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Everaldo Attard - One of the best experts on this subject based on the ideXlab platform.
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The Phytochemical Constitution of Maltese Medicinal Plants – Propagation, Isolation and Pharmacological Testing
Phytochemicals - Isolation Characterisation and Role in Human Health, 2015Co-Authors: Everaldo Attard, Henrietta Attard, Antoine Tanti, Mario Sciberras Jurgen Azzopardi, Victor Pace, Neville Buttigieg, Andrew Mangionrandon, Bernardette Rossi, Marie Josette Parnis, Michelle Zammit Karin VellaAbstract:Fourteen Maltese medicinal plants, that were significantly used in tradition, were studied for their phytochemical constitution and pharmacological activities. These include Drimia maritima (L.) Stearn, Ecballium Elaterium (L.) A.Rich., Mentha pulegium L., Salvia officinalis L., Verbena officinalis L., Hedera helix L., Crataegus monogyna Jacq., Calendula officinalis L., Melissa officinalis L., Olea europea L., Urtica dubia Forsk., Capparis spinosa L., Ephedra fragilis Desf. and Nicotiana glauca RC Graham. Cultivation and micropropagation practices, in relation to secondary metabolite production, were also considered for some these plants. This article highlights the main findings for these plants.peer-reviewe
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Use of Extracts from Squirting Cucumber ( Ecballium Elaterium ) Seeds in Health
Nuts and Seeds in Health and Disease Prevention, 2011Co-Authors: Everaldo AttardAbstract:Publisher Summary This chapter summarizes the nutritional benefits of squirting cucumber extracts. The fruit is considered to be a source of pharmacologically active metabolites. Important metabolites include carbohydrates, gum, leukoanthocyanins, tannins, triterpenoids, and peptides. The plant biosynthesizes and stores specific polypeptides, termed protease inhibitors, in the seed extracts. Seed extracts obtained from Ecballium Elaterium contain inhibitors for at least four different serine proteinases—trypsin inhibitors I, II, and III, also known as the trypsin isoinhibitors, chymotrypsin inhibitor, subtilisin inhibitor, and elastase inhibitor, and astacus protease inhibitor. Ecballium Elaterium trypsin isoinhibitor II (EETI II) is a short peptide, which is easily synthesized and mutated, can be modified to become a selective inhibitor against other proteases—for example, modification of EETI II may lead to selective inhibition of porcine pancreatic elastase. From ethnobotanical and scientific records, adverse effects and reactions have been mostly related to medicinal sources within the plant other than the seeds. These toxic effects are primarily caused by the juice surrounding the seeds.
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Antitrypsin activity of extracts from Ecballium Elaterium seeds.
Fitoterapia, 2008Co-Authors: Everaldo Attard, Henrietta AttardAbstract:The antitrypsin activity of extracts obtained from two different seed types of Ecballium Elaterium, was tested in vitro. The presence of trypsin inhibitors in three chromatographed samples for each extract, showed a strong and specific antitrypsin activity.
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Immunomodulatory activity of cucurbitacin E isolated from Ecballium Elaterium
Fitoterapia, 2005Co-Authors: Everaldo Attard, Mark Brincat, Alfred CuschieriAbstract:The immunomodulatory effect of cucurbitacin E, extracted from Ecballium Elaterium, was tested on peripheral human lymphocytes. These lymphocytes were co-cultured with cancer cells and an interesting lymphocyte-mediated cytotoxicity was observed.
Dibos Chloé - One of the best experts on this subject based on the ideXlab platform.
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Plant – pollinator interactions : characterisation of pollen quality during its ontogenesis, its presentation and its transport on honey bee body in two cucurbitaceae
2010Co-Authors: Dibos ChloéAbstract:Chez de nombreuses Angiospermes, la pollinisation croisée est nécessaire pour le succès de la reproduction. La plupart de ces plantes ont évolué afin de favoriser la pollinisation entomophile, principalement assurée par les abeilles. C’est le cas des Cucurbitacées, dont le melon (Cucumis melo L.), importante culture du sud de la France et le concombre d’âne (Ecballium Elaterium (L.) A. Rich.) plante spontanée endémique du bassin méditerranéen, les deux modèles de cette étude. Afin de mieux comprendre les relations plante-pollinisateur,nous avons choisi de caractériser le couple pollen de Cucurbitacées/abeille domestique (Apis mellifera L.) à travers la production de pollen, sa présentation sur la fleur et son transport sur l’abeille. Nous avons montré que, chez ces plantes, l’ontogenèse du pollen s’accompagne d’erreurs développementales conduisant à la formation de près de 3% de grains de pollen vides. De plus, nous avons mis en évidence que chez E. Elaterium, des erreurs méiotiques entraînaient la formation de grains de pollen génétiquement anormaux qui pourraient être source d’autopolyploïdie. Chez C. melo, nos résultats ont révélé que la mise en contact du pollen avec le milieu environnant entraîne une baisse de 30% de sa viabilité, mais que celle-ci reste stable jusqu’à la fin de l’anthèse. L’aptitude à germer, quant à elle, finit d’être acquise juste avant anthèse et décroît de 12% en fin de période d’anthèse. Nous avons montré que le pollen de C. melo transporté sur le corps de l’abeille pouvait soit avoir une viabilité et une aptitude à germer préservée, probablement par protection contre la déshydratation, soit perdre quasi-totalement sa viabilité et son aptitude à germer. Enfin, nous avons détecté des composés biochimiques spécifiques des abeilles protégeant ou diminuant la qualité reproductrice du pollenFor many flowering plant species cross pollination is necessary to ensure reproductive success. Most of these plants have evolved to encourage insect pollination which is mainly carried out by bees. Such is the case of plants in the Cucurbitaceae family, including the cantaloupe (Cucumis melo L.), an important crop of the South of France, and squirting cucumber (Ecballium Elaterium (L.) A. Rich.), a wild plant restricted to the Mediterranean Basin, the two vegetal models used in this study. To better understand plant-pollinator relations, we proposed to characterise the interaction between Cucurbitaceae pollen and the honey bee (Apis mellifera L.) through pollen production, its presentation and its transport on the honey bee body. Our results showed that developmental anomalies took place during pollen ontogenesis in these species leading to 3% of the pollen grains produced to be empty. Moreover, we demonstrated that meiotic abnormalities in E. Elaterium leading to the production of genetically abnormal pollen grains could be a source of autopolyploidy. At anthesis in C. melo, our results showed that pollen viability decreased to 30% when pollen was exposed to environmental conditions, then remained stable during the period of anthesis.The ability for pollen to germinate was completed just before anthesis then decreased to 12% at the end of anthesis. We determined that viability and germinability of cantaloupe pollen carried on the honey bee body can be enhanced or decreased according to which specific honey bee biochemical compounds were detecte
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Interactions plante - pollinisateur : caractérisation de la qualité du pollen de deux cucurbitacées durant son ontogenèse, sa présentation et son transport sur le corps de l'abeille domestique
HAL CCSD, 2010Co-Authors: Dibos ChloéAbstract:For many flowering plant species cross pollination is necessary to ensure reproductive success. Most of these plants have evolved to encourage insect pollination which is mainly carried out by bees. Such is the case of plants in the Cucurbitaceae family, including the cantaloupe (Cucumis melo L.), an important crop of the South of France, and squirting cucumber (Ecballium Elaterium (L.) A. Rich.), a wild plant restricted to the Mediterranean Basin, the two vegetal models used in this study. To better understand plant-pollinator relations, we proposed to characterise the interaction between Cucurbitaceae pollen and the honey bee (Apis mellifera L.) through pollen production, its presentation and its transport on the honey bee body. Our results showed that developmental anomalies took place during pollen ontogenesis in these species leading to 3% of the pollen grains produced to be empty. Moreover, we demonstrated that meiotic abnormalities in E. Elaterium leading to the production of genetically abnormal pollen grains could be a source of autopolyploidy. At anthesis in C. melo, our results showed that pollen viability decreased to 30% when pollen was exposed to environmental conditions, then remained stable during the period of anthesis.The ability for pollen to germinate was completed just before anthesis then decreased to 12% at the end of anthesis. We determined that viability and germinability of cantaloupe pollen carried on the honey bee body can be enhanced or decreased according to which specific honey bee biochemical compounds were detectedChez de nombreuses Angiospermes, la pollinisation croisée est nécessaire pour le succès de la reproduction. La plupart de ces plantes ont évolué afin de favoriser la pollinisation entomophile, principalement assurée par les abeilles. C’est le cas des Cucurbitacées, dont le melon (Cucumis melo L.), importante culture du sud de la France et le concombre d’âne (Ecballium Elaterium (L.) A. Rich.) plante spontanée endémique du bassin méditerranéen, les deux modèles de cette étude. Afin de mieux comprendre les relations plante-pollinisateur,nous avons choisi de caractériser le couple pollen de Cucurbitacées/abeille domestique (Apis mellifera L.) à travers la production de pollen, sa présentation sur la fleur et son transport sur l’abeille. Nous avons montré que, chez ces plantes, l’ontogenèse du pollen s’accompagne d’erreurs développementales conduisant à la formation de près de 3% de grains de pollen vides. De plus, nous avons mis en évidence que chez E. Elaterium, des erreurs méiotiques entraînaient la formation de grains de pollen génétiquement anormaux qui pourraient être source d’autopolyploïdie. Chez C. melo, nos résultats ont révélé que la mise en contact du pollen avec le milieu environnant entraîne une baisse de 30% de sa viabilité, mais que celle-ci reste stable jusqu’à la fin de l’anthèse. L’aptitude à germer, quant à elle, finit d’être acquise juste avant anthèse et décroît de 12% en fin de période d’anthèse. Nous avons montré que le pollen de C. melo transporté sur le corps de l’abeille pouvait soit avoir une viabilité et une aptitude à germer préservée, probablement par protection contre la déshydratation, soit perdre quasi-totalement sa viabilité et son aptitude à germer. Enfin, nous avons détecté des composés biochimiques spécifiques des abeilles protégeant ou diminuant la qualité reproductrice du polle
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Interactions plante - pollinisateur (caractérisation de la qualité du pollen de deux cucurbitacées durant son ontogenèse, sa présentation et son transport sur le corps de l'abeille domestique)
2010Co-Authors: Dibos Chloé, El Maataoui Mohamed, Suchail SéverineAbstract:Chez de nombreuses Angiospermes, la pollinisation croisée est nécessaire pour le succès de la reproduction. La plupart de ces plantes ont évolué afin de favoriser la pollinisation entomophile, principalement assurée par les abeilles. C est le cas des Cucurbitacées, dont le melon (Cucumis melo L.), importante culture du sud de la France et le concombre d âne (Ecballium Elaterium (L.) A. Rich.) plante spontanée endémique du bassin méditerrane en, les deux modèles de cette étude. Afin de mieux comprendre les relations plante-pollinisateur,nous avons choisi de caractériser le couple pollen de Cucurbitacées/abeille domestique (Apis mellifera L.) à travers la production de pollen, sa présentation sur la fleur et son transport sur l abeille. Nous avons montré que, chez ces plantes, l ontogenèse du pollen s accompagne d erreurs développementales conduisant à la formation de près de 3% de grains de pollen vides. De plus, nous avons mis en évidence que chez E. Elaterium, des erreurs méiotiques entraînaient la formation de grains de pollen génétiquement anormaux qui pourraient être source d autopolyploïdie. Chez C. melo, nos résultats ont révélé que la mise en contact du pollen avec le milieu environnant entraîne une baisse de 30% de sa viabilité, mais que celle-ci reste stable jusqu à la fin de l anthèse. L aptitude à germer, quant à elle, finit d être acquise juste avant anthèse et décroît de 12% en fin de période d anthèse. Nous avons montré que le pollen de C. melo transporté sur le corps de l abeille pouvait soit avoir une viabilité et une aptitude à germer préservée, probablement par protection contre la déshydratation, soit perdre quasi-totalement sa viabilité et son aptitude à germer. Enfin, nous avons détecté des composés biochimiques spécifiques des abeilles protégeant ou diminuant la qualité reproductrice du pollenFor many flowering plant species cross pollination is necessary to ensure reproductive success. Most of these plants have evolved to encourage insect pollination which is mainly carried out by bees. Such is the case of plants in the Cucurbitaceae family, including the cantaloupe (Cucumis melo L.), an important crop of the South of France, and squirting cucumber (Ecballium Elaterium (L.) A. Rich.), a wild plant restricted to the Mediterranean Basin, the two vegetal models used in this study. To better understand plant-pollinator relations, we proposed to characterise the interaction between Cucurbitaceae pollen and the honey bee (Apis mellifera L.) through pollen production, its presentation and its transport on the honey bee body. Our results showed that developmental anomalies took place during pollen ontogenesis in these species leading to 3% of the pollen grains produced to be empty. Moreover, we demonstrated that meiotic abnormalities in E. Elaterium leading to the production of genetically abnormal pollen grains could be a source of autopolyploidy. At anthesis in C. melo, our results showed that pollen viability decreased to 30% when pollen was exposed to environmental conditions, then remained stable during the period of anthesis.The ability for pollen to germinate was completed just before anthesis then decreased to 12% at the end of anthesis. We determined that viability and germinability of cantaloupe pollen carried on the honey bee body can be enhanced or decreased according to which specific honey bee biochemical compounds were detectedAVIGNON-Bib. numérique (840079901) / SudocSudocFranceF
Belgacem Hanchi - One of the best experts on this subject based on the ideXlab platform.
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Targetting alphavbeta3 and alpha5beta1 integrins with Ecballium Elaterium (L.) A. Rich. seed oil
Biomedicine and Pharmacotherapy, 2016Co-Authors: Imen Touihri-barakati, Olfa Kallech-ziri, Abdennacer Boulila, Naziha Marrakchi, Belgacem Hanchi, Khaoula Khwaldia, Karim Hosni, José LuisAbstract:In the present study, the effect of Ecbalium Elaterium seed oil on adhesion, migration and proliferation of human brain cancer cell line (U87) was determined. Treatment of U87 cell line with the seed oil resulted in strong inhibition of their adhesion to fibrinogen (Fg), fibronectin (Fn). It also reduced their migration and proliferation in a dose-dependent manner without being cytotoxic. Concomitantly, by using Matrigel TM assays, the oil significantly inhibited angiogenesis. The anti-tumor effect of the oil is specifically mediated by avb3 and a5b1 integrins. The presence of integrin antagonists in seed oil from E. Elaterium could be used for the development of anticancer drugs with targeted " multi-modal " therapies combining anti-adhesif, antiproliferative, antimetastasic and anti-angiogenic, approaches.
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Targetting αvβ3 and α5β1 integrins with Ecballium Elaterium (L.) A. Rich. seed oil
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2016Co-Authors: Imen Touihri-barakati, Olfa Kallech-ziri, Abdennacer Boulila, Naziha Marrakchi, Belgacem Hanchi, Khaoula Khwaldia, Karim Hosni, José LuisAbstract:In the present study, the effect of Ecbalium Elaterium seed oil on adhesion, migration and proliferation of human brain cancer cell line (U87) was determined. Treatment of U87 cell line with the seed oil resulted in strong inhibition of their adhesion to fibrinogen (Fg), fibronectin (Fn). It also reduced their migration and proliferation in a dose-dependent manner without being cytotoxic. Concomitantly, by using Matrigel™ assays, the oil significantly inhibited angiogenesis. The anti- tumor effect of the oil is specifically mediated by αvβ3 and α5β1 integrins. The presence of integrin antagonists in seed oil from E. Elaterium could be used for the development of anticancer drugs with targeted "multi-modal" therapies combining anti-adhesif, antiproliferative, antimetastasic and anti-angiogenic, approaches.
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Ecballium Elaterium (L.) A. Rich. seed oil: Chemical composition and antiproliferative effect on human colonic adenocarcinoma and fibrosarcoma cancer cell lines
Arabian Journal of Chemistry, 2015Co-Authors: Imen Touihri, Olfa Kallech-ziri, Abdennacer Boulila, Saloua Fatnassi, Naziha Marrakchi, José Luis, Belgacem HanchiAbstract:Abstract In this work the physicochemical characteristics including fatty acids, tocopherols and sterols composition of Ecballium Elaterium (L.) A. Rich seed oil was determined. Results showed that linoleic acid (48.64%) and punicic acid (22.38%) were the major polyunsaturated fatty acids. Among the phytosterols, β-sitosterol was the most abundant (396.25 mg/100 g), while the major tocopherol form was γ-tocopherol (44.23 mg/100 g). In addition, we evaluated for the first time the effect of E. Elaterium seed oil on the growth of human colonic adenocarcinoma (HT29) and fibrosarcoma (HT1080) cell lines. The original finding was its potent antiproliferative effect on both tumour cell lines. This effect was dose-dependent, with half-maximal inhibition values of IC 50 = 4.86 μg/ml and 4.16 μg/ml respectively. This pilot study opens the way for further investigation about the potential use of E. Elaterium as an anticancer agent.
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Ecballium Elaterium (L.) A. Rich. seed oil: Chemical composition and antiproliferative effect on human colonic adenocarcinoma and fibrosarcoma cancer cell lines
Arabian Journal of Chemistry, 2015Co-Authors: Imen Touihri, Olfa Kallech-ziri, Abdennacer Boulila, Saloua Fatnassi, Naziha Marrakchi, José Luis, Belgacem HanchiAbstract:In this work the physicochemical characteristics including fatty acids, tocopherols and sterols composition of Ecballium Elaterium (L.) A. Rich seed oil was determined. Results showed that linoleic acid (48.64%) and punicic acid (22.38%) were the major polyunsaturated fatty acids. Among the phytosterols, b-sitosterol was the most abundant (396.25 mg/100 g), while the major tocopherol form was c-tocopherol (44.23 mg/100 g). In addition, we evaluated for the first time the effect of E. Elaterium seed oil on the growth of human colonic adenocarcinoma (HT29) and fibrosarcoma (HT1080) cell lines. The original finding was its potent antiproliferative effect on both tumour cell lines. This effect was dose-dependent, with half-maximal inhibition values of IC50 = 4.86 lg/ml and 4.16 lg/ml respectively. This pilot study opens the way for further investigation about the potential use of E. Elaterium as an anticancer agent.
José Luis - One of the best experts on this subject based on the ideXlab platform.
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Targetting alphavbeta3 and alpha5beta1 integrins with Ecballium Elaterium (L.) A. Rich. seed oil
Biomedicine and Pharmacotherapy, 2016Co-Authors: Imen Touihri-barakati, Olfa Kallech-ziri, Abdennacer Boulila, Naziha Marrakchi, Belgacem Hanchi, Khaoula Khwaldia, Karim Hosni, José LuisAbstract:In the present study, the effect of Ecbalium Elaterium seed oil on adhesion, migration and proliferation of human brain cancer cell line (U87) was determined. Treatment of U87 cell line with the seed oil resulted in strong inhibition of their adhesion to fibrinogen (Fg), fibronectin (Fn). It also reduced their migration and proliferation in a dose-dependent manner without being cytotoxic. Concomitantly, by using Matrigel TM assays, the oil significantly inhibited angiogenesis. The anti-tumor effect of the oil is specifically mediated by avb3 and a5b1 integrins. The presence of integrin antagonists in seed oil from E. Elaterium could be used for the development of anticancer drugs with targeted " multi-modal " therapies combining anti-adhesif, antiproliferative, antimetastasic and anti-angiogenic, approaches.
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Targetting αvβ3 and α5β1 integrins with Ecballium Elaterium (L.) A. Rich. seed oil
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2016Co-Authors: Imen Touihri-barakati, Olfa Kallech-ziri, Abdennacer Boulila, Naziha Marrakchi, Belgacem Hanchi, Khaoula Khwaldia, Karim Hosni, José LuisAbstract:In the present study, the effect of Ecbalium Elaterium seed oil on adhesion, migration and proliferation of human brain cancer cell line (U87) was determined. Treatment of U87 cell line with the seed oil resulted in strong inhibition of their adhesion to fibrinogen (Fg), fibronectin (Fn). It also reduced their migration and proliferation in a dose-dependent manner without being cytotoxic. Concomitantly, by using Matrigel™ assays, the oil significantly inhibited angiogenesis. The anti- tumor effect of the oil is specifically mediated by αvβ3 and α5β1 integrins. The presence of integrin antagonists in seed oil from E. Elaterium could be used for the development of anticancer drugs with targeted "multi-modal" therapies combining anti-adhesif, antiproliferative, antimetastasic and anti-angiogenic, approaches.
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Ecballium Elaterium (L.) A. Rich. seed oil: Chemical composition and antiproliferative effect on human colonic adenocarcinoma and fibrosarcoma cancer cell lines
Arabian Journal of Chemistry, 2015Co-Authors: Imen Touihri, Olfa Kallech-ziri, Abdennacer Boulila, Saloua Fatnassi, Naziha Marrakchi, José Luis, Belgacem HanchiAbstract:Abstract In this work the physicochemical characteristics including fatty acids, tocopherols and sterols composition of Ecballium Elaterium (L.) A. Rich seed oil was determined. Results showed that linoleic acid (48.64%) and punicic acid (22.38%) were the major polyunsaturated fatty acids. Among the phytosterols, β-sitosterol was the most abundant (396.25 mg/100 g), while the major tocopherol form was γ-tocopherol (44.23 mg/100 g). In addition, we evaluated for the first time the effect of E. Elaterium seed oil on the growth of human colonic adenocarcinoma (HT29) and fibrosarcoma (HT1080) cell lines. The original finding was its potent antiproliferative effect on both tumour cell lines. This effect was dose-dependent, with half-maximal inhibition values of IC 50 = 4.86 μg/ml and 4.16 μg/ml respectively. This pilot study opens the way for further investigation about the potential use of E. Elaterium as an anticancer agent.
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Ecballium Elaterium (L.) A. Rich. seed oil: Chemical composition and antiproliferative effect on human colonic adenocarcinoma and fibrosarcoma cancer cell lines
Arabian Journal of Chemistry, 2015Co-Authors: Imen Touihri, Olfa Kallech-ziri, Abdennacer Boulila, Saloua Fatnassi, Naziha Marrakchi, José Luis, Belgacem HanchiAbstract:In this work the physicochemical characteristics including fatty acids, tocopherols and sterols composition of Ecballium Elaterium (L.) A. Rich seed oil was determined. Results showed that linoleic acid (48.64%) and punicic acid (22.38%) were the major polyunsaturated fatty acids. Among the phytosterols, b-sitosterol was the most abundant (396.25 mg/100 g), while the major tocopherol form was c-tocopherol (44.23 mg/100 g). In addition, we evaluated for the first time the effect of E. Elaterium seed oil on the growth of human colonic adenocarcinoma (HT29) and fibrosarcoma (HT1080) cell lines. The original finding was its potent antiproliferative effect on both tumour cell lines. This effect was dose-dependent, with half-maximal inhibition values of IC50 = 4.86 lg/ml and 4.16 lg/ml respectively. This pilot study opens the way for further investigation about the potential use of E. Elaterium as an anticancer agent.
Adel Kadri - One of the best experts on this subject based on the ideXlab platform.
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Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium Elaterium methanol fruits extract
Food Science and Technology, 2017Co-Authors: Samir Felhi, Mongi Saoudi, Amal Daoud, Hafedh Hajlaoui, Marwa Ncir, Rim Chaabane, Abdelfattah El Feki, Néji Gharsallah, Adel KadriAbstract:Abstract Ecballium Elaterium species are mostly used as therapeutic agents and food ingredient. The current work was designed to investigate phytochemical contents, antioxidant, antibacterial, and anti-inflammatory properties of methanol fruits extract of Ecballium Elaterium . Good antioxidant activity was observed with IC 50 values of 156 ± 4 and 377 ± 6 μg/mL for DPPH and ABTS, respectively, and EC 50 of 126 ± 4 µg/mL for FRAP assays, which is related with their richness in total phenolic, flavonoid and condensed tannins contents. The results of antibacterial activity showed the effectiveness of methanol extract against Bacillus cereus with value of inhibition zone diameter of 15 ± 0 mm and a MIC and MBC values of 6 ± 0 and 12 ± 0 mg/mL, respectively. The in vivo anti-inflammatory effects have been also studied by carrageenan induced rat paw edema assay and the results revealed that a dose of 75 mg/kg induced a significant inhibition of 66.4% at 2 h. FT-IR spectral data justified the presence of biological functional groups such as ─OH, C ─H, C ─O, C ─C and C=O. These results highlighted the potential using of
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Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium Elaterium seeds and peels fruits
Food Science and Technology, 2017Co-Authors: Samir Felhi, Amal Daoud, Hafedh Hajlaoui, Néji Gharsallah, Kais Mnafgui, Adel KadriAbstract:Abstract Ecballium Elaterium is a perennial herb with multiple medicinal properties. It was widely used in folk medicine as cathartic, anti-inflammatory and analgesic agents. The present study was devoted to investigate the effect of diethyl ether, acetone, and methanol solvent on the extraction, phytochemicals profiles, antioxidant and antimicrobial activities of Ecballium Elaterium seeds and peels fruits. The total phenolic, flavonoid, flavonol, condensed tannins and carotenoids contents were estimated. Maximum phenolic (107 ± 4 mg GAE/g) and flavonoid (18 ± 0 mg QE/g) contents were also found in the methanol peels fruits extract. Results showed that methanol peels fruits extract have the highest antioxidant activity with IC 50 values of 1.2 ± 0.1 and 1 ± 0 mg/mL for DPPH and ABTS, respectively, and EC 50 value of 1040 ± 5 mg/mL for reducing power assays. Acetone and diethyl ether peels fruits extracts showed the best antibacterial agents especially against Micrococcus luteus , however no antifungal activity was observed. Spectral data of FT-IR analysis of
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Investigation of phytochemical contents, in vitro antioxidant and antibacterial behavior and in vivo anti-inflammatory potential of Ecballium Elaterium methanol fruits extract
2017Co-Authors: Samir Felhi, Mongi Saoudi, Amal Daoud, Hafedh Hajlaoui, Marwa Ncir, Rim Chaabane, Abdelfattah El Feki, Néji Gharsallah, Adel KadriAbstract:Abstract Ecballium Elaterium species are mostly used as therapeutic agents and food ingredient. The current work was designed to investigate phytochemical contents, antioxidant, antibacterial, and anti-inflammatory properties of methanol fruits extract of Ecballium Elaterium. Good antioxidant activity was observed with IC50 values of 156 ± 4 and 377 ± 6 μg/mL for DPPH and ABTS, respectively, and EC50 of 126 ± 4 µg/mL for FRAP assays, which is related with their richness in total phenolic, flavonoid and condensed tannins contents. The results of antibacterial activity showed the effectiveness of methanol extract against Bacillus cereus with value of inhibition zone diameter of 15 ± 0 mm and a MIC and MBC values of 6 ± 0 and 12 ± 0 mg/mL, respectively. The in vivo anti-inflammatory effects have been also studied by carrageenan induced rat paw edema assay and the results revealed that a dose of 75 mg/kg induced a significant inhibition of 66.4% at 2 h. FT-IR spectral data justified the presence of biological functional groups such as ─OH, C─H, C─O, C─C and C=O. These results highlighted the potential using of Ecballium Elaterium fruits extract as natural antimicrobial, antioxidant and anti-inflammatory agents for food applications and for the pharmaceutical industry.