The Experts below are selected from a list of 20151 Experts worldwide ranked by ideXlab platform
Michelle A. Digman - One of the best experts on this subject based on the ideXlab platform.
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Label-free assessment of pre-implantation Embryo Quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach
Scientific Reports, 2019Co-Authors: Ning Ma, Nabora Reyes De Mochel, Paula Duyen Pham, Michelle A. DigmanAbstract:Development of quantitative, safe and rapid techniques for assessing Embryo Quality provides significant advances in Assisted Reproductive Technologies (ART). Instead of assessing the Embryo Quality by the standard morphologic evaluation, we apply the phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) method to capture endogenous fluorescent biomarkers of pre-implantation Embryos as a non-morphological caliber for Embryo Quality. Here, we identify, under hypoxic and non-hypoxic conditions, the unique spectroscopic trajectories at different stages of mouse pre-implantation development, which is referred to as the developmental, or “D-trajectory”, that consists of fluorescence lifetime from different stages of mouse pre-implantation Embryos. The D-trajectory correlates with intrinsic fluorescent species from a distinctive energy metabolism and oxidized lipids, as seen with Third Harmonic Generation (THG) that changes over time. In addition, we have defined a non-morphological Embryo Viability Index (EVI) to distinguish pre-implantation Embryo Quality using the Distance Analysis (DA), a machine learning algorithm to process the fluorescence lifetime distribution patterns. We show, under our experimental conditions, that the phasor-FLIM approach provides a much-needed non-invasive quantitative technology for identifying healthy Embryos at the early compaction stage with 86% accuracy. The DA and phasor-FLIM method may provide the opportunity to improve implantation success rates for in vitro fertilization clinics.
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label free assessment of pre implantation Embryo Quality by the fluorescence lifetime imaging microscopy flim phasor approach
bioRxiv, 2018Co-Authors: Nabora Reyes De Mochel, Paula Duyen Anh Pham, Tae Yeon Yoo, Ken W Y Cho, Michelle A. DigmanAbstract:Development of quantitative, safe and rapid techniques for assessing Embryo Quality provides significant advances in Assisted Reproductive Technologies (ART). We apply the phasor-FLIM method to capture endogenous fluorescent biomarkers of pre-implantation Embryos as a non-morphological caliber for Embryo Quality. Here, we identify the developmental, or 9D-trajectory9, that consists of fluorescence lifetime from different stages of mouse pre-implantation Embryos. The D-trajectory correlates with intrinsic fluorescent species from a distinctive energy metabolism and oxidized lipids, as seen with Third Harmonic Generation (THG) that changes over time. In addition, we have defined an Embryo Viability Index (EVI) to distinguish pre-implantation Embryo Quality using the Distance Analysis, a machine learning algorithm to process the fluorescence lifetime distribution patterns. We show that the phasor-FLIM approach provides a much-needed non-invasive quantitative technology for identifying healthy Embryos at the early compaction stage with 86% accuracy. This may increase Embryo implantation success for in vitro fertilization clinics.
J E P Santos - One of the best experts on this subject based on the ideXlab platform.
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reduced progesterone concentration during growth of the first follicular wave affects Embryo Quality but has no effect on Embryo survival post transfer in lactating dairy cows
Reproduction, 2011Co-Authors: F Rivera, J E P Santos, L G D Mendonca, G Lopes, Rolando V Perez, Marcel Amstalden, Abelardo Correacalderon, R C ChebelAbstract:Fertility of lactating dairy cows is associated with reduced progesterone (P(4)) concentration compared with nonlactating animals. The objective of the current study was to determine whether P(4) during growth of the first follicular wave (FFW) affects Embryo Quality. Lactating Holstein cows at 33±3 days post partum were allocated to one of three treatments. Cows in the FFW and FFW with P(4) (FFWP) treatments started the superstimulation protocol on day 1 of the estrous cycle and second follicular wave (SFW) cows started the superstimulation protocol on estrous cycle day 7. Cows were superstimulated with 400 mg of NIH-FSH-P1 (FSH) given twice daily for 5 days, two prostaglandin F(2α) (PGF(2α)) injections given with the ninth and tenth injections of FSH, GNRH given 48 h after the first PGF(2α) injection, and timed insemination 12 and 24 h after the GNRH injection. Cows in the FFWP treatment received two intravaginal P(4) inserts during the superstimulation. Embryos were recovered 6.5 days after artificial insemination and excellent/good and fair Embryos were frozen and transferred. Blood was sampled daily from estrous cycle day 0 until insemination from donor cows. During the superstimulation protocol, P(4) was (P<0.01) greatest for SFW cows followed by FFWP and FFW cows respectively. The percentage of Embryos-oocytes from SFW and FFWP cows classified as excellent/good and fair Embryos was (P=0.02) greater than those of FFW cows. Pregnancy per Embryo transfer was not (P≥0.73) affected by Embryo donor treatment. Reduced Embryo Quality of cows induced to ovulate the follicles from the first follicular wave is a consequence of reduced P(4) during follicle growth.
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period of dominance of the ovulatory follicle influences Embryo Quality in lactating dairy cows
Reproduction, 2009Co-Authors: R L A Cerri, Heloisa M Rutigliano, R C Chebel, J E P SantosAbstract:Length of dominance of the ovulatory follicle and exposure to oestradiol (OE(2)) during proestrus can affect fertility. Lactating cows had their oestrous cycle pre-synchronized and were subjected to one of the four synchronization treatments. Cows in the oestrus detection (OD) treatment received GnRH on day 6 of the oestrous cycle, PGF(2alpha) 7 days later, and were inseminated at detected oestrus. The remaining cows were subjected to the Ovsynch (OVS) protocol (day 0 GnRH, day 7 PGF(2alpha), day 9 GnRH, and timed artificial insemination (AI) 12 h later) starting on day 3 (OVS3) or day 6 (OVS6 and OVS6E) of the oestrous cycle. Cows in the OVS6E treatment received an injection of 0.5 mg oestradiol cypionate 36 h before AI. Ovaries were examined by ultrasonography and blood was sampled for progesterone and OE(2) concentrations. Uteri were flushed 6 days after AI and recovered Embryos-oocytes evaluated. Diameter of the ovulatory follicle at AI differed (P 7. Proportions of viable Embryos in relation to Embryos and Embryos-oocytes recovered were smaller for OVS3 cows (P<0.01) than the other treatments, and Embryos from OVS3 cows also had fewer (P<0.01) blastomeres and tended (P=0.09) to have a lower proportion of live blastomeres. Extending the period of follicle dominance did not alter fertilization but reduced (P<0.001) Embryo Quality. Embryo Quality was compromised even when the dominance of the ovulatory follicle was extended by only 1.5 days.
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effect of fat source differing in fatty acid profile on metabolic parameters fertilization and Embryo Quality in high producing dairy cows
Journal of Dairy Science, 2009Co-Authors: R L A Cerri, Heloisa M Rutigliano, R C Chebel, J E P Santos, S O Juchem, R G S Bruno, K N Galvao, W W ThatcherAbstract:The objectives were to evaluate the effects of source of fatty acids (FA) on Embryo Quality of dairy cows. A total of 154 Holstein cows were assigned randomly to 1 of 2 sources of FA supplemented at 2% of the dietary dry matter as calcium salts of either palm oil (PO) or linoleic and trans-octadecenoic acids (LTFA) from 25 d prepartum to 80 d in milk (DIM). Cows were presynchronized beginning at 30 +/- 3 DIM and then subjected to the Ovsynch protocol beginning on d 39 +/- 3 postpartum. Timed artificial insemination was performed 12 h after the final GnRH of the Ovsynch protocol with semen from a single sire of proven fertility. The uteri of cows were nonsurgically flushed at 5 d after artificial insemination for collection of Embryos-oocytes. Ovaries were examined by ultrasonography throughout the synchronization protocol. Blood was sampled and plasma was analyzed for concentrations of metabolites and hormones. The body condition score and yields of milk and milk components were measured throughout the first 90 DIM. Treatment did not affect concentrations of nonesterified FA, beta-hydroxybutyrate, glucose, and progesterone in plasma. Body condition was similar between treatments. Milk production was similar between treatments, but concentrations of fat in milk and yields of fat and 3.5% fat-corrected milk decreased in cows fed LTFA, whereas concentration of true protein increased. Source of dietary FA did not influence ovulatory responses, diameter of the ovulatory follicle, and diameter of the corpus luteum during synchronization. Embryo-oocyte recovery relative to the number of corpora lutea did not differ between treatments. Fertilization tended to increase in cows fed LTFA compared with cows fed PO. Feeding LTFA improved the proportion of excellent-, good-, and fair-Quality Embryos, and Embryos from cows fed LTFA had a greater number of blastomeres than Embryos from cows fed PO. Feeding a more unsaturated source of FA improved fertilization and Embryo development in lactating dairy cows, despite similar indicators of metabolic status.
Joop S E Laven - One of the best experts on this subject based on the ideXlab platform.
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preconceptional maternal vegetable intake and paternal smoking are associated with pre implantation Embryo Quality
Reproductive Sciences, 2020Co-Authors: Jeffrey Hoek, Eric A P Steegers, Sam Schoenmakers, Esther B Baart, Maria P H Koster, Sten P Willemsen, Eva S Van Marion, Joop S E Laven, Regine P M SteegerstheunissenAbstract:Inadequate nutrition and lifestyle behaviors, particularly during the periconception period, are associated with a negative impact on Embryonic and subsequent fetal development. We investigated the associations between parental nutritional and lifestyle factors and pre-implantation Embryo development. A total of 113 women and 41 partners, with a corresponding 490 Embryos, who underwent intracytoplasmic sperm injection (ICSI) treatment subscribed to the mHealth coaching platform “Smarter Pregnancy.” At baseline, nutrition and lifestyle behaviors (intake of fruits, vegetables, folic acid, and smoking and alcohol use) were identified and risk scores were calculated. A lower risk score represents healthier behavior. As outcome measure, a time-lapse morphokinetic selection algorithm (KIDScore) was used to rank pre-implantation Embryo Quality on a scale from 1 (poor) to 5 (good) after being cultured in the Embryoscope™ time-lapse incubator until Embryonic day 3. To study the association between the nutritional and lifestyle risk scores and the KIDScore in men and women, we used a proportional odds model. In women, the dietary risk score (DRS), a combination of the risk score of fruits, vegetables, and folic acid, was negatively associated with the KIDScore (OR 0.86 (95% CI 0.76 to 0.98), p = 0.02). This could mainly be attributed to an inadequate vegetable intake (OR 0.76 (95% CI 0.59 to 0.96), p = 0.02). In men, smoking was negatively associated with the KIDscore (OR 0.53 (95% CI 0.33 to 0.85), p < 0.01). We conclude that inadequate periconceptional maternal vegetable intake and paternal smoking significantly reduce the implantation potential of Embryos after ICSI treatment. Identifying modifiable lifestyle risk factors can contribute to directed, personalized, and individual recommendations that can potentially increase the chance of a healthy pregnancy.
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Preconceptional Maternal Vegetable Intake and Paternal Smoking Are Associated with Pre-implantation Embryo Quality
Reproductive Sciences, 2020Co-Authors: Jeffrey Hoek, Eric A P Steegers, Sam Schoenmakers, Esther B Baart, Maria P H Koster, Sten P Willemsen, Joop S E Laven, Eva S. Marion, Régine P. M. Steegers-theunissenAbstract:Inadequate nutrition and lifestyle behaviors, particularly during the periconception period, are associated with a negative impact on Embryonic and subsequent fetal development. We investigated the associations between parental nutritional and lifestyle factors and pre-implantation Embryo development. A total of 113 women and 41 partners, with a corresponding 490 Embryos, who underwent intracytoplasmic sperm injection (ICSI) treatment subscribed to the mHealth coaching platform “Smarter Pregnancy.” At baseline, nutrition and lifestyle behaviors (intake of fruits, vegetables, folic acid, and smoking and alcohol use) were identified and risk scores were calculated. A lower risk score represents healthier behavior. As outcome measure, a time-lapse morphokinetic selection algorithm (KIDScore) was used to rank pre-implantation Embryo Quality on a scale from 1 (poor) to 5 (good) after being cultured in the Embryoscope™ time-lapse incubator until Embryonic day 3. To study the association between the nutritional and lifestyle risk scores and the KIDScore in men and women, we used a proportional odds model. In women, the dietary risk score (DRS), a combination of the risk score of fruits, vegetables, and folic acid, was negatively associated with the KIDScore (OR 0.86 (95% CI 0.76 to 0.98), p = 0.02). This could mainly be attributed to an inadequate vegetable intake (OR 0.76 (95% CI 0.59 to 0.96), p = 0.02). In men, smoking was negatively associated with the KIDscore (OR 0.53 (95% CI 0.33 to 0.85), p
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anti mullerian hormone a marker for oocyte quantity oocyte Quality and Embryo Quality
Reproductive Biomedicine Online, 2008Co-Authors: Lie S Fong, Esther B Baart, Elena Martini, Izaak Schipper, Jenny A Visser, A P N Themmen, F H De Jong, B C Fauser, Joop S E LavenAbstract:Serum anti-Mullerian hormone (AMH) concentrations decline with increasing age and constitute a sensitive marker for ovarian ageing. In addition, basal serum AMH concentrations predict ovarian response during IVF cycles. Concomitantly, oocyte quantity and Embryo Quality decrease with advancing age. Hence, it was postulated that AMH in serum constitutes a marker for Embryo Quality. Women aged 37 years and younger with regular menstrual cycles, normal body mass index and partners with normal semen parameters were randomly assigned to either a standard or mild stimulation protocol for IVF treatment. Blood samples were drawn at cycle day 3 and at the day of human chorionic gonadotrophin administration. Embryo Quality was assessed using Embryo morphology score and preimplantation genetic screening. Serum AMH concentrations on cycle day 3 were correlated with the number of oocytes retrieved in both groups. AMH and Embryo morphology were correlated after mild stimulation, but not after conventional ovarian stimulation. AMH and the chromosomal competence of Embryos were not correlated. Serum AMH is predictive for ovarian response to stimulation. However, the lack of a consistent correlation with Embryo morphology and Embryo aneuploidy rate is not in favour of a direct relationship between oocyte quantity and Embryo Quality.
Marcos Meseguer - One of the best experts on this subject based on the ideXlab platform.
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effect of oocyte vitrification on Embryo Quality time lapse analysis and morphokinetic evaluation
Fertility and Sterility, 2017Co-Authors: Ana Cobo, A Coello, J Remohi, J J Serrano, Jose Maria De Los Santos, Marcos MeseguerAbstract:Objective To analyze whether oocyte vitrification may affect subsequent Embryo development from a morphokinetic standpoint by means of time-lapse imaging. Design Observational cohort study. Setting University-affiliated private IVF center. Patient(s) Ovum donation cycles conducted with the use of vitrified (n = 631 cycles; n=3,794 Embryos) or fresh oocytes (n = 1,359 cycles; n=9,935 Embryos) over 2 years. Interventions(s) None. Main Outcome Measure(s) Embryo development was analyzed in a time-lapse imaging incubator. The studied variables included time to 2 cells (t2), 3 cells (t3), 4 cells (t4), 5 cells (t5), morula (tM), and cavitated, early, and hatching blastocyst (tB, tEB, tHB) as well as 2nd cell cycle duration (cc2 = t3 − t2). All of the Embryos were classified according to the hierarchic tree model currently used for Embryo selection. The analyzed variables were compared with the use of analysis of variance or chi-square and included 95% confidence intervals (CIs). Result(s) The Embryos that originated from vitrified oocytes showed a delay of ∼1 hour from the first division to 2 cells (t2) to the time of blastulation (tB). The Embryos that originated from vitrified oocytes showed a delay of ∼1 hour from the 1st division to 2 cells (t2) to the time of blastulation (tB) ( P Conclusion(s) The Embryo Quality of vitrified oocytes was not impaired: cc2, Quality according to our hierarchic morphokinetic model, and implantation rates were similar between fresh and vitrified oocytes. However, morphokinetic differences were observed from t2 to tB. Our main study limitation was the retrospective nature of the analysis, although a large database was studied.
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Embryo Quality blastocyst and ongoing pregnancy rates in oocyte donation patients whose Embryos were monitored by time lapse imaging
Journal of Assisted Reproduction and Genetics, 2011Co-Authors: Maria Cruz, Blanca Gadea, Nicolas Garrido, Mar Martinez, Inma Perezcano, Manuel Munoz, Kamilla Soe Pedersen, Marcos MeseguerAbstract:Purpose In the current study, our aim was to demonstrate that EmbryoScope incubation conditions is comparable to standard laboratory incubation circumstances by comparing Embryo Quality, development and ongoing pregnancy rates between the EmbryoScope (ES) and a standard incubator (SI). We analyzed 478 Embryos from 60 couples undergoing oocyte donation were included in the study.
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Embryo Quality blastocyst and ongoing pregnancy rates in oocyte donation patients whose Embryos were monitored by time lapse imaging
Journal of Assisted Reproduction and Genetics, 2011Co-Authors: Maria Jose Santa Cruz, Blanca Gadea, Nicolas Garrido, Kamilla Sofie Pedersen, Mar Martinez, Inma Perezcano, Manuel Munoz, Marcos MeseguerAbstract:Purpose In the current study, our aim was to demonstrate that EmbryoScope incubation conditions is comparable to standard laboratory incubation circumstances by comparing Embryo Quality, development and ongoing pregnancy rates between the EmbryoScope (ES) and a standard incubator (SI). We analyzed 478 Embryos from 60 couples undergoing oocyte donation were included in the study.
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female obesity impairs in vitro fertilization outcome without affecting Embryo Quality
Fertility and Sterility, 2010Co-Authors: Jose Bellver, Jose Remohi, Yanira Ayllon, Marcos Ferrando, M A B Melo, Eduardo Goyri, Antonio Pellicer, Marcos MeseguerAbstract:Objective To compare Embryo Quality and reproductive outcome in our IVF program according to the women's body mass index (BMI). Design Retrospective study. Setting University-affiliated infertility clinic, between January 2001 and April 2007. Patient(s) Women undergoing a total of 6,500 IVF–intracytoplasmic sperm injection (ICSI) cycles. Intervention(s) Six thousand five hundred IVF-ICSI cycles were included and divided into four groups: lean ( 2 ; n=1,070; 16.5%); normal (20–24.9 kg/m 2 ; n=3,930; 60.5%); overweight (25–29.9 kg/m 2 ; n=1,081; 16.6%); and obese (≥30 kg/m 2 ; n=419; 6.4%). Main Outcome Measure(s) Comparison of Embryo Quality and reproductive outcome (implantation, pregnancy, miscarriage, and live birth rates) among BMI groups. Result(s) No difference in insemination procedure, fertilization rate, day of ET, mean number of transferred and cryopreserved Embryos, percentage of blastocyst transfers, or Embryo Quality on day 2 and 3 was found among groups. However, implantation, pregnancy, and live birth rates were poorer in obese women. In fact, pregnancy and live birth rates were reduced progressively with each unit of BMI (kilograms per square meter) with a significant odds ratio of 0.984 (95% confidence interval 0.972–0.997) and 0.981 (95% confidence interval 0.967–0.995), respectively. In addition, the cumulative pregnancy rate after four IVF cycles was reduced as BMI increased. Conclusion(s) Female obesity impairs IVF outcome, but Embryo Quality is not affected, pointing to an alteration in the uterine environment.
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antral follicle count afc can be used in the prediction of ovarian response but cannot predict the oocyte Embryo Quality or the in vitro fertilization outcome in an egg donation program
Fertility and Sterility, 2009Co-Authors: M A B Melo, Jose Bellver, Antonio Pellicer, Marcos Meseguer, Nicolas Garrido, Claudio Alvarez, Jose RemohiAbstract:Objective To verify whether the antral follicle count (AFC) could predict ovarian response, oocyte/Embryo Quality, and IVF outcome. Design Prospective study. Setting Instituto Universitario–InstitutoValenciano de Infertilidad, Valencia, Spain. Patient(s) One thousand seventy-four donors and 975 oocyte recipient cycles. Intervention(s) Controlled ovarian hyperstimulation (COH), endometrial preparation, IVF/intracytoplasmic sperm injection, ET. Main Outcome Measure(s) COH and oocyte/Embryo Quality parameters and IVF outcome. Result(s) We observed lower E 2 levels and fewer mature retrieved oocyte numbers among donors who showed an AFC that was Conclusion(s) AFC is a noninvasive and simple tool that can improve the oocyte donor's selection of an egg donation program. This study suggests that AFC is a good predictor of ovarian response but cannot be used to predict oocyte/Embryo Quality or IVF outcome.
Ning Ma - One of the best experts on this subject based on the ideXlab platform.
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Label-free assessment of pre-implantation Embryo Quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach
Scientific Reports, 2019Co-Authors: Ning Ma, Nabora Reyes De Mochel, Paula Duyen Pham, Michelle A. DigmanAbstract:Development of quantitative, safe and rapid techniques for assessing Embryo Quality provides significant advances in Assisted Reproductive Technologies (ART). Instead of assessing the Embryo Quality by the standard morphologic evaluation, we apply the phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) method to capture endogenous fluorescent biomarkers of pre-implantation Embryos as a non-morphological caliber for Embryo Quality. Here, we identify, under hypoxic and non-hypoxic conditions, the unique spectroscopic trajectories at different stages of mouse pre-implantation development, which is referred to as the developmental, or “D-trajectory”, that consists of fluorescence lifetime from different stages of mouse pre-implantation Embryos. The D-trajectory correlates with intrinsic fluorescent species from a distinctive energy metabolism and oxidized lipids, as seen with Third Harmonic Generation (THG) that changes over time. In addition, we have defined a non-morphological Embryo Viability Index (EVI) to distinguish pre-implantation Embryo Quality using the Distance Analysis (DA), a machine learning algorithm to process the fluorescence lifetime distribution patterns. We show, under our experimental conditions, that the phasor-FLIM approach provides a much-needed non-invasive quantitative technology for identifying healthy Embryos at the early compaction stage with 86% accuracy. The DA and phasor-FLIM method may provide the opportunity to improve implantation success rates for in vitro fertilization clinics.