The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
Bao-jun Chen - One of the best experts on this subject based on the ideXlab platform.
-
Immuno-protective effect of Tumor cell vaccine on Kunming mice bearing Ehrlich Ascites Tumor.
World Journal of Gastroenterology, 1998Co-Authors: Zheng Ma, Shao-juan Zhou, Kaichun Wu, Taidong Qiao, Bao-jun ChenAbstract:AIM: To evaluate the immunity of chemically modified Tumor cell vaccine. METHODS: Tumor cell vaccines (TCV) were prepared by incubating the live Ehrlich Ascites Tumor cells with concanavalin A-mitomycin C (ConA-MMC), mitomycin C (MMC), concanavalin A-glutaraldehyde (ConA-Glu), glutaraldehyde ( Glu ), or paraformaldehyde ( Para ), respectively. The whole cell or soluble forms of the vaccines were administered intraperitoneally into Kunming mice once a week for three times prior to the intraperitoneal inoculation of a lethal dose of live Tumor cells. A second challenge with live Tumor cells was given four weeks later. Survival and antibody production of the mice were analyzed. RESULTS: After the first challenge, the mice, received whole TCV of ConA-MMC, MMC (P < 0.01) and Glu (P < 0.05) promoted survival incidence than the controls. All the treated mice had the survival time prolonged. ConA-MMC vaccine treated mice had longer survival days than that of ConA-Glu ones (P < 0.05). For the soluble TCV immunized mice, those treated with vaccines of Para (P < 0.01), ConA-Para and ConA-Glu (P < 0.05) had longer survival periods compared with that of the controls. Following the second challenge, survival incidence of the mice received vaccines of ConA-MMC, MMC, ConA-Glu or Glu was significantly increased (P < 0.01). Moreover, all the treated mice had the survival time prolonged, and ConA-MMC vaccine treated mice had longer survival days than that of Para treated ones (P < 0.05). Antibodies against Ehrlich Ascites Tumor cells were found to be positive in sera of the mice treated with whole TCV of ConA-MMC. CONCLUSION: Ehrlich Ascites Tumor cells are immunogenic when treated with ConA-MMC, MMC, ConA-Glu, Glu or Para, which might act as safe and effective Tumor vaccines with safety and effectiveness.
Fan Dai - One of the best experts on this subject based on the ideXlab platform.
-
Tumor-specific cytotoxicity induced by Ehrlich Ascites Tumor cell vaccine
Journal of Cellular and Molecular Immunology, 2001Co-Authors: Fan DaiAbstract:Aim To study the Tumor specific cytotoxicity induced by Ehrlich Ascites Tumor cell vaccine. Methods Using paraformaldehyde treated Ehrlich Ascites Tumor cells as the vaccine, an animal model of Tumor vaccination was established. The Tumor nodules of the immunized Balb/c mice after subcutaneous Tumor rechallenge were observed by HE staining. Splenocytes were separated from immunized, Tumor bearing and control mice, respectively. The 51Cr release assay was used to examine the cytotoxicities of three kinds of the splenocytes to Ehrlich Ascites Tumor cells and Sp2/0 cells. Results It was showed that the Tumor cells in Tumor nodules of immunized mice exhibited almost complete necrosis and simultaneously possessed a large number of lymphocytes infiltration, fibroblasts and small vessels proliferation, while no phenomena mentioned above was found in the control mice. Specific cytotoxic rate of splenocytes from the immunized mice to the Ehrlich Ascites Tumor was (42.3±3.2)%,significantly higher than that from the other two groups(P∨0.05) and also significantly higher than that to Sp2/0 cells (8.8±0.4)%(P∨0.05). Conclusion Ehrlich Ascites Tumor cell vaccine can induce significantly Tumor specific cytotoxicity in vivo.
Zheng Ma - One of the best experts on this subject based on the ideXlab platform.
-
Immuno-protective effect of Tumor cell vaccine on Kunming mice bearing Ehrlich Ascites Tumor.
World Journal of Gastroenterology, 1998Co-Authors: Zheng Ma, Shao-juan Zhou, Kaichun Wu, Taidong Qiao, Bao-jun ChenAbstract:AIM: To evaluate the immunity of chemically modified Tumor cell vaccine. METHODS: Tumor cell vaccines (TCV) were prepared by incubating the live Ehrlich Ascites Tumor cells with concanavalin A-mitomycin C (ConA-MMC), mitomycin C (MMC), concanavalin A-glutaraldehyde (ConA-Glu), glutaraldehyde ( Glu ), or paraformaldehyde ( Para ), respectively. The whole cell or soluble forms of the vaccines were administered intraperitoneally into Kunming mice once a week for three times prior to the intraperitoneal inoculation of a lethal dose of live Tumor cells. A second challenge with live Tumor cells was given four weeks later. Survival and antibody production of the mice were analyzed. RESULTS: After the first challenge, the mice, received whole TCV of ConA-MMC, MMC (P < 0.01) and Glu (P < 0.05) promoted survival incidence than the controls. All the treated mice had the survival time prolonged. ConA-MMC vaccine treated mice had longer survival days than that of ConA-Glu ones (P < 0.05). For the soluble TCV immunized mice, those treated with vaccines of Para (P < 0.01), ConA-Para and ConA-Glu (P < 0.05) had longer survival periods compared with that of the controls. Following the second challenge, survival incidence of the mice received vaccines of ConA-MMC, MMC, ConA-Glu or Glu was significantly increased (P < 0.01). Moreover, all the treated mice had the survival time prolonged, and ConA-MMC vaccine treated mice had longer survival days than that of Para treated ones (P < 0.05). Antibodies against Ehrlich Ascites Tumor cells were found to be positive in sera of the mice treated with whole TCV of ConA-MMC. CONCLUSION: Ehrlich Ascites Tumor cells are immunogenic when treated with ConA-MMC, MMC, ConA-Glu, Glu or Para, which might act as safe and effective Tumor vaccines with safety and effectiveness.
Lech Wojtczak - One of the best experts on this subject based on the ideXlab platform.
-
pantothenic acid and its derivatives protect Ehrlich Ascites Tumor cells against lipid peroxidation
Free Radical Biology and Medicine, 1995Co-Authors: Vyacheslav S Slyshenkov, Mariola Rakowska, Andrei G Moiseenok, Lech WojtczakAbstract:Abstract Preincubation of Ehrlich Ascites Tumor cells at 22 or 32°C, but not at 0°C, with pantothenic acid, 4′-phosphopantothenic acid, pantothenol, or pantethine reduced lipid peroxidation (measured by production of thiobarbituric acid-reactive compounds) induced by the Fenton reaction (Fe 2+ + H 2 O 2 ) and partly protected the plasma membrane against the leakiness to cytoplasmic proteins produced by the same reagent. Pantothenic acid and its derivatives did not inhibit (Fe 2+ + H 2 O 2 )-induced peroxidation of phospholipid multilamellar vesicles, thus indicating that their effect on the cells was not due to the scavenging mechanism. Homopantothenic acid and its 4′-phosphate ester (which are not precursors of CoA) neither protected Ehrlich Ascites Tumor cells against lipid peroxidation nor prevented plasma membrane leakiness under the same conditions. Incubation of the cells with pantothenic acid, 4′-phosphopantothenic acid, pantothenol, or pantethine significantly increased the amount of cellular CoA and potentiated incorporation of added palmitate into phospholipids and cholesterol esters. It is concluded that pantothenic acid and its related compounds protect the plasma membrane of Ehrlich Ascites Tumor cells against the damage by oxygen free radicals due to increasing cellular level of CoA. The latter compound may act by diminishing propagation of lipid peroxidation and promoting repair mechanisms, mainly the synthesis of phospholipids.
Lidia Wlodek - One of the best experts on this subject based on the ideXlab platform.
-
selective effects of diallyl disulfide a sulfane sulfur precursor in the liver and Ehrlich Ascites Tumor cells
European Journal of Pharmacology, 2007Co-Authors: Malgorzata Iciek, Joanna Marcinek, Urszula Mleczko, Lidia WlodekAbstract:The present in vivo studies demonstrated that diallyl disulfide (DADS), occurring in garlic, elevated hepatic sulfane sulfur level and activities of γ-cystathionase and 3-mercaptopyruvate sulfotransferase in healthy mice but did not affect the hepatic glutathione level. DADS efficiently corrected the concentrations of glutathione and sulfane sulfur, and ameliorated γ-cystathionase activity that had been lowered in the livers of Ehrlich Ascites Tumor-bearing mice. In Ehrlich Ascites Tumor cells, diallyl disulfide did not alter bound sulfane sulfur level, sulfotransferases activity or glutathione level. These data indicate that this compound is capable of acting efficiently and selectively only in the liver and can be used for hepatoprotection during chemotherapy.