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Min Zhang - One of the best experts on this subject based on the ideXlab platform.

  • changes of serum cholesterol high density lipoprotein and serum lipoprotein Electrophoretogram in senior patients with cerebral infarction
    Academic journal of the first medical college of PLA, 2003
    Co-Authors: Jia Yin, Xin-yu Wang, Min Zhang
    Abstract:

    OBJECTIVE To investigate the changes in the levels of serum cholesterol and high-density lipoprotein and in serum lipoprotein Electrophoretogram in elderly patients with cerebral infarction. METHODS Serum lipoprotein Electrophoretograms (SLPG) of 118 elderly patients with cerebral infarction and 60 healthy elderly subjects were measured by means of double gradient polyacrylamide gel electrophoresis, and the serum concentrations of total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) were determined by enzymatic method. RESULTS Compared with the control group, the serum level of HDL-C was significantly increased in the patients (1.0+/-0.3 mmol/L), who also had decreased TC/HDL-C ratio (5.3+/-1.4), but no significant difference occurred in terms of TC concentration between these two groups. Among the patients with cerebral infarction, 86.4% had abnormalities of SLPG, especially in the alpha-lipoprotein Electrophoretogram (P<0.001-0.05) regardless of HDL-C elevations. CONCLUSIONS Decreased HDL-C, elevated TC/HDL-C ratio and abnormal SLPG may be the risk factors for the onset of cerebral infarction in senior patients, for which alpha-lipoprotein Electrophoretogram can be more sensitive than serum HDL-C level. TC levels is of little value in predicting cerebral infarction.

  • Changes of serum cholesterol, high-density lipoprotein and serum lipoprotein Electrophoretogram in senior patients with cerebral infarction
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Jia Yin, Xin-yu Wang, Min Zhang
    Abstract:

    OBJECTIVE To investigate the changes in the levels of serum cholesterol and high-density lipoprotein and in serum lipoprotein Electrophoretogram in elderly patients with cerebral infarction. METHODS Serum lipoprotein Electrophoretograms (SLPG) of 118 elderly patients with cerebral infarction and 60 healthy elderly subjects were measured by means of double gradient polyacrylamide gel electrophoresis, and the serum concentrations of total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) were determined by enzymatic method. RESULTS Compared with the control group, the serum level of HDL-C was significantly increased in the patients (1.0+/-0.3 mmol/L), who also had decreased TC/HDL-C ratio (5.3+/-1.4), but no significant difference occurred in terms of TC concentration between these two groups. Among the patients with cerebral infarction, 86.4% had abnormalities of SLPG, especially in the alpha-lipoprotein Electrophoretogram (P

  • Features of serum beta-lipoprotein Electrophoretogram in patients with coronary heart disease.
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin
    Abstract:

    OBJECTIVE To investigate the changes in serum beta-lipoprotein (beta Lp) Electrophoretogram in the patients with coronary heart disease (CHD). METHODS This study included 36 CHD patients, 28 non-CHD patients and 8 normal control subjects, from whom serum beta Lps (prestained with Sudan Black B) were isolated by means of the first double-gradient polyacrylamide gel electrophoresis (f-DGPAGE). The secondary DGPAGE was performed for isolating beta Lps into 4 subfractions (beta Lp1-4) in order to complete the quantitative analysis of beta Lp Electrophoretogram. A parallel test to examine the serum concentration of low-density lipoprotein cholesterol (LDLc) was also carried out. RESULTS The relative amount of beta Lp1 was significantly elevated in CHD patients (53.4+/-10.0%) as compared with normal subjects (39.3+/-6.7%) and non-CHD patients (42.8+/-6.8%, P < 0.05 for both comparisons) regardless of serum LDLc concentrations, and beta Lp1 content between the latter 2 groups showed no significant difference. CONCLUSION Serum beta Lp subfraction imbalance evidenced by increased beta Lp1 level characterizes the patients with CHD.

  • A new method for detecting serum beta-lipoprotein Electrophoretogram
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin
    Abstract:

    OBJECTIVE To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE). METHODS After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the Electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE. RESULTS s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified. CONCLUSION s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.

Jia Yin - One of the best experts on this subject based on the ideXlab platform.

  • changes of serum cholesterol high density lipoprotein and serum lipoprotein Electrophoretogram in senior patients with cerebral infarction
    Academic journal of the first medical college of PLA, 2003
    Co-Authors: Jia Yin, Xin-yu Wang, Min Zhang
    Abstract:

    OBJECTIVE To investigate the changes in the levels of serum cholesterol and high-density lipoprotein and in serum lipoprotein Electrophoretogram in elderly patients with cerebral infarction. METHODS Serum lipoprotein Electrophoretograms (SLPG) of 118 elderly patients with cerebral infarction and 60 healthy elderly subjects were measured by means of double gradient polyacrylamide gel electrophoresis, and the serum concentrations of total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) were determined by enzymatic method. RESULTS Compared with the control group, the serum level of HDL-C was significantly increased in the patients (1.0+/-0.3 mmol/L), who also had decreased TC/HDL-C ratio (5.3+/-1.4), but no significant difference occurred in terms of TC concentration between these two groups. Among the patients with cerebral infarction, 86.4% had abnormalities of SLPG, especially in the alpha-lipoprotein Electrophoretogram (P<0.001-0.05) regardless of HDL-C elevations. CONCLUSIONS Decreased HDL-C, elevated TC/HDL-C ratio and abnormal SLPG may be the risk factors for the onset of cerebral infarction in senior patients, for which alpha-lipoprotein Electrophoretogram can be more sensitive than serum HDL-C level. TC levels is of little value in predicting cerebral infarction.

  • Changes of serum cholesterol, high-density lipoprotein and serum lipoprotein Electrophoretogram in senior patients with cerebral infarction
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Jia Yin, Xin-yu Wang, Min Zhang
    Abstract:

    OBJECTIVE To investigate the changes in the levels of serum cholesterol and high-density lipoprotein and in serum lipoprotein Electrophoretogram in elderly patients with cerebral infarction. METHODS Serum lipoprotein Electrophoretograms (SLPG) of 118 elderly patients with cerebral infarction and 60 healthy elderly subjects were measured by means of double gradient polyacrylamide gel electrophoresis, and the serum concentrations of total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) were determined by enzymatic method. RESULTS Compared with the control group, the serum level of HDL-C was significantly increased in the patients (1.0+/-0.3 mmol/L), who also had decreased TC/HDL-C ratio (5.3+/-1.4), but no significant difference occurred in terms of TC concentration between these two groups. Among the patients with cerebral infarction, 86.4% had abnormalities of SLPG, especially in the alpha-lipoprotein Electrophoretogram (P

  • Features of serum beta-lipoprotein Electrophoretogram in patients with coronary heart disease.
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin
    Abstract:

    OBJECTIVE To investigate the changes in serum beta-lipoprotein (beta Lp) Electrophoretogram in the patients with coronary heart disease (CHD). METHODS This study included 36 CHD patients, 28 non-CHD patients and 8 normal control subjects, from whom serum beta Lps (prestained with Sudan Black B) were isolated by means of the first double-gradient polyacrylamide gel electrophoresis (f-DGPAGE). The secondary DGPAGE was performed for isolating beta Lps into 4 subfractions (beta Lp1-4) in order to complete the quantitative analysis of beta Lp Electrophoretogram. A parallel test to examine the serum concentration of low-density lipoprotein cholesterol (LDLc) was also carried out. RESULTS The relative amount of beta Lp1 was significantly elevated in CHD patients (53.4+/-10.0%) as compared with normal subjects (39.3+/-6.7%) and non-CHD patients (42.8+/-6.8%, P < 0.05 for both comparisons) regardless of serum LDLc concentrations, and beta Lp1 content between the latter 2 groups showed no significant difference. CONCLUSION Serum beta Lp subfraction imbalance evidenced by increased beta Lp1 level characterizes the patients with CHD.

  • A new method for detecting serum beta-lipoprotein Electrophoretogram
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin
    Abstract:

    OBJECTIVE To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE). METHODS After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the Electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE. RESULTS s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified. CONCLUSION s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.

Bing-sheng Yin - One of the best experts on this subject based on the ideXlab platform.

  • Features of serum beta-lipoprotein Electrophoretogram in patients with coronary heart disease.
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin
    Abstract:

    OBJECTIVE To investigate the changes in serum beta-lipoprotein (beta Lp) Electrophoretogram in the patients with coronary heart disease (CHD). METHODS This study included 36 CHD patients, 28 non-CHD patients and 8 normal control subjects, from whom serum beta Lps (prestained with Sudan Black B) were isolated by means of the first double-gradient polyacrylamide gel electrophoresis (f-DGPAGE). The secondary DGPAGE was performed for isolating beta Lps into 4 subfractions (beta Lp1-4) in order to complete the quantitative analysis of beta Lp Electrophoretogram. A parallel test to examine the serum concentration of low-density lipoprotein cholesterol (LDLc) was also carried out. RESULTS The relative amount of beta Lp1 was significantly elevated in CHD patients (53.4+/-10.0%) as compared with normal subjects (39.3+/-6.7%) and non-CHD patients (42.8+/-6.8%, P < 0.05 for both comparisons) regardless of serum LDLc concentrations, and beta Lp1 content between the latter 2 groups showed no significant difference. CONCLUSION Serum beta Lp subfraction imbalance evidenced by increased beta Lp1 level characterizes the patients with CHD.

  • A new method for detecting serum beta-lipoprotein Electrophoretogram
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin
    Abstract:

    OBJECTIVE To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE). METHODS After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the Electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE. RESULTS s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified. CONCLUSION s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.

Yin Bing-sheng - One of the best experts on this subject based on the ideXlab platform.

Yun-qin Chen - One of the best experts on this subject based on the ideXlab platform.

  • Features of serum beta-lipoprotein Electrophoretogram in patients with coronary heart disease.
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin
    Abstract:

    OBJECTIVE To investigate the changes in serum beta-lipoprotein (beta Lp) Electrophoretogram in the patients with coronary heart disease (CHD). METHODS This study included 36 CHD patients, 28 non-CHD patients and 8 normal control subjects, from whom serum beta Lps (prestained with Sudan Black B) were isolated by means of the first double-gradient polyacrylamide gel electrophoresis (f-DGPAGE). The secondary DGPAGE was performed for isolating beta Lps into 4 subfractions (beta Lp1-4) in order to complete the quantitative analysis of beta Lp Electrophoretogram. A parallel test to examine the serum concentration of low-density lipoprotein cholesterol (LDLc) was also carried out. RESULTS The relative amount of beta Lp1 was significantly elevated in CHD patients (53.4+/-10.0%) as compared with normal subjects (39.3+/-6.7%) and non-CHD patients (42.8+/-6.8%, P < 0.05 for both comparisons) regardless of serum LDLc concentrations, and beta Lp1 content between the latter 2 groups showed no significant difference. CONCLUSION Serum beta Lp subfraction imbalance evidenced by increased beta Lp1 level characterizes the patients with CHD.

  • A new method for detecting serum beta-lipoprotein Electrophoretogram
    Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, 2003
    Co-Authors: Yun-qin Chen, Jia Yin, Min Zhang, Bing-sheng Yin
    Abstract:

    OBJECTIVE To establish a new method for isolating serum beta-lipoprotein (betaLp) subfractions using second double gradient polyacrylamide gel electrophoresis (s-DGPAGE). METHODS After the betaLp subfractions were isolated by mechanisms of molecular sieving and isoelectric focusing due to density and pH grade in the non-linear density gradient polyacrylamide gel, the gels were acquired with a gel imaging system, the Electrophoretograms were quantitatively analyzed with optical density scanner. The isolation of serum betaLp was completed through first DGPAGE. RESULTS s-DGPAGE separated the serum betaLp into four subfractions, designated respectively as betaLp1, betaLp2, betaLp3 and betaLp4, and their amounts in the betaLp and in the whole lipoproteins could be quantified. CONCLUSION s-DGPAGE is a simple and practical method to show the heterogeneity of serum betaLp and the quantitative variation of its subfractions.