The Experts below are selected from a list of 135 Experts worldwide ranked by ideXlab platform
Markus Muller - One of the best experts on this subject based on the ideXlab platform.
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strategies for the determination of cefazolin in plasma and microdialysis samples by short End Capillary zone electrophoresis
Electrophoresis, 2003Co-Authors: Bernhard X Mayer, Martina Petsch, Edda M Tschernko, Markus MullerAbstract:: Capillary zone electrophoresis was employed to determine cefazolin, a first-generation cephalosporin antibiotic, in plasma and microdialysis samples from patients. To shorten the analysis, the samples were injected from the short End of the Capillary, resulting in a separation time of < 3 min. Due to a high ionic strength of the biological matrices it was necessary to optimize the stacking conditions. For microdialysis samples a 1:10 dilution with water before injection was sufficient to obtain good peak shape. For plasma samples a protein removal step was required to obtain clean electropherograms and a good peak shape. Acetonitrile was used as precipitant resulting in an enhanced sample stacking in comparison to water dilution. The disadvantage of using acetonitrile was severe evaporation loss making quantitation impossible. A self-sealing film was used to seal each individual sample vial to suppress evaporation during long-term sequences. The calibration curves for spiked plasma and cefazolin in Ringer's solutions were linear in the range from 2-500 and 2.5-100 microg/mL, respectively. Limits of detection were 1.0 and 2.0 microg/mL in plasma and microdialysis samples, respectively. The assay was successfully applied to plasma and microdialysis samples obtained in vivo from the interstial space fluid of subcutaneous adipose and muscle tissue of patients undergoing cardiac surgery.
María José Medina-hernández - One of the best experts on this subject based on the ideXlab platform.
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High-throughput Capillary electrophoresis frontal analysis method for the study of drug interactions with human serum albumin at near-physiological conditions.
Electrophoresis, 2020Co-Authors: J.j. Martínez-pla, María Amparo Martínez-gómez, Yolanda Martín-biosca, Salvador Sagrado, Rosa María Villanueva-camañas, María José Medina-hernándezAbstract:The application of the short-End Capillary injection to Capillary electrophoresis frontal analysis (CE-FA) to study the interaction between basic, neutral and acid drugs towards human serum albumin (HSA) at near-physiological conditions is presented. The compounds selected display a wide range of binding affinities and the results obtained were in good agreement with those reported in the literature. An equation for the estimation of the number of primary binding sites and their corresponding affinity constants is developed isolating the experimentally measured variables in just one axis. The proposed CE-FA method to screen drug interactions with HSA under physiological conditions is simple, rapid and cost-effective what may facilitate its implementation in the drug discovery process.
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High‐throughput Capillary electrophoresis frontal analysis method for the study of drug interactions with human serum albumin at near‐physiological conditions
Electrophoresis, 2004Co-Authors: J.j. Martínez-pla, María Amparo Martínez-gómez, Yolanda Martín-biosca, Salvador Sagrado, Rosa María Villanueva-camañas, María José Medina-hernándezAbstract:The application of the short-End Capillary injection to Capillary electrophoresis frontal analysis (CE-FA) to study the interaction between basic, neutral and acid drugs towards human serum albumin (HSA) at near-physiological conditions is presented. The compounds selected display a wide range of binding affinities and the results obtained were in good agreement with those reported in the literature. An equation for the estimation of the number of primary binding sites and their corresponding affinity constants is developed isolating the experimentally measured variables in just one axis. The proposed CE-FA method to screen drug interactions with HSA under physiological conditions is simple, rapid and cost-effective what may facilitate its implementation in the drug discovery process.
Claudia Desiderio - One of the best experts on this subject based on the ideXlab platform.
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analysis of flavanone 7 o glycosides in citrus juices by short End Capillary electrochromatography
Journal of Chromatography A, 2005Co-Authors: Claudia Desiderio, Antonella Rossi, M SinibaldiAbstract:Abstract The separation of the major flavanone-7-O-glycoside constituents of Citrus was carried out by isocratic reversed phase Capillary electrochromatography using a 75 μm i.d. silica fused column packed with 5 μm ODS silica gel. In comparison to HPLC mode, Capillary electrochromatography resolution of flavanone glycosides was obtained with a high selectivity factor. Optimum separation conditions were found using a mixture of ammonium formate (pH 2.5)–acetonitrile (8:2, v/v) as the mobile phase by the short-End injection mode. Under these conditions all the investigated flavanones were baseline-resolved within short analysis time (i.e. between 5 and 10 min). A study, evaluating the intra- and inter-day repeatability as well as limit of detection and method linearity, was developed in accordance with the analytical procedures for method validation. The developed method was applied for the quantitative analysis of flavanone glycosides in commercial fruit juices (sweet orange, lemon and grapefruit).
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Application of Reversed Phase Short End-Capillary Electrochromatography to Herbicides Residues Analysis
Chromatographia, 2005Co-Authors: A. De Rossi, Claudia DesiderioAbstract:Reversed phase Capillary electrochromatography was applied to the determination of phenylurea herbicides residues in different samples of vegetables and vegetable processed food. The use of 40% acetonitrile mobile phase content provided a large elution window and strongly reduced the presence of matrix interferences in real samples. The compounds were baseline resolved within 14 min with 97612–113312 N/m range by using the short End injection method. The method was repeatable and linear and applied to the analysis of spiked soya milk and canned soya shoots samples extracted after a reduced sample pretreatment procedure. The analysis of vegetables showed some difficulties arising from the complexity and the strong variability of the matrix.
Shinichi Sekine - One of the best experts on this subject based on the ideXlab platform.
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rapid identification and quantitation for oral bacteria based on short End Capillary electrophoresis
Talanta, 2016Co-Authors: Jin Chen, Yi Ni, Yoshinori Yamaguchi, Qinmiao Chen, Shinichi SekineAbstract:Abstract High-speed Capillary electrophoresis (HSCE) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). In present study, the short-End Capillary electrophoresis coupled with one novel space domain internal standard method (SDIS) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f)). The reliability, reproducibility and accuracy properties of above mentioned SDIS method were investigated in detail. The results showed the target gene fragments of P.g, T.d and T.f could be precisely, fast identified and quantitated within 95 s via present short-End CE system. The analyte concentration and the ratio of space domain signals (between target sample and internal standard sample) featured a well linear relationship calculated via SDIS method. And the correlation coefficients R2 and detection limits for P.g, T.d, T.f genes were 0.9855, 0.9896, 0.9969 and 0.077, 0.114 and 0.098 ng/μl, respectively.
Yoshinori Yamaguchi - One of the best experts on this subject based on the ideXlab platform.
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rapid identification and quantitation for oral bacteria based on short End Capillary electrophoresis
Talanta, 2016Co-Authors: Jin Chen, Yi Ni, Yoshinori Yamaguchi, Qinmiao Chen, Shinichi SekineAbstract:Abstract High-speed Capillary electrophoresis (HSCE) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). In present study, the short-End Capillary electrophoresis coupled with one novel space domain internal standard method (SDIS) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (Porphyromonas gingivalis (P.g), Treponema denticola (T.d) and Tannerela forsythia (T.f)). The reliability, reproducibility and accuracy properties of above mentioned SDIS method were investigated in detail. The results showed the target gene fragments of P.g, T.d and T.f could be precisely, fast identified and quantitated within 95 s via present short-End CE system. The analyte concentration and the ratio of space domain signals (between target sample and internal standard sample) featured a well linear relationship calculated via SDIS method. And the correlation coefficients R2 and detection limits for P.g, T.d, T.f genes were 0.9855, 0.9896, 0.9969 and 0.077, 0.114 and 0.098 ng/μl, respectively.