The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Ekrem Gürel - One of the best experts on this subject based on the ideXlab platform.
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Gynogenesis Induction in Sugar Beet (Beta vulgaris) Improved by 6-Benzylaminopurine (BAP) and Synergized with Cold Pretreatment
Sugar Tech, 2018Co-Authors: Arman Pazuki, Fatemeh Aflaki, Ali Ergül, Ekrem Gürel, Songül GürelAbstract:For sugar beet ( Beta vulgaris ) breeding, producing homozygous lines through haploid and doubled haploid techniques are preferred over conventional and time-consuming methods. Doubled haploid sugar beet production necessitates inducing ovules to develop into haploid plants, referred to as gynogenesis. The protocol involves an interaction between cold pretreatment of six genotypes of sugar beet inflorescences at 4 °C for 1 week or more and 6-benzylaminopurine (BAP) concentrations (1 or 2 mg L^−1) to increase the response rate of haploid Embryo Induction. Compared with freshly cultured ovules (6.49%), cold pretreatment for 1 week almost doubled the mean of haploid plantlet Induction rate (11.3%), whereas pretreatment for more than 1 week was not as effective as the control. Addition of 2 mg L^−1 BAP to the culture medium nearly doubled the Induction rate of the cultured ovules (10.75%), followed by 1 mg L^−1 BAP (7.78%) in comparison with hormone-free medium (5.69%). The highest gynogenesis rate (37.8%) was achieved when ovules were cultured on medium containing 2 mg L^−1 BAP following 1-week cold pretreatment. This combination approximately tripled the mean total haploid Embryo Induction rate of all the genotypes to 16.3% in comparison with the control (5.74%). However, the addition of BAP resulted in vitrification proportionately. As a result, 2 mg L^−1 BAP decreased the normal plantlet emergence (NPE) to one-third (7.59%) while 1 mg L^−1 BAP had a moderate effect (NPE: 18.98%) in comparison with hormone-free treatment (NPE: 24.35%). The results indicate that the combination of cold pretreatment and BAP is very effective in inducing haploid plants from recalcitrant genotypes of sugar beet, but BAP can have both advantages and disadvantages.
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Production of doubled haploids in sugar beet ( Beta vulgaris ): an efficient method by a multivariate experiment
Plant Cell Tissue and Organ Culture (PCTOC), 2017Co-Authors: Arman Pazuki, Fatemeh Aflaki, Songül Gürel, Ali Ergül, Ekrem GürelAbstract:The present paper describes a detailed study of a highly efficient protocol to multiply the number of haploids in sugar beet production and subsequent chromosome doubling. The protocol involves an experiment investigating factorial interactions between cold pretreatment, seven genotypes of sugar beet, and kinetin to improve haploid Embryo Induction. In addition, the effects of color of ovules and flower bud position on haploid Embryo Induction were investigated. After subjecting the data to analysis of variance or Student’s t test (P < .05), the effect sizes of the independent variables were also estimated. Cold pretreatment was effective in stimulating the ovules. The haploid Embryo Induction rate for 1-week cold pretreated ovules (9.01%) was higher than that of freshly cultured ones (6.15%). In comparison with hormone-free medium (5.16%), the gynogenesis rate for the media supplemented with 0.05 or 0.5 mg L−l kinetin increased to 7.58 and 10.05%, respectively. The genotype responses were significantly different. Interactions of kinetin × cold pretreatment, genotype × hormonal treatment, genotype × cold pretreatment, and the three-way interaction were statistically significant. Moreover, the main effects of flower bud position, ovule color, and comma-form ovule on gynogenic response were significant. After investigating the effect of 5 g L−l colchicine for 3, 5, or 7 min on one genotype’s (SG2) specimens, all the haploid plantlets from the other genotypes were treated for 5 min as the best treatment. The paper discusses interactions of the factors, which may be interesting for others aiming to breed doubled haploid sugar beet or possibly other related plant species.
A Atanassov - One of the best experts on this subject based on the ideXlab platform.
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Embryo Induction and regeneration from root explants of medicago truncatula after osmotic pre treatment
Plant Cell Tissue and Organ Culture, 2005Co-Authors: Anelia Iantcheva, Slavtcho Slavov, Els Prinsen, Mariana Vlahova, Henry Van Iantcheva, Henry Van Onckelen, A AtanassovAbstract:Embryo Induction and regeneration from suspension culture of two Medicago truncatula cvs. (cv. R 108 1 and cv. Jemalong) have been studied. The influence of osmotic pre-treatment (1 M solution of sucrose for 48 h and 72 h) of roots as an initial explant, on Embryogenic efficiency of the suspension culture was assessed. In comparison to the control, the level of abscisic acid (ABA) increased significantly after osmotic stress. The increased ABA level did not correlate with the Induction of Embryogenesis neither with the improved Embryogenic potential of cv. R 108 1. The shortest regeneration period and the highest percent of conversion to plants were found in cv. R 108 1 after 72-h pre-treatment of roots. The efficiency of somatic Embryo conversion was less after 48-h pre-treatment and much less for the untreated control. Osmotic stress did not positively affect the process of Embryogenesis from root explants of cv. Jemalong, confirming its cultivar dependence. A single cell suspension fraction was produced in both Medicago trunacatula cvs. during the somatic Embryo maturation stage. A higher Embryogenic potential than the initial suspension culture was established only for the cell suspension originating from 72-h pre-treated roots of cv. R 108 1. The data confirms that the process of somatic Embryo Induction and Embryo conversion from root explants of cv. R 108 1 could be promoted by osmotic stress pre-treatment.
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Somatic Embryogenesis of Rosa Hybrida L.
Biotechnology & Biotechnological Equipment, 2000Co-Authors: A. Borissova, V. Tsolova, V. Angeliev, A AtanassovAbstract:ABSTRACTWe present two regeneration protocols via somatic Embryogenesis in three cut roses—Rosa hybrida cvs. “Anny”, “Trimontsium” and “Saltze Gold”. The first regeneration protocols are based on regeneration via somatic Embryogenesis in callus from leaf and petiole segments of Rosa hybrida cvs. “Trimontsium” and “Saltze Gold”. The other protocol is via somatic Embryogenesis in callus culture from petals of Rosa hybrida cv. “Anny”. Both regeneration protocols were developed on solid medium under subculturing on every 35–40 days. MS-medium including MS (1962—Murashige & Skoog) or QL (Quorin & Lepoivre) vitamins were used. For the Embryo Induction of callus derived from leaf and petiole explants were used BAP, NAA and kinetin, while for the combination of dicamba, kinetin and 2-ip are the most efficient for the Embryo Induction of callus derived from petals. A part of these Embryos was converted to intact plants and an other part had been multiplicated and maintained in vitro for two years in order to check...
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Microspore Culture of Winter Oil Rapeseed (Brassica Napus): I. Effect of Culture Conditions on Embryo Formation from Microspores in Winter Oil Rapeseed
Biotechnology & Biotechnological Equipment, 1997Co-Authors: A. Trifonova, A AtanassovAbstract:ABSTRACTEmbryogenie response of rapeseed microspore culture from ten hybrid combinations have been evaluated. The frequency of Embryo formation was strongly influenced by genotype. Bud size correlated with microspore stage of development has been determined for any of studied genotype. Embryo formation occur when microspores are predominantly in late uninucleate stage of development. Our experiments suggest that optimal density for Embryo Induction is between 40000 to 60000 microspore per ml. The increased Embryo Induction rate have been achieved when growth hormones were included in culture media.
Arman Pazuki - One of the best experts on this subject based on the ideXlab platform.
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Gynogenesis Induction in Sugar Beet (Beta vulgaris) Improved by 6-Benzylaminopurine (BAP) and Synergized with Cold Pretreatment
Sugar Tech, 2018Co-Authors: Arman Pazuki, Fatemeh Aflaki, Ali Ergül, Ekrem Gürel, Songül GürelAbstract:For sugar beet ( Beta vulgaris ) breeding, producing homozygous lines through haploid and doubled haploid techniques are preferred over conventional and time-consuming methods. Doubled haploid sugar beet production necessitates inducing ovules to develop into haploid plants, referred to as gynogenesis. The protocol involves an interaction between cold pretreatment of six genotypes of sugar beet inflorescences at 4 °C for 1 week or more and 6-benzylaminopurine (BAP) concentrations (1 or 2 mg L^−1) to increase the response rate of haploid Embryo Induction. Compared with freshly cultured ovules (6.49%), cold pretreatment for 1 week almost doubled the mean of haploid plantlet Induction rate (11.3%), whereas pretreatment for more than 1 week was not as effective as the control. Addition of 2 mg L^−1 BAP to the culture medium nearly doubled the Induction rate of the cultured ovules (10.75%), followed by 1 mg L^−1 BAP (7.78%) in comparison with hormone-free medium (5.69%). The highest gynogenesis rate (37.8%) was achieved when ovules were cultured on medium containing 2 mg L^−1 BAP following 1-week cold pretreatment. This combination approximately tripled the mean total haploid Embryo Induction rate of all the genotypes to 16.3% in comparison with the control (5.74%). However, the addition of BAP resulted in vitrification proportionately. As a result, 2 mg L^−1 BAP decreased the normal plantlet emergence (NPE) to one-third (7.59%) while 1 mg L^−1 BAP had a moderate effect (NPE: 18.98%) in comparison with hormone-free treatment (NPE: 24.35%). The results indicate that the combination of cold pretreatment and BAP is very effective in inducing haploid plants from recalcitrant genotypes of sugar beet, but BAP can have both advantages and disadvantages.
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Production of doubled haploids in sugar beet ( Beta vulgaris ): an efficient method by a multivariate experiment
Plant Cell Tissue and Organ Culture (PCTOC), 2017Co-Authors: Arman Pazuki, Fatemeh Aflaki, Songül Gürel, Ali Ergül, Ekrem GürelAbstract:The present paper describes a detailed study of a highly efficient protocol to multiply the number of haploids in sugar beet production and subsequent chromosome doubling. The protocol involves an experiment investigating factorial interactions between cold pretreatment, seven genotypes of sugar beet, and kinetin to improve haploid Embryo Induction. In addition, the effects of color of ovules and flower bud position on haploid Embryo Induction were investigated. After subjecting the data to analysis of variance or Student’s t test (P < .05), the effect sizes of the independent variables were also estimated. Cold pretreatment was effective in stimulating the ovules. The haploid Embryo Induction rate for 1-week cold pretreated ovules (9.01%) was higher than that of freshly cultured ones (6.15%). In comparison with hormone-free medium (5.16%), the gynogenesis rate for the media supplemented with 0.05 or 0.5 mg L−l kinetin increased to 7.58 and 10.05%, respectively. The genotype responses were significantly different. Interactions of kinetin × cold pretreatment, genotype × hormonal treatment, genotype × cold pretreatment, and the three-way interaction were statistically significant. Moreover, the main effects of flower bud position, ovule color, and comma-form ovule on gynogenic response were significant. After investigating the effect of 5 g L−l colchicine for 3, 5, or 7 min on one genotype’s (SG2) specimens, all the haploid plantlets from the other genotypes were treated for 5 min as the best treatment. The paper discusses interactions of the factors, which may be interesting for others aiming to breed doubled haploid sugar beet or possibly other related plant species.
Songül Gürel - One of the best experts on this subject based on the ideXlab platform.
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Gynogenesis Induction in Sugar Beet (Beta vulgaris) Improved by 6-Benzylaminopurine (BAP) and Synergized with Cold Pretreatment
Sugar Tech, 2018Co-Authors: Arman Pazuki, Fatemeh Aflaki, Ali Ergül, Ekrem Gürel, Songül GürelAbstract:For sugar beet ( Beta vulgaris ) breeding, producing homozygous lines through haploid and doubled haploid techniques are preferred over conventional and time-consuming methods. Doubled haploid sugar beet production necessitates inducing ovules to develop into haploid plants, referred to as gynogenesis. The protocol involves an interaction between cold pretreatment of six genotypes of sugar beet inflorescences at 4 °C for 1 week or more and 6-benzylaminopurine (BAP) concentrations (1 or 2 mg L^−1) to increase the response rate of haploid Embryo Induction. Compared with freshly cultured ovules (6.49%), cold pretreatment for 1 week almost doubled the mean of haploid plantlet Induction rate (11.3%), whereas pretreatment for more than 1 week was not as effective as the control. Addition of 2 mg L^−1 BAP to the culture medium nearly doubled the Induction rate of the cultured ovules (10.75%), followed by 1 mg L^−1 BAP (7.78%) in comparison with hormone-free medium (5.69%). The highest gynogenesis rate (37.8%) was achieved when ovules were cultured on medium containing 2 mg L^−1 BAP following 1-week cold pretreatment. This combination approximately tripled the mean total haploid Embryo Induction rate of all the genotypes to 16.3% in comparison with the control (5.74%). However, the addition of BAP resulted in vitrification proportionately. As a result, 2 mg L^−1 BAP decreased the normal plantlet emergence (NPE) to one-third (7.59%) while 1 mg L^−1 BAP had a moderate effect (NPE: 18.98%) in comparison with hormone-free treatment (NPE: 24.35%). The results indicate that the combination of cold pretreatment and BAP is very effective in inducing haploid plants from recalcitrant genotypes of sugar beet, but BAP can have both advantages and disadvantages.
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Production of doubled haploids in sugar beet ( Beta vulgaris ): an efficient method by a multivariate experiment
Plant Cell Tissue and Organ Culture (PCTOC), 2017Co-Authors: Arman Pazuki, Fatemeh Aflaki, Songül Gürel, Ali Ergül, Ekrem GürelAbstract:The present paper describes a detailed study of a highly efficient protocol to multiply the number of haploids in sugar beet production and subsequent chromosome doubling. The protocol involves an experiment investigating factorial interactions between cold pretreatment, seven genotypes of sugar beet, and kinetin to improve haploid Embryo Induction. In addition, the effects of color of ovules and flower bud position on haploid Embryo Induction were investigated. After subjecting the data to analysis of variance or Student’s t test (P < .05), the effect sizes of the independent variables were also estimated. Cold pretreatment was effective in stimulating the ovules. The haploid Embryo Induction rate for 1-week cold pretreated ovules (9.01%) was higher than that of freshly cultured ones (6.15%). In comparison with hormone-free medium (5.16%), the gynogenesis rate for the media supplemented with 0.05 or 0.5 mg L−l kinetin increased to 7.58 and 10.05%, respectively. The genotype responses were significantly different. Interactions of kinetin × cold pretreatment, genotype × hormonal treatment, genotype × cold pretreatment, and the three-way interaction were statistically significant. Moreover, the main effects of flower bud position, ovule color, and comma-form ovule on gynogenic response were significant. After investigating the effect of 5 g L−l colchicine for 3, 5, or 7 min on one genotype’s (SG2) specimens, all the haploid plantlets from the other genotypes were treated for 5 min as the best treatment. The paper discusses interactions of the factors, which may be interesting for others aiming to breed doubled haploid sugar beet or possibly other related plant species.
M. Höfer - One of the best experts on this subject based on the ideXlab platform.
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In vitro androgenesis in apple—improvement of the Induction phase
Plant Cell Reports, 2004Co-Authors: M. HöferAbstract:Based on a protocol for microspore culture in apple ( Malus domestica Borkh.), the Embryo Induction phase has been improved with regard to pretreatment of microspores for initiation of microspore Embryogenesis, the concentration of carbon source in the Induction medium and the microspore density in the suspension. Furthermore, the effect of the genotype was studied. To determine the efficiency of in vitro androgenesis, both methods, via anther and microspore culture, were investigated using the same bud material. A comparison of the efficiency of Embryo Induction in anther and microspore cultures showed that microspore culture resulted in an increase up to 10 times, depending on the genotype. The regeneration route in microspore culture is similar to that of androgenic Embryos via anther culture and showed adventitious shoot formation in most cases after a long period of secondary Embryogenesis.
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in vitro androgenesis in apple improvement of the Induction phase
Plant Cell Reports, 2004Co-Authors: M. HöferAbstract:Based on a protocol for microspore culture in apple (Malus domestica Borkh.), the Embryo Induction phase has been improved with regard to pretreatment of microspores for initiation of microspore Embryogenesis, the concentration of carbon source in the Induction medium and the microspore density in the suspension. Furthermore, the effect of the genotype was studied. To determine the efficiency of in vitro androgenesis, both methods, via anther and microspore culture, were investigated using the same bud material. A comparison of the efficiency of Embryo Induction in anther and microspore cultures showed that microspore culture resulted in an increase up to 10 times, depending on the genotype. The regeneration route in microspore culture is similar to that of androgenic Embryos via anther culture and showed adventitious shoot formation in most cases after a long period of secondary Embryogenesis.