The Experts below are selected from a list of 2526 Experts worldwide ranked by ideXlab platform

David L Cochran - One of the best experts on this subject based on the ideXlab platform.

  • Enamel Matrix Derivative protein components and osteoinductive properties
    Journal of Periodontology, 2014
    Co-Authors: Bradshaw M Stout, David L Cochran, Brian J Alent, Peter Pedalino, Ryan Holbrook, Jelica Gluhakheinrich, Yong Cui, Marie A Harris, Anja C Gemperli, David E Deas
    Abstract:

    Background: Although Enamel Matrix Derivative (EMD) has demonstrated the ability to promote angiogenesis and osteogenesis both in vitro and in vivo, the specific elements within the EMD compound responsible for these effects remain unknown.Methods: Nine different protein pools from a commercially produced EMD were collected based on molecular weight. Six of these pools, along with the complete EMD unfractionated compound and positive and negative controls, were tested for their ability to induce bone formation in a calvarial induction assay. Immunocytochemistry of phosphorylated SMAD1/5/8 (phospho-SMAD), osterix, and vascular endothelial growth factor A (VEGF-A) was carried out at selected time points. Finally, proteomic analysis was completed to determine the specific protein-peptide content of the various osteoinductive pools.Results: One of the lower-molecular-weight pools tested, pool 7, showed bone induction responses significantly greater than those of the other pools and the complete EMD compound a...

  • In vivo angiogenic activity of Enamel Matrix Derivative.
    Journal of periodontology, 2010
    Co-Authors: Amy S. Kauvar, David L Carnes, Daniel S. Thoma, David L Cochran
    Abstract:

    Background: Porcine Enamel Matrix Derivative (EMD) has a clinical use in facilitating periodontal healing by enhancing the regeneration of alveolar bone, cementum, and periodontal ligament. The mechanism of clinical use has not been elucidated, but in vitro studies suggest that EMD may enhance healing, in part, by stimulating angiogenesis. This study analyzes the effect of EMD on the production of blood vessels in the chorioallantoic membrane (CAM) of the developing chicken egg.Methods: Various amounts of EMD ranging from 15 to 125 ng/5 μl were pipetted onto a 3-mm diameter × 2-mm thick bioabsorbable hemostatic gelatin and placed onto the surface of the CAM. Recombinant human amelogenin and a purified 5-kDa protein fraction derived from EMD were also tested at various amounts ranging from 15 to 62 ng/5 μl. A mixture of fibroblast growth factor and vascular endothelial growth factor served as the positive control. The negative control was 0.9% saline. A histologic examination of the interface of the gelati...

  • Purification and analysis of a 5 kDa component of Enamel Matrix Derivative
    Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2007
    Co-Authors: Alexandra Mumulidu, David L Cochran, Michel Dard, Bergisa Hildebrand, Beata Fabi, Lars Hammarström, Stephanie Lemoult
    Abstract:

    High performance liquid chromatography (HPLC) methods were used to analyse a 5kDa component purified from Enamel Matrix Derivative (EMD), the active ingredient in Emdogain, a commercial product for periodontal tissue regeneration. After initial purification by size-exclusion chromatography (SEC) on a 100 cm x 5 cm column (Bio-Gel P-30 Fine, 280 nm), collected fractions were analysed by size-exclusion HPLC (SE HPLC; TSK-Gel Super SW2000, 220 nm). The fractions containing only the 5kDa component were analysed by reversed-phase high-pressure chromatography (RP HPLC; YMC-Pack ODS-A, 200 nm), revealing four peaks of the 5kDa component. From 1200 mg of EMD (of which 9% is the 5kDa component), approximately 65 mg of lyophilised 5kDa component were obtained, corresponding to a recovery of 60%. The SE HPLC method was mainly suitable for qualitative analysis, whereas the RP HPLC method was appropriate for both qualitative and quantitative analysis.

  • In Vitro Effects of Enamel Matrix Derivative on Microvascular Cells
    Journal of periodontology, 2007
    Co-Authors: Scott R. Schlueter, David L Carnes, David L Cochran
    Abstract:

    Background: Periodontal regeneration requires a coordinated series of events that includes not only the recruitment of periodontal ligament (PDL)-specific cells, but vascular cells as well. The mechanisms of action of Enamel Matrix Derivative (EMD) are poorly understood, and its effects on vascular cells are unknown. The objective of this study was to examine the extent to which EMD affects angiogenesis and PDL cell recruitment.Methods: The effects of EMD on human microvascular endothelial cells (HMVECs) were determined by examining proliferation, chemotaxis, angiogenesis, and migration. Proliferation was determined using water-soluble tetrazolium salt (WST)-1 reagent. Chemotaxis was determined using microporous-culture well inserts. Angiogenesis was assessed on plates containing matrigel. The effects of HMVECs on the migration of PDL cells were assessed by evaluating PDL cell outgrowth from collagen gels cultured in the presence of HMVECs on fibrin Matrix and surrounded by fibronectin-containing fibrin c...

  • Evaluation of Enamel Matrix Derivative as an adjunct to non‐surgical periodontal therapy
    Journal of clinical periodontology, 2003
    Co-Authors: Mauricio A. Gutierrez, James T. Mellonig, David L Cochran
    Abstract:

    Objectives: The aim of this study was to evaluate the adjunctive use of Enamel Matrix Derivative (EMD) on periodontal healing following nonsurgical periodontal therapy (scaling and root planing – SRP). Material and methods: The study was performed as an intraindividual, longitudinal trial of 3 months duration with a double-blinded, split-mouth, controlled, and randomized design. Twenty-two patients with moderate to severe chronic periodontitis were enrolled in the study. In each patient, two sites with pocket depths 5 mm and with radiographic angular bone defects >3 mm were selected. Baseline examination included measurement of probing pocket depth (PPD) and clinical attachment levels (CAL). The presence or absence of plaque and bleeding on probing at selected sites was also recorded. Following initial examination, full-mouth SRP was performed. Study sites were then treated with 24% EDTA for 2 min, followed by thorough irrigation with sterile saline. The sites were then randomized. The experimental site received subgingival application of Enamel Matrix Derivative (Emdogain®, BIORA AB, Malmo, Sweden). The control site received no additional treatment. At 3 months, all sites were re-examined. The response to therapy in experimental and control sites was evaluated, using change in probing depth and CAL as the primary outcome variables. Statistical analysis (paired t-tests) was used to compare response to treatment in control versus experimental sites. Results: Statistically significant changes in PPD and CAL were seen in both treatment groups from baseline to 3 months. The mean PPD reduction was 2.3±0.5 mm for control sites and 2.0±0.3 mm for experimental sites. The mean CAL gain was 1.8±0.4 mm for control sites, and 1.4±0.3 mm for experimental sites. Statistical analysis, however, revealed no significant difference in PPD reduction or CAL gain between experimental and control groups (p>0.4). In addition, no difference was found between treatment groups in bleeding or plaque indices at 3 months. Conclusion: The findings from the present study do not support the use of EMD during routine, nonsurgical debridement of periodontal pockets as measured 3 months post SRP.

Anton Sculean - One of the best experts on this subject based on the ideXlab platform.

  • split mouth evaluation of connective tissue graft with or without Enamel Matrix Derivative for the treatment of isolated gingival recession defects in dogs
    Clinical Oral Investigations, 2019
    Co-Authors: Yoshinori Shirakata, Anton Sculean, Toshiaki Nakamura, Yukiya Shinohara, K Nakamurahasegawa, Chikoto Hashiguchi, Naoshi Takeuchi, T Imafuji, Kazuyuki Noguchi
    Abstract:

    Objectives The potential additive effect of an Enamel Matrix Derivative (EMD) to a subepithelial connective tissue graft (CTG) for recession coverage is still controversially discussed. Therefore, the aim of this study was to histologically evaluate the healing of gingival recessions treated with coronally advanced flap (CAF) and CTG with or without EMD in dogs.

  • Combination of Collagen Barrier Membrane with Enamel Matrix Derivative-Liquid Improves Osteoblast Adhesion and Differentiation.
    The International journal of oral & maxillofacial implants, 2017
    Co-Authors: Richard J Miron, Masako Fujioka-kobayashi, Dieter D. Bosshardt, Daniel Buser, Yufeng Zhang, Anton Sculean
    Abstract:

    PURPOSE: Collagen barrier membranes were first introduced to regenerative periodontal and oral surgery to prevent fast ingrowing soft tissues (ie, epithelium and connective tissue) into the defect space. More recent attempts have aimed at combining collagen membranes with various biologics/growth factors to speed up the healing process and improve the quality of regenerated tissues. Recently, a new formulation of Enamel Matrix Derivative in a liquid carrier system (Osteogain) has demonstrated improved physico-chemical properties for the adsorption of Enamel Matrix Derivative to facilitate protein adsorption to biomaterials. The aim of this pioneering study was to investigate the use of Enamel Matrix Derivative in a liquid carrier system in combination with collagen barrier membranes for its ability to promote osteoblast cell behavior in vitro. MATERIALS AND METHODS: Undifferentiated mouse ST2 stromal bone marrow cells were seeded onto porcine-derived collagen membranes alone (control) or porcine membranes + Enamel Matrix Derivative in a liquid carrier system. Control and Enamel Matrix Derivative-coated membranes were compared for cell recruitment and cell adhesion at 8 hours; cell proliferation at 1, 3, and 5 days; and real-time polymerase chain reaction (PCR) at 3 and 14 days for genes encoding Runx2, collagen1alpha2, alkaline phosphatase, and bone sialoprotein. Furthermore, alizarin red staining was used to investigate mineralization. RESULTS: A significant increase in cell adhesion was observed at 8 hours for barrier membranes coated with Enamel Matrix Derivative in a liquid carrier system, whereas no significant difference could be observed for cell proliferation or cell recruitment. Enamel Matrix Derivative in a liquid carrier system significantly increased alkaline phosphatase mRNA levels 2.5-fold and collagen1alpha2 levels 1.7-fold at 3 days, as well as bone sialoprotein levels twofold at 14 days postseeding. Furthermore, collagen membranes coated with Enamel Matrix Derivative in a liquid carrier system demonstrated a sixfold increase in alizarin red staining at 14 days when compared with collagen membrane alone. CONCLUSION: The combination of Enamel Matrix Derivative in a liquid carrier system with a barrier membrane significantly increased cell attachment, differentiation, and mineralization of osteoblasts in vitro. Future animal testing is required to fully characterize the additional benefits of combining Enamel Matrix Derivative in a liquid carrier system with a barrier membrane for guided bone or tissue regeneration.

  • Healing of localized gingival recessions treated with a coronally advanced flap alone or combined with an Enamel Matrix Derivative and a porcine acellular dermal Matrix: a preclinical study.
    Clinical oral investigations, 2015
    Co-Authors: Yoshinori Shirakata, Dieter D. Bosshardt, Anton Sculean, Yukiya Shinohara, Naoshi Takeuchi, Kotaro Sena, Kazuyuki Noguchi
    Abstract:

    Objective This study aimed to evaluate the effects of a porcine acellular dermal Matrix (PADM) with or without an Enamel Matrix Derivative (EMD) on gingival recession defects treated with a coronally advanced flap (CAF) in dogs.

  • Enamel Matrix Derivative and bone grafts for periodontal regeneration of intrabony defects. A systematic review and meta-analysis
    Clinical oral investigations, 2015
    Co-Authors: M. Matarasso, Vincenzo Iorio-siciliano, Andrea Blasi, Luca Ramaglia, Giovanni E. Salvi, Anton Sculean
    Abstract:

    Objective The aim of the present systematic review and meta-analysis was to assess the clinical efficacy of regenerative periodontal surgery of intrabony defects using a combination of Enamel Matrix Derivative (EMD) and bone graft compared with that of EMD alone.

  • Comparison of the Capacity of Enamel Matrix Derivative Gel and Enamel Matrix Derivative in Liquid Formulation to Adsorb to Bone Grafting Materials
    Journal of periodontology, 2015
    Co-Authors: Richard J Miron, Dieter D. Bosshardt, Daniel Buser, Yufeng Zhang, Anja C Gemperli, Michel Dard, Stefano Tugulu, Oana M. Caluseru, Fatiha Chandad, Anton Sculean
    Abstract:

    Background: The use of an Enamel Matrix Derivative (EMD) has been shown to enhance periodontal regeneration (e.g., formation of root cementum, periodontal ligament, and alveolar bone). However, in certain clinical situations, the use of EMD alone may not be sufficient to prevent flap collapse or provide sufficient stability of the blood clot. Data from clinical and preclinical studies have demonstrated controversial results after application of EMD combined with different types of bone grafting materials in periodontal regenerative procedures. The aim of the present study is to investigate the adsorption properties of Enamel Matrix proteins to bone grafts after surface coating with either EMD (as a liquid formulation) or EMD (as a gel formulation).Methods: Three different types of grafting materials, including a natural bone mineral (NBM), demineralized freeze-dried bone allograft (DFDBA), or a calcium phosphate (CaP), were coated with either EMD liquid or EMD gel. Samples were analyzed by scanning electr...

Richard J Miron - One of the best experts on this subject based on the ideXlab platform.

  • Combination of Collagen Barrier Membrane with Enamel Matrix Derivative-Liquid Improves Osteoblast Adhesion and Differentiation.
    The International journal of oral & maxillofacial implants, 2017
    Co-Authors: Richard J Miron, Masako Fujioka-kobayashi, Dieter D. Bosshardt, Daniel Buser, Yufeng Zhang, Anton Sculean
    Abstract:

    PURPOSE: Collagen barrier membranes were first introduced to regenerative periodontal and oral surgery to prevent fast ingrowing soft tissues (ie, epithelium and connective tissue) into the defect space. More recent attempts have aimed at combining collagen membranes with various biologics/growth factors to speed up the healing process and improve the quality of regenerated tissues. Recently, a new formulation of Enamel Matrix Derivative in a liquid carrier system (Osteogain) has demonstrated improved physico-chemical properties for the adsorption of Enamel Matrix Derivative to facilitate protein adsorption to biomaterials. The aim of this pioneering study was to investigate the use of Enamel Matrix Derivative in a liquid carrier system in combination with collagen barrier membranes for its ability to promote osteoblast cell behavior in vitro. MATERIALS AND METHODS: Undifferentiated mouse ST2 stromal bone marrow cells were seeded onto porcine-derived collagen membranes alone (control) or porcine membranes + Enamel Matrix Derivative in a liquid carrier system. Control and Enamel Matrix Derivative-coated membranes were compared for cell recruitment and cell adhesion at 8 hours; cell proliferation at 1, 3, and 5 days; and real-time polymerase chain reaction (PCR) at 3 and 14 days for genes encoding Runx2, collagen1alpha2, alkaline phosphatase, and bone sialoprotein. Furthermore, alizarin red staining was used to investigate mineralization. RESULTS: A significant increase in cell adhesion was observed at 8 hours for barrier membranes coated with Enamel Matrix Derivative in a liquid carrier system, whereas no significant difference could be observed for cell proliferation or cell recruitment. Enamel Matrix Derivative in a liquid carrier system significantly increased alkaline phosphatase mRNA levels 2.5-fold and collagen1alpha2 levels 1.7-fold at 3 days, as well as bone sialoprotein levels twofold at 14 days postseeding. Furthermore, collagen membranes coated with Enamel Matrix Derivative in a liquid carrier system demonstrated a sixfold increase in alizarin red staining at 14 days when compared with collagen membrane alone. CONCLUSION: The combination of Enamel Matrix Derivative in a liquid carrier system with a barrier membrane significantly increased cell attachment, differentiation, and mineralization of osteoblasts in vitro. Future animal testing is required to fully characterize the additional benefits of combining Enamel Matrix Derivative in a liquid carrier system with a barrier membrane for guided bone or tissue regeneration.

  • Enamel Matrix Derivative inflammation and soft tissue wound healing
    Journal of Periodontal Research, 2015
    Co-Authors: Richard J Miron, Michel Dard, Miron Weinreb
    Abstract:

    Over 15 years have now passed since Enamel Matrix Derivative (EMD) emerged as an agent capable of periodontal regeneration. Following thorough investigation, evidenced-based clinical application is now established for a multitude of clinical settings to promote regeneration of periodontal hard tissues. Despite the large number of studies and review articles written on this topic, no single review has compiled the influence of EMD on tissue inflammation, an area of research that merits substantial attention in periodontology. The aim of the present review was to gather all studies that deal with the effects of EMD on tissue inflammation with particular interest in the cellular mechanisms involved in inflammation and soft tissue wound healing/resolution. The effects of EMD on monocytes, macrophages, lymphocytes, neutrophils, fibroblasts and endothelial cells were investigated for changes in cell behavior as well as release of inflammatory markers, including interleukins, prostaglandins, tumor necrosis factor-α, Matrix metalloproteinases and members of the OPG-RANKL pathway. In summary, studies listed in this review have reported that EMD is able to significantly decrease interleukin-1b and RANKL expression, increase prostaglandin E2 and OPG expression, increase proliferation and migration of T lymphocytes, induce monocyte differentiation, increase bacterial and tissue debris clearance, as well as increase fibroplasias and angiogenesis by inducing endothelial cell proliferation, migration and capillary-like sprout formation. The outcomes from the present review article indicate that EMD is able to affect substantially the inflammatory and healing responses and lay the groundwork for future investigation in the field.

  • Enamel Matrix Derivative improves gingival fibroblast cell behavior cultured on titanium surfaces.
    Clinical oral investigations, 2015
    Co-Authors: Yulan Wang, Yufeng Zhang, Dai Jing, Yang Shuang, Richard J Miron
    Abstract:

    Objective Although an extensive amount of research has demonstrated the positive effects of an Enamel Matrix Derivative (EMD) on soft tissue wound healing around intrabony defects, little information is available describing its effect on peri-implant soft tissues, an area that has recently gained tremendous awareness due to the increasing prevalence of peri-implantitis. The aim of the present study was to assess the role of EMD when gingival fibroblasts were cultured on titanium surface with different surface topographies.

  • Comparison of the Capacity of Enamel Matrix Derivative Gel and Enamel Matrix Derivative in Liquid Formulation to Adsorb to Bone Grafting Materials
    Journal of periodontology, 2015
    Co-Authors: Richard J Miron, Dieter D. Bosshardt, Daniel Buser, Yufeng Zhang, Anja C Gemperli, Michel Dard, Stefano Tugulu, Oana M. Caluseru, Fatiha Chandad, Anton Sculean
    Abstract:

    Background: The use of an Enamel Matrix Derivative (EMD) has been shown to enhance periodontal regeneration (e.g., formation of root cementum, periodontal ligament, and alveolar bone). However, in certain clinical situations, the use of EMD alone may not be sufficient to prevent flap collapse or provide sufficient stability of the blood clot. Data from clinical and preclinical studies have demonstrated controversial results after application of EMD combined with different types of bone grafting materials in periodontal regenerative procedures. The aim of the present study is to investigate the adsorption properties of Enamel Matrix proteins to bone grafts after surface coating with either EMD (as a liquid formulation) or EMD (as a gel formulation).Methods: Three different types of grafting materials, including a natural bone mineral (NBM), demineralized freeze-dried bone allograft (DFDBA), or a calcium phosphate (CaP), were coated with either EMD liquid or EMD gel. Samples were analyzed by scanning electr...

  • in vitro characterization of a synthetic calcium phosphate bone graft on periodontal ligament cell and osteoblast behavior and its combination with an Enamel Matrix Derivative
    Clinical Oral Investigations, 2014
    Co-Authors: Richard J Miron, Dieter D. Bosshardt, Daniel Buser, Anja C Gemperli, Michel Dard, Reinhard Gruber, Anton Sculean
    Abstract:

    Objectives Recent studies suggest that a combination of Enamel Matrix Derivative (EMD) with grafting material may improve periodontal wound healing/regeneration. Newly developed calcium phosphate (CaP) ceramics have been demonstrated a viable synthetic replacement option for bone grafting filler materials.

Dieter D. Bosshardt - One of the best experts on this subject based on the ideXlab platform.

  • The use of Enamel Matrix Derivative (Emd) for Treatment of Combined Apicomarginal Lesions in Apical Surgery: A Retrospective Analysis.
    European endodontic journal, 2018
    Co-Authors: Thomas Von Arx, Dieter D. Bosshardt
    Abstract:

    Objective: The outcome of apical surgery using modern techniques is favourable. However, the presence of a combined apicomarginal defect may negatively affect the postsurgical healing. The objective of this retro- spective analysis was to assess the healing of teeth with apicomarginal defects treated with apical surgery and Enamel Matrix Derivative (EMD). Methods: This retrospective study evaluated the application of EMD in apical surgery of 17 teeth with api- comarginal defects. Cases were followed for at least 1 year, and healing was classified based on established clinical and radiographic criteria. Results: The patient sample included nine females and eight males with a mean age of 50±18.2 years. Max- illary incisors (six lateral and four central) were the most frequently treated teeth. The majority of apico- marginal defects was located on the facial aspect of the root (70.6%) and belonged to defect class I (76.5%). Follow-up periods ranged from 1 to 5 years. Healing was successful in 14 teeth (82.4%). Conclusion: The application of EMD resulted in a similar outcome as in previously published clinical studies related to regenerative techniques for the treatment of apicomarginal defects in conjunction with apical surgery. Keywords: Apical surgery, Enamel Matrix Derivative, endo-perio lesion

  • Combination of Collagen Barrier Membrane with Enamel Matrix Derivative-Liquid Improves Osteoblast Adhesion and Differentiation.
    The International journal of oral & maxillofacial implants, 2017
    Co-Authors: Richard J Miron, Masako Fujioka-kobayashi, Dieter D. Bosshardt, Daniel Buser, Yufeng Zhang, Anton Sculean
    Abstract:

    PURPOSE: Collagen barrier membranes were first introduced to regenerative periodontal and oral surgery to prevent fast ingrowing soft tissues (ie, epithelium and connective tissue) into the defect space. More recent attempts have aimed at combining collagen membranes with various biologics/growth factors to speed up the healing process and improve the quality of regenerated tissues. Recently, a new formulation of Enamel Matrix Derivative in a liquid carrier system (Osteogain) has demonstrated improved physico-chemical properties for the adsorption of Enamel Matrix Derivative to facilitate protein adsorption to biomaterials. The aim of this pioneering study was to investigate the use of Enamel Matrix Derivative in a liquid carrier system in combination with collagen barrier membranes for its ability to promote osteoblast cell behavior in vitro. MATERIALS AND METHODS: Undifferentiated mouse ST2 stromal bone marrow cells were seeded onto porcine-derived collagen membranes alone (control) or porcine membranes + Enamel Matrix Derivative in a liquid carrier system. Control and Enamel Matrix Derivative-coated membranes were compared for cell recruitment and cell adhesion at 8 hours; cell proliferation at 1, 3, and 5 days; and real-time polymerase chain reaction (PCR) at 3 and 14 days for genes encoding Runx2, collagen1alpha2, alkaline phosphatase, and bone sialoprotein. Furthermore, alizarin red staining was used to investigate mineralization. RESULTS: A significant increase in cell adhesion was observed at 8 hours for barrier membranes coated with Enamel Matrix Derivative in a liquid carrier system, whereas no significant difference could be observed for cell proliferation or cell recruitment. Enamel Matrix Derivative in a liquid carrier system significantly increased alkaline phosphatase mRNA levels 2.5-fold and collagen1alpha2 levels 1.7-fold at 3 days, as well as bone sialoprotein levels twofold at 14 days postseeding. Furthermore, collagen membranes coated with Enamel Matrix Derivative in a liquid carrier system demonstrated a sixfold increase in alizarin red staining at 14 days when compared with collagen membrane alone. CONCLUSION: The combination of Enamel Matrix Derivative in a liquid carrier system with a barrier membrane significantly increased cell attachment, differentiation, and mineralization of osteoblasts in vitro. Future animal testing is required to fully characterize the additional benefits of combining Enamel Matrix Derivative in a liquid carrier system with a barrier membrane for guided bone or tissue regeneration.

  • Healing of localized gingival recessions treated with a coronally advanced flap alone or combined with an Enamel Matrix Derivative and a porcine acellular dermal Matrix: a preclinical study.
    Clinical oral investigations, 2015
    Co-Authors: Yoshinori Shirakata, Dieter D. Bosshardt, Anton Sculean, Yukiya Shinohara, Naoshi Takeuchi, Kotaro Sena, Kazuyuki Noguchi
    Abstract:

    Objective This study aimed to evaluate the effects of a porcine acellular dermal Matrix (PADM) with or without an Enamel Matrix Derivative (EMD) on gingival recession defects treated with a coronally advanced flap (CAF) in dogs.

  • Comparison of the Capacity of Enamel Matrix Derivative Gel and Enamel Matrix Derivative in Liquid Formulation to Adsorb to Bone Grafting Materials
    Journal of periodontology, 2015
    Co-Authors: Richard J Miron, Dieter D. Bosshardt, Daniel Buser, Yufeng Zhang, Anja C Gemperli, Michel Dard, Stefano Tugulu, Oana M. Caluseru, Fatiha Chandad, Anton Sculean
    Abstract:

    Background: The use of an Enamel Matrix Derivative (EMD) has been shown to enhance periodontal regeneration (e.g., formation of root cementum, periodontal ligament, and alveolar bone). However, in certain clinical situations, the use of EMD alone may not be sufficient to prevent flap collapse or provide sufficient stability of the blood clot. Data from clinical and preclinical studies have demonstrated controversial results after application of EMD combined with different types of bone grafting materials in periodontal regenerative procedures. The aim of the present study is to investigate the adsorption properties of Enamel Matrix proteins to bone grafts after surface coating with either EMD (as a liquid formulation) or EMD (as a gel formulation).Methods: Three different types of grafting materials, including a natural bone mineral (NBM), demineralized freeze-dried bone allograft (DFDBA), or a calcium phosphate (CaP), were coated with either EMD liquid or EMD gel. Samples were analyzed by scanning electr...

  • in vitro characterization of a synthetic calcium phosphate bone graft on periodontal ligament cell and osteoblast behavior and its combination with an Enamel Matrix Derivative
    Clinical Oral Investigations, 2014
    Co-Authors: Richard J Miron, Dieter D. Bosshardt, Daniel Buser, Anja C Gemperli, Michel Dard, Reinhard Gruber, Anton Sculean
    Abstract:

    Objectives Recent studies suggest that a combination of Enamel Matrix Derivative (EMD) with grafting material may improve periodontal wound healing/regeneration. Newly developed calcium phosphate (CaP) ceramics have been demonstrated a viable synthetic replacement option for bone grafting filler materials.

Michel Dard - One of the best experts on this subject based on the ideXlab platform.

  • Maxillary Sinus Floor Elevation Surgery With BioOss Mixed With Enamel Matrix Derivative: A Human Randomized Controlled Clinical and Histologic Study.
    The Journal of oral implantology, 2020
    Co-Authors: Séverine Vincent-bugnas, Yves Charbit, Michel Dard, Mathieu Charbit, Benjamin E. Pippenger
    Abstract:

    Xenograft bone substitutes are commonly used to increase bone volume and height in the deficient posterior maxilla. The addition of Enamel Matrix derivate (Emdogain) could increase the efficiency of the bone healing process. The aim of this prospective randomized, controlled split-mouth design study was to compare the percentage of newly formed bone in sinus floor augmentation with deproteinized bovine bone mineral with or without the addition of Enamel Matrix Derivative after 6 months of healing. Sixteen bilateral sinus floor augmentation procedures were performed. Deproteinized bovine bone mineral combined with Enamel Matrix Derivative (test) and deproteinized bovine bone mineral alone (control) groups were randomly allocated within each patient. Six months after augmentation and concurrent to implant placement, bone biopsies were taken for histomorphometric analysis. Additionally, implant survival and peri-implant bone levels were radiographically assessed at baseline and 24 months after functional loading. Histomorphometric analysis revealed a significantly higher amount of newly formed bone in the test group compared with the control group (22.6% and 15.5%, respectively; P = .033). No significant differences in the amount of remaining graft or connective tissue was found. Enamel Matrix Derivative added to deproteinized bovine bone mineral particles significantly increased new bone formation in sinus lift procedures in edentulous or partially edentulous patients with deficient bilateral posterior alveolar ridges requiring augmentation for implant placement.

  • Enamel Matrix Derivative inflammation and soft tissue wound healing
    Journal of Periodontal Research, 2015
    Co-Authors: Richard J Miron, Michel Dard, Miron Weinreb
    Abstract:

    Over 15 years have now passed since Enamel Matrix Derivative (EMD) emerged as an agent capable of periodontal regeneration. Following thorough investigation, evidenced-based clinical application is now established for a multitude of clinical settings to promote regeneration of periodontal hard tissues. Despite the large number of studies and review articles written on this topic, no single review has compiled the influence of EMD on tissue inflammation, an area of research that merits substantial attention in periodontology. The aim of the present review was to gather all studies that deal with the effects of EMD on tissue inflammation with particular interest in the cellular mechanisms involved in inflammation and soft tissue wound healing/resolution. The effects of EMD on monocytes, macrophages, lymphocytes, neutrophils, fibroblasts and endothelial cells were investigated for changes in cell behavior as well as release of inflammatory markers, including interleukins, prostaglandins, tumor necrosis factor-α, Matrix metalloproteinases and members of the OPG-RANKL pathway. In summary, studies listed in this review have reported that EMD is able to significantly decrease interleukin-1b and RANKL expression, increase prostaglandin E2 and OPG expression, increase proliferation and migration of T lymphocytes, induce monocyte differentiation, increase bacterial and tissue debris clearance, as well as increase fibroplasias and angiogenesis by inducing endothelial cell proliferation, migration and capillary-like sprout formation. The outcomes from the present review article indicate that EMD is able to affect substantially the inflammatory and healing responses and lay the groundwork for future investigation in the field.

  • Modified Tunnel Technique Combined with Enamel Matrix Derivative: A Minimally Invasive Treatment for Single or Multiple Class I Recession Defects.
    Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.], 2015
    Co-Authors: Séverine Vincent-bugnas, Yves Charbit, Julie Lamure, Patrick Mahler, Michel Dard
    Abstract:

    Purpose Gingival recession is a significant problem in the esthetic zone. Connective tissue grafts have been considered the gold standard, but they need a donor site that increases morbidity and discomfort for the patient.The purpose of the present study was to evaluate the clinical results of a modified tunnel technique that consists of replacing the connective tissue graft by Enamel Matrix Derivative, in the treatment of Miller Class I recession defects. Materials and Methods Twenty-six teeth in 14 subjects with Miller Class I recessions were treated using the tunnel procedure plus Enamel Matrix Derivative. The gingival recession, probing depth, clinical attachment level, and the width of the keratinized gingival tissue were recorded. Results There was a statistically significant reduction in gingival recession (88% of root coverage) and a gain in clinical attachment level (3.1 mm) between baseline and 24 months post-operatively, whereas the change in width of keratinized tissue and in probing depth was not statistically significant. All patients were satisfied with the esthetic appearance and would undergo the same surgery again. Conclusion This technique could be successfully used as an alternative to connective tissue grafts, with the advantage of avoiding the discomfort and morbidity of connective tissue harvesting. Clinical Significance This modified tunnel technique using Enamel Matrix Derivative potentially represents a clinically and esthetically satisfactory treatment of Miller Class I recession defects.

  • Comparison of the Capacity of Enamel Matrix Derivative Gel and Enamel Matrix Derivative in Liquid Formulation to Adsorb to Bone Grafting Materials
    Journal of periodontology, 2015
    Co-Authors: Richard J Miron, Dieter D. Bosshardt, Daniel Buser, Yufeng Zhang, Anja C Gemperli, Michel Dard, Stefano Tugulu, Oana M. Caluseru, Fatiha Chandad, Anton Sculean
    Abstract:

    Background: The use of an Enamel Matrix Derivative (EMD) has been shown to enhance periodontal regeneration (e.g., formation of root cementum, periodontal ligament, and alveolar bone). However, in certain clinical situations, the use of EMD alone may not be sufficient to prevent flap collapse or provide sufficient stability of the blood clot. Data from clinical and preclinical studies have demonstrated controversial results after application of EMD combined with different types of bone grafting materials in periodontal regenerative procedures. The aim of the present study is to investigate the adsorption properties of Enamel Matrix proteins to bone grafts after surface coating with either EMD (as a liquid formulation) or EMD (as a gel formulation).Methods: Three different types of grafting materials, including a natural bone mineral (NBM), demineralized freeze-dried bone allograft (DFDBA), or a calcium phosphate (CaP), were coated with either EMD liquid or EMD gel. Samples were analyzed by scanning electr...

  • in vitro characterization of a synthetic calcium phosphate bone graft on periodontal ligament cell and osteoblast behavior and its combination with an Enamel Matrix Derivative
    Clinical Oral Investigations, 2014
    Co-Authors: Richard J Miron, Dieter D. Bosshardt, Daniel Buser, Anja C Gemperli, Michel Dard, Reinhard Gruber, Anton Sculean
    Abstract:

    Objectives Recent studies suggest that a combination of Enamel Matrix Derivative (EMD) with grafting material may improve periodontal wound healing/regeneration. Newly developed calcium phosphate (CaP) ceramics have been demonstrated a viable synthetic replacement option for bone grafting filler materials.