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Seeram Ramakrishna - One of the best experts on this subject based on the ideXlab platform.
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protein encapsulated core shell structured particles prepared by coaxial Electrospraying investigation on material and processing variables
International Journal of Pharmaceutics, 2014Co-Authors: Maedeh Zamani, Molamma P Prabhakaran, Eng San Thian, Seeram RamakrishnaAbstract:Abstract Biodegradable polymeric particles have been extensively investigated for controlled drug delivery of various therapeutic agents. ‘Coaxial’ Electrospraying was successfully employed in this study, to fabricate core–shell PLGA particles containing bovine serum albumin (BSA) as the model protein, and the results were also compared to particles prepared by ‘emulsion’ Electrospraying. Two different molecular weights of PLGA were employed to encapsulate the protein. Solution properties and processing parameters were found to influence the morphology of the core–shell particles. Depending on the type of solvent used to dissolve the polymer as well as the polymer concentration and molecular weight, the mean diameter of the particles varied between 3.0 to 5.5 μm. Fluorescence microscopic analysis of the electrosprayed particles using FITC-conjugated BSA demonstrated the core–shell structure of the developed particles. The encapsulation efficiency and release behavior of BSA was influenced by shell:core feeding ratio, protein concentration, and the Electrospraying method. The encapsulation efficiency of BSA within the core–shell particles of high and low molecular weight PLGA was found 15.7% and 25.1% higher than the emulsion electrosprayed particles, respectively. Moreover, the total amount of BSA released from low molecular weight PLGA particles was significantly higher than high molecular weight PLGA particles within 43 days of release studies, with negligible effect on encapsulation efficiency. The technique of coaxial Electrospraying has high potential for encapsulation of susceptible protein-based therapeutic agents such as growth factors for multiple drug delivery applications.
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Electrosprayed nanoparticles for drug delivery and pharmaceutical applications
Biomatter, 2013Co-Authors: Radhakrishnan Sridhar, Seeram RamakrishnaAbstract:Nanotechnology based Pharma has emerged significantly and has influenced the Pharma industry up to a considerable extent. Nanoparticles technology holds a good share of the nanotech Pharma and is significant in comparison with the other domains. Electrospraying technology answers the potential needs of nanoparticle production such as scalability, reproducibility, effective encapsulation etc. Many drugs have been electrosprayed with and without polymer carriers. Drug release characteristics are improved with the incorporation of biodegradable polymer carriers which sustain the release of encapsulated drug. Electrospraying is acknowledged as an important technique for the preparation of nanoparticles with respect to pharmaceutical applications. Herein we attempted to consolidate the reports pertaining to Electrospraying and their corresponding therapeutic application area.
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Electrospun polyimide/titanium dioxide composite nanofibrous membrane by electrospinning and Electrospraying.
Journal of Nanoscience and Nanotechnology, 2011Co-Authors: Lijo F, Enrico Marsano, Chellappan Vijila, R.s. Barhate, Vijay Vk, Seeram Ramakrishna, ThavasiAbstract:Fibrous membrane with a fibre diameter of 229 +/- 35 nm was fabricated from polyimide solution by electrospinning. Nanofibrous membrane with a fibre diameter of 251 +/- 37 nm was fabricated by combined electrospinning and Electrospraying for polyimide/TiO2. Among the different solvents studied, ethanol was the effective solvent for dispersing the TiO2 nanoparticles in the nanofibrous matrix during Electrospraying. The average pore size of polyimide membrane was obtained in the range 0.79-0.89 microm whereas the average pore size of polyimide/TiO2 membrane was found to be in the range 1.23 microm. The tensile stress of polyimide nanofibrous membrane and also polyimide/TiO2 composite fibrous membrane determined to be 0.36 MPa and 0.65 MPa respectively. Nanofibrous membrane containing TiO2 nanoparticles on the surface of the polyimide nanofibres improved the mechanical stability of the membrane.
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nanocomposite fabric formation by electrospinning and Electrospraying technologies
Journal of Electrostatics, 2009Co-Authors: A. Jaworek, M. Lackowski, Alexandre Krupa, A T Sobczyk, Thomas Czech, Subramanian Sundarrajan, Seeram Ramakrishna, D. PliszkaAbstract:Abstract Electrospraying and electrospinning processes were employed for the production of nanocomposite material of polymer nanofibers blended with nanoparticles. The diameter of polymer nanofibers made of PVC, PSU or nylon was smaller than 500 nm. Metal oxide nanoparticles of TiO 2 , MgO, and Al 2 O 3 of the size 20–100 nm suspended in methanol were deposited on the polymer nanofibers. Three configurations of electrospray/electrospun nozzles used for the nanocomposite production were tested: 1. simultaneous Electrospraying during the electrospinning process, 2. Electrospraying onto the same rotating drum after the electrospinning is completed, and 3. Electrospraying onto the electrospun mat removed from the drum and placed onto a heated table.
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nanostructured biocomposite substrates by electrospinning and Electrospraying for the mineralization of osteoblasts
Biomaterials, 2009Co-Authors: Deepika Gupta, Jayarama Reddy Venugopal, S. Mitra, Seeram RamakrishnaAbstract:Abstract Nanotechnology has enabled the engineering of nanostructured materials to meet current challenges in bone replacement therapies. Biocomposite nanofibrous scaffolds of poly( l -lactic acid)- co -poly( ɛ -caprolactone), gelatin and hydroxyapatite (HA) were fabricated by combining the electrospinning and Electrospraying techniques in order to create a better osteophilic environment for the growth and mineralization of osteoblasts. Electrospraying of HA nanoparticles on electrospun nanofibers helped to attain rough surface morphology ideal for cell attachment and proliferation and also achieve improved mechanical properties than HA blended nanofibers. Nanofibrous scaffolds showed high pore size and porosity up to 90% with fiber diameter in the range of 200–700 nm. Nanofibrous scaffolds were characterized for their functional groups and chemical structure by FTIR and XRD analysis. Studies on cell–scaffold interaction were carried out by culturing human fetal osteoblast cells (hFOB) on both HA blended and sprayed PLACL/Gel scaffolds and assessing their growth, proliferation, mineralization and enzyme activity. The results of MTS, ALP, SEM and ARS studies confirmed, not only did HA sprayed biocomposite scaffolds showed better cell proliferation but also enhanced mineralization and alkaline phosphatase activity (ALP) proving that Electrospraying in combination with electrospinning produced superior and more suitable biocomposite nanofibrous scaffolds for bone tissue regeneration.
Dengguang Yu - One of the best experts on this subject based on the ideXlab platform.
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immediate release of helicid from nanoparticles produced by modified coaxial Electrospraying
Applied Surface Science, 2019Co-Authors: Dengguang Yu, Yaoyao Yang, Xl Zheng, Xiaoyan Li, Gareth R Williams, Min ZhaoAbstract:Abstract In this paper, a modified coaxial Electrospraying process was explored for the generation of novel nanoscale composite materials. A solution comprising 5% (w/v) of the model drug helicid and 10% (w/v) polyvinylpyrrolidone (PVP) K10 in a mixture of N,N-dimethylacetamide and ethanol (4:6, v:v) was employed as the shell working liquid. This could not be processed into a solid product when processed by single-fluid Electrospraying. However, when undertaking co-axial Electrospraying with a core shellac solution (40% w/v in ethanol) solid particles were obtained. An extremely thin nanocoating layer of drug-polymer composite with an estimated thickness of 7 nm was deposited on a shellac core. X-ray diffraction and infrared spectroscopy demonstrated that the drug was converted into an amorphous nanocomposite with the PVP in the shell layer, losing its original crystalline state. In vitro dissolution tests revealed that all the helicid loading could be released within one minute, suggesting the particles have potential applications to deliver very rapid therapeutic effects. Mechanisms are proposed underlying the formation and functional performance of the materials.
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meletin sustained release gliadin nanoparticles prepared via solvent surface modification on blending Electrospraying
Applied Surface Science, 2018Co-Authors: Yaoyao Yang, Man Zhang, Ke Wang, Dengguang YuAbstract:Abstract Almost all Electrospraying processes are carried out under an air-solution interface, thereby overlooking the potential influence of an additional solvent surface modification between air and the working solution. A pure solvent was explored to temporarily and dynamically surround the solutions utilized for blending Electrospraying, which contained a guest drug meletin and a protein drug carrier gliadin. The new modified processes created protein-based medicated nanoparticles (P2) with higher quality than their counterparts (P1) from blending processes, as demonstrated by the SEM and TEM images. Although the particles from the two processes were similar (nanocomposites), and the particles P1 were larger than P2, the later provided a better meletin sustained-release profile than the former. This finding was verified by the smaller initial burst release, longer sustained-release time period, and shorter late leveling-off stage. These unanticipated results were attributed to the rounder surface, the more uniform size distribution, and the smaller total surface area of particles P2 than P1. The microformation mechanism of the modified coaxial process was suggested. The protocols reported here paved a new way for the development of new kinds of functional nanoparticles by modifying the interfaces of working fluids during Electrospraying.
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preparing composite nanoparticles for immediate drug release by modifying electrohydrodynamic interfaces during Electrospraying
Powder Technology, 2018Co-Authors: Lingling Zhang, Di Wu, Yaoyao Yang, Ge Jiang, Dengguang YuAbstract:Abstract At present, all Electrospraying processes are conducted in air environment without considering the importance of electrohydrodynamic interfaces, thereby preventing the development of new possibilities. In this study, a modified coaxial Electrospraying process for preparing multicomponent composite nanoparticles was investigated, in which solvents were explored as the shell working fluids to create dynamic air–solvent solution interfaces. The coaxial process was conducted using a blending solution (comprising hydroxypropyl methylcellulose, paracetamol (APAP), and polyvinylpyrrolidone in a mixture of dichloromethane and ethanol) as the core fluid and a solvent mixture as the shell fluid under a series of shell-to-core fluid flow rate ratios. Results demonstrated that the interface modification by the additional shell solvent generated significant influences on the final particulate multicomponent composites. An exponential relationship between the shell-to-core fluid flow rate ratio (F) and the particle diameters (D, μm) could be determined as D = 0.54 F− 0.39 (| R | = 0.9890). APAP was amorphously distributed within its solid “cocktails” to form a multicomponent nanocomposite. The resultant particles from the modified coaxial process could provide an immediate drug release, with a best value of four times faster than the counterparts from traditional blending Electrospraying. The synergistic actions of particle size, amorphous drug status, and combined usage of hydrophilic polymers could ensure the fast dissolution and immediate release of APAP. The protocols reported in this study paved a new way for developing new types of functional nanomaterials via coaxial Electrospraying.
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fabrication of sustained release zein nanoparticles via modified coaxial Electrospraying
Chemical Engineering Journal, 2018Co-Authors: Yaoyao Zhang, Dengguang Yu, Di Wu, Haolin LiAbstract:Abstract A modified coaxial Electrospraying process, which was characterized by the use of pure solvent as a sheath working fluid, was developed for the continuous and robust generation of medicated nanoparticles. Tamoxifen citrate (TAM) was encapsulated in zein, a plant protein. The influences of the key parameter shell-to-core fluid flow rate on the Electrospraying processes and on the resulting protein-based composite particles were investigated in detail. Scanning electron microscopy images revealed that compared with particles prepared via the traditional single-fluid process, TAM-loaded zein particles prepared via the modified coaxial process were rounder with smaller diameters, narrower smaller size distribution, and more compact inner structures. Owing to the reinforced secondary interactions between TAM and zein, all the particles were similarly amorphous composites, as verified by their X-ray patterns and attenuated total reflectance–Fourier transform infrared spectra. The medicated nanoparticles synthesized via the coaxial process exhibited a better drug sustained-release profile than those synthesized via the traditional single-fluid process, with a smaller initial burst release, longer release period, and shorter tailing-off stage. The microformation mechanisms of the electrosprayed particles and their drug sustained-release mechanisms were suggested. By altering the traditional concept of Electrospraying, the modified coaxial Electrospraying method developed in this study provides a powerful new process for the generation of nanoparticles with novel structures and functions.
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sustained release of ethyl cellulose micro particulate drug delivery systems prepared using Electrospraying
Journal of Materials Science, 2012Co-Authors: Liya Huang, Dengguang Yu, Christopher Branfordwhite, Limin ZhuAbstract:Sustained-release ethyl cellulose (EC) micro-particles were prepared by Electrospraying. Ketoprofen (KET) was taken as a model drug and various concentrations of EC functioned as a rate-controlling polymer. The morphology of the micro-particles was assessed using SEM. Images showed that as EC content increased, the granules shared similar surface characteristics containing pure EC. Micro-particle structures were analyzed by DSC, XRD, and FTIR. It was noted that the crystalline drug was converted into an amorphous form in all the granulations and that there was chemical interaction between KET and EC observed from FTIR. Dissolution studies revealed that as the amount of EC increased, the drug release rate decreased. This investigation suggests that Electrospraying can be exploited as a useful tool for developing novel particulate drug delivery systems.
Guy Van Den Mooter - One of the best experts on this subject based on the ideXlab platform.
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Feasibility of Electrospraying fully aqueous bovine serum albumin solutions
European Journal of Pharmaceutics and Biopharmaceutics, 2019Co-Authors: Maarten Batens, Loís Dewaele, Jan Massant, Bianca Teodorescu, Christian Clasen, Guy Van Den MooterAbstract:Abstract Electrospraying or electrohydrodynamic atomisation, i.e. the formation of tiny droplets from a jet of conductive liquid under the influence of an electric field, has been gaining in popularity as a particle engineering technique in recent years. In addition to general benefits for particle engineering, e.g. the ability to generate nanometre sized particles with a very narrow size distribution, Electrospraying also possesses a number of characteristics, like its applicability at ambient conditions, which could make it especially interesting for formulating therapeutic proteins. However, as fully aqueous solutions of proteins tend to have relatively high electrical conductivities and surface tensions, obtaining a stable Taylor cone-jet mode for these solutions is inherently challenging. This is why in the majority of studies reporting the successful Electrospraying of proteins, either emulsions, aqueous suspensions or a mixture of water and one or more organic solvents were used instead of fully aqueous solutions. Therefore, an ab initio Electrospraying formulation development study was conducted, using only fully aqueous feed solutions containing protein stabilising excipients commonly used in spray- and freeze-drying of therapeutic proteins. The study included bovine serum albumin (BSA) as a model protein and consisted out of two parts: (1) a one parameter at a time screening study, designed to improve the understanding of how various formulation components influence relevant physicochemical properties and the Electrospraying process and (2) two subsequent mixture design of experiments (DoE) studies, designed to aid in the statistical description and prediction of the influence of different protein-excipient combinations on the Electrospraying process. Additionally, the influence of physicochemical properties relevant to the Electrospraying process, i.e. the volumetric mass density, electrical conductivity, kinematic viscosity and surface tension, was assessed for all feed solutions included in the study.
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Electrospraying of polymer solutions study of formulation and process parameters
European Journal of Pharmaceutics and Biopharmaceutics, 2017Co-Authors: Annelies Smeets, Christian Clasen, Guy Van Den MooterAbstract:Abstract Over the past decade, Electrospraying has proven to be a promising method for the preparation of amorphous solid dispersions, an established formulation strategy to improve the oral bioavailability of poorly soluble drug compounds. Due to the lack of fundamental knowledge concerning adequate single nozzle Electrospraying conditions, a trial-and-error approach is currently the only option. The objective of this paper is to study/investigate the influence of the different formulation and process parameters, as well as their interplay, on the formation of a stable cone-jet mode as a prerequisite for a reproducible production of monodisperse micro- and nanoparticles. To this purpose, different polymers commonly used in the formulation of solid dispersions were electrosprayed to map out the workable parameter ranges of the process. The experiments evaluate the importance of the experimental parameters as flow rate, electric potential difference and the distance between the tip of the nozzle and collector. Based on this, the type of solvent and the concentration of the polymer solutions, along with their viscosity and conductivity, were identified as determinative formulation parameters. This information is of utmost importance to rationally design further Electrospraying methods for the preparation of amorphous solid dispersions.
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pharmaceutical applications of Electrospraying
Journal of Pharmaceutical Sciences, 2016Co-Authors: Duong Nhat Nguyen, Christian Clasen, Guy Van Den MooterAbstract:Abstract The electrohydrodynamic atomization technique, or simply called Electrospraying, has been extensively studied for biomedical as well as for pharmaceutical applications over the past years. The simplicity, flexibility, and efficiency of producing particles at the microscale or nanoscale, with tailored size, shape, morphology, and microstructure, make Electrospraying to become one of the most promising and well-practiced approaches to be applied in many biomedical and pharmaceutical fields, from improving the bioavailability of poorly aqueous soluble drugs, preparing targeted drug delivery systems, and controllable drug release systems to delivering sensitive therapeutic agents such as protein-based drugs or even living cells. Nevertheless, some issues still remain with respect to low throughput as well as the complex interplay between a great number of processing and formulation factors. A comprehensive understanding of these fundamental aspects is essential for the successful application of Electrospraying for the production of particulate formulations with desired properties.
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one step production of darunavir solid dispersion nanoparticles coated with enteric polymers using Electrospraying
Journal of Pharmacy and Pharmacology, 2016Co-Authors: Duong Nhat Nguyen, Christian Clasen, Ljiljana Palangetic, Guy Van Den MooterAbstract:Objectives The aim of this work was to investigate the feasibility of producing darunavir (DRV) solid dispersion nanoparticles coated with an enteric polymer in one single step using Electrospraying. Methods The core-shell nanoparticles were made using coaxial Electrospraying. A solution of DRV with hydroxypropyl methylcellulose in a mixture of organic solvents formed the core, while the shell was produced from an enteric polymer (Eudragit L100) dissolved in an organic solvent. The final particles were evaluated in terms of morphology, physical state, encapsulation efficiency and in-vitro dissolution. Key findings Nanoparticles of encapsulated DRV solid dispersions within Eudragit L100 were successfully prepared with high encapsulation efficiency (90%). The enteric coating layer reduced the percentage of DRV release in acidic medium in the in-vitro dissolution test to less than 20%. Conclusions This study showed the potential of coaxial Electrospraying for encapsulating solid dispersions within core-shell structured nanoparticles.
M. J. Edirisinghe - One of the best experts on this subject based on the ideXlab platform.
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improved mechanical reliability of bone tissue engineering zirconia scaffolds by Electrospraying
Journal of the American Ceramic Society, 2006Co-Authors: Qizhi Chen, Aldo Roberto Boccaccini, H. B. Zhang, Dezhen Wang, M. J. EdirisingheAbstract:A novel process, namely replication combined with Electrospraying, was used to fabricate bioceramic (zirconia) scaffolds with the intention of improving the mechanical function of hard tissue engineering scaffolds. A comparative study was carried out on the microstructure and mechanical properties of zirconia foams prepared by this new process or by the conventional slurry-dip coating method. After forming the green bodies, they were subjected to a sintering heat treatment at 1450°C for 5 h. It was found that the number and size of microcracks and micropores can be reduced in foams prepared by Electrospraying in comparison with those prepared by slurry-dip coating. The values of compressive mechanical strength and Young's modulus were found to be collectively higher for scaffolds prepared by Electrospraying than for those prepared by slurry-dip coating. Moreover, the mechanical reliability was improved in foams produced by the Electrospraying technique. The improvement is attributable to the reduction in the population and size of microcracks and micropores.
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Improved mechanical reliability of bone tissue engineering (Zirconia) scaffolds by Electrospraying
J AM CERAM SOC, 2006Co-Authors: M. J. EdirisingheAbstract:A novel process, namely replication combined with Electrospraying, was used to fabricate bioceramic (zirconia) scaffolds with the intention of improving the mechanical function of hard tissue engineering scaffolds. A comparative study was carried out on the microstructure and mechanical properties of zirconia foams prepared by this new process or by the conventional slurry-dip coating method. After forming the green bodies, they were subjected to a sintering heat treatment at 1450 degrees C for 5 h. It was found that the number and size of microcracks and micropores can be reduced in foams prepared by Electrospraying in comparison with those prepared by slurry-dip coating. The values of compressive mechanical strength and Young's modulus were found to be collectively higher for scaffolds prepared by Electrospraying than for those prepared by slurry-dip coating. Moreover, the mechanical reliability was improved in foams produced by the Electrospraying technique. The improvement is attributable to the reduction in the population and size of microcracks and micropores.
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Improved mechanical reliability of bone tissue engineering (zirconia) scaffolds by Electrospraying
Journal of the American Ceramic Society, 2006Co-Authors: Q Z Chen, D.z. Wang, H. B. Zhang, Aldo Roberto Boccaccini, M. J. EdirisingheAbstract:A novel process, namely replication combined with eleetrospraying, was used to fabricate bioceramic (zirconia) scaffolds with the intention of improving the mechanical function of hard tissue engineering scaffolds. A comparative study was carried out on the niicrostructure and mechanical properties of zirconia foams prepared by this new process or by the conventional slurry-dip coating method. After forming the green bodies, they were subjected to a sintering heat treatment at 1450°C for 5 h. It was found that the number and size of microcracks and micropores can be reduced in foams prepared by Electrospraying in comparison with those prepared by slurry-dip coating. The values of compressive mechanical strength and Young's modulus were found to be collectively higher for scaffolds prepared by Electrospraying than for those prepared by slurry-dip coating. Moreover, the mechanical reliability was improved in foams produced by the Electrospraying technique. The improvement is attributable to the reduction in the population and size of microcracks and micropores. © 2006 The American Ceramic Soeiety.
Deepika Gupta - One of the best experts on this subject based on the ideXlab platform.
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nanostructured biocomposite substrates by electrospinning and Electrospraying for the mineralization of osteoblasts
Biomaterials, 2009Co-Authors: Deepika Gupta, Jayarama Reddy Venugopal, S. Mitra, Seeram RamakrishnaAbstract:Abstract Nanotechnology has enabled the engineering of nanostructured materials to meet current challenges in bone replacement therapies. Biocomposite nanofibrous scaffolds of poly( l -lactic acid)- co -poly( ɛ -caprolactone), gelatin and hydroxyapatite (HA) were fabricated by combining the electrospinning and Electrospraying techniques in order to create a better osteophilic environment for the growth and mineralization of osteoblasts. Electrospraying of HA nanoparticles on electrospun nanofibers helped to attain rough surface morphology ideal for cell attachment and proliferation and also achieve improved mechanical properties than HA blended nanofibers. Nanofibrous scaffolds showed high pore size and porosity up to 90% with fiber diameter in the range of 200–700 nm. Nanofibrous scaffolds were characterized for their functional groups and chemical structure by FTIR and XRD analysis. Studies on cell–scaffold interaction were carried out by culturing human fetal osteoblast cells (hFOB) on both HA blended and sprayed PLACL/Gel scaffolds and assessing their growth, proliferation, mineralization and enzyme activity. The results of MTS, ALP, SEM and ARS studies confirmed, not only did HA sprayed biocomposite scaffolds showed better cell proliferation but also enhanced mineralization and alkaline phosphatase activity (ALP) proving that Electrospraying in combination with electrospinning produced superior and more suitable biocomposite nanofibrous scaffolds for bone tissue regeneration.
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Nanostructured biocomposite substrates by electrospinning and Electrospraying for the mineralization of osteoblasts
Biomaterials, 2009Co-Authors: Deepika Gupta, V. R. Giri Dev, Jayarama Reddy Venugopal, J. Venugopal, S. Mitra, Seeram RamakrishnaAbstract:Nanotechnology has enabled the engineering of nanostructured materials to meet current challenges in bone replacement therapies. Biocomposite nanofibrous scaffolds of poly(l-lactic acid)-co-poly(ε-caprolactone), gelatin and hydroxyapatite (HA) were fabricated by combining the electrospinning and Electrospraying techniques in order to create a better osteophilic environment for the growth and mineralization of osteoblasts. Electrospraying of HA nanoparticles on electrospun nanofibers helped to attain rough surface morphology ideal for cell attachment and proliferation and also achieve improved mechanical properties than HA blended nanofibers. Nanofibrous scaffolds showed high pore size and porosity up to 90% with fiber diameter in the range of 200-700 nm. Nanofibrous scaffolds were characterized for their functional groups and chemical structure by FTIR and XRD analysis. Studies on cell-scaffold interaction were carried out by culturing human fetal osteoblast cells (hFOB) on both HA blended and sprayed PLACL/Gel scaffolds and assessing their growth, proliferation, mineralization and enzyme activity. The results of MTS, ALP, SEM and ARS studies confirmed, not only did HA sprayed biocomposite scaffolds showed better cell proliferation but also enhanced mineralization and alkaline phosphatase activity (ALP) proving that Electrospraying in combination with electrospinning produced superior and more suitable biocomposite nanofibrous scaffolds for bone tissue regeneration. © 2008 Elsevier Ltd. All rights reserved.