Wickerhamomyces

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Guido Favia - One of the best experts on this subject based on the ideXlab platform.

  • Wickerhamomyces anomalus in mosquitoes a promising yeast based tool for the symbiotic control of mosquito borne diseases
    Frontiers in Microbiology, 2021
    Co-Authors: Alessia Cappelli, Guido Favia, Irene Ricci
    Abstract:

    The ascomycete yeast Wickerhamomyces anomalus is a mutualistic symbiont of different insects, including diptera vectors of diseases. Although fungal symbioses have been so far poorly characterized, the topic is gaining attention as yeast-insect interactions can provide pivotal information on insect biology, such as their environmental adaptation or vectorial capability. We review the symbiosis between W. anomalus and mosquitoes, which implies nutritional and protective functions. Furthermore, we focus on antiplasmodial effects of W. anomalus in malaria vectors and discuss the yeast potential for the "symbiotic control" (SC) of mosquito-borne diseases (MBDs).

  • Identification of a Killer Toxin from Wickerhamomyces anomalus with β-Glucanase Activity.
    Toxins, 2019
    Co-Authors: Valentina Cecarini, Massimiliano Cuccioloni, Laura Bonfili, Massimo Ricciutelli, Consuelo Amantini, Guido Favia, Anna Maria Eleuteri, Mauro Angeletti
    Abstract:

    The yeast Wickerhamomyces anomalus has several applications in the food industry due to its antimicrobial potential and wide range of biotechnological properties. In particular, a specific strain of Wickerhamomyces anomalus isolated from the malaria mosquito Anopheles stephensi, namely WaF17.12, was reported to secrete a killer toxin with strong anti-plasmodial effect on different developmental stages of Plasmodium berghei; therefore, we propose its use in the symbiotic control of malaria. In this study, we focused on the identification/characterization of the protein toxin responsible for the observed antimicrobial activity of the yeast. For this purpose, the culture medium of the killer yeast strain WaF17.12 was processed by means of lateral flow filtration, anion exchange and gel filtration chromatography, immunometric methods, and eventually analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Based on this concerted approach, we identified a protein with a molecular weight of approximately 140 kDa and limited electrophoretic mobility, corresponding to a high molecular weight β-glucosidase, as confirmed by activity tests in the presence of specific inhibitors.

  • Isolation of a Wickerhamomyces anomalus yeast strain from the sandfly Phlebotomus perniciosus, displaying the killer phenotype
    Medical and Veterinary Entomology, 2015
    Co-Authors: Elena Martin, Laura Giovati, Gioia Bongiorno, Matteo Montagna, E.e Crotti, C.f Damiani, Luigi Gradoni, Luciano Polonelli, Guido Favia
    Abstract:

    The yeast Wickerhamomyces anomalus has been studied for its wide biotechnological potential, mainly for applications in the food industry. Different strains of W. anomalus have been isolated from diverse habitats and recently from insects, including mosquitoes of medical importance. This paper reports the isolation and phylogenetic characterization of W. anomalus from laboratory-reared adults and larvae of Phlebotomus perniciosus (Diptera: Psychodidae), a main phlebotomine vector of human and canine leishmaniasis. Of 65 yeast strains isolated from P. perniciosus, 15 strains were identified as W. anomalus; one of these was tested for the killer phenotype and demonstrated inhibitory activity against four yeast sensitive strains, as reported for mosquito-isolated strains. The association between P. perniciosus and W. anomalus deserves further investigation in order to explore the possibility that this yeast may exert inhibitory/killing activity against Leishmania spp.

  • A rapid qPCR method to investigate the circulation of the yeast Wickerhamomyces anomalus in humans.
    The new microbiologica, 2015
    Co-Authors: Sara Epis, Elena Martin, Michela Paolucci, Chiara Bazzocchi, Jovana Bozic, Stefano Novati, Guido Favia
    Abstract:

    The yeast Wickerhamomyces anomalus has been proposed for many biotechnological applications in the food industry. However, a number of opportunistic pathogenic strains have been reported as causative agents of nosocomial fungemia. Recognition of potentially pathogenic isolates is an important challenge for the future commercialization of this yeast. The isolation of W. anomalus from different matrices and, recently, from mosquitoes, requires further investigations into its circulation in humans. Here we present a qPCR protocol for the detection of W. anomalus in human blood samples and the results of a screening of 525 donors, including different classes of patients and healthy people.

  • A Wickerhamomyces anomalus Killer Strain in the Malaria Vector Anopheles stephensi
    PLoS ONE, 2014
    Co-Authors: Sara Epis, Claudio Bandi, Stefania Conti, Luciano Polonelli, Maria Gabriella Gabrielli, Guido Favia
    Abstract:

    The yeast Wickerhamomyces anomalus has been investigated for several years for its wide biotechnological potential, especially for applications in the food industry. Specifically, the antimicrobial activity of this yeast, associated with the production of Killer Toxins (KTs), has attracted a great deal of attention. The strains of W. anomalus able to produce KTs, called “killer” yeasts, have been shown to be highly competitive in the environment. Different W. anomalus strains have been isolated from diverse habitats and recently even from insects. In the malaria mosquito vector Anopheles stephensi these yeasts have been detected in the midgut and gonads. Here we show that the strain of W. anomalus isolated from An. stephensi, namely WaF17.12, is a killer yeast able to produce a KT in a cell-free medium (in vitro) as well as in the mosquito body (in vivo). We showed a constant production of WaF17.12-KT over time, after stimulation of toxin secretion in yeast cultures and reintroduction of the activated cells into the mosquito through the diet. Furthermore, the antimicrobial activity of WaF17.12-KT has been demonstrated in vitro against sensitive microbes, showing that strain WaF17.12 releases a functional toxin. The mosquito-associated yeast WaF17.12 thus possesses an antimicrobial activity, which makes this yeast worthy of further investigations, in view of its potential as an agent for the symbiotic control of malaria.

Hiroko Kawasaki - One of the best experts on this subject based on the ideXlab platform.

  • three novel species of d xylose assimilating yeasts barnettozyma xylosiphila sp nov barnettozyma xylosica sp nov and Wickerhamomyces xylosivorus f a sp nov
    International Journal of Systematic and Evolutionary Microbiology, 2017
    Co-Authors: Ryuichi Kobayashi, Atit Kanti, Hiroko Kawasaki
    Abstract:

    This study describes three novel xylose-assimilating yeasts, which were isolated from decayed wood collected from Bung Hatta Botanical Garden in West Sumatra and Cibodas Botanic Garden in West Java, or from litter from Eka Karya Bali Botanic Garden in Bali, Indonesia. Phylogenetic analysis was performed based on the sequences of the D1/D2 domains of the large ribosomal subunit (LSU), the small ribosomal subunit (SSU), the internal transcribed spacer (ITS) and elongation factor-1α (EF-1α), and the three strains were found to represent three novel species belonging to genera Barnettozyma or Wickerhamomyces. The morphological, biochemical and physiological characteristics indicated that the strains were distinct from other closely related species. Strains 13Y206T and 14Y196T belonging to the Barnettozyma clade are described as the type strains of Barnettozyma xylosiphila sp. nov. (type strain 13Y206T=NBRC 110202T=InaCC Y726T; MycoBank MB808598) and Barnettozyma xylosica sp. nov. (type strain 14Y196T=NBRC 111558T=InaCC Y1030T; MycoBank MB819485). Strain 14Y125T belonging to the Wickerhamomyces clade is described as the type strain of Wickerhamomyces xylosivorus f.a., sp. nov. (type strain 14Y125T=NBRC 111553T=InaCC Y1026T; MycoBank MB819484).

  • two novel ascomycetous yeast species Wickerhamomyces scolytoplatypi sp nov and cyberlindnera xylebori sp nov isolated from ambrosia beetle galleries
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Shinya Ninomiya, Kozaburo Mikata, Hisashi Kajimura, Hiroko Kawasaki
    Abstract:

    Thirteen strains of yeasts were isolated from ambrosia beetle galleries at several sites in Japan. Based on the morphological and biochemical characteristics and phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene of the yeasts, 10 strains were shown to represent a novel species of the genus Wickerhamomyces, described as Wickerhamomyces scolytoplatypi sp. nov. (type strain NBRC 11029(T) = CBS 12186(T)), and were closely related to Wickerhamomyces hampshirensis. The three other strains represented a novel species of the genus Cyberlindnera, described as Cyberlindnera xylebori sp. nov. (type strain NBRC 11048(T) = CBS 12187(T)), and were closely related to Cyberlindnera euphorbiiphila. It is suggested that these species are associated with ambrosia beetles and we consider ambrosia beetle galleries as good sources of novel yeasts.

  • Wickerhamomyces siamensis sp nov a novel yeast species isolated from the phylloplane in thailand
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Rungluk Kaewwichian, Hiroko Kawasaki, Savitree Limtong
    Abstract:

    Strain DMKU-RK359T, representing a novel yeast species, was isolated from the external surface of a sugar-cane leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 region of the large-subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK359T was assigned to a novel Wickerhamomyces species. The novel species was closest to Wickerhamomyces ciferrii, but differed from it by 0.7 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and 6 % nucleotide substitutions in the ITS region. The name Wickerhamomyces siamensis sp. nov. is proposed (type strain DMKU-RK359T  = BCC 50732T  = NBRC 108900T  = CBS 12570T).

  • Wickerhamomyces tratensis sp nov and candida namnaoensis sp nov two novel ascomycetous yeast species in the Wickerhamomyces clade found in thailand
    Journal of General and Applied Microbiology, 2012
    Co-Authors: Sasitorn Jindamorakot, Takashi Nakase, Shinya Ninomiya, Somjit Amin, Hiroko Kawasaki
    Abstract:

    Two closely related yeast strains, ST-382 and ST-392, isolated in Thailand showed intermediate relatedness in the DNA-DNA hybridization experiment suggesting that the two strains represent closely related distinct species. In the tree based on the D1/D2 domain sequences of the large subunit rRNA gene, the two strains are located in a subclade in the Wickerhamomyces clade with high bootstrap support. In the D1/D2 domain, the two strains differed by two nucleotides and are assumed to be very closely related. Strain ST-392(T) (=BCC 15102(T) = NBRC 107799(T) = CBS 12176(T) forming hat-shaped ascospores is described as Wickerhamomyces tratensis sp. nov. and strain ST-382(T) (= BCC 15093(T) = NBRC 107800(T) = CBS 12175(T) is described as Candida namnaoensis sp. nov. because ascospores are not found in this strain. In phenotypic characteristics, W. tratensis and C. namnaoensis are discriminated by the ability of alcoholic fermentation and the assimilation of galactose, D-xylose and D-gluconic acid.

  • Wickerhamomyces edaphicus sp nov and pichia jaroonii sp nov two ascomycetous yeast species isolated from forest soil in thailand
    Fems Yeast Research, 2009
    Co-Authors: Savitree Limtong, Hiroko Kawasaki, Wichien Yongmanitchai, Kazuhito Fujiyama
    Abstract:

    Four yeast strains were isolated from soil in a mixed deciduous forest in Amphoe Wang Nam Khiao, Nakhon Ratchasima province, Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analyses of the D1/D2 domain of the large-subunit (LSU) rRNA gene, small-subunit rRNA gene and internal transcribed spacer (ITS) region, the three strains (S-29, S-63 and S-80) were found to represent a single species of the genus Wickerhamomyces, which were named Wickerhamomyces edaphicus sp. nov. The type strain is S-29 T (BCC 21231 T = NBRC 101969 T = CBS 10408 T ). Strain S-75 represented a novel species of the genus Pichia on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analyses of the D1/D2 domain of the LSU rRNA gene, for which the name Pichia jaroonii sp. nov. is proposed. The type strain is S-75 T (BCC 23061 T , NRBC 102180 T = CBS 10930 T ).

Savitree Limtong - One of the best experts on this subject based on the ideXlab platform.

  • Wickerhamomyces siamensis sp nov a novel yeast species isolated from the phylloplane in thailand
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Rungluk Kaewwichian, Hiroko Kawasaki, Savitree Limtong
    Abstract:

    Strain DMKU-RK359T, representing a novel yeast species, was isolated from the external surface of a sugar-cane leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 region of the large-subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK359T was assigned to a novel Wickerhamomyces species. The novel species was closest to Wickerhamomyces ciferrii, but differed from it by 0.7 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and 6 % nucleotide substitutions in the ITS region. The name Wickerhamomyces siamensis sp. nov. is proposed (type strain DMKU-RK359T  = BCC 50732T  = NBRC 108900T  = CBS 12570T).

  • Wickerhamomyces xylosica sp nov and candida phayaonensis sp nov two xylose assimilating yeast species from soil
    International Journal of Systematic and Evolutionary Microbiology, 2012
    Co-Authors: Savitree Limtong, Sasitorn Jindamorakot, Rungluk Kaewwichian, Sukanya Nitiyon, Somjit Amin, Wichien Yongmanitchai
    Abstract:

    Two strains (NT29(T) and NT31(T)) of xylose-assimilating yeasts were obtained from soils collected in northern Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer region, the two strains were found to represent two novel ascomycete yeast species. Strain NT29(T) was assigned to the genus Candida belonging to the Pichia clade as a representative of Candida phayaonensis sp. nov.; the type strain is NT29(T) (=BCC 47634(T)=NBRC 108868(T)=CBS 12319(T)). Strain NT31(T) represented a novel Wickerhamomyces species, which was named Wickerhamomyces xylosica sp. nov.; the type strain is NT31(T) (=BCC 47635(T)=NBRC 108869(T)=CBS 12320(T)).

  • mode of vegetative reproduction of the bipolar budding yeast species Wickerhamomyces pijperi and related strains
    Microbiology, 2009
    Co-Authors: Yumi Imanishi, Sasitorn Jindamorakot, Savitree Limtong, Takashi Nakase
    Abstract:

    To clarify the budding pattern of Wickerhamomyces pijperi, the vegetative cells were observed by scanning electron microscopy. The cells grew by bipolar budding, but cells that budded from the shoulder of a mother cell were occasionally observed. We examined the cell morphology and phylogeny of five strains of Wickerhamomyces sp. isolated in Thailand as well as seven W. pijperi and three Wickerhamomyces sp. strains that were preserved in culture collections. Phylogenetic analysis based on three different nucleotide sequences (D1/D2 domain of 26S rDNA, the actin gene ACT1 and the elongation factor 2 gene EF2) indicated that all the strains belonged to the genus Wickerhamomyces and were neighbours of the type strain W. pijperi NBRC 1290T. The strains fell into two groups in this analysis. The budding patterns of the strains were carefully observed by staining the bud scars, and these patterns were categorized into three groups: types I–III. Type I included cells that grew by bipolar budding and formed multiple scars, type III included cells that grew by multilateral budding and formed a single scar, and type II included cells that exhibited a mixture of type I and type III patterns. Among the 15 strains, 12 strains, including W. pijperi NBRC 1290T, mainly exhibited type I or type II budding patterns; these strains belonged to group 1 of the phylogenetic analysis. The remaining three strains, which belonged to group 2, exhibited either type II or type III patterns. Thus the phylogenetic relationship and budding patterns are related. Moreover, some cells also exhibited budding characteristics that were intermediate between bipolar and multilateral budding.

  • Wickerhamomyces edaphicus sp nov and pichia jaroonii sp nov two ascomycetous yeast species isolated from forest soil in thailand
    Fems Yeast Research, 2009
    Co-Authors: Savitree Limtong, Hiroko Kawasaki, Wichien Yongmanitchai, Kazuhito Fujiyama
    Abstract:

    Four yeast strains were isolated from soil in a mixed deciduous forest in Amphoe Wang Nam Khiao, Nakhon Ratchasima province, Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analyses of the D1/D2 domain of the large-subunit (LSU) rRNA gene, small-subunit rRNA gene and internal transcribed spacer (ITS) region, the three strains (S-29, S-63 and S-80) were found to represent a single species of the genus Wickerhamomyces, which were named Wickerhamomyces edaphicus sp. nov. The type strain is S-29 T (BCC 21231 T = NBRC 101969 T = CBS 10408 T ). Strain S-75 represented a novel species of the genus Pichia on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analyses of the D1/D2 domain of the LSU rRNA gene, for which the name Pichia jaroonii sp. nov. is proposed. The type strain is S-75 T (BCC 23061 T , NRBC 102180 T = CBS 10930 T ).

Carlos A Rosa - One of the best experts on this subject based on the ideXlab platform.

  • second generation ethanol from non detoxified sugarcane hydrolysate by a rotting wood isolated yeast strain
    Bioresource Technology, 2017
    Co-Authors: Suzana F Bazoti, Carlos A Rosa, Simone Maria Golunski, Diego Pereira Siqueira, Thamarys Scapini, Evelyn Taize Barrilli, Diego A Mayer, Katharina O Barros, Boris U Stambuk, Sergio L Alves
    Abstract:

    This work aims to evaluate the production of second-generation ethanol from sugarcane bagasse hydrolysate without acetic acid (inhibitor) detoxification. Three isolated yeast strains from lignocellulosic materials were evaluated, and one strain (UFFS-CE-3.1.2), identified using large subunit rDNA sequences as Wickerhamomyces sp., showed satisfactory results in terms of ethanol production without acetic acid removal. A Plackett-Burman design was used to evaluate the influence of hydrolysate composition and nutrients supplementation in the fermentation medium for the second-generation ethanol production. Two fermentation kinetics were performed, with controlled pH at 5.5, or keeping the initial pH at 4.88. The fermentation conducted without pH adjustment and supplementation of nutrients reported the best result in terms of second-generation ethanol production. Wickerhamomyces sp., isolated as UFFS-CE-3.1.2, was considered promising in the production of second-generation ethanol by using crude (non-detoxified) sugarcane hydrolysate.

  • Wickerhamomyces patagonicus sp nov an ascomycetous yeast species from patagonia argentina
    International Journal of Systematic and Evolutionary Microbiology, 2010
    Co-Authors: Virginia De Garcia, Carlos A Rosa, Silvia Brizzio, Diego Libkind, Maria Van Broock
    Abstract:

    Eight strains of a novel yeast species were isolated from tree saps of ‘Coihue’ (Nothofagus dombeyi, Nothofagaceae) and glacial meltwater (Castano Overo River) in the Nahuel Huapi National Park, Patagonia, Argentina. The sequences of the D1/D2 domains of the large subunit of the rRNA gene showed that this novel yeast species belongs to the Wickerhamomyces genus (Order Saccharomycetales, Family Wickerhamomycetaceae). The closest related species were Candida ponderosae and Wickerhamomyces chambardii. Wickerhamomyces patagonicus sp. nov. is proposed to accommodate these novel strains, with the type strain CRUB 1724T (=CBS 11398T =JCM 16381T).

  • Wickerhamomyces queroliae sp nov and candida jalapaonensis sp nov two yeast species isolated from cerrado ecosystem in north brazil
    International Journal of Systematic and Evolutionary Microbiology, 2009
    Co-Authors: Carlos A Rosa, Paula B Morais, Marcandre Lachance, Renata O Santos, Weilan G P Melo, Rodney Haulien Oliveira Viana, Marcos A L Braganca, Raphael Sanzio Pimenta
    Abstract:

    Two novel yeast species, Wickerhamomyces queroliae sp. nov. and Candida jalapaonensis sp. nov., were isolated, respectively, from larvae of Anastrepha mucronata (Diptera: Tephritidae) collected from ripe fruit of Peritassa campestris (‘Bacupari’, Hippocrateaceae) and from flowers of Centropogon cornutus (Campanulaceae) in the Cerrado ecosystem of the state of Tocantins, Brazil. Analysis of the D1/D2 large-subunit rRNA gene sequences placed W. queroliae in the Wickerhamomyces clade near Wickerhamomyces ciferri and Candida silvicultrix. Candida jalapaonensis belongs to the Wickerhamiella clade and is related to Candida drosophilae. The type strain of Wickerhamomyces queroliae is UFMG-05-T200.1T (=CBS 10936T=NRRL Y-48478T) and the type strain of Candida jalapaonensis is UFMG-03-T210T (=CBS 10935T=NRRL Y-48477T).

Disney Ribeiro Dias - One of the best experts on this subject based on the ideXlab platform.

  • New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358.
    Colloids and Surfaces B: Biointerfaces, 2017
    Co-Authors: Karla Silva Teixeira Souza, Eduardo J Gudina, Rosane Freitas Schwan, Disney Ribeiro Dias, Zélia Azevedo, Victor De Freitas, Lígia R. Rodrigues, José A. Teixeira
    Abstract:

    In this work, biosurfactant production by several yeast strains was evaluated using different culture media. The best results were obtained with the strain Wickerhamomyces anomalus CCMA 0358 growing in a culture medium containing glucose (1g/L) and olive oil (20g/L) as carbon sources. This strain produced 2.6g of biosurfactant per liter after 24h of growth. The crude biosurfactant reduced the surface tension of water to values around 31mN/m, and its critical micelle concentration was 0.9mg/mL. This biosurfactant was characterized through mass spectrometry (MS), and nuclear magnetic resonance (NMR) as a mixture of two different glycolipids, comprising a sugar moiety linked to one or three molecules of oleic acid. To the best of our knowledge, these biosurfactants are structurally different from those previously reported. Furthermore, the crude biosurfactant exhibited antimicrobial activity against several microorganisms, including the pathogens Candida albicans, Escherichia coli, Staphylococcus epidermidis and Streptococcus agalactiae, which opens the possibility for its use in several biomedical applications.

  • Eco-friendly biosurfactant from Wickerhamomyces anomalus CCMA 0358 as larvicidal and antimicrobial
    Microbiological Research, 2024
    Co-Authors: Natalia De Andrade Teixeira Fernandes, Angélica Cristina De Souza, Luara Aparecida Simões, Gustavo Magno Ferreira Dos Reis, Karla Silva Teixeira Souza, Rosane Freitas Schwan, Disney Ribeiro Dias
    Abstract:

    Kitchen waste oil (KWO) was evaluated as a substrate for production of biosurfactant by Wickerhamomyces anomalus CCMA 0358 and was tested against Aedes aegypti larvae, the mosquito causing neglected diseases, such as dengue fever, Zika, and Chikungunya, achieving 100 % mortality in the lowest concentration (6.25 %) evaluated in 24 h. Furthermore, possible applications of this compound were evaluated as antibacterial, antiadhesive, and antifungal. At a concentration of 50 %, the biosurfactant was found to inhibit the growth of Bacillus cereus, showing high inhibitions levels against Salmonella Enteritidis, Staphylococcus aureus, and Escherichia coli. The antifungal activity was evaluated against Aspergillus, Cercospora, Colletotrichum, and Fusarium, obtaining results of up to 95 % inhibition. In addition to these promising results, the yeast W. anomalus produced the biosurfactant from an inexpensive substrate, which increases the possibility of its application in several industries owing to the low cost involved.

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