The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform

Semra Şardaş - One of the best experts on this subject based on the ideXlab platform.

  • monitoring of buccal epithelial cells by alkaline comet assay single cell gel Electrophoresis Technique in cytogenetic evaluation of chlorhexidine
    Clinical Oral Investigations, 2002
    Co-Authors: Kaya Eren, Nurdan Ozmeric, Semra Şardaş
    Abstract:

    During the last few years, there has been increasing interest in buccal epithelial cells for cytogenetic evaluation of different materials. In the present study, the use of these cells and peripheral lymphocytes for cytogenetic evaluation of chlorhexidine digluconate (CHX) with comet assay (single cell gel Electrophoresis, or SCGE) is reported. This Technique detects DNA strand breaks in individual cells in alkaline conditions. Thirteen volunteers were requested to rinse their mouths with 0.12% CHX solution for 18 days. Buccal epithelial cells and peripheral blood lymphocytes were obtained from all participants at baseline and the end of the experimental period. One hundred cells per subject were analysed for the DNA damage. A statistical increase was observed in the damaged buccal and blood cells after the CHX application. The mean grade of damage in buccal cells was statistically different from that in blood cells. Due to minimal absorption of chlorhexidine into the tissues and low concentrations of free chlorhexidine in the oral cavity, the DNA damage produced by chlorhexidine in lymphocytes was lower than in buccal epithelial cells. As chlorhexidine does not accumulate in the body, the frequencies of DNA damage could be transient. Detected DNA damage after CHX use might be the indication of an earlier effect, before DNA repair begins, and could be reversible.

  • Use of alkaline Comet assay (single cell gel Electrophoresis Technique) to detect DNA damages in lymphocytes of operating room personnel occupationally exposed to anaesthetic gases
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 1998
    Co-Authors: Semra Şardaş, N. Aygün, M. Gamli, Y. Ünal, N Ünal, N Berk, Ali Esat Karakaya
    Abstract:

    Here, we report the possible in vivo induction DNA damage by exposure to various waste anaesthetic gases such as halothane, nitrous oxide and isoflurane. The alkaline comet assay (single cell gel Electrophoresis Technique) was carried out on 66 operating room personnel (anaesthetists [doctors]; anaesthesia nurses and anaesthesia unit technicians) currently employed at the Ankara Hospital in Turkey. A significant increase in the number of lymphocytes with DNA migration was observed in operating room personnel as compared to controls. Also, the extent of damage in exposed smokers were significantly higher than exposed nonsmokers. This study supports the existence of an association between DNA damage and occupational exposure to inhalation anaesthetics.

Adela Rosa Rodríguez - One of the best experts on this subject based on the ideXlab platform.

  • 2 dimensional gel Electrophoresis Technique for yeast selenium containing proteins sample preparation and ms approaches for processing 2 d gel protein spots
    Journal of Analytical Atomic Spectrometry, 2004
    Co-Authors: Hubert Chassaigne, Cyrille C. Chéry, Guy Bordin, Frank Vanhaecke, Adela Rosa Rodríguez
    Abstract:

    A novel integrated approach is proposed for the analysis of intact yeast selenium-containing proteins purified by a gel Electrophoresis Technique. The strategy consists of three components: high-resolution two-dimensional gel Electrophoresis (2-DE) for proteins, laser ablation-inductively coupled plasma-dynamic reaction cell-mass spectrometry (LA-ICP-DRC-MS) for selenium detection and protein characterisation by electrospray mass spectrometry (ion-trap (IT MS) and time-of-flight (TOF MS) instruments). High-resolution 2-DE is the method of choice for protein purification before their characterisation by mass spectrometry Techniques. SDS-PAGE which is a denaturating Technique is applicable in this case as the selenium is covalently bound to the proteins. LA-ICP-DRC-MS was used, as optimised in previous work for several elements, which also showed that the limit of detection (absolute LOD of 0.5 pg Se per crater or 70 ng Se g−1 gel) was low enough to detect Se in the protein spots. Protein spots were excised from 2-D gels, destained and extracted. Information on the mass of Se-containing proteins were obtained from IT MS and TOF MS. Measurements of the proteins were made with two different MS instruments in order to validate the results and obtain the highest accuracy and resolution on the molecular masses. Up to ten selenium-containing proteins were characterised in terms of molecular mass in the range 9–20 kDa. This strategy has particular application to the possible establishment of a 2-D reference map (Se content and molecular masses) for Se-containing proteins in yeast.

  • 2-Dimensional gel Electrophoresis Technique for yeast selenium-containing proteins—sample preparation and MS approaches for processing 2-D gel protein spots
    J. Anal. At. Spectrom., 2004
    Co-Authors: Hubert Chassaigne, Cyrille C. Chéry, Guy Bordin, Frank Vanhaecke, Adela Rosa Rodríguez
    Abstract:

    A novel integrated approach is proposed for the analysis of intact yeast selenium-containing proteins purified by a gel Electrophoresis Technique. The strategy consists of three components: high-resolution two-dimensional gel Electrophoresis (2-DE) for proteins, laser ablation-inductively coupled plasma-dynamic reaction cell-mass spectrometry (LA-ICP-DRC-MS) for selenium detection and protein characterisation by electrospray mass spectrometry (ion-trap (IT MS) and time-of-flight (TOF MS) instruments). High-resolution 2-DE is the method of choice for protein purification before their characterisation by mass spectrometry Techniques. SDS-PAGE which is a denaturating Technique is applicable in this case as the selenium is covalently bound to the proteins. LA-ICP-DRC-MS was used, as optimised in previous work for several elements, which also showed that the limit of detection (absolute LOD of 0.5 pg Se per crater or 70 ng Se g−1 gel) was low enough to detect Se in the protein spots. Protein spots were excised from 2-D gels, destained and extracted. Information on the mass of Se-containing proteins were obtained from IT MS and TOF MS. Measurements of the proteins were made with two different MS instruments in order to validate the results and obtain the highest accuracy and resolution on the molecular masses. Up to ten selenium-containing proteins were characterised in terms of molecular mass in the range 9–20 kDa. This strategy has particular application to the possible establishment of a 2-D reference map (Se content and molecular masses) for Se-containing proteins in yeast.

Maria Rosaria Scarfì - One of the best experts on this subject based on the ideXlab platform.

  • DNA damage by carbon nanotubes using the single cell gel Electrophoresis Technique.
    Methods in molecular biology (Clifton N.J.), 2010
    Co-Authors: Olga Zeni, Maria Rosaria Scarfì
    Abstract:

    The single-cell gel Electrophoresis (SCGE) or comet assay is a simple and sensitive method for quantitatively measuring DNA breakage and repair in individual cells. It can be applied to proliferating and non-proliferating cells and cells of those tissues, which are the first contact sites for mutagenic/carcinogenic substances. In this Technique, cells are embedded in agarose, lysed, subjected to Electrophoresis, and stained with a fluorescent DNA-binding dye. Cells with increased DNA damage display increased DNA migration from the nucleus toward the anode, which resembles the shape of a comet. The migration is observed by fluorescence microscopy after staining with a fluorescent DNA-binding dye, and the intensity of the comet tail reflects the number of DNA breaks. The assay is performed in almost all eukaryotic cells and has applications in many fields, including genetic toxicology, biomonitoring, ecotoxicology, medical, and nutritional research. The assay is a very sensitive tool to investigate the effect of carbon nanotubes on DNA of human cells in vitro. This chapter describes a procedure to perform the comet assay, in its alkaline version, on cell cultures treated with carbon nanotubes.

Wilhelm Nothdurft - One of the best experts on this subject based on the ideXlab platform.

  • Assessment of DNA damage in canine peripheral blood and bone marrow after total body irradiation using the single-cell gel Electrophoresis Technique.
    Mutation research, 1996
    Co-Authors: Ludwika Kreja, Christoph Selig, Wilhelm Nothdurft
    Abstract:

    DNA damage in single peripheral blood (pb) and bone marrow (bm) cells was studied in dogs which were exposed to total body X-ray irradiation (TBI) with a lethal dose of 3.9 Gy. The changes in pb and bm cell numbers were measured within 9 days after TBI. Using the alkaline single-cell gel Electrophoresis Technique ('comet' assay), DNA strand breaks and alkali labile sites were assessed in single cells derived from the blood before TBI, 1 h and 4 h after TBI and on days 1, 3 and 9 after TBI. Bone marrow cells subjected to the assay were collected before and on days 1 and 9 after TBI. Cells expressing the strongest DNA damage were most frequent in the blood 1 h after TBI and in the bone marrow 1 day after exposure. Thereafter, a continuous reduction of DNA damage in individual cells was observed in the course of progressive leukopenia and granulocytopenia.

S. E. Elmofty - One of the best experts on this subject based on the ideXlab platform.