wickerhamomyces anomalus , a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic Culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O 2 and CO 2 content of outlet gases from the reactor. The maximum specific growth rate (μ m ) of W. anomalus was 0.337 h -1 and the biomass yield (Y x/s ) was 0.40 g x /g s . At the end of the Culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/g x and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO 2 was detected. Batch Culture could be an adequate alternative for the production of polygalacturonase from W. anomalus and an extract with high enzymatic activity using a synthetic and Economic Culture medium could be obtained. Key words: Wickerhamomyces anomalus , polygalacturonase, batch Culture, kinetic parameters, stoichiometric parameters. Recibido: febrero 5 de 2014 Aprobado: octubre 20 de 2014
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cultivo por lote de wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa batch Culture of wickerhamomyces anomalus in a lab scale bioreactor for poligalacturonase production
2014Co-Authors: Maria Alicia Martos, Ana Paula Butiuk, Natalia Lorena Rojas, Roque Alberto HoursAbstract:Wickerhamomyces anomalus, a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic Culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O2 and CO2 content of outlet gases from the reactor. The maximum specific growth rate (μm) of W. anomalus was 0.337 h -1 and the biomass yield (Yx/s) was 0.40 gx/gs. At the end of the Culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/gx and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO2 was detected. Batch Culture could be an adequate alternative for the production of polygalacturonase from W. anomalus and an extract with high enzymatic activity using a synthetic and Economic Culture medium could be obtained.
Joshua K Cieslewicz - One of the best experts on this subject based on the ideXlab platform.
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relationships between national Economic Culture institutions and accounting implications for ifrs
Critical Perspectives on Accounting, 2014Co-Authors: Joshua K CieslewiczAbstract:National Economic Culture has an indirect influence on accounting at the national level through the mediating variable of institutions. The relationships are evaluated using measures of national Economic Culture from Culture, Leadership, and Organizations: The GLOBE Study of 62 Societies (House et al., 2004); measures of institutions from the World Bank (Kaufmann et al., 2007); and measures of national accounting from the Financial Standards Foundation (2008). At the national level, institutions are found to mediate the relationship between Economic Culture variables and accounting. The empirical findings indicate that accounting in a given nation is linked to the nation's supporting institutions, which institutions in turn are influenced by the national Economic Culture of those who maintain them. This suggests that altering aspects of accounting within a nation can be expected to entail much more than formal adoption of standards, principles, or innovations. Institutional adjustments must be made and the impact of national Economic Culture must be understood and appropriately addressed.
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the impact of national Economic Culture and country level institutional environment on corporate governance practices
Management International Review, 2012Co-Authors: Shirley J Daniel, Joshua K Cieslewicz, Hamid PourjalaliAbstract:We posit that national cultural practices influence the institutional environment, which in turn has an influence on corporate governance practices. We empirically evaluate these relationships using structural equation modeling. We utilize measures of national Culture from Culture, Leadership, and Organizations: The Globe study of 62 Societies. For the institutional environment, we employ the World Bank Worldwide Governance Indicators. Consistent with theory, we find a strong relationship between these constructs. We further find that the institutional environment mediates the relationship between national Culture and corporate governance practices. Corporate governance practices include measures of board accountability, financial disclosure and internal controls, shareholder rights, executive compensation, takeover defenses and ownership base, and corporate accountability and are available from Governance Metrics International. As both Culture and institutions are linked to corporate governance practices, efforts to change corporate governance practices around the world are best informed by an appreciation of cultural as well as institutional factors.
Ana Paula Butiuk - One of the best experts on this subject based on the ideXlab platform.
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cultivo por lote de wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa
Revista Colombiana de Biotecnología, 2014Co-Authors: Maria Alicia Martos, Ana Paula Butiuk, Natalia Lorena Rojas, Roque Alberto HoursAbstract:Titulo en espanol: Cultivo por lote de Wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa Titulo en ingles: Batch Culture of Wickerhamomyces anomalus in a lab scale bioreactor for poligalacturonase production Titulo corto: Cultivo por lote de Wickerhamomyces anomalus Resumen: Wickerhamomyces anomalus, una levadura aislada de frutas citricas en la provincia de Misiones, Argentina, produce una poligalacturonasa (endo-PG) con capacidad macerante de tejidos vegetales. El objetivo del presente trabajo fue determinar los parametros cineticos y estequiometricos del crecimiento de W. anomalus y la produccion de la enzima poligalacturonasa en un medio de cultivo sintetico, operado en sistema por lote, en un biorreactor a escala laboratorio. Los cultivos se realizaron en un biorreactor de 4 l que contenia 3 l de un medio sintetico compuesto por glucosa, pectina de citrus, vitaminas, aminoacidos, sulfato de amonio y sales, y se incubaron con agitacion y aireacion, a 30 °C durante 12 h. El transcurso del proceso fermentativo se siguio por medidas de biomasa, glucosa residual, actividad poligalacturonasa y contenido de O 2 y CO 2 de los gases a la salida del reactor. La velocidad especifica de crecimiento maxima (μ m ) de W. anomalus fue de 0,337 h -1 y el rendimiento de biomasa producida (Y x/s ) de 0,401 g x /g s . Al finalizar el cultivo, la actividad PG en el sobrenadante fue de PG de ~ 83,7 UE/ml. La actividad especifica y la productividad obtenidas fueron de ~ 1,91. 10 4 UE/g x y ~ 9.301 UE/l.h, respectivamente. El cociente respiratorio fue cercano a 1 durante el proceso fermentativo. No se formo ningun otro producto, ademas de biomasa y CO 2 . El cultivo por lote resulto ser una buena alternativa para la produccion de PG a partir de W. anomalus , obteniendose un extracto con elevada actividad enzimatica, en un medio de cultivo sintetico y de bajo costo. Palabras clave: Wickerhamomyces anomalus , poligalacturonasa, cultivo por lote, parametros estequiometricos, parametros cineticos. Abstract: Wickerhamomyces anomalus , a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic Culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O 2 and CO 2 content of outlet gases from the reactor. The maximum specific growth rate (μ m ) of W. anomalus was 0.337 h -1 and the biomass yield (Y x/s ) was 0.40 g x /g s . At the end of the Culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/g x and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO 2 was detected. Batch Culture could be an adequate alternative for the production of polygalacturonase from W. anomalus and an extract with high enzymatic activity using a synthetic and Economic Culture medium could be obtained. Key words: Wickerhamomyces anomalus , polygalacturonase, batch Culture, kinetic parameters, stoichiometric parameters. Recibido: febrero 5 de 2014 Aprobado: octubre 20 de 2014
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cultivo por lote de wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa batch Culture of wickerhamomyces anomalus in a lab scale bioreactor for poligalacturonase production
2014Co-Authors: Maria Alicia Martos, Ana Paula Butiuk, Natalia Lorena Rojas, Roque Alberto HoursAbstract:Wickerhamomyces anomalus, a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic Culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O2 and CO2 content of outlet gases from the reactor. The maximum specific growth rate (μm) of W. anomalus was 0.337 h -1 and the biomass yield (Yx/s) was 0.40 gx/gs. At the end of the Culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/gx and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO2 was detected. Batch Culture could be an adequate alternative for the production of polygalacturonase from W. anomalus and an extract with high enzymatic activity using a synthetic and Economic Culture medium could be obtained.
Natalia Lorena Rojas - One of the best experts on this subject based on the ideXlab platform.
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cultivo por lote de wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa
Revista Colombiana de Biotecnología, 2014Co-Authors: Maria Alicia Martos, Ana Paula Butiuk, Natalia Lorena Rojas, Roque Alberto HoursAbstract:Titulo en espanol: Cultivo por lote de Wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa Titulo en ingles: Batch Culture of Wickerhamomyces anomalus in a lab scale bioreactor for poligalacturonase production Titulo corto: Cultivo por lote de Wickerhamomyces anomalus Resumen: Wickerhamomyces anomalus, una levadura aislada de frutas citricas en la provincia de Misiones, Argentina, produce una poligalacturonasa (endo-PG) con capacidad macerante de tejidos vegetales. El objetivo del presente trabajo fue determinar los parametros cineticos y estequiometricos del crecimiento de W. anomalus y la produccion de la enzima poligalacturonasa en un medio de cultivo sintetico, operado en sistema por lote, en un biorreactor a escala laboratorio. Los cultivos se realizaron en un biorreactor de 4 l que contenia 3 l de un medio sintetico compuesto por glucosa, pectina de citrus, vitaminas, aminoacidos, sulfato de amonio y sales, y se incubaron con agitacion y aireacion, a 30 °C durante 12 h. El transcurso del proceso fermentativo se siguio por medidas de biomasa, glucosa residual, actividad poligalacturonasa y contenido de O 2 y CO 2 de los gases a la salida del reactor. La velocidad especifica de crecimiento maxima (μ m ) de W. anomalus fue de 0,337 h -1 y el rendimiento de biomasa producida (Y x/s ) de 0,401 g x /g s . Al finalizar el cultivo, la actividad PG en el sobrenadante fue de PG de ~ 83,7 UE/ml. La actividad especifica y la productividad obtenidas fueron de ~ 1,91. 10 4 UE/g x y ~ 9.301 UE/l.h, respectivamente. El cociente respiratorio fue cercano a 1 durante el proceso fermentativo. No se formo ningun otro producto, ademas de biomasa y CO 2 . El cultivo por lote resulto ser una buena alternativa para la produccion de PG a partir de W. anomalus , obteniendose un extracto con elevada actividad enzimatica, en un medio de cultivo sintetico y de bajo costo. Palabras clave: Wickerhamomyces anomalus , poligalacturonasa, cultivo por lote, parametros estequiometricos, parametros cineticos. Abstract: Wickerhamomyces anomalus , a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic Culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O 2 and CO 2 content of outlet gases from the reactor. The maximum specific growth rate (μ m ) of W. anomalus was 0.337 h -1 and the biomass yield (Y x/s ) was 0.40 g x /g s . At the end of the Culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/g x and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO 2 was detected. Batch Culture could be an adequate alternative for the production of polygalacturonase from W. anomalus and an extract with high enzymatic activity using a synthetic and Economic Culture medium could be obtained. Key words: Wickerhamomyces anomalus , polygalacturonase, batch Culture, kinetic parameters, stoichiometric parameters. Recibido: febrero 5 de 2014 Aprobado: octubre 20 de 2014
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cultivo por lote de wickerhamomyces anomalus en un biorreactor a escala laboratorio para la produccion de una poligalacturonasa batch Culture of wickerhamomyces anomalus in a lab scale bioreactor for poligalacturonase production
2014Co-Authors: Maria Alicia Martos, Ana Paula Butiuk, Natalia Lorena Rojas, Roque Alberto HoursAbstract:Wickerhamomyces anomalus, a yeast isolated from citrus fruit peels in the province of Misiones, Argentina, produces a polygalacturonase (endo-PG) with maceration activity of vegetable tissues. The objective of the present work was to determine kinetic and stoichiometric parameters of W. anomalus growth and polygalacturonase production in a synthetic Culture medium, operating in a batch-type bioreactor at laboratory scale. Cultures were performed in a bioreactor of 4 l, containing 3 l of a synthetic medium composed of glucose, citrus pectin, vitamins, amino acids, ammonium sulfate and salts, and were incubated with agitation (450 rpm) and aeration at 30 °C, during 12 h. The course of the fermentation process was followed by measuring biomass, residual glucose, polygalacturonase activity and O2 and CO2 content of outlet gases from the reactor. The maximum specific growth rate (μm) of W. anomalus was 0.337 h -1 and the biomass yield (Yx/s) was 0.40 gx/gs. At the end of the Culture, PG activity in the supernatant was ~84 UE/ml. The specific activity and the productivity obtained were ~1.91 10 4 UE/gx and ~9,301 UE/l.h, respectively. Respiratory quotient was approximately 1.0 throughout the fermentation process. No other product different from biomass and CO2 was detected. Batch Culture could be an adequate alternative for the production of polygalacturonase from W. anomalus and an extract with high enzymatic activity using a synthetic and Economic Culture medium could be obtained.
